首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   848篇
  免费   48篇
林业   34篇
农学   18篇
基础科学   5篇
  118篇
综合类   170篇
农作物   37篇
水产渔业   59篇
畜牧兽医   373篇
园艺   17篇
植物保护   65篇
  2023年   6篇
  2022年   8篇
  2021年   21篇
  2020年   23篇
  2019年   26篇
  2018年   22篇
  2017年   19篇
  2016年   20篇
  2015年   16篇
  2014年   12篇
  2013年   56篇
  2012年   31篇
  2011年   53篇
  2010年   33篇
  2009年   17篇
  2008年   36篇
  2007年   39篇
  2006年   29篇
  2005年   23篇
  2004年   37篇
  2003年   26篇
  2002年   13篇
  2001年   10篇
  2000年   15篇
  1999年   9篇
  1998年   6篇
  1995年   5篇
  1992年   11篇
  1991年   14篇
  1990年   9篇
  1989年   18篇
  1988年   26篇
  1987年   27篇
  1986年   25篇
  1983年   7篇
  1982年   8篇
  1979年   4篇
  1976年   6篇
  1975年   4篇
  1973年   5篇
  1972年   6篇
  1971年   5篇
  1970年   5篇
  1968年   4篇
  1967年   5篇
  1959年   5篇
  1949年   5篇
  1948年   6篇
  1946年   5篇
  1896年   3篇
排序方式: 共有896条查询结果,搜索用时 15 毫秒
81.
The levels of hydrophilic, lipophilic, and enzymatic antioxidants, the oxidative damage to lipids and proteins, and the fatty acid patterns of triglyceride and phospholipid fractions were assayed in fresh muscle tissue of rainbow trouts (Oncorhynchus mykiss) and sea basses (Dicentrarchus labrax) during aging, to investigate the correlation between oxidative stress and aging processes in fish. The present studies suggests that lipid peroxidation and accumulation of oxidized proteins during in vivo aging are most likely to be linked with an age-dependent decline of lipophilic antioxidants (CoQH(2), CoQ, and vitamin E) and vitamin C contents in muscle tissue, whereas fish aging is not linked to a decline in antioxidant enzymes and reduced glutathione levels. Lipophilic antioxidant and vitamin C levels represent a reliable marker of oxidative stress during aging, and their determination might be useful for the assessment of fish age.  相似文献   
82.
Hexane extracts of epicuticular wax from cv. Gala apples were noted to have an unusual, broad absorbance maximum at approximately 258 nm, which led us to isolate and identify the primary UV-absorbing compounds. Column and thin-layer chromatography yielded a fraction that gave a series of paired, 260-nm-absorbing peaks on C(18) HPLC. These were shown to be a family of phenolic fatty acid esters, for which retention times increased with increasing fatty acid chain length, and paired peaks were esters of two related phenolics with the same fatty acid moiety. Alkaline hydrolysis of the esters released two water-soluble phenolics separable by C(18) HPLC. Electrospray ionization mass spectrometry gave a molecular mass of 150 for both, and (1)H NMR plus UV absorbance spectra identified them as E and Z isomers of p-coumaryl alcohol. Alkaline cleavage of the fatty acid esters in the presence of methanol or ethanol resulted in partial derivatization of E-p-coumaryl alcohol to the corresponding gamma-O-methyl or O-ethyl ether. Gradient HMQC NMR of the HPLC-purified stearate ester of E-p-coumaryl alcohol indicated that fatty acid esterification occurs at the gamma-OH rather than at the 4-OH on the phenyl ring. This is the first report of fatty acid esters of monolignols as a natural plant product.  相似文献   
83.
The effect of red and white wine total extracts and phenolic fractions on heat shock protein (Hsp) levels in tumor cells and on tumor and endothelial cell populations in vitro has been investigated. Total extracts of red wines decreased Hsp70 and Hsp27 levels and the numbers of tumor and endothelial cells. Several red and white wine fractions significantly decreased Hsp27 levels, and some of them had also an effect on Hsp70 levels. A red wine fraction rich in polymeric flavanols and a white wine one rich in phenolic acids, flavonols, and tyrosol strongly lowered Hsp27 levels. Some red and white wine fractions strongly reduced tumor cell numbers, whereas most of them decreased endothelial cell numbers to variable extents. The present results indicate that wine phenolics decrease Hsp levels in tumor cells and tumor and endothelial cell populations. These properties may be important in the potent anticarcinogenic action of wine phenolics.  相似文献   
84.
The deduced amino acid variability for the 10 kDa prolamin was determined for 16 Oryza species, both cultivated (rice) and wild. Prolamin, a seed storage protein and site of nitrogen and sulfur accumulation, is sequestered in the subaleurone layer of the starchy endosperm for use during seedling germination. The 10 kDa prolamin amino acid distribution for the cultivated species (O. sativa and O. glaberrima) was determined and compared to those of wild and, hitherto unknown, noncultivated Oryza species. Four wild species (O. granulata, O. australiensis, O. brachyantha, and O. meyeriana) exhibited the greatest residue heterogeneity in both the signal and mature peptide regions. A breakdown of the essential amino acid variance among three Central/South American and one African endemic wild species is also presented and compared with those of rice.  相似文献   
85.
86.
Borrelia anserina (Sakharoff) was successfully grown in a liquid medium (Barbour-Stoenner-Kelly) for 39 passages. By the 12th serial passage in medium, infectivity of B anserina for chicks was lost. Electron microscopy did not reveal structural differences between non-infective and infective cultured organisms. Changes in the protein profiles were found by electrophoresis as the organisms were passed in culture.  相似文献   
87.
Control ofSclerotinia sclerotiorum in muskmelons was obtained by a single soil drench with benomyl. The material was found in the apical parts of the plant and in the soil until the end of the growing season. Benomyl [methyl l-(butylcarbamoyl)-2-benzi-midazolecarbamate] and thiophanate methyl-NF 44 [1.2- bis (3 methoxycarbonyl-2-thioureido) benzene], applied to seedbeds, were taken up by and then persisted in tomato, pepper and eggplant for approximately 8 weeks after application. Thiophanate-NF 35 [1.2-bis (ethoxycarbonyl-2-thioureido) benzene] could be detected only in the bottom leaves of pepper and eggplant, for up to 4 weeks after application.  相似文献   
88.
A study has been made of the intestinal flora of chickens aged between 2 and 6½ weeks. No major differences were found when rations containing 9 or 25% fish meal were compared or when two different sources of fish meal were used. Throughout the period investigated, it was confirmed that the lactobacilli are the only group of organisms generally present in the small intestine in numbers exceeding 104/g. Clostridia were regularly found at 102 to 104/g, but Clostridium welchii was isolated from only occasional samples.

