全文获取类型
收费全文 | 1122篇 |
免费 | 54篇 |
专业分类
林业 | 42篇 |
农学 | 36篇 |
基础科学 | 21篇 |
159篇 | |
综合类 | 87篇 |
农作物 | 40篇 |
水产渔业 | 73篇 |
畜牧兽医 | 646篇 |
园艺 | 11篇 |
植物保护 | 61篇 |
出版年
2021年 | 9篇 |
2020年 | 12篇 |
2019年 | 25篇 |
2018年 | 13篇 |
2017年 | 14篇 |
2016年 | 20篇 |
2015年 | 19篇 |
2014年 | 26篇 |
2013年 | 54篇 |
2012年 | 30篇 |
2011年 | 50篇 |
2010年 | 25篇 |
2009年 | 32篇 |
2008年 | 58篇 |
2007年 | 62篇 |
2006年 | 39篇 |
2005年 | 45篇 |
2004年 | 40篇 |
2003年 | 33篇 |
2002年 | 41篇 |
2001年 | 30篇 |
2000年 | 30篇 |
1999年 | 33篇 |
1998年 | 13篇 |
1997年 | 14篇 |
1995年 | 14篇 |
1994年 | 11篇 |
1993年 | 12篇 |
1992年 | 22篇 |
1991年 | 17篇 |
1990年 | 13篇 |
1989年 | 19篇 |
1988年 | 23篇 |
1987年 | 13篇 |
1986年 | 15篇 |
1985年 | 22篇 |
1984年 | 15篇 |
1983年 | 14篇 |
1982年 | 11篇 |
1981年 | 8篇 |
1979年 | 16篇 |
1978年 | 10篇 |
1977年 | 9篇 |
1975年 | 16篇 |
1974年 | 14篇 |
1973年 | 11篇 |
1972年 | 8篇 |
1971年 | 12篇 |
1969年 | 11篇 |
1967年 | 8篇 |
排序方式: 共有1176条查询结果,搜索用时 15 毫秒
51.
Intravenous sufentanil‐midazolam versus sevoflurane anaesthesia in medetomidine pre‐medicated Himalayan rabbits undergoing ovariohysterectomy
下载免费PDF全文
![点击此处可从《Veterinary anaesthesia and analgesia》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Patricia Hedenqvist Marianne Jensen‐Waern Åsa Fahlman Ragnvi Hagman Anna Edner 《Veterinary anaesthesia and analgesia》2015,42(4):377-385
ObjectiveTo compare physiological effects of sufentanil-midazolam with sevoflurane for surgical anaesthesia in medetomidine premedicated rabbits.Study designProspective, randomized controlled experimental study.AnimalsEighteen female Himalayan rabbits, weight 2.1 ± 0.1 kg.MethodsPremedication with 0.1 mg kg−1 medetomidine and 5 mg kg−1 carprofen subcutaneously, was followed by intravenous anaesthetic induction with sufentanil (2.3 μg mL−1) and midazolam (0.45 mg mL−1). After endotracheal intubation, anaesthesia was maintained with sufentanil-midazolam (n = 9) or sevoflurane (n = 9). Ovariohysterectomy was performed. Intermittent positive pressure ventilation was performed as required. Physiological variables were studied perioperatively. Group means of physiologic data were generated for different anaesthetic periods. Data were compared for changes from sedation, and between groups by anova. Post-operatively, 0.05 mg kg−1 buprenorphine was administered once and 5 mg kg−1 carprofen once daily for 2–3 days. Rabbits were examined and weighed daily until one week after surgery.ResultsSmooth induction of anaesthesia was achieved within 5 minutes. Sufentanil and midazolam doses were 0.5 μg kg−1 and 0.1 mg kg−1, during induction and 3.9 μg kg−1 hour−1 and 0.8 mg kg−1 hour−1 during surgery, respectively. End-tidal sevoflurane concentration was 2.1% during surgery. Assisted ventilation was required in nine rabbits receiving sufentanil-midazolam and four receiving sevoflurane. There were no differences between groups in physiologic data other than arterial carbon dioxide. In rabbits receiving sevoflurane, mean arterial pressure decreased pre-surgical intervention, heart rate increased 25% during and after surgery and body weight decreased 4% post-operatively. Post-operative problems sometimes resulted from catheterization of the ear artery.ConclusionSevoflurane and sufentanil-midazolam provided surgical anaesthesia of similar quality. Arterial blood pressure was sustained during sufentanil-midazolam anaesthesia and rabbits receiving sevoflurane lost body weight following ovariohysterectomy. Mechanical ventilation was required with both anaesthetic regimens.Clinical relevanceAnaesthesia with sufentanil-midazolam in medetomidine premedicated healthy rabbits is useful in the clinical and the research setting, as an alternative to sevoflurane. 相似文献
52.
