全文获取类型
收费全文 | 322篇 |
免费 | 18篇 |
专业分类
林业 | 41篇 |
农学 | 7篇 |
64篇 | |
综合类 | 27篇 |
农作物 | 9篇 |
水产渔业 | 14篇 |
畜牧兽医 | 131篇 |
园艺 | 18篇 |
植物保护 | 29篇 |
出版年
2023年 | 1篇 |
2022年 | 1篇 |
2021年 | 3篇 |
2020年 | 9篇 |
2019年 | 4篇 |
2018年 | 4篇 |
2017年 | 6篇 |
2016年 | 10篇 |
2015年 | 5篇 |
2014年 | 9篇 |
2013年 | 10篇 |
2012年 | 18篇 |
2011年 | 30篇 |
2010年 | 12篇 |
2009年 | 11篇 |
2008年 | 25篇 |
2007年 | 19篇 |
2006年 | 27篇 |
2005年 | 22篇 |
2004年 | 21篇 |
2003年 | 22篇 |
2002年 | 22篇 |
2001年 | 6篇 |
2000年 | 4篇 |
1999年 | 3篇 |
1998年 | 6篇 |
1997年 | 4篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1990年 | 5篇 |
1989年 | 1篇 |
1987年 | 1篇 |
1985年 | 2篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1971年 | 1篇 |
1968年 | 1篇 |
1954年 | 2篇 |
1953年 | 1篇 |
排序方式: 共有340条查询结果,搜索用时 31 毫秒
91.
Pascal Lecomte Jean-Pierre Paulin Eve Billing 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(7):667-675
The value of BIS for blossom blight risk assessment was studied from data collected in an experimental orchard in south-west France. Trees observed included mature commercial pear and apple trees and some young trees in experimental plots. There was a weather station in the orchard and beehives were present. Field records included flowering times of the pear and apple cultivars studied (mostly Passe-Crassane and Beurré Hardy, Royal Gala and Golden Delicious) and dates when blossom blight was first seen on each cultivar. Between 1980 and 1991, records of blight were available for 25 cases. In most cases, one or more infection risk (IR) days, as defined for BIS, could be found during bloom. DD13 mean sums (sums of degree days above a mean temperature of 13 °C) gave good guidance on times when early signs of blossom blight were present in 14 cases. There was only a slight divergence from BIS guides in a further five cases. Possible reasons for divergence and for non-fit in the remaining six cases are discussed. It is concluded from this study that BIS should give useful guidance on optimal times for protective spray applications and for timing of searches for signs of early blossom blight in south-west of France. Graphical presentations of data provide additional information. 相似文献
92.
93.
Boyen F Pasmans F Van Immerseel F Morgan E Botteldoorn N Heyndrickx M Volf J Favoreel H Hernalsteens JP Ducatelle R Haesebrouck F 《Veterinary microbiology》2008,128(3-4):364-373
Virulence genes regulated by the SsrA/B system are indispensable for systemic disease in BALB/c mice. The role of this regulating system in the pathogenesis of Salmonella Typhimurium infections in pigs is not documented. In the present study, the interactions of Salmonella Typhimurium and an ssrA/B mutant were compared in vitro and in vivo. The ssrA/B mutant strain displayed decreased Salmonella Pathogenicity Island 2 (SPI-2) expression levels, showed a replication defect in mouse macrophages and was attenuated in a mouse model after oral inoculation. Using real time qRT-PCR and a porcine ileal loop model, it was shown that the ssrA/B mutant strain was not significantly attenuated in overall virulence and SPI-1 expression in specific. Flowcytometric analysis demonstrated that the ssrA/B mutant strain was defective in intracellular replication in porcine macrophages. After oral inoculation of piglets with the wild type strain or the ssrA/B mutant strain, the animals of both groups excreted Salmonella and were colonized by Salmonella to the same extent. In an intravenous mixed infection model, the ssrA/B mutant strain was defective in the colonization of several internal organs. These results suggest that the ssrA/B gene of Salmonella Typhimurium plays a limited role in the persistent intestinal colonization of pigs. 相似文献
94.
Scale-dependent determinants of heterogeneity in fire frequency in a coniferous boreal forest of eastern Canada 总被引:1,自引:0,他引:1
Despite the recognized importance of fire in North American boreal forests, the relative importance of stochastic and determinist
portions of intra-regional spatial variability in fire frequency is still poorly understood. The first objective of this study
is to identify sources of spatial variability in fire frequency in a landscape of eastern Quebec’s coniferous boreal forest.
