论养蜂业在农业中的地位和作用

本文从农业增产、环境可持续发展、环境监控和治理、守护人类健康、建设"节约型社会"、农民增收、蜂产品出口创汇等方面综述了蜂业在我国农业中的地位和作用,阐述了我国养蜂业在世界的地位,并对今后的发展潜力和趋势进行了预测。     

鹅掌楸及其在园林绿化中的应用

鹅掌楸树形端直、叶形奇特、花朵酷似郁金香,是世界著名的观赏树种,利用历史悠久。依据相关文献概述了鹅掌楸的种质类型、分布特征,总结了其文化内涵和生态习性,重点阐述了鹅掌楸在园林绿化中的应用,并对进一步开发利用进行了展望。     

不同紫花苜蓿品种引种试验研究

引进了国外8个苜蓿品种,在2003年至2005年对其产量、株高、营养价值、越冬率、越夏性、抗倒伏和经济效益等方面进行了比较,结果表明这8个苜蓿品种在郑州地区均表现了良好的适应性,其中WL232和猎人河是推广的首选品种。     

半导体发光二极管(LED)的应用及发展前途

半导体二极管(LED)是应用最为广泛的电子元件之一,本文介绍了半导体二极管(LED)的发光原理、特性、分类、检测、应用及发展前景。     

道德失范现象的制度因素及对策研究

道德和谐是建设社会主义和谐社会的根本要求.当前,社会生活中的道德失范现象严重制约了和谐社会建设进程.道德失范现象直接根源于利益分配中的制度性不公:一是直接的行为奖惩制度缺失;二是相关利益分配制度的不合理.道德是建立在一定利益基础之上的,其核心本质是利益的公正分配.因此,构建"德得恶失"的利益激励制度,完善公平的利益分配制度,是改善道德失范现象、推进和谐社会建设的根本.     

基因工程猪γ-干扰素对PRRSV和SIV混合感染猪的预防效果试验

应用基因工程猪γ-干扰素和血清对人工感染PRRSV(繁殖与呼吸综合征病毒)和SIV(猪流感病毒)仔猪的预防试验，结果表明猪γ-干扰素预防两种病毒混合感染效果显著，感染前和感染后肌肉注射5万单位猪的发病率分别下降71．2％和60％，猪γ-干扰素和特异性抗体联合使用效果最好，可100％预防病毒感染，表明干扰素和抗体在抗病毒感染方面起到协同作用。本试验不仅为PRRSV和SIV混合感染提供了良好的预防方法，还将对病毒病的预防和治疗起到借鉴作用。     

Leaf photosynthesis is enhanced in normal oil maize pollinated by high oil maize hybrids

The grain yield of normal oil maize (Zea mays L.) might increase when pollinated by high oil maize (HOM) hybrids because of heterosis. To testify that the grain yield increase might be a result of improved photosynthetic rate and related traits, the normal oil maize (NOM) hybrid, Nongda108, was cross-pollinated by three HOM hybrids, HOM202, HOM115 and HOM4515 (for short as ND108pHOM202, ND108pHOM115 and ND108pHOM4515). We found that the ND108pHOM202 and ND108pHOM115 exhibited higher net photosynthetic rate (P_n), accompanied by larger stomatal conductance (g_s) and transpiration rate (E). Moreover, delayed leaf senescence was observed in their leaves, including larger leaf area index (LAI) and higher Chl content and Chl a/b ratio. Apart from higher phosphoenolpyruvate carboxylase (PEPCase) activity, the soluble proteins were also higher in the two cross-pollinations. The higher leaf photosynthesis could explain the grain increase in ND108pHOM202 and ND108pHOM115. However, ND108pHOM4515 exhibited a decreased photosynthetic characteristic and yield performance. Significantly positive relation between grain yield and biomass (r² = 0.96, P < 0.05), P_n and biomass (r² = 0.74, P < 0.05) also suggested that the yield increase in the two cross-pollination treatments was generally owing to the higher photosynthetic rate and related photosynthetic traits.     

仔猪色氨酸研究新进展

<正>色氨酸(Trp)是仔猪日粮中排于赖氨酸、苏氨酸、蛋氨酸之后的第4限制性氨基酸。它除了有助于蛋白质沉积,最重要的作用就是对适口性和采食量的调     

亚洲黑熊COI序列变异及其DNA条码的应用

利用DNA编码技术对亚洲黑熊东北亚种和指名亚种不同个体进行DNA编码,结合Genbank中亚洲黑熊台湾亚种和四川亚种以及熊科相关物种的序列构建系统发育树,并与12SrRNA基因进行比较。研究结果表明,亚洲黑熊COI基因变异显著,各亚种分化明显。系统树显示,亚洲黑熊和美洲黑熊遗传分化明显,且不是姐妹群;北极熊和棕熊关系较近,为姐妹群。COI作为DNA条码在研究亚洲黑熊的分类地位和进化关系上是有效的。在解决亚种分化及关系时,COI基因比12SrRNA基因更有效。     

Protein extraction from rice (<Emphasis Type="Italic">Oryza sativa</Emphasis> L.) root for two-dimensional electrophresis

An efficient protein extraction method for two-dimensional gel electrophoresis (2-DE) from plant samples is usually challenging due to the low protein content and high level of interfering compounds. Proteomic analyses of rice (Oryza sativa L.) roots are limited by the lack of an efficient protein extraction method. To establish an effective protocol of protein extraction suitable for 2-DE analysis in rice roots, we evaluated three protein extraction methods (trichloroacetic acid [TCA]/acetone, Mg/NP-40/TCA, and tris-base/acetone). Our results showed that the Mg/NP-40/TCA extraction method had the highest protein yield and is the best resolution of protein separation among the three methods. The TCA/acetone method exhibited clear protein profiles and detected more protein spots with the highest intensity in the region of high Mr (above 45 kDa) than the other methods. However, this method was unable to detect proteins with low-Mr (less than 24.0 kDa). The Tris-base/acetone method showed the poorest resolution of protein separation. Our results suggest that the Mg/NP-40/TCA method was the most effective among the three methods and may provide enhanced proteomic information for rice and other crop roots.