Assessing the influence of mechanical ventilation on blood gases and blood pressure in rattlesnakes
下载免费PDF全文
![点击此处可从《Veterinary anaesthesia and analgesia》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Mads F Bertelsen Rasmus Buchanan Heidi M Jensen Cleo AC Leite Augusto S Abe Søren S Nielsen Tobias Wang 《Veterinary anaesthesia and analgesia》2015,42(4):386-393
ObjectiveTo characterize the impact of mechanical positive pressure ventilation on heart rate (HR), arterial blood pressure, blood gases, lactate, glucose, sodium, potassium and calcium concentrations in rattlesnakes during anesthesia and the subsequent recovery period.Study designProspective, randomized trial.AnimalsTwenty one fasted adult South American rattlesnakes (Crotalus durissus terrificus).MethodsSnakes were anesthetized with propofol (15 mg kg−1) intravenously, endotracheally intubated and assigned to one of four ventilation regimens: Spontaneous ventilation, or mechanical ventilation at a tidal volume of 30 mL kg−1 at 1 breath every 90 seconds, 5 breaths minute−1, or 15 breaths minute−1. Arterial blood was collected from indwelling catheters at 30, 40, and 60 minutes and 2, 6, and 24 hours following induction of anesthesia and analyzed for pH, PaO2, PaCO2, and selected variables. Mean arterial blood pressure (MAP) and HR were recorded at 30, 40, 60 minutes and 24 hours.ResultsSpontaneous ventilation and 1 breath every 90 seconds resulted in a mild hypercapnia (PaCO2 22.4 ± 4.3 mmHg [3.0 ± 0.6 kPa] and 24.5 ± 1.6 mmHg [3.3 ± 0.2 kPa], respectively), 5 breaths minute−1 resulted in normocapnia (14.2 ± 2.7 mmHg [1.9 ± 0.4 kPa]), while 15 breaths minute−1 caused marked hypocapnia (8.2 ± 2.5 mmHg [1.1 ± 0.3 kPa]). Following recovery, blood gases of the four groups were similar from 2 hours. Anesthesia, independent of ventilation was associated with significantly elevated glucose, lactate and potassium concentrations compared to values at 24 hours (p < 0.0001). MAP increased significantly with increasing ventilation frequency (p < 0.001). HR did not vary among regimens.Conclusions and clinical relevanceMechanical ventilation had a profound impact on blood gases and blood pressure. The results support the use of mechanical ventilation with a frequency of 1–2 breaths minute−1 at a tidal volume of 30 mL kg−1 during anesthesia in fasted snakes. 相似文献
53.
Young JF Duthie SJ Milne L Christensen LP Duthie GG Bestwick CS 《Journal of agricultural and food chemistry》2007,55(3):618-623
The polyacetylene falcarinol, isolated from carrots, has been shown to be protective against chemically induced colon cancer development in rats, but the mechanisms are not fully understood. In this study CaCo-2 cells were exposed to falcarinol (0.5-100 microM) and the effects on proliferation, DNA damage, and apoptosis investigated. Low-dose falcarinol exposure (0.5-10 microM) decreased expression of the apoptosis indicator caspase-3 concomitantly with decreased basal DNA strand breakage. Cell proliferation was increased (1-10 microM), whereas cellular attachment was unaffected by <10 microM falcarinol. At concentrations above 20 microM falcarinol, proliferation of CaCo-2 cells decreased and the number of cells expressing active caspase-3 increased simultaneously with increased cell detachment. Furthermore, DNA single-strand breakage was significantly increased at concentrations above 10 microM falcarinol. Thus, the effects of falcarinol on CaCo-2 cells appear to be biphasic, inducing pro-proliferative and apoptotic characteristics at low and high concentrations of falcarinol, respectively. 相似文献
54.