Broad-scale environmental factors considered included latitude, longitude, human activities and belonging to a given bioclimatic
domain, whereas fine-scale factors included slope, position on the slope, aspect, elevation, surficial deposit and drainage.
The average distance to waterbodies was also considered as a potential intermediate-scale source of variability in fire frequency.
In order to assess these environmental factors’ potential influence, they were incorporated into a proportional hazard model,
a semi-parametric form of survival analysis. We also used a digital elevation model in order to evaluate the dominant aspect
within neighborhoods of varying sizes and successively incorporated these covariates into the proportional hazard model. We
found that longitude significantly affects fire frequency, suggesting a maritime influence on fire frequency in this coastal
landscape. We also found that position on the slope was related to fire frequency since hilltops and upperslopes were subject
to a lower fire frequency. Dominant aspect was also related to fire frequency, but only when characterized within a neighborhood
delimited by 4,000 to 10,000-m radii (5,027–31,416 ha). A 2–6-fold variation in fire frequency can be induced by geographic
and topographic contexts, suggesting a substantial intra-regional heterogeneity in disturbance regime with potential consequences
on forest dynamics and biodiversity patterns. Implications for forest management are also briefly discussed.
Electronic supplementary material The online version of this article (doi: ) contains supplementary material, which is available to authorized users. 相似文献
95.
Cordeau ME Joubert P Dewachi O Hamid Q Lavoie JP 《Veterinary immunology and immunopathology》2004,97(1-2):87-96
Heaves is a common condition of horses of cold climate that is characterized by small airway inflammation and obstruction following exposure of susceptible horses to moldy hay and straw. It has been shown that helper T lymphocytes (Th) orchestrate the inflammatory response in asthma and in various animal models of allergic lung diseases by the release of Th2-type cytokines. Results of previous studies indicate that a predominant expression of Th2-type response by airway cells may also be present in heaves. To evaluate the temporal mRNA expression of Th1 (IFN-gamma) and Th2 (IL-4, IL-5) type cytokines in heaves and their relationship to clinical disease, we studied the pulmonary mechanics and cytokine mRNA expression (IL-4, IL-5 and IFN-gamma) in bronchoalveolar lavage lymphocytes of horses with heaves (n=6) and control (n=6) before and after 24h and 9 days of continuous natural inhalation challenge. Starting 24h after challenge horses with heaves, but not control horses, had a significant increase in pulmonary elastance and the number of lymphocytes expressing mRNA for IL-4 and IL-5. These changes were further increased at 9 days, at which time the number of cells positive for IFN-gamma mRNA was decreased. In this study we have shown that BAL lymphocytes of horses with heaves during clinical exacerbation have a predominant Th2-type cytokine response and that this response coincides in time with the presence of airway obstruction. 相似文献
96.
97.
Acibenzolar-S-methyl Induces the Accumulation of Defense-related Enzymes in Apple and Protects from Fire Blight 总被引:4,自引:0,他引:4
Marie-Noëlle Brisset Sophie Cesbron Sherman V. Thomson Jean-Pierre Paulin 《European journal of plant pathology / European Foundation for Plant Pathology》2000,106(6):529-536
Acibenzolar-S-methyl (Novartis) is a chemical inducer of systemic acquired resistance in several annual plants. The ability of this novel chemical to induce resistance was studied in a perennial plant (apple) affected by fire blight caused by Erwinia amylovora. Acibenzolar-S-methyl (100 and 200mg/l active ingredient) protected Golden Delicious seedlings, scions and trees from artificial infection when applied before inoculation. The protection of apple seedlings was similar to the protection obtained with the standard for fire blight control, plantomycin (100mg/l streptomycin sulfate), applied immediately before inoculation. The mean levels of control in scions in the greenhouse and in trees in orchards were approximately 69% and 50%, respectively. The protection of apple seedlings was constantly associated with the activation of two families of defense-related enzymes, peroxidases and -1,3-glucanases. Accumulation of both enzymes was induced locally in treated leaves and systemically, especially for -1,3-glucanases, in upper untreated leaves, and was sustained for at least 17 days. These results suggest that acibenzolar-S-methyl promotes induced systemic resistance in apple by increasing defense-related compounds. This chemical could provide a new approach of control of fire blight but its practical use needs further investigation. 相似文献
98.
