The Bioactive Potential of Trawl Discard: Case Study from a Crinoid Bed Off Blanes (North-Western Mediterranean)

Although knowledge of the bioactive compounds produced by species inhabiting coastal waters is increasing, little is known about the bioactive potential produced by marine species occupying deeper habitats with high biodiversity and productivity. Here, we investigate about the bioactive potential of molecules produced by species that inhabit the crinoid beds, a poorly known essential fish habitat affected by trawling, wherein large amounts of commercial and noncommercial species are discarded. Based on a trawl survey conducted in 2019, 14% of the 64 species discarded on crinoid beds produce molecules with some type of bioactive potential, including; soft corals (Alcyonium palmatum); tunicates (Ascidia mentula); bony fish, such as horse mackerel (Trachurus trachurus); European hake (Merluccius merluccius); and chondrichthyans, such as small-spotted catshark (Scyliorhinus canicula). In addition, 16% of the discarded species had congeneric species that produce compounds with bioactive potential, indicating that such species might also possess similar types of bioactive molecules. Molecules with antioxidant, antitumour, antihypertensive, and antibacterial properties were the most frequent, which could provide the basis for future research aiming to discover new marine-based drugs and compounds for other human uses. Among all species or genera that produce compounds with bioactive potential, 68% presented medium or high vulnerability to trawling. Results show that the discarded catch contains many species, which produce different bioactive compounds that represent an added-value resource. These results highlight the importance of manage properly crinoid beds, to ensure that species that produce molecules with bioactive potential inhabiting these habitats are protected.     

Optimising the DPPH Assay for Cell-Free Marine Microorganism Supernatants

Antioxidants prevent ageing and are usually quantified and screened using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. However, this assay cannot be used for salt-containing samples, such as the cell-free supernatants of marine microorganisms that are aggregated under these conditions. Herein, the DPPH solvent (methanol or ethanol) and its water content were optimized to enable the analysis of salt-containing samples, aggregation was observed for alcohol contents of >70%. The water content of methanol influenced the activities of standard antioxidants but did not significantly affect that of the samples. Based on solution stability considerations, 70% aqueous methanol was chosen as the optimal DPPH solvent. The developed method was successfully applied to the cell-free supernatants of marine bacteria (Pseudoalteromonas rubra and Pseudoalteromonas xiamenensis), revealing their high antioxidant activities. Furthermore, it was concluded that this method would be useful for the screening of marine microorganism–derived antioxidants, which also has numerous potential applications, such as salt-fermented foods.     

Spray Drying of Chitosan Acid Salts: Process Development,Scaling Up and Physicochemical Material Characterization

We investigated a spray drying process for preparing water-soluble salts of high molecular weight chitosan (CH) intended for pharmaceutical excipient applications. CH was derived from chitin of marine lobster origin (Panulirus argus). The effects of organic acid (acetic or lactic acid) and the ratio (difference) of inlet/outlet air temperature (140/90 °C or 160/100 °C) on spray drying were studied. The yield of spray-dried CH salt powders ranged from 50% to 99% in laboratory and industrial-scale processes. The spray-dried dry powder of CH salts consisted of spherical agglomerated particles with an average diameter of 36.2 ± 7.0 µm (CH acetate) and 108.6 ± 11.5 µm (CH lactate). After dispersing the spray-dried CH salt powder samples in purified water, the mean particle sizes obtained for the CH acetate salts were 31.4 nm (batch A001), 33.0 nm (A002) and 44.2 nm (A003), and for the CH lactate salts 100.8 nm (batch L001), 103.2 nm (L002) and 121.8 nm (L003). The optimum process conditions for spray drying were found: an inlet air temperature of 160 ± 5 °C, an outlet temperature of 100 ± 5 °C and an atomizer disk rotational speed of 18,200 min⁻¹. The X-ray powder diffraction (XRPD) and differential scanning calorimetry (DSC) results confirmed the amorphous state of the CH salts. The ¹H nuclear magnetic resonance (NMR) and Fourier transform infrared (FT-IR) spectra of CH acetate and lactate salts verified that the spray drying process does not affect the polymer backbone. In conclusion, both laboratory and industrial-scale spray drying methods for preparing water-soluble acid salts of CH are reproducible, and the physicochemical properties of the corresponding CH acid salts are uniform.     

