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31.
Adherence of Actinobacillus pleuropneumoniae serotype 1 to swine buccal epithelial cells involves fibronectin 下载免费PDF全文
Roberto Hamer-Barrera Delfino Godínez V. Idalia Enríquez Sergio Vaca-Pacheco Rodrigo Martínez-Zú?iga Patricia Talamás-Rohana Francisco Suárez-Güemez Mireya de la Garza 《Canadian journal of veterinary research》2004,68(1):33-41
The swine pathogen Actinobacillus pleuropneumoniae serotype 1 was investigated for its ability to adhere to swine, rat, and human buccal epithelial cells (BEC). The highest number of bacteria adhered was to swine BEC. This binding ability was affected by heating, extreme pH, treatment with sodium dodecyl sulfate, ethylenediamine tetraacetate, or periodate, and proteolysis, suggesting that cell-surface glycoproteins participate in adherence and that adherence is based mostly on ionic interactions. Mannose and swine fibronectin may play a direct role in this interaction. Convalescent-phase serum from naturally infected pigs inhibited the adhesion. There was a correlation between bacterial pathogenicity as well as host specificity and the capacity for adherence to swine BEC. Adhesion to swine BEC provides a convenient method to study in vitro the adherence of A. pleuropneumoniae and other pathogens of the pig respiratory tract. 相似文献
32.
Kun?Zong Jian?Huang Kazuhide?Nara Yahua?Chen Zhenguo?Shen Chunlan?LianEmail author 《Journal of Forest Research》2015,20(6):493-500
To advance our understanding of the effects of inoculation with ectomycorrhizal fungi (EMF) on seedling colonization in mine wastelands, we conducted a field experiment in a copper tailing. Six-month-old seedlings of Japanese red pine (Pinus densiflora) and oak (Quercus variabilis) separately inoculated with three EMF species (Pisolithus sp., Cenococcum geophilum, Laccaria laccata) were transplanted to the copper tailing. The survival rates of tree seedlings were monitored monthly, and growth (biomass and height), contents of nutrients and heavy metals (K, P, Ca, Mg, Cu, Zn), and mycorrhizal infection rates of seedlings were determined 6 months after planting. Oak seedlings exhibited higher survival rates than pine seedlings after 6 months of growth on the tailing. EMF inoculations of pine seedlings significantly enhanced their survival, growth, and nutrient uptake. In contrast, EMF inoculations of oak seedlings improved growth only in terms of biomass. Additionally, EMF inoculation caused pine seedlings to accumulate more Cu and Zn in roots compared to non-inoculated seedlings, whereas inoculation inhibited the accumulation of heavy metals in shoots. However, similar results were not observed in oak seedlings. Observations of roots indicated that the rates of mycorrhizal infection of both tree species had dramatically declined at harvest time. In conclusion, ectomycorrhizal symbioses can improve the survival and performance of pine seedlings in mine tailings. The present study provided direct evidence of the importance of EMF inoculation of seedlings to the reforestation of mine wastelands. 相似文献
33.
G Flachowsky H Wilk H J L?hnert W I Ochrimenko D Geinitz A Hennig 《Archives of Animal Nutrition》1990,40(1-2):85-100
Five experiments with 18 to 36 male calves each of the black and white dairy cattle breed (age: 14-21 days, initial live weight: approximately 45 kg per animal) were carried out in order to investigate the influence of various vitamin A supply (0-80,000 IU per 100 kg LW and day) on dry matter intake and weight gain as well as the vitamin A status of liver and blood plasma over 84 days. The calves consumed a diet free of carotene and vitamin A consisting of milk replacer, concentrate and chopped wheat straw. The calves were fed in three experiments for a longer time in order to observe the further vitamin A depletion. Nine animals consumed an unsupplemented ration, nine other one got 10,000 IU vitamin A per 100 kg LW and day. Biopsies of liver and plasma samples were taken from 4 animals per group every four weeks. The various vitamin A supplementation did not significantly influence the dry matter intake (Mean: 1.67; 1.48 to 1.80 kg DM per animal and day) and the weight gain of calves (Mean: 702, 599 to 770 g per animal and day). First vitamin A deficiency symptoms (reduced feed intake, decreased weight gain, diarrhoea etc.) were observed in animals of unsupplemented group after 100 days of experiments. After 84 days the vitamin A concentration of liver of animals of unsupplemented groups decreased to 1.3-32.2% compared with the begin of experiments (60.6-155.7 mumol/kg fresh matter). Up to 51% of initial concentration were found when 10,000 IU vitamin A per 100 kg LW and day were fed. About 25,000 IU vitamin A per 100 kg LW and day were required in order to keep the initial level of vitamin A concentration of liver. The plasma vitamin A concentration is unsuitable for estimation of vitamin A status of calves. The concentration of vitamin A of liver and plasma amounted to 114 mumol per kg and 0.25 mumol per litre at the begin of experiments. The vitamin A concentration of liver of unsupplemented group decreased to 20 mumol per kg, that of plasma increased to 0.28 mumol per 1 at the end. A strong vitamin A deficiency (liver concentration: less than 10 mumol/kg) may cause a decrease of vitamin A concentration of blood. 相似文献
34.
