全文获取类型
收费全文 | 1356篇 |
免费 | 70篇 |
国内免费 | 2篇 |
专业分类
林业 | 96篇 |
农学 | 35篇 |
基础科学 | 16篇 |
236篇 | |
综合类 | 185篇 |
农作物 | 34篇 |
水产渔业 | 147篇 |
畜牧兽医 | 479篇 |
园艺 | 32篇 |
植物保护 | 168篇 |
出版年
2023年 | 6篇 |
2022年 | 10篇 |
2021年 | 25篇 |
2020年 | 22篇 |
2019年 | 33篇 |
2018年 | 30篇 |
2017年 | 30篇 |
2016年 | 40篇 |
2015年 | 27篇 |
2014年 | 38篇 |
2013年 | 45篇 |
2012年 | 91篇 |
2011年 | 82篇 |
2010年 | 56篇 |
2009年 | 45篇 |
2008年 | 100篇 |
2007年 | 97篇 |
2006年 | 90篇 |
2005年 | 72篇 |
2004年 | 80篇 |
2003年 | 62篇 |
2002年 | 64篇 |
2001年 | 30篇 |
2000年 | 15篇 |
1999年 | 17篇 |
1998年 | 18篇 |
1997年 | 16篇 |
1996年 | 14篇 |
1995年 | 12篇 |
1994年 | 10篇 |
1993年 | 8篇 |
1992年 | 10篇 |
1991年 | 5篇 |
1990年 | 5篇 |
1989年 | 12篇 |
1988年 | 3篇 |
1987年 | 3篇 |
1986年 | 3篇 |
1985年 | 13篇 |
1984年 | 15篇 |
1983年 | 13篇 |
1982年 | 7篇 |
1981年 | 4篇 |
1980年 | 8篇 |
1978年 | 4篇 |
1976年 | 5篇 |
1973年 | 5篇 |
1972年 | 3篇 |
1961年 | 2篇 |
1942年 | 3篇 |
排序方式: 共有1428条查询结果,搜索用时 93 毫秒
111.
Donnelly SE Birtcher RC Allen CW Morrison I Furuya K Song M Mitsuishi K Dahmen U 《Science (New York, N.Y.)》2002,296(5567):507-510
High-resolution transmission electron microscopy images of room-temperature fluid xenon in small faceted cavities in aluminum reveal the presence of three well-defined layers within the fluid at each facet. Such interfacial layering of simple liquids has been theoretically predicted, but observational evidence has been ambiguous. Molecular dynamics simulations indicate that the density variation induced by the layering will cause xenon, confined to an approximately cubic cavity of volume approximately 8 cubic nanometers, to condense into the body-centered cubic phase, differing from the face-centered cubic phase of both bulk solid xenon and solid xenon confined in somewhat larger (>/=20 cubic nanometer) tetradecahedral cavities in face-centered cubic metals. Layering at the liquid-solid interface plays an important role in determining physical properties as diverse as the rheological behavior of two-dimensionally confined liquids and the dynamics of crystal growth. 相似文献
112.
Stewart I 《Science (New York, N.Y.)》2002,296(5574):1802-3; author reply 1802-3
113.
van Schaik CP Ancrenaz M Borgen G Galdikas B Knott CD Singleton I Suzuki A Utami SS Merrill M 《Science (New York, N.Y.)》2003,299(5603):102-105
Geographic variation in some aspects of chimpanzee behavior has been interpreted as evidence for culture. Here we document similar geographic variation in orangutan behaviors. Moreover, as expected under a cultural interpretation, we find a correlation between geographic distance and cultural difference, a correlation between the abundance of opportunities for social learning and the size of the local cultural repertoire, and no effect of habitat on the content of culture. Hence, great-ape cultures exist, and may have done so for at least 14 million years. 相似文献
114.
Siegert MJ Welch B Morse D Vieli A Blankenship DD Joughin I King EC Vieli GJ Payne AJ Jacobel R 《Science (New York, N.Y.)》2004,305(5692):1948-1951
Upstream of Byrd Station (West Antarctica), ice-penetrating radar data reveal a distinctive fold structure within the ice, in which isochronous layers are unusually deep. The fold has an axis more than 50 kilometers long, which is aligned up to 45 degrees to the ice flow direction. Although explanations for the fold's formation under the present flow are problematic, it can be explained if flow was parallel to the fold axis approximately 1500 years ago. This flow change may be associated with ice stream alterations nearer the margin. If this is true, central West Antarctica may respond to future alterations more than previously thought. 相似文献
115.
Stevens J Corper AL Basler CF Taubenberger JK Palese P Wilson IA 《Science (New York, N.Y.)》2004,303(5665):1866-1870
The 1918 "Spanish" influenza pandemic represents the largest recorded outbreak of any infectious disease. The crystal structure of the uncleaved precursor of the major surface antigen of the extinct 1918 virus was determined at 3.0 angstrom resolution after reassembly of the hemagglutinin gene from viral RNA fragments preserved in 1918 formalin-fixed lung tissues. A narrow avian-like receptor-binding site, two previously unobserved histidine patches, and a less exposed surface loop at the cleavage site that activates viral membrane fusion reveal structural features primarily found in avian viruses, which may have contributed to the extraordinarily high infectivity and mortality rates observed during 1918. 相似文献
116.
