全文获取类型
收费全文 | 78篇 |
免费 | 3篇 |
专业分类
林业 | 1篇 |
农学 | 1篇 |
7篇 | |
综合类 | 1篇 |
农作物 | 2篇 |
水产渔业 | 10篇 |
畜牧兽医 | 48篇 |
植物保护 | 11篇 |
出版年
2021年 | 5篇 |
2020年 | 6篇 |
2019年 | 6篇 |
2018年 | 3篇 |
2017年 | 4篇 |
2016年 | 2篇 |
2015年 | 3篇 |
2014年 | 3篇 |
2013年 | 6篇 |
2012年 | 8篇 |
2011年 | 3篇 |
2010年 | 1篇 |
2009年 | 1篇 |
2008年 | 7篇 |
2007年 | 1篇 |
2006年 | 3篇 |
2005年 | 2篇 |
2003年 | 3篇 |
2002年 | 3篇 |
2001年 | 2篇 |
2000年 | 3篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1983年 | 1篇 |
1981年 | 1篇 |
排序方式: 共有81条查询结果,搜索用时 31 毫秒
11.
Jaqueline Mena Carolina Manosalva Ruben Ramirez Lhia Chandia Daniel Carroza Anitsi Loaiza Rafael A. Burgos Maria A. Hidalgo 《Veterinary immunology and immunopathology》2013,151(3-4):275-284
Neutrophils are critical to the innate immune response; therefore, the proper function of neutrophils is critical to avoid the development of certain diseases. Linoleic acid, a polyunsaturated long-chain fatty acid, is one of the most abundant long-chain fatty acids found in the plasma of cows after giving birth. In this study, we evaluated the effects of linoleic acid treatment on bovine neutrophil adhesion, chemotaxis, metalloproteinase (MMP)-9 release, CD11b expression, intracellular calcium mobilisation, mitogen-activating protein kinase (MAPK) phosphorylation and COX-2 and IL-8 expression. Bovine neutrophils isolated from healthy heifers were incubated with different concentrations of linoleic acid, and then neutrophil responses were evaluated. Our results show that the treatment of neutrophils with 100 μM linoleic acid increased their adhesion to the bovine endothelial cell line CPA47. The results of a transwell migration assay revealed that linoleic acid could also promote the chemotaxis of bovine neutrophils. Furthermore, linoleic acid treatment increased MMP-9 activity and CD11b cell surface expression in neutrophils. Fifty and 100 μM linoleic acid also increased intracellular calcium mobilisation in neutrophils loaded with Fluo-4 AM dye. Linoleic acid also rapidly (2–5 min) stimulated the phosphorylation of ERK1/2 and p38 MAPK as evaluated by immunoblot. Finally, COX-2 and IL-8 mRNA expression increased after 2 h of linoleic acid treatment. In conclusion, linoleic acid stimulates adhesion, chemotaxis, granule release and intracellular responses in bovine neutrophils. 相似文献
12.
Jaqueline Magalhães Pereira Edson Luiz Lopes Baldin Everton Pires Soliman Carlos Frederico Wilcken 《Phytoparasitica》2013,41(2):117-124
The red gum lerp psyllid Glycaspis brimblecombei Moore (Hemiptera: Psyllidae) was first recorded in Brazil in 2003 and since then has caused serious damage in Eucalyptus forests. The damage is mainly due to defoliation, sooty mold and dieback, thus requiring the development of efficient management strategies. The settling and ovipositional preference of G. brimblecombei by species and clones of Eucalyptus were evaluated in free- and no-choice tests. Preliminary tests were done to determine the density of psyllid couples that produced the greatest densities of eggs, leaf position selection for oviposition, and whether oviposition was affected by preimaginal conditioning. Apical leaves were used more frequently for oviposition by the psyllid, and the number of eggs decreased from the apex to the base. The densities of 10 and 15 psyllid couples per seedling produced the highest oviposition. There was no evidence of preimaginal conditioning. In the free-choice test, E. grandis, E. urophylla, VM-1, I-144, C-219 and H-13 were less attractive to G. brimblecombei adults, with H-13, E. grandis and E. urophylla having the least oviposition. In the no-choice test, E. urophylla, GG-100 and E. grandis also demonstrated a lower oviposition, but E. camaldulensis and 3025 were highly susceptible. The low preference for the genotypes E. grandis and E. urophylla suggests the occurrence of a non-preference type resistance against the red gum lerp psyllid. Our results can be utilized for the development of management programs for G. brimblecombei in Eucalyptus forests. 相似文献
13.
