岷江上游干旱河谷岷江柏木幼林地绿肥种植研究

在岷江上游干旱河谷岷江柏木幼林地经过连续4 a利用雨季种植绿肥压青,增加土壤肥力,提高水分利用率,促进幼树生长研究。结果表明:(1)种植红豆草的土壤0～40 cm含水量旱季增加3.68 g·kg-1,0～20 cm土壤容重降低0.07 g·cm-3;种植草木樨土壤0～40 cm含水量旱季增加4.18 g·kg-1,0～20 cm土壤容重降低0.05 g·cm-3;种植沙打旺土壤0～40 cm含水量增加2.46 g.kg-1,0～20 cm土壤容重降低0.18 g·cm-3。(2)种植绿肥压青,土壤有机质增加0.37 g·kg-1～0.55 g·kg-1,土壤CaCO3含量下降0.66 g·kg-1～1.36 g·kg-1,有效氮增加4.5 mg·kg-1～18.5 mg·kg-1,有效磷含量增加1.0～3.46 mg·kg-1,CEC比对照增加0.54～0.58mg.100g-1。(3)种植红豆草、草木樨、沙打旺绿肥压青对岷江柏木树高、地径生长有显著的促进作用,尤其种植红豆草的岷江柏木幼树生长最好,高、径生长为对照的1.7倍和1.6倍。     

台湾桤木采穗圃建立及扦插繁殖技术研究

经过2a多对台湾桤木采穗圃的建立和管理及扦插试验研究,结果表明：（1）0．5a生优树建立的采穗圃第2a的株采穗量达71条;（2）400mg·L^-1GGR6号生根粉水溶液浸泡的插穗生根率最高,达96．5％,清水处理的穗条效果最差,生根率为67．6％;（3）一年中插穗在6月、9月扦插效果最好,成活率分别可达96．67％、97．35％;（4）插穗摘除1／3叶片扦插效果最好,生根率迭96．3％;（5）母树上部穗条扦插效果最佳,成活率可达97％;（6）台湾桤木插穗以皮部生根为主,属于皮部和愈伤组织混合生根型,夏季扦插60d后成活穗条皮部生根的占83．33％。     

浅谈海南草坪植物的种植设计

草坪是园林造景的重要景观元素之一.本文通过综合论述草坪植物的设计原理,草坪植物空间的营造,及疏林草坪、稀树草坪、开阔草坪、草坪边缘植物景观的种植形式等,针对性地探讨海南热带草坪植物种植设计方法与思路.     

Immunogenicity of bovine parainfluenza type 3 virus proteins encapsulated in nanoparticle vaccines,following intranasal administration to mice

Stimulation of long lasting, protective immunity to respiratory viruses is often difficult to achieve with conventional respiratory vaccines. Polymeric nanoparticles, incorporating viral proteins have been shown to offer sustained release of antigen, with consequent prolongued stimulation of the respiratory immune system. In this paper the efficacy of two nanoparticle vaccines (poly-lactide-co-glycolide, PLGA; polymethylmethacrylate, PMMA), incorporating proteins of bovine parainfluenza type 3 virus (BPI-3) was investigated. As a preliminary to experiments in calves, it was considered essential to demonstrate immunogenicity of the experimental vaccine in mice. Mice immunised with PLGA nanoparticles, containing BPI-3 proteins, developed higher levels of virus-specific antibody than mice immunised with the PMMA vaccine or with soluble viral proteins alone. Immunoblotting using serum from the vaccinated mice, demonstrated strong reactions against the major BPI-3 proteins.     

The effects of GnRH and adrenergic agents on PRL and beta-endorphin secretion by porcine pituitary cells in vitro

The direct effects of alpha- and beta-adrenergic agents on PRL and beta-endorphin (beta-END) secretion in vitro by porcine pituitary cells have been investigated. Pituitary glands were obtained from mature gilts, which were ovariectomised (OVX) one month before slaughter. Ovariectomised gilts, assigned to four groups, were primed with: (1) vehicle (OVX); (2) and (3) oestradiol benzoate (EB; 2.5 mg/100 kg b.w.) at 30-36 h (OVX+EB I) and 60-66 h (OVX+EB II) before slaughter, respectively; and (4) progesterone (P4; 120 mg/100 kg b.w.) for 5 consecutive days before slaughter (OVX+P4). Isolated anterior pituitary cells were submitted to 3.5 h incubation in the presence of GnRH, alpha- and beta-adrenergic agonists [phenylephrine (PHEN) and isoproterenol (ISOP), respectively], or alpha- and beta-adrenergic blockers [phentolamine (PHENT) and propranolol (PROP), respectively]. The culture media were assayed for PRL (exp. I) and beta-endorphin-like immunoreactivity (beta-END-LI) (experiment II). In experiment I, GnRH did not influence PRL release by pituitary cells in all experimental groups. Some of tested doses of adrenergic agonists, PHEN and ISOP, increased PRL release from pituitary cells of OVX gilts, but not from those of OVX+EB I animals. In the OVX+EB II group, PHEN alone, but ISOP with PROP, potentiated PRL secretion by the cells. In OVX+P4 animals, PHEN alone or in combination with PHENT and also ISOP alone or with PROP enhanced PRL output from the cells. In experiment II, addition of GnRH increased beta-END-LI release from pituitary cells only in the OVX+EB II group. PHEN and PHENT potentiated beta-END-LI secretion by pituitary cells in OVX+EB II and OVX+P4 groups, while ISOP and PROP increased beta-END-LI secretion by the cells of OVX and OVX+EB II animals. In turn, in the OVX+EB I group, effect of PHENT and PROP on PRL secretion by pituitary cells was inhibitory. In conclusion, our results suggest that adrenergic agents can modulate PRL and beta-END secretion by porcine pituitary cells in a manner dependent on the hormonal status of gilts.     