Barrios-Rodiles M Brown KR Ozdamar B Bose R Liu Z Donovan RS Shinjo F Liu Y Dembowy J Taylor IW Luga V Przulj N Robinson M Suzuki H Hayashizaki Y Jurisica I Wrana JL 《Science (New York, N.Y.)》2005,307(5715):1621-1625
Signaling pathways transmit information through protein interaction networks that are dynamically regulated by complex extracellular cues. We developed LUMIER (for luminescence-based mammalian interactome mapping), an automated high-throughput technology, to map protein-protein interaction networks systematically in mammalian cells and applied it to the transforming growth factor-beta (TGFbeta) pathway. Analysis using self-organizing maps and k-means clustering identified links of the TGFbeta pathway to the p21-activated kinase (PAK) network, to the polarity complex, and to Occludin, a structural component of tight junctions. We show that Occludin regulates TGFbeta type I receptor localization for efficient TGFbeta-dependent dissolution of tight junctions during epithelial-to-mesenchymal transitions. 相似文献
117.
Perry SJ Baillie GS Kohout TA McPhee I Magiera MM Ang KL Miller WE McLean AJ Conti M Houslay MD Lefkowitz RJ 《Science (New York, N.Y.)》2002,298(5594):834-836
Catecholamines signal through the beta2-adrenergic receptor by promoting production of the second messenger adenosine 3',5'-monophosphate (cAMP). The magnitude of this signal is restricted by desensitization of the receptors through their binding to beta-arrestins and by cAMP degradation by phosphodiesterase (PDE) enzymes. We show that beta-arrestins coordinate both processes by recruiting PDEs to activated beta2-adrenergic receptors in the plasma membrane of mammalian cells. In doing so, the beta-arrestins limit activation of membrane-associated cAMP-activated protein kinase by simultaneously slowing the rate of cAMP production through receptor desensitization and increasing the rate of its degradation at the membrane. 相似文献
118.
Sreedharan J Blair IP Tripathi VB Hu X Vance C Rogelj B Ackerley S Durnall JC Williams KL Buratti E Baralle F de Belleroche J Mitchell JD Leigh PN Al-Chalabi A Miller CC Nicholson G Shaw CE 《Science (New York, N.Y.)》2008,319(5870):1668-1672
Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disorder characterized pathologically by ubiquitinated TAR DNA binding protein (TDP-43) inclusions. The function of TDP-43 in the nervous system is uncertain, and a mechanistic role in neurodegeneration remains speculative. We identified neighboring mutations in a highly conserved region of TARDBP in sporadic and familial ALS cases. TARDBPM337V segregated with disease within one kindred and a genome-wide scan confirmed that linkage was restricted to chromosome 1p36, which contains the TARDBP locus. Mutant forms of TDP-43 fragmented in vitro more readily than wild type and, in vivo, caused neural apoptosis and developmental delay in the chick embryo. Our evidence suggests a pathophysiological link between TDP-43 and ALS. 相似文献
119.
Armstead I Donnison I Aubry S Harper J Hörtensteiner S James C Mani J Moffet M Ougham H Roberts L Thomas A Weeden N Thomas H King I 《Science (New York, N.Y.)》2007,315(5808):73
A key gene involved in plant senescence, mutations of which partially disable chlorophyll catabolism and confer stay-green leaf and cotyledon phenotypes, has been identified in Pisum sativum, Arabidopsis thaliana, and Festuca pratensis by using classical and molecular genetics and comparative genomics. A stay-green locus in F. pratensis is syntenically equivalent to a similar stay-green locus on rice chromosome 9. Functional testing in Arabidopsis of a homolog of the rice candidate gene revealed (i) senescence-associated gene expression and (ii) a stay-green phenotype after RNA interference silencing. Genetic mapping in pea demonstrated cosegregation with the yellow/green cotyledon polymorphism (I/i) first reported by Gregor Mendel in 1866. 相似文献
120.
Zelaya IA Anderson JA Owen MD Landes RD 《Journal of agricultural and food chemistry》2011,59(6):2202-2212
Endogenous shikimic acid determinations are routinely used to assess the efficacy of glyphosate in plants. Numerous analytical methods exist in the public domain for the detection of shikimic acid, yet the most commonly cited comprise spectrophotometric and high-pressure liquid chromatography (HPLC) methods. This paper compares an HPLC and two spectrophotometric methods (Spec 1 and Spec 2) and assesses the effectiveness in the detection of shikimic acid in the tissues of glyphosate-treated plants. Furthermore, the study evaluates the versatility of two acid-based shikimic acid extraction methods and assesses the longevity of plant extract samples under different storage conditions. Finally, Spec 1 and Spec 2 are further characterized with respect to (1) the capacity to discern between shikimic acid and chemically related alicyclic hydroxy acids, (2) the stability of the chromophore (t1/2), (3) the detection limits, and (4) the cost and simplicity of undertaking the analytical procedure. Overall, spectrophotometric methods were more cost-effective and simpler to execute yet provided a narrower detection limit compared to HPLC. All three methods were specific to shikimic acid and detected the compound in the tissues of glyphosate-susceptible crops, increasing exponentially in concentration within 24 h of glyphosate application and plateauing at approximately 72 h. Spec 1 estimated more shikimic acid in identical plant extract samples compared to Spec 2 and, likewise, HPLC detection was more effective than spectrophotometric determinations. Given the unprecedented global adoption of glyphosate-resistant crops and concomitant use of glyphosate, an effective and accurate assessment of glyphosate efficacy is important. Endogenous shikimic acid determinations are instrumental in corroborating the efficacy of glyphosate and therefore have numerous applications in herbicide research and related areas of science as well as resolving many commercial issues as a consequence of glyphosate utilization. 相似文献