In the caeca, the total number of bacteria present at 2, 3, 4 and 6½ weeks was about 1011/g. Of these it was generally possible to isolate more than 20% using an anaerobic roll‐tube technique and oxygen‐free CO2. Many different types of anaerobes were isolated including strictly anaerobic budding bacteria which had not previously been studied. During the growth of the bird the flora changed. Anaerobic streptococci (peptostreptococci) predominated at 2 weeks but gradually decreased so that by 6½ weeks these organisms formed only a small part of the flora. Many of the Gram‐negative non‐sporing anaerobes only appeared between the 4th and 6th weeks.

Throughout the 2 to 6½‐week period all the caecal samples examined contained 108 to 109 anaerobes/g which were able to attack uric acid and sometimes 1010/g. This property was shared by at least one group of peptostreptococci, four groups of Gram‐negative non‐sporing anaerobes, three groups of Gram‐variable or Gram‐positive anaerobic non‐sporing rods and several species of clostridia.  相似文献   

89.
No clinical signs, gross lesions, or increased mortality were observed in specific-pathogen-free chickens orally inoculated at 5 days of age with Cryptosporidium baileyi, reovirus 2035, reovirus 2408, or combinations of these agents. Weight gain of chickens inoculated with only reovirus 2408 was depressed 0-8 days postinoculation (PI) (P less than 0.01) but not for the 21-day period PI. Weight gain of chickens inoculated with only reovirus 2035 was not affected. Cryptosporidium baileyi infection significantly depressed weight gain 8-14 days PI but not for the entire 21-day period PI. Weight gain of chickens infected with both C. baileyi and reovirus 2035 was significantly depressed 0-14 days PI and for the entire 21-day period PI. Dual infection with C. baileyi and either reovirus appeared to promote shedding of both agents. Cryptosporidia were found principally in the rectum 2-10 days PI and in the bursa of Fabricius 6-10 days PI. Reovirus infection did not cause any microscopic lesions and did not modify lesions caused by C. baileyi infection.  相似文献   
90.
The effect of selection for milk yield on lactation yield, net energy balance, and on plasma growth hormone, insulin, prolactin, nonesterified fatty acids and glucose was studied in two groups of primiparous Holstein cows of differing genetic merit. Net energy balance was calculated and serial blood samples were collected for a 7 hr period at 0, 45, 90 and 180 days postpartum. Growth hormone releasing factor (.2 μg/kg BW) was administered after 2.5 hr at 0, 45 and 180 days postpartum, while epinephrine (.7 μg/kg BW) was administered at 90 days postpartum, Milk yield was greater, net energy balance was decreased and plasma growth hormone was greater in genetically superior selection group cows compared to control cows.

Growth hormone showed similar increases in both genetic groups in response to growth hormone releasing factor, while prolactin, insulin and glucose were not altered. Epinephrine stimulated an increase in plasma nonesterified fatty acid, glucose and insulin, but responses did not differ between genetic groups. Results indicate differences exist in production efficiency, net energy balance and plasma growth hormone concentration among dairy cattle as a result of selection for milk yield and suggest that selection pressure may act to alter homeorhetic control of nutrient metabolism.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号