Ribeiro VL dos Santos JC Martins JR Schripsema J Siqueira IR von Poser GL Apel MA 《Veterinary parasitology》2011,179(1-3):195-198
Calea serrata Less. (Asteraceae), an endemic species of south Brazil known as "quebra-tudo", is used in Afro-Brazilian religious rituals and in folk medicine for treating liver disorders. Phytochemical studies of the n-hexane extract of this plant demonstrated the presence of precocene II, a benzopyran derivative known for its insecticidal activity. The aim of this work was to isolate this benzopyran and determine the chemical composition of the essential oil of C. serrata and further to evaluate the acaricidal activity of the essential oil and precocene II against the larvae of Rhipicephalus (Boophilus) microplus. The LC(99.9) and LC(50) values obtained with the oil, which presents precocene II and sesquiterpenes, were 3.94 μL/mL and 0.28 μL/mL, respectively. For precocene II this values were 4.25mg/mL and 1.78 mg/mL, respectively. The results indicate a synergistic interaction between the components of the oil and precocene II. 相似文献
14.
The objective of the present study was to determine the efficiency of blood neutrophils (PMN) taken from sheep during acute stress. Ten healthy Charolle sheep were sampled before treatment (T0) and 1 (T1), 2 (T2), 24 (T24) and 48 (T48) hours after 1-24ACTH administration. Ten sheep serving as the controls were sampled at the same time intervals, using saline solution instead of 1-24ACTH. At each time sampling, rectal temperature, heart rate, cortisol, glucose, non-esterified fatty acids (NEFA), total and differential leukocyte counts were evaluated. PMN were isolated after centrifugation of whole blood and hypotonic lysis of RBC. Chemotaxis was evaluated on a modified Boyden chamber using a nitrate cellulose filter and both Zymosan activated serum (ZAS) and interleukin-8 (IL-8) as chemoattractants. Phagocytosis was measured using both non-opsonized latex beads and fluoresceinated yeasts opsonized with homologous serum. Superoxide (O(-)2) production was evaluated by measuring superoxide dismutase-inhibitable reduction of ferricytochrome C, and adherence by a colorimetric assay of acid phosphatase activity of adherent cells. The administration of 1-24ACTH induced an acute stress reaction, indicated by the presence of clinical, biochemical and hematological changes. Adherence significantly increased from T0 to T2 in treated sheep. This might be responsible for the depression of non-specific immunity in stressed animals. Studies using stressors other than 1-24 ACTH are needed to verify the influence of other components of the stress reaction on PMN functions. 相似文献
15.
The influence of the obstetrical condition on canine neonatal pulmonary functional competence 下载免费PDF全文
16.
Comazzi S Gelain ME Spagnolo V Riondato F Guglielmino R Sartorelli P 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2006,35(1):47-54
BACKGROUND: In dogs, flow cytometry is used in the phenotyping of immunologic cells and in the diagnosis of hemic neoplasia. However, the paucity of specific antibodies for myeloid cells and B lymphocytes and of labeled antibodies for multicolor techniques limits the ability to detect all leukocyte subpopulations. This is especially true for neoplastic and precursor cells. CD18 and CD45 are expressed on all leukocytes and are involved in cell activation, and together could be useful in helping determine cell lineage. OBJECTIVES: The purpose of this study was to double label canine blood for CD18 and CD45 and to use the differential expression of antigens to identify leukocyte populations in dogs with non-neoplastic and neoplastic hematologic diseases. METHODS: A template was developed using blood samples from 10 clinically healthy dogs and a back-gating technique. Differential leukocyte counts obtained with the template were compared with those obtained by manual and automated methods on blood samples from 17 additional healthy dogs. Blood samples obtained from 9 dogs with non-neoplastic (reactive) hematologic diseases and 27 dogs with hemic neoplasia were double stained for CD18 and CD45 using mouse anticanine CD18 monoclonal antibody (mAb) plus phycoerythrin-conjugated rat anticanine CD45 mAb and fluorescein isothiocyanate-conjugated rabbit antimouse IgG. Hemic neoplasms were diagnosed by cell morphology, and immunophenotypic and cytochemical markers. RESULTS: With the double label, neutrophils, eosinophils, monocytes, and T- and B-lymphocytes were identified. In reactive disorders, a population of activated neutrophils with high CD45 and CD18 expression was detected. In hemic neoplasia, cell lineage was easily determined, even in acute leukemia. CONCLUSIONS: Double labeling for CD18/CD45 may be useful as a screening method to evaluate hematologic diseases and help determine cell lineage, and to aid in the selection of a panel of antibodies that would be useful for further analysis. 相似文献
17.
A Locatelli P Sartorelli F Agnes G P Bondiolotti G B Picotti 《The British veterinary journal》1989,145(6):517-522
The adrenal responses in calves submitted to simulated transport on three occasions for 30 min were evaluated. Plasma adrenaline, cortisol and NEFA increased significantly during simulated transport but became less marked in successive trials. Haematological stress-related parameters (Hb, PCV) increased to the same extent on repeated exposure to simulated transport. Plasma noradrenaline, glucose and cholesterol values were unchanged throughout the study. 相似文献
18.