We derived an allometric model of the height–diameter curve for even-aged pure stands, which was a modification of the earlier
model proposed by Inoue (2000a). An individual-dependent allometric equation was used as the height–diameter curve. Using
the discriminant analysis method, all trees composed of a stand were stratified into upper and lower trees. It was assumed
that both relationships between mean tree height H
m
and upper tree height H
u
and between mean DBH D
m
and mean DBH of upper trees D
u
could be described by the time-dependent allometric power equations. The height–diameter curve showed an average relationship
between tree height and DBH of a given stand at a given time, and hence it could be assumed that the height–diameter curve
contained two points (D
m
, H
m
) and (D
u
, H
u
). With these assumptions, we derived an allometirc model of height–diameter curve, which allowed the coefficients of the
curve to be estimated from mean tree height and mean DBH. The proposed model was fitted to Japanese cedar (Cryptomeria japonica D. Don) data. The error ratio of the allometric model ranged from 2.254% to 13.412% (mean = 6.785%), which was significantly
smaller than that of the earlier model. When the error of mean tree height was ±1.0 m or less, the effect of the error of
mean tree height on the error ratio was comparatively small. This suggested that the error of ±1.0 m in mean tree height could
be accepted in the estimation of height–diameter curve using the allometric model. These features enable us to combine the
allometric model with Hirata’s vertical angle-count sampling or growth models. In conclusion, the allometric model would be
one of the most practical and convenient approaches for estimating the height–diameter relationship of even-aged pure stands. 相似文献
35.
Antti Oksanen Kjetil ?sbakk Marja Raekallio Mauri Nieminen 《Acta veterinaria Scandinavica》2014,56(1)
Background
Overwintering (breeding) reindeer (Rangifer tarandus tarandus) are commonly treated with ivermectin against parasitic infestations once yearly in autumn-winter roundups. The only preparations registered to reindeer are those for subcutaneous injection. However, also oral extra-label ivermectin administration is used. Twenty-six, 8-month-old reindeer calves were randomly allocated into three groups. Group 1 (n = 9) received oral ivermectin mixture (Ivomec® vet mixt. 0.8 mg/ml, oral ovine liquid drench formulation), Group 2 (n = 9) oral ivermectin paste (Ivomec® vet 18.7 mg/g equine paste), and Group 3 (n = 8) subcutaneous injection of ivermectin (Ivomec® 10 mg/ml vet inj.), each group at a dose of 200 μg/kg body weight. Blood samples were collected at treatment and at days 1, 2, 3, 6, 9 and 16 post treatment. Plasma concentrations of ivermectin were determined by high-pressure liquid chromatography (HPLC) with fluorescence detection.Results
The peak plasma concentration (Cmax) was reached by 2 days after each treatment. The Cmax and Area Under Curve (AUC) differed significantly between the groups: Cmax was 30.2 ± 3.9, 14.9 ± 5.7 and 63.1 ± 13.1 ng/ml, and AUC∞ was 2881 ± 462, 1299 ± 342 and 6718 ± 1620 ng*h/ml for groups 1, 2 and 3, respectively (mean ± standard deviation).Conclusions
The differences in plasma concentrations of ivermectin are concomitant with earlier observed differences in antiparasitic efficacy, which discounts the use of the equine paste in reindeer in favour of the oral ovine liquid drench formulation, or preferably, the reindeer-registered subcutaneous injection formulation. 相似文献36.