Genome Mining for Antimicrobial Compounds in Wild Marine Animals-Associated Enterococci

New ecosystems are being actively mined for new bioactive compounds. Because of the large amount of unexplored biodiversity, bacteria from marine environments are especially promising. Further, host-associated microbes are of special interest because of their low toxicity and compatibility with host health. Here, we identified and characterized biosynthetic gene clusters encoding antimicrobial compounds in host-associated enterococci recovered from fecal samples of wild marine animals remote from human-affected ecosystems. Putative biosynthetic gene clusters in the genomes of 22 Enterococcus strains of marine origin were predicted using antiSMASH5 and Bagel4 bioinformatic software. At least one gene cluster encoding a putative bioactive compound precursor was identified in each genome. Collectively, 73 putative antimicrobial compounds were identified, including 61 bacteriocins (83.56%), 10 terpenes (13.70%), and 2 (2.74%) related to putative nonribosomal peptides (NRPs). Two of the species studied, Enterococcus avium and Enterococcus mundtti, are rare causes of human disease and were found to lack any known pathogenic determinants but yet possessed bacteriocin biosynthetic genes, suggesting possible additional utility as probiotics. Wild marine animal-associated enterococci from human-remote ecosystems provide a potentially rich source for new antimicrobial compounds of therapeutic and industrial value and potential probiotic application.     

Soil erosion modelling of burned and mulched soils following a Mediterranean forest wildfire

Soil erosion modelling applied to burned forests in different global regions can be unreliable because of a lack of verification data. Here, we evaluated the following three erosion models: (1) Water Erosion Prediction Project (WEPP), (2) Morgan-Morgan-Finney (MMF) and (3) Universal Soil Loss Equation-Modified (USLE-M). Using field plots that were either untreated or mulched with straw, this study involved observations of soil loss at the event scale at a burned pine forest in Central Eastern Spain. The erosion predictions of the three models were analysed for goodness-of-fit. Optimization of the MMF model with a new procedure to estimate the C-factor resulted in a satisfactory erosion prediction capacity in burned plots with or without the mulching treatment. The WEPP model underestimated erosion in the unburned areas and largely overestimated the soil loss in burned areas. The accuracy of soil loss estimation by the USLE-M model was also poor. Calibration of the curve numbers and C-factors did not improve the USLE-M model estimation. Therefore, we conclude that an optimized MMF model was the most accurate way to estimate soil loss and recommend this approach for in Mediterranean burned forests with or without postfire mulching. This study gives land managers insight about the choice of the most suitable model for erosion predictions in burned forests.     

Water and nutrient uptakes efficiencies in rosemary plants under different fertigation treatments

The aim of this work was to evaluate the effects of different fertigation treatments: T₀ (control treatment), T₁ (raw leachates from C. melo) and T₂ (raw leachates from C. melo blended with tap water (50/50% v/v)) on plant growth, nutrient concentration in leachate and water and nutrient use efficiencies of rosemary. At the end of experiment, rosemary plants fertigated with leachates decreased the total dry weight and the water use efficiency compared to the control treatment. The fertigation with leachates from C. melo affected the values of the nutrient concentrations in leachates from rosemary plants during the experimental period and improved the nitrogen (NUE) and phosphorus (PUE) use efficiencies compared to the control treatment. Our results indicate that reuse of horticultural leachates for the fertigation of rosemary plant in containers assumes a water and nutrient savings, but from an environmental point of view, there is a risk of pollution.     

