土壤棉黄萎病菌选择性培养基——棉选一号

 棉花黄萎病的发生发展与土壤中微菌核的数量有着密切关系.本文介绍一种用于检验土壤内棉黄萎病菌的选择性培养基——棉选一号.其成份为:NaNO₃ 2g、MgSO₄·7H₂O 0.5g、K₂HPO₄ 1g、Fe₂（SO₄）₃·XH₂O 0.01g.KCl 0.5g、蔗糖5g、琼脂15g、蒸馏水1000ml、氯霉素300mg、井岗霉素2.5ppm、五氯硝基苯350ppm、克菌丹0.5ppm.这种培养基能有效地分离自然土壤中黄萎病菌的微菌核,而极少受其他微生物的干扰,由于培养基上菌落大、黑色、微菌核呈放射状,故易用肉跟计数微菌核的数量.     

检测植物病毒ELISA异种动物抗体双夹心法的研究和应用

 制备南方菜豆叶花叶病毒、大豆花叶病毒、烟草花叶病毒、番茄不孕病毒、苜蓿花叶病毒的兔抗血清和鼠腹水抗体,组合成ELISA异种动物抗体双夹心试验。抗体球蛋白的适宜工作浓度为4微克/毫升;未经纯化的特异抗血清(抗体)的适宜工作浓度为10^-4(即1/10,000)稀释度。检测以上5种病毒的灵敏度:提纯抗原为10-0.64毫微克/毫升;病叶澄清液为1/10,000-1/100,000稀释度。本法灵敏度与ELISA双抗体夹心法相当或略高。一般8小时内可得出结果。适用于大量样品的检测。     

克里雅河流域沉积物的粒度特征

克里雅河流域由普鲁至米萨来,冲积沙粒径向细递减;沙丘沙一般粗于下伏沙,而冲、洪积下伏地层上的沙丘风成沙则细于下伏沙;高大沙丘粒级组合主要受就近沙源影响。用累计百分数和粒级的φ值作图,求得φ_1、φ_5、φ_(16)、φ_(25)、φ_(50)、φ_(75)、φ_(84)、φ_(95)等8个数值,输入计算机获得最大粒径、中值粒径、平均粒径(M_z)、标准离差(σ_1)、偏度(S_(K1))、峰态(K_G)等值。     

Characterization of polymorphisms of transferrin receptor and their association with susceptibility to ETEC F4ab/ac in pigs

Diarrhoea caused by enterotoxigenic Escherichia coli (ETEC) expressing F4 (F4ab, F4ac and F4ad) fimbriae is a significant cause of mortality and morbidity in newborn and weaned pigs. The locus controlling susceptibility towards ETEC F4ab/ac has been mapped to SSC13q41, in which TFRC (transferrin receptor) was localized and considered as a positional candidate gene for ETEC F4ab/ac receptor. In this study, we determined susceptibility/resistance to ETEC F4ab/ac in a total of 755 F2 animals from a White Duroc x Erhualian intercross using a microscopic enterocyte adhesion assay. We identified two TFRC polymorphisms (SNPs 591 A>G and 632 A>G) in a single exon after comparative sequencing analysis of 2371-bp amplicons containing the complete coding region of TFRC using RNA of eight full-sib F2 animals with susceptible and resistant phenotypes. The intron sequences flanking the two exon polymorphisms were obtained, revealing an intron polymorphism (SNP 291 C>T). We genotyped the 19 founder animals of the White Duroc x Erhualian intercross for the identified polymorphisms, showing that only the 291 C>T polymorphism is a highly informative marker. We further genotyped all 59 F1 and 755 F2 animals for the 291 C>T polymorphism, and the association of this polymorphism with susceptibility/resistance to ETEC F4ab/ac in these F2 animals was evaluated by the transmission disequilibrium test. The result showed that the 291 C>T polymorphism is not a causal mutation, however, has a significant linkage disequilibrium with the ETEC F4ab/ac, especially F4ac receptor locus.     