Jaqueline Matos Cruz Ivo Shodji Tamada Paulo Renato Matos Lopes Renato Nallin Montagnolli Ederio Dino Bidoia 《Water, air, and soil pollution》2014,225(5):1-9
Biogas must be pretreated before its use; thus, both physical and chemical methods have been implemented to remove the fuel’s principal pollutants (CO2 and H2S). Additional removal methods that use microorganisms’ biological processes to eliminate pollutants have also emerged. A selection was made from six bacterial isolates to obtain consortia that removed CO2 and eventually H2S through the enrichment of cultures and the construction of clone libraries of gene 16S ribosomal DNA (rDNA). The results indicate that the principal differences between consortia were determined in the culture medium. C5 and C6 consortia had photosynthetic biomass 1.42 and 1.52 μg/ml, respectively, and concentration of dissolved CO2 100.6 and 99.1 mg/l, respectively. The clone libraries showed that Rhodopseudomonas sp. had percentages 46.6, 42.5, and 86.8 % in C4, C5, and C6, respectively; Xanthobacter sp., 24.5 %, Castellaniella sp., 18 % in C5, and Sphingobium sp., 39.2 % in C4. 相似文献
19.
Clever Briedis João Carlos de Moraes Sá Eduardo Fávero Caires Jaqueline de Fátima Navarro Thiago Massao Inagaki Adriane Boer Caio Quadros Neto Ademir de Oliveira Ferreira Lutécia Beatriz Canalli Josiane Burkner dos Santos 《Geoderma》2012
The stabilisation of soil organic matter (SOM) is the result of the simultaneous action of three mechanisms: chemical stabilisation, biochemical stabilisation and physical protection. The objectives of this study were (i) to evaluate carbon-protection mechanisms in different SOM pools in soil aggregates and (ii) to identify the association of Ca2 + with total organic carbon (TOC) under the influence of surface liming in a medium-textured Oxisol in a long-term experiment under no-till system (NTS) in southern Brazil (25° 10′ S, 50° 05′ W). The treatments consisted of application of zero or 6 tons ha? 1 of dolomitic lime on the soil surface in 1993 and a reapplication of zero or 3 tons ha? 1 of dolomitic lime in 2000 to plots with or without previous lime application. Soil samples collected at depths of 0–2.5, 2.5–5, 5–10 and 10–20 cm were separated into seven aggregate classes. In each of these classes, TOC, particulate organic carbon (POC) and mineral-associated organic carbon (MAOC) were analysed. The 8–19 mm sized aggregates from the 0–2.5 cm layer were assessed by energy-dispersive X-ray spectroscopy (EDS) for the elemental analysis of carbon (C) and calcium (Ca). The liming caused an accumulation of TOC in the aggregates, mainly at a depth of 0–2.5 cm. The aggregates from soils treated with lime had a higher mean weight diameter (MWD) that resulted in the accumulation of TOC, especially in the 8–19 mm aggregate class, that was linear and closely related with C input (R2 = 0.99). The proportion of large aggregates in the treatments with lime was closely correlated with the TOC content of the whole sample. The largest dose of lime (9 tons ha? 1) resulted in higher TOC, POC and MAOC values, mainly in the 8–19 mm aggregate class. The elemental analyses for C and Ca revealed similar spectra between them for the surface-liming treatments in the clay fraction found in the centres of the 8–19 mm aggregates. The surface application of lime to NT fields provided greater stability and protection of the intra-aggregate C, presumably due to Ca2 + acting as a cationic bridge between OC and the kaolinite in the clay fraction. 相似文献
20.
Summary The extent of the weet beet problem in Eastern England is described and the factors to the evolution of weed beet are outlined. Some preliminary experiments designed to distinguish between wild, weed and cultivated populations of beet are described. The response of salt (sodium chloride) solution is a potentially useful discriminating character whereas variation in morphometric characters was not so conclusive. Preliminary results on variation in cytological characters suggest that chiasma frequency is lower in weed than in wild populations and cultivars ofBeta vulgaris. Finally the possible role of crop-related weeds in breeding is discussed.
We are deeply impressed by the untimely death of Dr. A. Evans. The editors. 相似文献
Die Evolution der Unkrautrüben in Zuckerrübenbeständen
Zusammenfassung Es wird die Verbreitung des Problems der Unkrautrüben im östlichen England beschrieben und die Faktoren, die die Evolution der Unkrautrübenpopulation beeinflussen, werden umrissen. Einige vorläufige Versuche zur Unterscheidung zwischen Wild-, Unkraut- und kultivierten Populationen werden beschrieben. Die Reaktion auf Salzlösung (Natriumchlorid) zeigte sich als eine brauchbare Trennmethode, während die Variation in der morphometrischen Charakterisierung nicht so überzeugend war. Vorläufige Ergebnisse über die Unterschiede in der cytologischen Charakterisierung weisen darauf hin, daß die Chiasmafrequenz in Unkrautrüben geringer ist, als in Wildpopulationen und Kultursorten vonBeta vulgaris. Abschließend wird die mögliche Bedeutung der den Kulturpflanzen verwandten Unkräuter für die Züchtung diskutiert.
Beta L. ë
Beta ë , . , , . ( ) , . , ë , Beta vulgaris. , .
We are deeply impressed by the untimely death of Dr. A. Evans. The editors. 相似文献