Poulsen KA Young JF Theil P Kolko M Oksbjerg N Lambert IH 《Journal of agricultural and food chemistry》2007,55(5):1970-1976
The role of phospholipase A2 in the induction of drip loss from pig muscle has been investigated. In samples from porcine M. longissimus dorsi, total PLA2 activity as well as mRNA and protein levels of the group VIA iPLA2 (iPLA2-VIA) increased during the initial 4 h post-mortem period. Morphological studies of porcine muscle showed that at 4 h post-mortem, gaps had formed between muscle fibers and that the sarcolemma membrane borders appeared blurred. At the same time iPLA2-VIA protein levels were increased inside muscle fibers and at the sarcolemma. iPLA2-VIA mRNA abundance in samples from different breeds of pigs with variations in drip loss revealed no clear correlation between drip loss level and iPLA2-VIA expression. Together, these data indicate that during the post-mortem period, iPLA2-VIA expression and activity is increased at the muscle fiber membranes. PLA2 activity may affect membrane permeability and consequently the progression of drip formation in porcine muscle. 相似文献
55.
Loader JI Hawkes AD Beuzenberg V Jensen DJ Cooney JM Wilkins AL Fitzgerald JM Briggs LR Miles CO 《Journal of agricultural and food chemistry》2007,55(26):11093-11100
Yessotoxins from a large-scale culture (226 L) of Protoceratium reticulatum strain CAWD129 were harvested by filtration followed by solid-phase extraction. The extract was purified by column chromatography over basic alumina and reverse-phase flash chromatography to afford pure yessotoxin (193 mg). Isolation of yessotoxin was greatly facilitated by selection of a strain which did not produce analogues that interfered with yessotoxin isolation. In addition to yessotoxin, numerous minor yessotoxins were detected by LC-MS in other fractions. From one of these, an early eluting minor analogue with the same molecular weight as yessotoxin and a similar mass spectrometric fragmentation pattern was isolated. This analogue was identified by NMR and mass spectrometry as a novel yessotoxin analogue containing a furan ring in the side chain. This finding reveals biosynthetic flexibility of the yessotoxin pathway in P. reticulatum and confirms earlier findings of production of many minor yessotoxin analogues by this alga. Production of these analogues appeared to be a constitutive trait of P. reticulatum CAWD129. 相似文献
56.
Caroline Larsson Anne Vitger Rasmus B Jensen Peter Junghans Anne-Helene Tauson 《Acta veterinaria Scandinavica》2014,56(1)
Background
Overweight and obesity are the most common nutritional disorders in dogs and may lead to various secondary diseases and decreased lifespan. In obesity research, measurement of energy expenditure (EE) and determination of the energy requirements are essential. The objective with this study was to validate and evaluate the suitability of the oral 13C-bicarbonate technique (o13CBT) for measuring EE in dog obesity studies. A further objective was to investigate the impact of body weight (BW) reduction and changes in body composition on the EE when measured under conditions corresponding to the basal metabolic rate (BMR).Results
The EE in five privately owned, overweight dogs was measured simultaneously with the o13CBT and indirect calorimetry (IC) for comparison of the results. Two measurements per dog were performed under the same standardised conditions (i.e. fasted and resting state) at the start, and after completing a 12-week BW reduction program. Additionally, measurements of body composition by Dual-energy X-ray absorptiometry (DEXA) were conducted at the beginning and at the end of the BW reduction program. There were no differences in EE results obtained by the o13CBT and IC. Overweight and the BW reduction did not affect the estimates for the respiratory quotient (RQ) or the recovery factor for the 13C-tracer (RF), both needed when using the o13CBT. The dogs lost 16% (SD ± 2.0) of their initial BW in reduced fat mass (P < 0.001), whereas fat free mass (FFM) remained unchanged. There was no effect of the BW reduction on the determined EE expressed in kJ/kg BW/d, or in kJ/kg BW0.75/d. However, EE was lower (P < 0.001) after the BW reduction program when expressed in relation to FFM (kJ/kg FFM/d).Conclusions
Results from the present study show that the o13CBT can be a used in obesity research to determine EE in fasted dogs and under resting conditions. Furthermore, the results suggest that the BMR does not change with reduced BW in overweight dogs as long as the FFM remains unchanged. This indicates that the BMR to maintain one gram of fat is equal to maintaining one gram of FFM in overweight dogs. 相似文献57.
Søren Saxmose Nielsen Anne Marie Michelsen Henrik Elvang Jensen Kristiane Barington Katharina Vester Opstrup Jens Frederik Agger 《Preventive veterinary medicine》2014
Skin lesions on pigs inflicted by humans compromise animal welfare and are the subject of increased public and political attention in Denmark. Systematic surveillance of such skin lesions was enforced in April 2010 at all Danish pig abattoirs, through the recording of meat inspection Code 904 for the presence of skin lesions suspected to be human inflicted. The objectives of the present study were to (a) estimate the apparent prevalence of Code 904s at the pig and herd owner level; (b) characterise the distribution of deliveries with pigs demonstrating a Code 904; (c) characterise the distribution of herd owners with repeated Code 904 recordings; and (d) determine the developments in Code 904 prevalence over time in Danish finishing pigs in the period from May 1, 2010 to September 30, 2013. 相似文献
58.