Jean-Paul Laclau Jacques Ranger José Leonardo de Moraes Gonçalves Valérie Maquère Alex V. Krusche Armel Thongo M’Bou Yann Nouvellon Laurent Saint-André Jean-Pierre Bouillet Marisa de Cassia Piccolo Philippe Deleporte 《Forest Ecology and Management》2010,259(9):1771-1785
The sustainability of fast-growing tropical Eucalyptus plantations is of concern in a context of rising fertilizer costs, since large amounts of nutrients are removed with biomass every 6–7 years from highly weathered soils. A better understanding of the dynamics of tree requirements is required to match fertilization regimes to the availability of each nutrient in the soil. The nutrition of Eucalyptus plantations has been intensively investigated and many studies have focused on specific fluxes in the biogeochemical cycles of nutrients. However, studies dealing with complete cycles are scarce for the Tropics. The objective of this paper was to compare these cycles for Eucalyptus plantations in Congo and Brazil, with contrasting climates, soil properties, and management practices.The main features were similar in the two situations. Most nutrient fluxes were driven by crown establishment the two first years after planting and total biomass production thereafter. These forests were characterized by huge nutrient requirements: 155, 10, 52, 55 and 23 kg ha?1 of N, P, K, Ca and Mg the first year after planting at the Brazilian study site, respectively. High growth rates the first months after planting were essential to take advantage of the large amounts of nutrients released into the soil solutions by organic matter mineralization after harvesting. This study highlighted the predominant role of biological and biochemical cycles over the geochemical cycle of nutrients in tropical Eucalyptus plantations and indicated the prime importance of carefully managing organic matter in these soils. Limited nutrient losses through deep drainage after clear-cutting in the sandy soils of the two study sites showed the remarkable efficiency of Eucalyptus trees in keeping limited nutrient pools within the ecosystem, even after major disturbances. Nutrient input–output budgets suggested that Eucalyptus plantations take advantage of soil fertility inherited from previous land uses and that long-term sustainability will require an increase in the inputs of certain nutrients. 相似文献
99.
Benjamin Dumont Vincent Leemans Salvador Ferrandis Bernard Bodson Jean-Pierre Destain Marie-France Destain 《Precision Agriculture》2014,15(3):255-272
The real-time non-invasive determination of crop biomass and yield prediction is one of the major challenges in agriculture. An interesting approach lies in using process-based crop yield models in combination with real-time monitoring of the input climatic data of these models, but unknown future weather remains the main obstacle to reliable yield prediction. Since accurate weather forecasts can be made only a short time in advance, much information can be derived from analyzing past weather data. This paper presents a methodology that addresses the problem of unknown future weather by using a daily mean climatic database, based exclusively on available past measurements. It involves building climate matrix ensembles, combining different time ranges of projected mean climate data and real measured weather data originating from the historical database or from real-time measurements performed in the field. Used as an input for the STICS crop model, the datasets thus computed were used to perform statistical within-season biomass and yield prediction. This work demonstrated that a reliable predictive delay of 3–4 weeks could be obtained. In combination with a local micrometeorological station that monitors climate data in real-time, the approach also enabled us to (i) predict potential yield at the local level, (ii) detect stress occurrence and (iii) quantify yield loss (or gain) drawing on real monitored climatic conditions of the previous few days. 相似文献
100.
Bishop JL Dobrea EZ McKeown NK Parente M Ehlmann BL Michalski JR Milliken RE Poulet F Swayze GA Mustard JF Murchie SL Bibring JP 《Science (New York, N.Y.)》2008,321(5890):830-833
Observations by the Mars Reconnaissance Orbiter/Compact Reconnaissance Imaging Spectrometer for Mars in the Mawrth Vallis region show several phyllosilicate species, indicating a wide range of past aqueous activity. Iron/magnesium (Fe/Mg)-smectite is observed in light-toned outcrops that probably formed via aqueous alteration of basalt of the ancient cratered terrain. This unit is overlain by rocks rich in hydrated silica, montmorillonite, and kaolinite that may have formed via subsequent leaching of Fe and Mg through extended aqueous events or a change in aqueous chemistry. A spectral feature attributed to an Fe2+ phase is present in many locations in the Mawrth Vallis region at the transition from Fe/Mg-smectite to aluminum/silicon (Al/Si)-rich units. Fe2+-bearing materials in terrestrial sediments are typically associated with microorganisms or changes in pH or cations and could be explained here by hydrothermal activity. The stratigraphy of Fe/Mg-smectite overlain by a ferrous phase, hydrated silica, and then Al-phyllosilicates implies a complex aqueous history. 相似文献