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111.
M. A. Efimova A. V. Ivanov Kh. Z. Gaffarov A. I. Yarullin 《Russian Agricultural Sciences》2012,38(2):149-152
An ELISA test system was developed on the basis of using bovine parvovirus type I intended for revealing antibodies in bovine blood serum in sick, convalescent, and vaccinated animals. 相似文献
112.
Cryopreservation and In Vitro culture of Preimplantation Embryos in Djungarian Hamster (Phodopus sungorus)
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EYu Brusentsev TO Abramova IN Rozhkova TN Igonina VA Naprimerov NYu Feoktistova SYa Amstislavsky 《Reproduction in domestic animals》2015,50(4):677-683
Although embryo cryobanking was applied to Syrian golden and to Campbell's hamsters, no attempt has been made at freezing embryos in Djungarian hamsters. Four‐cell stage embryos were flushed from the reproductive ducts of pregnant females before noon of the third‐day post coitum and frozen in 0.25‐ml straws according to standard procedures of slow cooling. A mixture of permeating (ethylene glycol) and non‐permeating (sucrose) cryoprotectants was used. The thawing was performed by incubating at RT for 40 s followed by 40 s in a water bath at 30.0°C. Most (66.7%) of the non‐frozen four‐cell embryos developed up to the morula stage in rat one‐cell embryo culture medium (R1ECM). The use of hamster embryo culture medium (HECM) yielded fewer morulas (18.2%) during the same 24‐h period of culture. The rate of embryo's surviving the freezing–thawing procedures, as estimated by light microscopy, was 60.7–68.8%. After 24‐h culturing in R1ECM, 64.7% of frozen–thawed four‐cell embryos developed and all of them reached the morula stage. Supplementation of R1ECM with GM‐CSF (2 ng/ml) improved the rate of Djungarian hamster frozen–thawed embryo development: 100% of the four‐cell stage embryos developed, 50% of them achieved the morula stage, and 50% developed even further and reached the blastocyst stage within 24 h of culturing. This study reports the world's first successful transfer of frozen–thawed Djungarian hamster embryos yielding term pups. Taken together, the results of this study demonstrate the possibility of applying some key reproductive technologies, that is, embryo freezing/cryopreservation and in vitro culture, to Djungarian hamsters. 相似文献
113.
Huismans Y Rouzée A Gijsbertsen A Jungmann JH Smolkowska AS Logman PS Lépine F Cauchy C Zamith S Marchenko T Bakker JM Berden G Redlich B van der Meer AF Muller HG Vermin W Schafer KJ Spanner M Ivanov MY Smirnova O Bauer D Popruzhenko SV Vrakking MJ 《Science (New York, N.Y.)》2011,331(6013):61-64
Ionization is the dominant response of atoms and molecules to intense laser fields and is at the basis of several important techniques, such as the generation of attosecond pulses that allow the measurement of electron motion in real time. We present experiments in which metastable xenon atoms were ionized with intense 7-micrometer laser pulses from a free-electron laser. Holographic structures were observed that record underlying electron dynamics on a sublaser-cycle time scale, enabling photoelectron spectroscopy with a time resolution of almost two orders of magnitude higher than the duration of the ionizing pulse. 相似文献
114.
Renne PR Melosh HJ Farley KA Reimold WU Koeberl C Rampino MR Kelly SP Ivanov BA 《Science (New York, N.Y.)》2004,306(5696):610-2; author reply 610-2
115.
An inventory of published data on the natural evolution of forest-steppe, steppe, and semidesert soils in the southern part of the East European Plain and in the southern Trans-Ural region during the Subboreal period of the Holocene has been performed. Schematic maps of the thickness of soil humus profiles and the depth of soil carbonates have been developed for the chronosections of 5000–4200, 4100–3900, and 3800–3200 years ago. On this basis, the areas with specific patterns of natural evolution of soils in the Subboreal period are delineated. 相似文献
116.
117.
J. Encheva H. Köhler W. Friedt F. Tsvetkova P. Ivanov V. Encheva P. Shindrova 《Euphytica》2003,130(2):167-175
Immature zygotic embryos from the American fertility restorer line RHA-857 were used as donor material for induction of direct
organogenesis in sunflower (Helianthusannuus L.). The range of spontaneous somaclonal variation among the progenies of regenerants was studied. The genetic modifications
observed in regenerants included agronomic traits such as oil content in seed, 1000 seed weight, plant height, leaf width,
leaf length, petiole length, internode length, head diameter, number of branches, length of branches, number of ray florets,
seed width, seed length, and seed thickness. RAPD molecular analysis carried out on sunflower materials in the R-11 generation
showed the absence of a specific 358 bp band in somaclonal line 11/2/51 R. This line showed a modified architecture, full
resistance to Phomopsis helianthiand higher oil content in seed in comparison to the standard RHA-857. Line31/3/53 R was with modified architecture and higher
1000 seed weight. Hybrid No. 144 produced with the participation of somaclonal line 20/5/52 R demonstrated high production
capacity, shorter vegetation period and reduced height. The combination of these favourable changes is desirable in breeding
work on sunflower. Somaclonal variation through director ganogenesis has facilitated the creation of genetically heritable
variation in sunflower, which can be used with great success for hybrid seed production of highly productive hybrids.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
118.
G.?GeorgievEmail author V.?Sakalian K.?Ivanov P.?Boyadzhiev 《Journal of pest science》2004,77(3):151-153
Seven xylophagous insect species were established in stems and branches of Salix caprea L. during the period 1998–2003 in Bulgaria: Agrilus lineola lineola Redtenbacher (Coleoptera: Buprestidae), Aromia moschata (Linnaeus), Oberea oculata (Linnaeus), Saperda populnea (Linnaeus), Saperda similis Laicharting (Coleoptera: Cerambycidae), Synanthedon formicaeformis (Esper) (Lepidoptera: Sesiidae) and Rhabdophaga clavifex (Kieffer) (Diptera: Cecidomyiidae). From them, six parasitoid species were reared: Dolichomitus mesocentrus (Gravenhorst), Dolichomitus populneus (Ratzeburg) (Hymenoptera: Ichneumonidae), Stephanus serrator (Fabricius) (Hymenoptera: Stephanidae), Aprostocetus metra (Walker), Euderus caudatus (Thomson) (Hymenoptera: Eulophidae) and Billaea irrorata (Meigen) (Diptera: Tachinidae). D. populneus, E. caudatus and B. irrorata were connected with S. populnea; D. mesocentrus and S. serrator with S. similis; and A. metra with R. clavifex. Two parasitoids (A. metra and E. caudatus) had not previously been reported for the fauna of Bulgaria. The parasitoid–host relationships D. mesocentrus–S. similis and S. serrator –S. similis were established for the first time in this study. 相似文献
119.
D. A. Ivanov V. A. Tyulin O. V. Karaseva M. V. Rublyuk N. V. Grits 《Russian Agricultural Sciences》2013,39(3):264-267
Features of existential dynamics of a crop of spring wheat of grade Lada within the limits of agro-landscape moraine hills are investigated. Laws of influence of agroclimatic and landscape conditions on efficiency of culture are revealed. Recommendations on adaptation of manufacture of grain to landscape conditions are given. 相似文献
120.