Surgical outcome and ocular complications of evisceration and intraocular prosthesis implantation in dogs with end stage glaucoma: a review of 20 cases

Surgical outcome and complications following evisceration and implantation of intraocular silicone prosthesis (ISP) in 20 canine eyes with end-stage glaucoma were analyzed, including clinical signs, complications, cosmetic appearance, and owners' responses. The mean postoperative follow-up time was 312 days. Major short-term surgical complications were central corneal ulceration (6/20) and infections (3/20), while long-term complication was keratoconjunctivitis sicca (KCS) (2/20). All patient owners (100%) showed satisfied with the surgical outcome and postoperative cosmetic effect including 85% of the owners gave an excellent or good rating of satisfaction. No more medication needed for long term control except the KCS cases. With careful case selection and post-operative care, evisceration with ISP implantation proves a good and safe surgical procedure with minimal complications for end stage glaucoma.     

黄山市对做好旅游城市动物卫生监督工作的探索

黄山市位于安徽省南部,境内旅游资源丰富,景观独特,以奇松、怪石、云海、温泉"四绝"著称于世,1985年,黄山作为唯一的山岳风光,被评选为全国十大风景名胜之一。2010年旅游接待量达到1001.3万     

蛋白质营养水平对工蜂初生重和咽下腺发育的影响

利用不同配比的茶花粉和黄豆粉饲料饲喂蜂群,测定后代工蜂的初生重和咽下腺宽度。结果发现,饲喂100％茶花粉与饲喂“75％茶花粉＋25％黄豆粉”的蜂群,所育工蜂的初生重差异不显著,与饲喂100％黄豆粉的蜂群相比,差异极显著;饲喂100％茶花粉与饲喂“75％茶花粉＋25％黄豆粉”的蜂群相比,6日龄、9日龄、12日龄工蜂咽下腺宽度差异均不显著,与饲喂100％黄豆粉的蜂群相比,6日龄、9日龄差异不显著,而12日龄差异显著。本试验结果表明,蛋白质营养水平对工蜂初生重和咽下腺发育均具有显著的影响。     

γ-氨基丁酸对夏季生长肥育猪血清生化指标的影响

将48头体重43kg左右的"杜×长×大"三元杂交猪随机分成两组,饲喂在基础日粮中添加0、10mg/kgGABA的日粮。结果表明:添加GABA使血清中钙、磷、钾、氯4种无机离子浓度提高,差异不显著(P>0.05);总蛋白、白蛋白、尿素氮水平有提高趋势,差异不显著(P>0.05),葡萄糖水平提高13.29%,差异显著(P<0.05);丙氨酸转氨酶、天门冬氨酸转氨酶活性上升,差异不显著(P>0.05),乳酸脱氢酶、肌酸磷酸激酶活性分别下降16.93%(P<0.05)、38.22%(P<0.01);谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性均有上升,其中GSH-Px差异极显著(P<0.01),丙二醛含量下降(P>0.05)。     

雄蜂蛹中硝基呋喃类代谢物含量的测定及超标原因分析

雄蜂蛹中硝基呋喃类抗生素尤其是呋喃西林代谢物-氨基脲(SEM)残留超标是困扰我国雄蜂蛹出口的一个难题。本文对不同日龄雄蜂蛹中硝基呋喃类代谢物含量进行了测定,并对超标原因进行了分析。测定结果表明,在蜂群没有使用任何硝基呋喃类药物情况下,各日龄雄蜂蛹中的SEM含量依然严重超标,随着日龄的增长,雄蜂蛹中的SEM含量大幅增长,特别是16d以后,每过2d,含量增加2μg/kg左右。推测雄蜂蛹中SEM含量超标是非人为因素造成的,可能与雄蜂蛹生长后期体内甲壳素含量的增加相关。     

江都市生猪定点屠宰场动物防疫规范

畜牧兽医站与屠宰场是管理和被管理的关系。畜牧兽医站依据《动物防疫法》、《江苏省动物防疫条例》等法律、法规履行对动物防疫条件审核，对防疫、检疫法规执行情况检查，对屠宰场防疫工作进行督查、督办，并派出专职检疫员实施待宰动物入场检疫、宰前检疫、宰后检疫和检疫记录、有关防疫资料的核查和监督病害畜禽及其产品的处理等     

富含乳脂肪球膜蛋白乳清粉中总磷脂的含量测定方法研究

建立富含乳脂肪球膜蛋白乳清粉中测定总磷脂的方法,采用甲醇-三氯甲烷提取,经过高温灼烧,用钼蓝比色法测定无机磷的含量,再乘以系数26.31折算成试样中总磷脂的含量,该法标曲线性关系良好(R2=0.9990),回收率＞90%,RSD<3%。该法简单,准确率高,可用于富含乳脂肪球膜蛋白中总磷脂的测定。     

Expression kinetics of chicken β2-microglobulin and Class I MHC in vitro and in vivo during Marek’s disease viral infections

Marek’s disease virus (MDV) is a highly cell-associated herpesvirus that causes a disease in chickens characterized by tumor formation and immunosuppression. The changes of major histocompatibility complex (MHC) expression in different MDV-infected cells are not completely understood. In this study, we investigated the expression of the Class I MHC and β2-microglobulin (β2m) genes in response to MDV infection at different time points by real-time PCR. In both in vitro and in vivo, the expression levels of Class I MHC and β2m genes were upregulated during early MDV infections in comparison to control cells; We also found that the expression of Class I MHC gene was downregulated in BudR (5-bromo-2′-deoxyuridine)-treated MSB1 cells at 48 h and MDV-infected chicken embryo fibroblast cells (CEF) at 120 and 168 h post infection (hpi); Furthermore, compared to control groups, Class I MHC and β2m expression levels were downregulated in peripheral blood lymphocytes (PBLC) from MDV-infected chickens at 14 and 28 days post infection (dpi); Interestingly, both Class I MHC and β2m gene expression levels increased again in PBLC from MDV RB1B-infected chickens at 35 dpi, in which MDV was in the latent or transformed infection stages. In addition, Class I MHC expression was clearly decreased in MDV-infected CEF at 120 hpi although β2m expression was significantly increased. These changes in Class I MHC and β2m gene expression might provide more insights into host-virus interaction.     

区域性饲料企业:生存,必须勤练内功——原料篇

<正>目前,中国饲料业虽然呈激烈竞争态势,但人均饲料占有量远远达不到欧美国家的水平,饲料入户率也比较低。同时,虽然一些一线品牌企业在业内声名赫赫,但却还没有一家企业     

抗猪附红细胞体单克隆抗体的制备

以纯化的猪附红细胞体免疫BALB/c小鼠,运用淋巴细胞杂交瘤技术进行细胞融合,并用间接ELISA方法进行筛选,经过间接ELISA方法、免疫印迹和间接免疫荧光试验进行鉴定,共获得5株分泌抗猪附红细胞体单克隆抗体的杂交瘤细胞,分别命名为1H1、1H2、3A5、5B1和7E11,其单抗亚类鉴定分别属于IgG2b、IgG1、IgG2b、IgG2b和IgG2b。这5株McAb均能与猪附红细胞体全菌蛋白发生特异性反应,而不与猪肺炎支原体、猪链球菌、大肠杆菌和猪繁殖与呼吸综合征病毒发生反应,并且1H1、3A5和5B1能识别同一抗原位点,1H2和7E11识别另一抗原位点。