新世纪重庆蚕丝业发展问题与对策

重庆蚕丝业在 2 0世纪 80年代末期至 90年代中期居全国第四位 ,不仅是重庆出口创汇的重要支柱 ,也是财政和蚕农收入的重要来源。我国“入世”后 ,蚕丝业是重要的受益行业 ,面临巨大的市场需求与发展空间。根据中央西部大开发战略 ,结合我国丝绸产业结构调整、东桑西移的历史机遇和重庆得天独厚的茧丝绸资源 ,就新世纪重庆蚕丝业发展存在的问题和应采取的措施谈几点意见。1　重庆蚕丝业的发展现状及问题1 .1　现状在重庆 40个区县 (市 )行政区划中 ,有养蚕的区县 (市 ) 35个 ,蚕种场 2 7个 ,蚕种冷库6座 ,年制种能力 1 30余万张、年冷藏能力2…     

草原的生态系统服务:Ⅲ.价值和意义

连续综述了中国草原／草地生态系统服务价值的构成,草地生态系统服务的总价值,各类型草地的生态系统服务的价值。论证了草原／草地为中国提供了最大的生态系统服务价值,草地生态系统服务的生态价值远大于经济价值。论述了草地生态系统服务价值评估具有下列重要意义：1．指明了草地生态系统服务的具体项目及其不可代替性;2．确定了草地生态资本的价值;3．促进了环境成本核算和实现绿色GDP。文章在最后还讨论了草原／草地生态系统服务价值评估中存在的问题。     

Structural and metabolic integrity of sheep external intercostal muscle during extended culture

The objectives of this study were to examine the structural and metabolic integrity of isolated sheep external intercostal muscle bundles following variable lengths of preincubation (0 to 192 h). Samples of intact external intercostal muscle (10 to 15 g), with tendons attached, were prepared from growing wethers and maintained at their resting lengths during preincubation for 0 to 192 h. Protein synthesis (PS), protein degradation (PD), acetate oxidation and ultrastructural integrity of muscle samples were examined at 0 to 192 h, 0 to 96 h, 0 to 48 h and 0 to 96 h following isolation, respectively. Additionally, the effects of variable fetal calf serum (FCS) concentrations (0 to 20%; w/v) on PS and PD and acetate oxidation were examined. Rate of PS increased as preincubation time increased to 192 h; however, most of this increase was due to the proliferation of fibroblasts on the surface of the muscle sample. Addition of cytosine arabinoside to the incubation media prevented the fibroblast-dependent increase in PS; however, it did not entirely prevent the preincubation time-dependent increase in PS. Rate of PD increased greatly upon preincubation. The nitrogen balance of incubated muscles was negative at all times examined. Acetate oxidation was maintained through 12 h of preincubation and thereafter declined. Relatively normal myofibrillar structure was maintained through 48 h of preincubation; however, loss of mitochondrial integrity and dissolution of Z-disks at 48 h and at 96 h of preincubation were evident. Isolated tissues were able to respond to FCS concentration in medium following 48 h of preincubation.     

德菲尔法构建重大动物疫病预警指标体系

[目的 ]构建重大动物疫病暴发流行预警指标体系。[方法 ]以禽流感和口蹄疫为例,采用文献调研法和德菲尔专家咨询法中的专家会议法初拟重大动物疫病预警体系的框架和指标;应用德菲尔专家咨询法评价预警指标的重要性、可操作性;应用组合权重法比较各指标综合影响的大小,并将指标分类。[结果 ]筛选出禽流感和口蹄疫预警预报一级指标6项(易感动物、饲养管理和免疫情况等)以及二级指标14项(免疫效果、环境消毒、温度等),并将指标分为三类(关键指标、重要指标和一般指标)。[结论 ]初步构建了重大动物疫病暴发流行预警指标体系。     

