Physiological response,blood chemistry profile and mucus secretion of red sea bream (<Emphasis Type="Italic">Pagrus major</Emphasis>) fed diets supplemented with <Emphasis Type="Italic">Lactobacillus rhamnosus</Emphasis> under low salinity stress

Environmental stressors caused by inadequate aquaculture management strategies suppress the immune response of fish and make them more susceptible to diseases. Therefore, efforts have been made to relieve stress in fish by using various functional feed additives in the diet, including probiotics. The present work evaluates the effects of Lactobacillus rhamnosus (LR) on physiological stress response, blood chemistry and mucus secretion of red sea bream (Pagrus major) under low salinity stress. Fish were fed four diets supplemented with LR at [0 (LR0), 1 × 10² (LR1), 1 × 10⁴ (LR2) and 1 × 10⁶ (LR3) cells g^?1] for 56 days. Before stress, blood cortisol, urea nitrogen (BUN) and total bilirubin (T-BIL) showed no significant difference (P > 0.05), whereas plasma glucose and triglyceride (TG) of fish-fed LR2 and LR3 diets were significantly lower (P < 0.05) than those of the other groups. Plasma total cholesterol (T-CHO) of fish-fed LR3 diet was significantly (P < 0.05) lower than that of the other groups. Furthermore, total plasma protein, mucus myeloperoxidase activity and the amount of mucus secretion were significantly enhanced in LR-supplemented groups when compared with the control group (P < 0.05). After the application of the low salinity stress test, plasma cortisol, glucose, T-CHO and TG contents in all groups showed an increased trend significantly (P < 0.01) compared to the fish before the stress challenge. However, plasma total protein and the amount of secreted mucus showed a decreased trend in all groups. On the other hand, BUN, T-BIL and mucus myeloperoxidase activity showed no significant difference after exposure to the low salinity stress (P > 0.05). In addition, the fish that received LR-supplemented diets showed significantly higher tolerance against low salinity stress than the fish-fed LR-free diet (P < 0.05). The physiological status and the detected immune responses, including total plasma protein and mucus myeloperoxidase activity in red sea bream, will provide a more comprehensive outlook of the effects of probiotics to relieve stress in fish.     

Characterization of <Emphasis Type="Italic">kiss2</Emphasis> and <Emphasis Type="Italic">kissr2</Emphasis> genes and the regulation of kisspeptin on the HPG axis in <Emphasis Type="Italic">Cynoglossus semilaevis</Emphasis>

Reproduction allows organisms to produce offspring. Animals shift from immature juveniles into mature adults and become capable of sexual reproduction during puberty, which culminates in the first spermiation and sperm hydration or ovulation. Reproduction is closely related to the precise control of the hypothalamic–pituitary–gonadal (HPG) axis. Kisspeptin peptides are considered as the important regulator of HPG axis in mammalian. However, the current understanding of kisspeptin in flatfish is not comprehensive. In this study, we cloned and analyzed the kiss2 and kissr2 genes in Cynoglossus semilaevis. Interesting alternative splicing in the 5′-untranslated regions (UTR) of the Cskissr2 gene was found. The expression profiles of Cskiss2 and Cskissr2 showed relative high messenger RNA (mRNA) levels at the late gastrula stage during embryonic development, at total length = 40 mm during early gonadal differentiation, and in the brains and gonads of all investigated tissues. These results suggested that the kisspeptin system participated in embryogenesis and in the regulation of gonadal differentiation and development. Considering that the control and regulatory mechanisms of kisspeptin in the central reproductive axis are still unclear, we documented that the intramuscular injection of kisspeptin caused different sGnRH and cGnRH mRNA levels in a dose- and tissue-dependent manner. The mRNA expressions of FSH and LH were stimulated in the ovary and were inhibited in the testis under the kisspeptin treatments. These results provided foundations for understanding the roles of kisspeptin in the neuroendocrine system in fish. The manipulation of the kisspeptin system may provide new opportunities to control the gonadal development and even reproduction in fish.     