Ken Steen Pedersen Markku Johansen ?ystein Angen Sven Erik Jorsal Jens Peter Nielsen Tim K Jensen Roberto Guedes Marie St?hl Poul B?kbo 《Irish veterinary journal》2014,67(1)
Background
The major indication for antibiotic use in Danish pigs is treatment of intestinal diseases post weaning. Clinical decisions on antibiotic batch medication are often based on inspection of diarrhoeic pools on the pen floor. In some of these treated diarrhoea outbreaks, intestinal pathogens can only be demonstrated in a small number of pigs within the treated group (low pathogen diarrhoea). Termination of antibiotic batch medication in herds suffering from such diarrhoea could potentially reduce the consumption of antibiotics in the pig industry. The objective of the present pilot study was to suggest criteria for herd diagnosis of low pathogen diarrhoea in growing pigs.Data previously collected from 20 Danish herds were used to create a case series of clinical diarrhoea outbreaks normally subjected to antibiotic treatment. In the present study, these diarrhoea outbreaks were classified as low pathogen (<15% of the pigs having bacterial intestinal disease) (n =5 outbreaks) or high pathogen (≥15% of the pigs having bacterial intestinal disease) (n =15 outbreaks). Based on the case series, different diagnostic procedures were explored, and criteria for herd diagnosis of low pathogen diarrhoea were suggested. The effect of sampling variation was explored by simulation.Results
The diagnostic procedure with the highest combined herd-level sensitivity and specificity was qPCR testing of a pooled sample containing 20 randomly selected faecal samples. The criteria for a positive test result (high pathogen diarrhoea outbreak) were an average of 1.5 diarrhoeic faecal pools on the floor of each pen in the room under investigation and a pathogenic bacterial load ≥35,000 per gram in the faecal pool tested by qPCR. The bacterial load was the sum of Lawsonia intracellularis, Brachyspira pilosicoli and Escherichia coli F4 and F18 bacteria per gram faeces. The herd-diagnostic performance was (herd-level) diagnostic sensitivity =0.99, diagnostic specificity =0.80, positive predictive value =0.94 and negative predictive value =0.96.Conclusions
The pilot study suggests criteria for herd diagnosis of low pathogen diarrhoea in growing pigs. The suggested criteria should now be evaluated, and the effect of terminating antibiotic batch medication in herds identified as suffering from low pathogen diarrhoea should be explored.Electronic supplementary material
The online version of this article (doi:10.1186/2046-0481-67-24) contains supplementary material, which is available to authorized users. 相似文献59.
Rasoul Yousefpour Jette Bredahl Jacobsen Henrik Meilby Bo Jellesmark Thorsen 《Annals of Forest Science》2014,71(2):301-312
Context
We develop a modelling concept that updates knowledge and beliefs about future climate changes, to model a decision-maker’s choice of forest management alternatives, the outcomes of which depend on the climate condition.Aims
Applying Bayes’ updating, we show that while the true climate trajectory is initially unknown, it will eventually be revealed as novel information become available. How fast the decision-maker will form firm beliefs about future climate depends on the divergence among climate trajectories, the long-term speed of change, and the short-term climate variability.Methods
We simplify climate change outcomes to three possible trajectories of low, medium and high changes. We solve a hypothetical decision-making problem of tree species choice aiming at maximising the land expectation value (LEV) and based on the updated beliefs at each time step.Results
The economic value of an adaptive approach would be positive and higher than a non-adaptive approach if a large change in climate state occurs and may influence forest decisions.Conclusion
Updating knowledge to handle climate change uncertainty is a valuable addition to the study of adaptive forest management in general and the analysis of forest decision-making, in particular for irreversible or costly decisions of long-term impact. 相似文献60.
A spawning and egg collecting system for fish with pelagic eggs is described and discussed. The spawning unit is a 175 m3 submersible plastic pen supported by a polyethylene floating collar. The eggs are collected by rotating the water column in the pen and placing a net in the rotating water. The system is inexpensive and easy to operate. Temperature and illumination control are feasible.The quantity collected in the 1981 spawning season was 138 million eggs. Obtained eggs were of good quality. The theoretical spawning potential of the brood stock was 271 million eggs. This difference was mainly ascribed to suboptimal collection, but also to incomplete spawning and sinking of unfertilized and dead eggs. 相似文献