The influence of restraint immobilization stress on the concentration qf bioamines and cortisol in plasma of Pietrain and Duroc pigs

Forty-five Duroc (recognized as not susceptible to stress) and 34 Pietrain (susceptible to stress) pigs were subjected to immobilization stress in a prone position for 5, 15, 30 and 60 min. Plasma concentrations of epinephrine (E), norepinephrine (NE), dopamine (DA) and cortisol (C) were determined in response to restraint stress. The concentrations of E, NE and DA were different between the two strains of pigs (some significant interactions); the highest response was seen after 5 min of stress. The concentration of plasma C increased with duration of stress and there was a significant interaction between strain of animals and the time of stress. Our data substantiate the use of E, NE, DA and C as indicators of stress in swine as early as 5 min after exposure to the stressor. It is also shown that stress-susceptible Pietrain pigs had higher plasma concentrations of E, NE and DA than Duroc pigs.     

Response of Sorghum to Abiotic Stresses: A Review

Sorghum [(Sorghum bicolor L.) Moench] is a highly productive crop plant, which can be used for alternative energy resource, human food, livestock feed or industrial purposes. The biomass of sorghum can be utilized as solid fuel via thermochemical routes or as a carbohydrate substrate via fermentation processes. The plant has a great adaptation potential to drought, high salinity and high temperature, which are important characteristics of genotypes growing in extreme environments. However, the climate change in the 21st century may bring about new challenges in the cultivated areas. In this review, we summarize the most recent literature about the responses of sorghum to the most important abiotic stresses: nutrient deficiency, aluminium stress, drought, high salinity, waterlogging or temperature stress the plants have to cope with during cultivation. The advanced molecular and system biological tools provide new opportunities for breeders to select stress‐tolerant and high‐yielding cultivars.     

杜仲种子发芽试验分析

杜仲为我国特有的经济树种和名贵中药，其皮为重要的中药材。采用传统的种子育苗法其发芽率一般不超过50％。将杜仲种子剥去果皮或剥去果皮并弃去胚根端部分胚乳，接种于Ms＋0．8％琼脂的培养基上进行发芽试验，种子发芽率分别为50％和94％。将种子分别置于10℃、15℃、20℃、25℃、30℃的温度下培养发现，杜仲的最适发芽温度为20℃。     

Localized graft incompatibility in pear/quince (Pyrus communis/Cydonia oblonga) combinations: multivariate analysis of histological data from 5-month-old grafts

To characterize the structural events associated with incompatibility of graft development, we conducted a histological study of compatible and incompatible pear/pear and pear/quince grafts that had been grown for five months in a greenhouse. Multivariate analysis of histological data describing the structure of the graft union allowed us to discriminate between compatible and incompatible combinations before either macroscopic examination or qualitative microscopic examination differences between graft combinations became evident. The histological variables responsible for the discrimination between incompatible and compatible unions were related to three typical symptoms of graft incompatibility: bark discontinuity, which was the main feature; cambial dysfunction; and accumulation of starch in the scion. Little cell necrosis was observed at the interface of incompatible grafts at the 5-month stage of graft development. Multivariate analysis of histological data provides a new tool for studying early structural events resulting from the graft incompatibility response and for diagnosing early graft incompatibility.     

Identification and quantification of seed carotenoids in selected wheat species

Selected primitive and modern wheat species were evaluated on the basis of their carotenoid composition and effects of the genotype and environment on lutein using spectrometry and liquid chromatography. Carotenoids in the wheat extracts were identified and confirmed on the basis of their UV/vis and mass spectra compared with those of authentic standards. The protonated molecule (M + 1)+ at m/z 569 was the predominant ion for zeaxanthin compared to the fragment ion at m/z 551 for lutein. A similar carotenoid profile was obtained for the wheat species investigated, but significant differences were observed in the concentration of carotenoids. Einkorn (Triticum monococcum) exhibited the highest level of all-trans-lutein, averaging 7.41 microg/g with small amounts of all-trans-zeaxanthin, cis-lutein isomers, and beta-carotene. Durum, Kamut, and Khorasan (Triticum turgidum) had intermediate levels of lutein (5.41-5.77 microg/g), while common bread or pastry wheat (Triticum aestivum) had the lowest content (2.01-2.11 microg/g). Lutein in einkorn appeared to be influenced significantly by environmental growing conditions.