Plasma levels of immune factors and sex steroids in the male seahorse <Emphasis Type="Italic">Hippocampus erectus</Emphasis> during a breeding cycle

To better understand the endocrine- and immune-response pattern during reproduction in a fish species having parental care behaviors and also to accumulate the endocrine- and immune-related data for future explanations of the low reproductive efficiency in seahorse species, the variations of immune factors and sex steroids in the plasma of the male lined seahorse Hippocampus erectus at different breeding stages, i.e., pre-pregnancy, pregnancy (early, middle, and late periods), and post-pregnancy, were investigated in the present study. The immune factors included monocytes/leucocytes (M/L), leucocyte phagocytic rate (LPR), immunoglobulin M (Ig M), interleukin-2 (IL-2), interferon-α (IFN-α), and lysozyme (LZM). The sex steroids included testosterone (T), 11-ketotestosterone (11-KT), 11β-hydroxytestosterone (11β-OHT), 17α-methyltestosterone (17α-MT), 17β-estradiol (E2), and 17α-hydroxy-20β-dihydroprogesterone (17α-20β-P). Moreover, the immune metabolic activity of epithelium cells in the brood pouch at different breeding stages was also analyzed through ultrastructural observations of the abundance of cytoplasmic granules, mitochondria, endoplasmic reticulum, lysosomes, and exocytosis. The results show that a higher immune level was observed during pregnancy, particularly in the early and middle periods, and a lower immune level was noted during pre-pregnancy. Correspondingly, the epithelium cells in the brood pouch also showed a stronger immune metabolic activity during pregnancy and weaker activity during pre-pregnancy. Four sex steroids of T, 11β-OHT, 17α-MT, and E2 were higher during pre-pregnancy and lower during post-pregnancy, whereas 11-KT and 17α-20β-P, which were positively correlated with part immune factors, were higher during pregnancy. No negative correlations between sex steroids and immune factors were observed. In conclusion, the higher immune competence during pregnancy may indicate that parental care could improve immunity, which may be the major factor for no immunosuppressive effect of sex steroids during reproduction in the seahorse H. erectus, unlike noncaregiving fishes in which inhibitions of sex steroids on immunity are frequently observed. Moreover, higher 11-KT and 17α-20β-P during pregnancy than during pre-pregnancy and post-pregnancy may suggest that these two steroids are also involved in parental care regulation.     

军曹鱼幼鱼耗氧率与窒息点的研究

对平均体质量为16.0g～18.4g的军曹鱼在不同水温和盐度条件下的耗氧率进行了测定。结果表明,水温对军曹鱼的耗氧率有显著影响。盐度29‰时,水温从17℃上升到32℃,军曹鱼的耗氧率从0.357mg/g·h增加到0.880mg/g·h,水温(X)与耗氧率(Y)的关系可用幂函数表示:Y=0.0045X1.5197。在水温29℃时,军曹鱼的耗氧率随着盐度的变化而变化。此外,军曹鱼的耗氧率表现出较明显的昼夜变化规律,在中午前和傍晚后较高。水温对军曹鱼幼鱼的窒息点有显著影响。水温从21℃升高到33℃,窒息点从0.74mg/L增加到1.44mg/L。盐度对窒息点的影响不显著。     

影响配合饲料混合效果的因素及其控制

通过对影响配合饲料混合效果因素的分析，提出相应的控制措施，以提高混合机的生产率和饲料质量的稳定性。     

新时代湖泊渔业可持续发展的实践与思考(下)

五、突出湖泊渔业的产业属性,不断提升一二三产融合发展质量水平新时代湖泊渔业发展必然以最小的一产规模,通过与二三产业的充分融合,实现最大的经济效益。长期以来,依湖而居、以渔为生的传统渔民,大多采取守株待兔的销售方式,既无力进行产业链延伸,也不会创建自主品牌。湖泊渔业产业素质较低,体量将会因一产大规模压缩而迅速萎缩,必须采取措施,促进湖泊渔业转型升级。     