Systematic Review of Brain Tumor Treatment in Dogs

Intracranial neoplasia is commonly diagnosed in dogs and can be treated by a variety of methods, but formal comparisons of treatment efficacy are currently unavailable. This review was undertaken to summarize the current state of knowledge regarding outcome after the treatment of intracranial masses in dogs, with the aim of defining optimal recommendations for owners. This review summarizes data from 794 cases in 22 previously published reports and follows PRISMA guidelines for systematic review. A Pubmed search was used to identify suitable articles. These then were analyzed for quality and interstudy variability of inclusion and exclusion criteria and the outcome data extracted for summary in graphs and tables. There was a high degree of heterogeneity among studies with respect to inclusion and exclusion criteria, definition of survival periods, and cases lost to follow‐up making comparisons among modalities troublesome. There is a need for standardized design and reporting of outcomes of treatment for brain tumors in dogs. The available data do not support lomustine as an effective treatment, but also do not show a clear difference in outcome between radiotherapy and surgery for those cases in which the choice is available.     

狂犬病病毒糖蛋白转基因小鼠的构建研究

将狂犬病病毒糖蛋白ｃＤＮＡ ＢｇｌⅡ（１．６７ｋｂ）片段正向插入ｐＭＴ０１０／Ａ＾＋ＢａｍＨⅠ切点，构建重组质粒ｐＭＴ０１０／Ａ＾＋－Ｒｇｐ，用ＥｃｏＲⅠ＋ＳａｌⅠ对ｐＭＴ０１０／Ａ＾＋－Ｒｇｐ进行双酶切后，回收含有狂犬病病毒糖蛋白ｃＤＮＡ和绵羊ＭＴ启动子的２．７６ｋｂ片段，通过显微注射技术将该片段注入小鼠单细胞受精卵雄前核内，在进行胚胎移植后，获得４４只小鼠，经ＰＣＲ、Ｓｏｕｔｈｅｒｎ杂交及原位     

广东果园种草养鹅的潜力与对策

本文分析了广东果园种草养鹅的潜力,从果园草地的选择、人工草地建立、草地利用时间、利用方法、围栏轮牧、载鹅量、草地管理等方面提出了果园种草养鹅的对策。     

河北进境种用动物衣原体血清学检测

衣原体病是一种重要的人畜共患病,给养殖业带来巨大的经济损失,同时严重威胁从业人员的身体健康。本研究对河北辖区引进的2批种鸡、2批种鸭及2批种猪进行抽样,使用间接血凝试验（IHA）检测引进种用动物的衣原体抗体。结果表明：衣原体IHA抗体阳性率为15.7%。在种鸡、种鸭和种猪中均检测到衣原体IHA抗体,阳性率分别为18.1%、4.2%和20%。这说明在引进种用动物中存在着较高的衣原体抗体阳性率,需要加强对该病的检测。     

浅议吸收国际标准应重视标准的经济和贸易属性

我国是畜牧业生产大国,畜禽饲养总量已连续多年稳居世界前列,如何将这种生产优势尽快转化为贸易优势和经济优势是我国加入WTO之后畜牧业发展面临的重要问题。我国近年来参与国际贸易竞争的情况表明,以卫生质量标准为表现形式的技术性贸易壁垒是制约畜产品出口的主要障碍。根据WTO相关规则,采用国际标准是突破这种障碍的基本途径之一。本文通过介绍畜产品贸易中的国际标准及我国的采标情况,从标准的经济和贸易属性出发,对采用国际标准的问题进行探讨,以期对我国今后的采标工作有所帮助。1畜产品贸易中的国际标准在畜产品国际贸易协调中发…     

甘肃省张掖市动物检疫电子出证实践中的问题分析

为确保动物检疫工作及时有效,探索电子出证工作及系统管理的经验,本文分析了甘肃省张掖市动物检疫电子出证工作中存在的一些问题,如票证作废率高、不按顺序使用票证、票证填写不规范、签名不符合要求等,提出了严格票证管理、规范电子出证工作流程、加强电子出证人员的再培训、落实责任追究等建议。