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51.
Lian-Ben Chang Chih-Jen Chou Jia-Shian Shiu Po-An Tu Shi-Xuan Gao Shao-Yu Peng Shinn-Chih Wu 《Tropical animal health and production》2017,49(6):1157-1162
Our aim was to investigate insemination techniques in order to improve pregnancy rates of artificial insemination (AI) using sex-sorted semen (sexed AI) in cattle in tropical and subtropical (T/ST) regions. In T/ST regions, the pregnancy rates by sexed AI are reportedly the lowest in the hottest months of the year, with less than 15% in cows and 35–40% in heifers (PMID 24048822). We compared sexed AI by depositing the semen into the uterine body (UB-AI, n = 12) versus the unilateral uterine horn (UUH-AI, n = 14) of pre-ovulation heifers. The ovary and follicle were assessed by rectal ultrasound before AI. After insemination, pregnancy was determined by ultrasound at approximately 40 days and approximately 70 days. In the present study, we demonstrated that high pregnancy rates (>70%) by sexed AI in the hottest season in a subtropical region such as Taiwan can be achieved when heifers with pre-ovulation follicles are used. The overall pregnancy rates were 54% higher in the UUH-AI (71%) group than in the UB-AI (42%) group (P = 0.06), examined on approximately 40 days post-sexed AI. Surprisingly, however, the pregnancy outcome appeared to be higher in the hot season (62%) than in the cool season (46%) although this difference was not statistically significant. Based on the present study, we recommend that cattle breeders perform UUH-AI using sex-sorted semen for heifers with pre-ovulation follicles in order to achieve satisfactory pregnancy outcome in the hot seasons in T/ST regions. 相似文献
52.
Maddur MS Mohan MS Gajendragad MR Kishore S Gopalakrishna S Singh N 《Veterinary research communications》2009,33(2):103-109
Humoral and mucosal (secretory antibody)immune response to FMDV type Asia 1 in cattle was analyzed after vaccination and infection
using virus neutralizing test (VNT). Vaccination (1/16th the usual dose) failed to protect cattle from generalized clinical
disease following experimental FMDV Asia 1 infection. Our results showed that infection induced higher and prolonged serum
antibody titres indicating antigen mass is important for optimal immune response. Experimental FMDV infection induced significant
secretory antibody (mucosal) response in cattle. Though, there was no difference in the serum antibody response between the
cattle that developed generalized infection (unprotected) and those with only localized infection (protected), secretory antibody
response differed, wherein the unprotected cattle had higher secretory response than protected cattle. Thus, FMDV Asia 1 infection
stimulates a similar serum antibody response and a unique secretory antibody response among the infected cattle.
An erratum to this article can be found at 相似文献
53.
Ararsa Duguma Shubisa Abera Wubishet Zewdie Dinaol Belina Godana Haro 《Tropical animal health and production》2017,49(3):445-450
A cross-sectional study was carried out from April 2015 to June 2016 to estimate the status of bovine tuberculosis (BTB), assessment of community’s current knowledge, and zoonotic importance on this disease in Borana zone, southern Ethiopia. In this study, comparative intradermal tuberculin (CIDT) test, structured questionnaires, and retrospective data were used, while the result indicated 3.8% prevalence at individual animal level with 5.6% (31/554) of doubtful reactors. Among related risk factors included, old animals were significantly infected by BTB than young one (χ 2?=?32.005, P?=?0.001). Parity number again showed significant difference (χ 2?=?29.163, P?=?0.001) where animals with many parity were more reactive to conducted test than few parity numbers. Animals born in the breeding center managed under semi-intensive production system were more infected (χ 2?=?10.795, P?=?0.029) than those brought from outside of the center. Questionnaire survey in this study indicated that out of 130 interviewed respondents, only 30% pastoralists knew what BTB mean; whereas the level of individual knowledge from interviewed showed about 72.3% of respondents had poor understanding of BTB and only about 11.5% of them knew its zoonotic importance. Meat eating habit of communities in the area were culturally inhabited to eat cooked meat and only 12.3% (16/130) of respondents gave response on habit of eating both raw and cooked meat. Milk drinking habit of pastoralist in the area showed about 79.2% drunk raw milk and the rest 20.8% used both raw and boiled milk. A retrospective data from Yabello Hospital indicated that current prevalence of human TB as 38.79% and showing the disease was highly increasing from year to year in the study area. This implies a great importance of human tuberculosis and its future concern in Borana zone. From this, there should be detail awareness of communities on BTB, its zoonotic importance, and the need of further investigation to develop control and prevention strategies according to the pastoral settings. 相似文献
54.