膜下滴灌结合暗管排水技术改良新疆盐碱地效果综述

膜下滴灌技术的大面积推广和应用,为新疆乃至西北干旱地区作物高产提供了强大的技术支撑。应用在盐碱地改良中,膜下滴灌“有灌无排”模式仅调节耕层盐分分布,并不能将盐分排出土体,长年灌溉容易引起爆发式积盐的风险,而暗管排水是目前公认的改良盐碱地最直接、最有效的方法。目前,将膜下滴灌与暗管排水有机结合形成的新型灌溉技术,充分利用膜下滴灌良好的抑蒸增温、节水抑盐等优势,同时利用暗管将淋洗盐水及时排出土体,使盐碱化土层产生新的水盐平衡,已在新疆盐碱地区开发利用中得到长足发展。本文重点分析并总结了新疆灌区膜下滴灌结合暗管排水系统的设计参数及土壤水盐运移规律,并综述了土壤理化性质及作物产量对该灌排技术的响应研究,以期为新疆盐碱地农业可持续发展提供理论依据和技术指导。     

毛白杨无性系木材基本密度遗传变异研究(英文)

对河北、河南、山东、甘肃的４块毛白杨无性系测定林中约１００个无性系进行了木材基本密度的测定，对木材基本密度在地点和无性系间的遗传变异进行了研究．结果表明：毛白杨无性系的木材基本密度平均值为０．４３６３ｇ／ｃｍ３，木材基本密度在地点和无性系间均存在极显著差异．木材基本密度的无性系重复力为０．８２～０．９１，属于受遗传控制较强的材性性状．对生长和材性的相关研究表明，木材基本密度与生长性状（包括胸径、树高和材积）一般存在显著的负相关，但不同地点上表现为不同的正或负的相关关系     

曹州木瓜果实香气物质的研究

利用顶空固相微萃取法提取芳香物质,气相色谱-质谱联机定性定量分析,测定曹州木瓜狮子头、剩花、豆青和玉兰的挥发性物质,并对木瓜不同品种的香气组分进行分析探讨.共测定出145种香气组分,曹州木瓜狮子头、剩花、豆青和玉兰分别为70、70、69和70种,各自占总峰面积的99.88%、99.7%、99.97%、99.37%,以醇类、醛类、烯烷类、酮类、酯类、杂环类等为主,其中又以C6醇(如1-己醇、2,4-己二烯-1-醇、反式-2-甲基-环戊醇等)、C6醛[(E)-2-己烯醛、2-己烯醛、E,E-2,4-己二烯醛等]居多.结果表明:狮子头、剩花、豆青和玉兰不同品种香气组分存在一定的差异,但大部分主要组分相同;主要组分含量差异是曹州木瓜不同品种间香气不同的主要原因.     

植物生长条件下土壤水分与养分资源动态分布的模拟研究

借助根系功能-结构机理模型,在动态描述根系空间生长发育的基础上,采用根系空间吸水速率函数耦合三维土壤水动力学模型,描述根系吸收条件下土壤水分的时空分布;以根个体为中心,采用扩散方式,描述了由于根系吸收土壤中磷元素而引起的土壤中有效磷分布的动态变化。通过小麦苗期根系结构与生物量的动态观测试验,获取了根系生长参数,利用文献资料获取了土壤水分运移参数、土壤有效磷扩散参数。在设定的灌溉与蒸散情景下,模拟了根长密度与根系吸收速率在三维空间的动态分布、根系吸水条件下土壤水分三维时空变化以及根系吸收磷元素的条件下土壤中有效磷的亏缺区域变化过程。模拟结果显示,根系-土壤系统能够实时地模拟根系生长下土壤环境资源的动态变化。