Effect of salinity on metalloenzymes of oxygen metabolism in two leguminous plants

Abstract

Superoxide dismutase (SOD) pattern, catalase, Cyt c oxidase and fumarase activity were studied in leaves of Phaseolus vulgaris and Vigna unguiculata plants growth in two sodium chloride (NaCl) concentrations (35 mM and 100 mM). In bean plants growth with NaCl, leaf chloride (Cl^?) contents were higher than in control plants, and the same was found for sodium (Na⁺) and potassium (K⁺) contents, although to a lesser degree. In cowpea leaves, Na⁺ and Cl^? had a similar increase due to salt‐growth conditions. Under salinity, all changes in the antioxidant (SOD and catalase) enzymes levels were smaller in bean than in cowpea plants. In Phaseolus at 15 days growth, Cu, Zn‐SOD I showed an increase by the effect of salt treatment, but this induction did not occur at 30 days growth, and both Mn‐SOD and Cu, Zn‐SOD II did not show variations due to salt‐stress. In Vigna, Mn‐SOD was decreased by salinity but this was compensated by an increase in Cu, Zn‐SOD I activity in plants at 30 days growth, whereas in young leaves under saline conditions, both isozymes were also decreased. Likewise, there was a rise in cytochrome c oxidase and fumarase activity in leaves of NaCl‐treated plants compared to the control. The activity changes observed are discused in term of their possible relevance to plant sensitivity to saline conditions.     

Nitrogen use efficiency and nutrient partitioning in maize as affected by blends of controlled-release and conventional urea

ABSTRACT

Blends of controlled-release urea (CRU) and conventional urea can be an alternative to conventional fertilization to improve nitrogen use efficiency (NUE) and reduce costs when applied as a single application to agricultural crops. Different indexes of NUE, grain yield, nutrient uptake and partitioning in maize (Zea mays L.) were investigated in field experiments. The treatments consisted of a single rate of 180 kg N ha^?1 with different proportions of polymer-sulfur coated urea (PSCU) and conventional urea (U) applied incorporated at sowing (0.05 m below and 0.1 m to the side of the seed row) at two tropical sites (Site 1, Typic Haplustox; Site 2, Rhodic Haplustox) in Brazil. A control treatment (without urea-N) and a treatment with conventional urea management (UCM: 20% of urea-N applied as basal fertilizer and 80% of N applied as top dressing) were also included. This study demonstrates that blends of PSCU and U are efficient in supplying N throughout the maize cycle at a Typic Haplustox site when applied in a single application incorporated at sowing, resulting in high yields and adequate macronutrient uptake. PSCU improved NUE index compared to U and UCM. There was not response for N fertilization in the Rhodic Haplustox site.     

Colored plastic mulches affect soil temperature and tuber production of potato

Abstract

The main crops on which plastic mulch is widely used in Mexico include tomato, bell pepper, eggplant, melons, watermelons, and strawberry; however, very little research has been performed on potato. One of the main benefits associated with plastic mulching is the modification of the microclimate around the plant. To obtain a positive microclimate modification studies are required to understand how plastic mulch affects growth and yield of a given species. Previous field research assessing the response to colored plastic mulching in potato shows no consistent results on yield, maybe because it has been performed under different geographical latitudes. Potato in Mexico is conventionally cultivated on bare soil combined with various irrigation systems. The objective of the present study was to examine the effect of colored plastic mulches on soil temperature, growth, yield and photosynthetic response of potato plants. The experiment was conducted in Northeast Mexico and the treatments included were: black plastic mulch (BPM); white-on-black plastic mulch (W/B), silver-on-black (SPM), aluminum-on-black plastic mulch (APM) and a control that consisted of bare soil cultivated plants. Treatments were arranged in a randomized complete block design with four replications. Results suggest that average daily mean soil temperature was linearly and negatively correlated with total yield and yield of first-quality tubers. Total yield and yield of first-quality tubers of plants mulched with W/B, SPM and APM was significantly higher (p≤0.05) than those of control plants. Leaf area and shoot dry weight were increased when soil temperatures were higher due to the effect of radiation transmission to the soil, however, this decrease was associated with a decrease in tuber production. The results of our study indicate that plants grown under BPM, which induced the highest soil temperature, showed marginal difference in yield compared with control plants, suggesting that colored plastic mulches have a positive effect on yield through decreased rise in soil temperature.