Cláudio Eduardo Silva Freitas Eduardo Robson Duarte Dorismar David Alves Isabel Martinele Marta D’Agosto Franciane Cedrola Angélica Alves de Moura Freitas Franklin Delano dos Santos Soares Makenzi Beltran 《Tropical animal health and production》2017,49(4):807-812
A ciliate protozoa suppression can reduce methane production increasing the energy efficiency utilization by ruminants. The physicochemical characteristics of rumen fluid and the profile of the rumen protozoa populations were evaluated for sheep fed banana leaf hay in replacement of the Cynodon dactylon cv. vaqueiro hay. A total of 30 male sheep were raised in intensive system during 15 days of adaptation and 63 days of experimental period. The animals were distributed in a completely randomized design that included six replicates of five treatments with replacement levels (0, 25, 50, 75, and 100%) of the grass vaquero for the banana leaf hay. Samples of fluid were collected directly from the rumen with sterile catheters. Color, odor, viscosity, and the methylene blue reduction potential (MBRP) were evaluated and pH estimated using a digital potentiometer. After decimal dilutions, counts of genus protozoa were performed in Sedgewick Rafter chambers. The averages of pH, MBRP, color, odor, and viscosity were not influenced by the inclusion of the banana leaf hay. However, the total number of protozoa and Entodinium spp. population significantly decreased at 75 and 100% inclusions of banana leaf hay as roughage. 相似文献
55.
U.S Salisu C.A Kudi J.O.O. Bale M Babashani B.Y Kaltungo S.N.A Saidu A Asambe A.Y Baba 《Tropical animal health and production》2017,49(5):1041-1046
A cross-sectional study was carried out to determine the status of Brucella infection in one-humped (Dromedary) camels in the North and Central senatorial districts of Katsina State, Nigeria. Nine hundred and eighty serum samples from live and slaughtered camels were tested. Modified Rose Bengal plate test (RBPT) and serum agglutination test (SAT) with ethylenediaminetetraacetic acid, (EDTA) were used as screening and standard tests, respectively. The prevalence of Brucella antibodies were 110 (11.2%) and 103 (10.5%) for RBPT and SAT, respectively. Of the 472 and 508 serum samples tested from the herds and abattoirs, respectively, 63 (13.3%) and 47 (9.3%) were positive by RBPT while 62 (13.1%) and 41 (8.1%) were positive by SAT, respectively. Based on the results, it was concluded that Brucella antibodies were present in camels in the study area. Poor management practices and mixing of camels with other species of livestock as well as unrestricted movement of camels were proposed to be the reasons for the prevalence of the disease in the study area. In view of the public health importance of the disease, it is recommended that there is the need to develop a strategic plan to decrease spread of brucellosis in the study area. 相似文献
56.