Shinpei?WadaEmail author Chutima?Hanjavanit Osamu?Kurata Kishio?Hatai 《Fisheries Science》2005,71(3):682-684
37.
38.
María?C.?Mateo-SánchezEmail author Aitor?Gastón Carlos?Ciudad Juan?I.?García-Vi?as Jorge?Cuevas César?López-Leiva Alfredo?Fernández-Landa Nur?Algeet-Abarquero Miguel?Marchamalo Marie-Josée?Fortin Santiago?Saura 《Landscape Ecology》2016,31(6):1261-1276
Context
Multi-scale approaches to habitat modeling have been shown to provide more accurate understanding and predictions of species-habitat associations. It remains however unexplored how spatial and temporal variations in habitat use may affect multi-scale habitat modeling.Objectives
We aimed at assessing how seasonal and temporal differences in species habitat use and distribution impact operational scales, variable influence, habitat suitability spatial patterns, and performance of multi-scale models.Methods
We evaluated the environmental factors driving brown bear habitat relationships in the Cantabrian Range (Spain) based on species presence records (ground observations) for the period 2000–2010, LiDAR data on forest structure, and seasonal estimates of foraging resources. We separately developed multi-scale habitat models for (i) each season (spring, summer, fall and winter) (ii) two sub-periods with different population status: 2000–2004 (with brown bear distribution restricted to the main population nuclei) and 2005–2010 (with expanding bear population and range); and (iii) the entire 2000–2010 period.Results
Scales of effect remained considerably stable across seasonal and temporal variations, but not the influence of certain environmental variables. The predictive ability of multi-scale models was lower in the seasons or periods in which populations used larger areas and a broader variety of environmental conditions. Seasonal estimates of foraging resources, together with LiDAR data, appeared to improve the performance of multi-scale habitat models.Conclusions
We highlight that the understanding of multi-scale behavioral responses of species to spatial patterns that continually shift over time may be essential to unravel habitat relationships and produce reliable estimates of species distributions.39.
Kyung?Min?Yoo Seok?Won?Lee Jae?Ryoun?YounEmail author Do?Heum?Yoon Young?Eun?Cho Jae-Pil?Yu Hyung?Sang?Park 《Fibers and Polymers》2003,4(2):89-96
A vertebral cage is a hollow medical device which is used in spine surgery. By implanting the cage into the spine column,
it is possible to restore disc and relieve pressure on the nerve roots. Most cages have been made of titanium alloys but they
detract the biocompatibility. Currently PEEK (polyether ether ketone) is applied to various implants because it has good properties
like heat resistance, chemical resistance, strength, and especially biocompatibility. A new shape of vertebral cage is designed
and injection molding of PEEK is considered for production. Before injection molding of the cage, it is needed to evaluate
process conditions and properties of the final product. Variables affecting the shrinkage of the cage are considered, e.g.,
injection time, packing pressure, mold temperature, and melt temperature. By using the numerical simulation program, MOLDFLOW,
several cases are studied. Data files obtained by MOLDFLOW analysis are used for stress analysis with ABAQUS, and shrinkage
and residual stress fields are predicted. With these results, optimum process conditions are determined. 相似文献
40.
Sandrine?Rousseaux Alain?Hartmann Nadine?Rouard Guy?SoulasEmail author 《Biology and Fertility of Soils》2003,37(4):250-254
We have studied the structural effects of application to the soil of a potentially detrimental herbicide, 4,6-dinitroorthocresol (DNOC) by analysing amplified ribosomal DNA restriction analysis (ARDRA) and terminal restriction fragment length polymorphism (T-RFLP) signatures of 16S rDNA fragments of culturable bacterial communities isolated from diluted soil suspensions. This approach has the potential to reveal changes induced by stressing the soil microflora with DNOC. This paper shows that, whereas only few changes of the ARDRA and T-RFLP profiles result from ageing of the soil, treatment of the soil with DNOC induces major modifications of these profiles. Therefore, for the practical purpose of pesticide registration, ARDRA and T-RFLP analysis performed on the dominant culturable fraction of the soil bacteria, implemented using conventional gel electrophoresis, offers the means of a routine, simple and meaningful test for detecting some of the changes affecting the structure of the soil microflora in response to pesticide application. 相似文献