Bioassay evaluation of the systemic properties of carboxin in lemon seedlings

Carboxin was toxic to the fungus Deuterophoma tracheiphila. Uptake and translocation of carboxin (2,3-dihydro-6-methyl-5-phenylcarbamoyl-1,4-oxathiin) in lemon seedlings were assessed in plant extracts by a bioassay technique. Root uptake and translocation to stem and leaves were demonstrated from both an aqueous solution and drenched soil. Leaf uptake was lower than root uptake, and could be enhanced by removing the epicuticular wax. Chromatographic tests suggested that the compound responsible for fungitoxicity within plant tissues may have been carboxin itself.     

Effectiveness of antigens from various developmental stages of Ascaris suum in the deomonstration of the migratory phase of experimental ascariasis in pigs]

Tests of the efficiency of antigens prepared from different developmental stages of Ascaris suum in indirect haemagglutination test in the course of proving the migration phase of experimental ascariasis in pigs show that the antigens prepared by ultrasound from the invasive larval stage of A. suum in comparison with antigens of sexually mature stages have higher serological activity. By using this antigen it is possible to prove specific antibodies in experimentally invaded pigs from 6 to 120 days after invasion as opposed to the other tested antigens (the detectability of antibodies from the 7th-8th day to the 64th day after invasion).     

Developmental stages of Taenia krabbei Moniez, 1879 (Cysticercus tarandi) in deer (Capreolus capreolus L.) in Czechoslovakia]

The breast and shoulder-blade muscles of a roe (Caprelous capreolus L.), shot in a hunting district in western Bohemia, were found to contain cysticerci with morphological characters and size corresponding to those of Cysticercus tarandi, which is a developmental stage of the tapeworm Taenia krabbei Moniez, 1879. The surface as well as deeper layers of muscle contained one to two cysticerci per 100 cm2 of area. The authors describe the larvocysts and histological changes in the site where the micro-organisms were found. The cysticerci are discussed as to the species to which they belong.     

Two Hungarian isolates of cucumber mosaic virus from sweet pepper (Capsicum annuum) and melon (Cucumis melo): Identification and antiserum preparation

Two Hungarian virus isolates from sweet pepper (K8) and melon (S4) were identified as cucumber mosaic virus (CMV) on the basis of host plant reactions and serology. The isolates were purified and antisera prepared. Homologous antiserum titers in double-diffusion tests were 256 (K8) and 512 (S4). They were serologically closely related to each other and to other CMV isolates. On the basis of symptoms they belong to different symptomatological groups of CMV; this was supported by serological properties. Sedimentation coefficients were c. 93 S, at 2 mg ml^–1. Purified preparations, stained with 2% uranyl acetate, showed spherical particles. In ELISA purified preparations reacted with each other's antisera.Samenvatting Twee hongaarse virusisolaten uit paprika (K8) en meloen (S4) werden geïdentificeerd als komkommermozaïekvirus (CMV) met behulp van toetsplanten en serologie. Beide isolaten werden gezuiverd en er werden antisera tegen bereid. De homologe titers van de antisera in de agar-geldiffusietoets bedroegen 256 (K8) en 512 (S4). K8 en S4 waren serologisch nauw verwant aan elkaar, evenals aan andere CMV-isolaten. Op grond van hun symptomen op toetsplanten behoren ze tot verschillende symptomatologische groepen van CMV. Dit laatste werd gesteund door de serologische eigenschappen. Beide isolaten hebben een sedimentatiecoëfficiënt van ca 93 S, bij een concentratie van 2 mg ml^–1. Gezuiverde preparaten, gekleurd met 2% uranylacetaat, bleken bolvormige deeltjes te bevatten. In ELISA reageerden gezuiverde preparaten van K8 en S4 met elkaars antisera.