陶赛特肉羊与草地型藏系绵羊杂交效果初报

草地型藏系绵羊属我国西藏绵羊品种之一,在甘南地区的优良品种有草地类型的欧拉羊、甘加羊、乔科羊,它们生长在海拔2 800～3 500 m之间,具有抗寒、耐粗饲、遗传性稳定、产肉性能良好的特点.但由于粗放的生产经营方式和超载过牧,使羊只生产性能逐渐下降.……     

Effects of mitochondrial ATP-sensitive potassium channel opener and hypoxic preconditioning on the hyperpolarization-activated current of sinoatrial node cells in neonatal rats

AIM: To compare the effects of hypoxic preconditioning (HP) and mitochondrial ATP-sensitive potassium (K_ATP) channel opener pretreatment on the hyperpolarization-activated current (I_f) in sinoatrial node cells.METHODS: Sinoatrial node cells were randomized to five groups: ① Control;②Hypoxia/reoxygenation (H/R);③ HP;④ Diazoxide (mitochondrial K_ATP channel opener)+H/R;⑤ 5-HD (mitochondrial K_ATP channel blocker)+HP.At the end of the experiment, I_f was recorded by whole-cell patch clamp technology.RESULTS: ①H/R significantly enhanced the current densities of I_f, shifted the current activation curve to more positive value by changing the half-activation voltage from (-98.9±2.4)mV to (-85.2±2.5) mV (P＜0.01).② Both diazoxide pretreatment and HP remarkably reduced the augmented current density caused by H/R and shifted the current activation curve to more negative value by changing the half-activation voltage to (-90.7±5.0) mV (P＜0.01) and (-92.2±1.9) mV (P＜0.01).③ 5-HD pretreatment blocked the effects of HP and reversed the half-activation voltage to (-86.3±2.7) mV (P＜0.01).CONCLUSION: The study demonstrates that both mitochondrial K_ATP channel opener pretreatment and HP make current density and kinetics of I_f to approach normal level and to maintain electrophysiological stability of sinoatrial node cells during H/R.     

荔枝常温贮藏与包装的研究

研究了以包装为主题的荔枝常温贮运技术,结果表明简单的塑料框十树叶包装可减少果实失重达10．4％,果实贮藏3d商品率为96．20％,5d为71．47％。同时上述包装结合防腐保鲜处理,贮藏5d果实商品率为88．2％,提高了16．8％,而失重降低了2．2％。进行了泡沫箱的加冰包装的荔枝常温贮运试验,测定了箱内温度、CO2、O2和乙烯的变化规律及其对荔枝果实失重和商品率的影响。     

黑颈鹤与灰鹤羽毛形态结构特征初步分析

采用显微成像术及形态计量分析技术对黑颈鹤与灰鹤的初级飞羽、次级飞羽、尾羽及绒羽的结构进行了形态学分析,应用单因素分析、独立样本t检验对形态结构量化指标进行了统计分析,并建立了特征指标的Fisher判别函数。结果显示：宏观结构层面,2种鹤类羽毛的色泽形状不同,且初级飞羽、次级飞羽内翈羽枝长,尾羽内翈羽枝与羽干的夹角,初级飞羽羽片面积差异显著;微观结构层面,2种鹤类的次级飞羽有钩羽小枝腹齿数及纤毛对数及羽小钩数,飞羽的无钩羽小枝腹齿数,尾羽的有钩羽小枝羽小钩数,绒羽节状羽小枝的节直径以及节间距等结构指标差异显著。通过对黑颈鹤与灰鹤的羽毛宏观、微观的特征结构进行量化分析,构建了以差异指标为依据的Fisher判别函数,使用回代法检验判别方程的正确率为80.2%。综上所述,通过有钩羽小枝羽小钩数、成对纤毛数、腹齿数,无钩羽小枝腹齿数,内翈羽枝与羽干夹角,内翈羽枝长,羽片面积和羽片羽轴长这8个量化指标,可以对未知来源的灰鹤与黑颈鹤的初级飞羽、次级飞羽、尾羽样本完成种间分类,进一步丰富了我国珍稀鹤类物种鉴定方法或途径。     

可拓工程方法在储粮害虫分类识别中的应用研究

利用图像识别储粮害虫过程中，需解决多种害虫的多特征参数和混和度大的分类识别问题。该文提出应用可拓工程方法，构造储粮害虫特征数据的标准物元矩阵与节域物元矩阵,计算待测粮虫与各类害虫的关联度，以关联度的大小对储粮害虫进行分类判别，程序计算结果表明，该方法可行有效。可拓工程方法应用在储粮害虫分类识别上有应用价值。     

Progress in autoantibodies to platelet and idiopathic thrombocytopenic purpura

The onset of idiopathic thrombocytopenic purpura (ITP) is related to excessive destruction of platelet resulting from antiplatelet autoantibody. The immunity with ITP is imbalance, so the hypofunction of suppressor T cell (Ts) can't restrain B lymphocytes producing antibody, as a result, autoant ibody is produced, the clearance of platelet in blood circulation is accelerated. The different antibodies have different functions on ITP. The measuring of platelet antibody has its significance in diagnosis, therapeutic evaluat ion and expectation of prognosis.