Gerardo Ordaz-Ochoa Aureliano Juárez-Caratachea Rosa Elena Pérez-Sánchez Rafael María Román-Bravo Ruy Ortiz-Rodríguez 《Tropical animal health and production》2017,49(5):1025-1033
The effect of spineless cactus intake (Opuntia ficus-indica) on blood glucose (BG) levels in lactating sows and its impact on daily and total feed intake (dFI?1 and TFI, respectively), body weight loss (BWL), and weaning-estrus interval length (WEI) were evaluated. Thirty-four hybrid (Yorkshire × Landrace × Pietrain) sows in lactation phase were used. Sows were divided into two groups: G1 (n = 17) where they received commercial feed and G2 (n = 17) provided with commercial feed plus an average of 2.0 ± 0.5 kg spineless cactus, based on a sow’s body weight. The variables evaluated were BG, dFI?1, TFI, BWL, and WEI. Statistical analysis was performed by using a fixed and mixed model methodology, under a repeated measurements experiment. Group effects were found on all analyzed variables (P < 0.05). The BG was lower in G2 (55.2 and 64.5 mg/dL pre- and post-prandial, respectively), compared to that in G1 (70.9 and 80.1 mg/dL pre- and post-prandial, respectively) (P < 0.05). G2 showed better performance than G1 for dFI?1, BWL, and WEI (P < 0.05) whose averages were 5.5 ± 1.8 kg, 7.4 ± 4.5%, and 5.3 ± 1.2 days, respectively. Averages for these variables in G1 were 4.7 ± 1.5 kg, 16.8 ± 4.6%, and 6.1 ± 1.6 days, respectively. Intake of spineless cactus reduced BG levels in lactating sows, generating greater dFI?1, lower BWL at the end of lactation, and a lower WEI. 相似文献
57.
In order to describe the normal bacterial flora in vaginas of Criollo Limonero cows, 51 healthy multiparous cows, at least
90-day postpartum, were selected. Duplicated swabs (N = 102) were taken from the vaginal fornix of cows to perform aerobic and anaerobic cultures as well as conventional biochemical
tests. Out of 102 swabs, bacterial growth was obtained in 55 (53.9%) while the remaining 47 (46.1%) did not exhibited any
bacterial growth. Of the 55 bacterial growths, 23 (41.8%) were aerobic whereas 32 (58.1%) were anaerobic. Likewise, 29 (52.72%)
of bacterial growths were pure and 26 (47.27%) were mixed. Under both aerobic and anaerobic conditions, Gram positive bacteria
were predominant (81.82% and 73.08%, respectively) over Gram negative bacteria (18.18% and 26.92%, respectively). Isolated
bacteria were Arcanobacterium pyogenes (22.92%), Staphylococcus aureus (15.63%), Staphylococcus coagulase negative (17.71%), Erysipelothrix rhusiopathiae (6.25%), Bacteroides spp. (13.54%), and Peptostreptococcus spp. (7.29%). In conclusion, normal vaginal bacterial flora of Criollo Limonero cows was predominantly Gram positive and included
A. pyogenes, S. aureus, coagulase negative Staphylococcus, E. rhusiopathiae, Bacteroides spp., and Peptostreptococcus spp. In Criollo Limonero cattle, adaptive aspects such as development of humoral and physical mechanisms for defense, and bacterial
adaptation to host deserve research attention. 相似文献
58.
59.
Hiskett EK Suwitheechon OU Lindbloom-Hawley S Boyle DL Schermerhorn T 《Veterinary research communications》2009,33(3):227-240
In most mammals, glucokinase (GK) acts as a hepatic “glucose sensor” that permits hepatic metabolism to respond appropriately
to changes in plasma glucose concentrations. GK activity is potently regulated by the glucokinase regulatory protein (GKRP),
which is encoded by the GCKR gene. GKRP binds GK in the nucleus and inhibits its activity. GK becomes active when it is released from GKRP and translocates
to the cytosol. Low glucokinase (GK) activity is reported to be a principal feature of feline hepatic carbohydrate metabolism
but the molecular pathways that regulate GK activity are not known. This study examined the hypothesis that species-specific
differences in GKRP expression parallel the low GK activity observed in feline liver. Hepatic GKRP expression was examined
using RT-PCR, immunoblot, and confocal immunomicroscopy. The results show that the GCKR gene is present in the feline genome but GCKR mRNA and the GKRP protein were absent in feline liver. The lack of GKRP expression in feline liver indicates that the low
GK activity cannot be the result of GKRP-mediated inhibition of the GK enzyme. However, the absence of the permissive effects
of GCKR expression on GK expression and activity may contribute to reduced GK enzyme activity in feline liver. The study results
show that the cat is a natural model for GCKR knockout and may be useful to study regulation of GCKR expression and its role in hepatic glucose-sensing and carbohydrate metabolism. 相似文献
60.