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991.
Masahito WATANABE Mirina KOBAYASHI Masaki NAGAYA Hitomi MATSUNARI Kazuaki NAKANO Miki MAEHARA Gota HAYASHIDA Shuko TAKAYANAGI Rieko SAKAI Kazuhiro UMEYAMA Nobuyuki WATANABE Masafumi ONODERA Hiroshi NAGASHIMA 《The Journal of reproduction and development》2015,61(3):169-177
Monomeric Plum (Plum), a far-red fluorescent protein with photostability and photopermeability, is potentially suitable for in vivo imaging and detection of fluorescence in body tissues. The aim of this study was to generate transgenic cloned pigs exhibiting systemic expression of Plum using somatic cell nuclear transfer (SCNT) technology. Nuclear donor cells for SCNT were obtained by introducing a Plum-expression vector driven by a combination of the cytomegalovirus early enhancer and chicken beta-actin promoter into porcine fetal fibroblasts (PFFs). The cleavage and blastocyst formation rates of reconstructed SCNT embryos were 81.0% (34/42) and 78.6% (33/42), respectively. At 36–37 days of gestation, three fetuses systemically expressing Plum were obtained from one recipient to which 103 SCNT embryos were transferred (3/103, 2.9%). For generation of offspring expressing Plum, rejuvenated PFFs were established from one cloned fetus and used as nuclear
donor cells. Four cloned offspring and one stillborn cloned offspring were produced from one recipient to which 117 SCNT embryos were transferred (5/117, 4.3%). All offspring exhibited high levels of Plum fluorescence in blood cells, such as lymphocytes, monocytes and granulocytes. In addition, the skin, heart, kidney, pancreas, liver and spleen also exhibited Plum expression. These observations demonstrated that transfer of the Plum gene did not interfere with the development of porcine SCNT embryos and resulted in the successful generation of transgenic cloned pigs that systemically expressed Plum. This is the first report of the generation and characterization of transgenic cloned pigs expressing the far-red fluorescent protein Plum. 相似文献
992.
Shoma MIKAWA Yasuhiro OHTA Noriyuki KAJI Md Shafiqul ISLAM Takahisa MURATA Hiroshi OZAKI Masatoshi HORI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1443-1449
Endotoxin causes gastrointestinal motility disorder. Aim of this study is to clarify
inhibitory mechanisms of lipopolysaccharide (LPS) on smooth muscle contraction in rat
ileum. Ileal tissues were isolated from control rat or from LPS-induced peritonitis model
rat. Treatment with LPS inhibited carbachol (CCh)-mediated contraction in a time-dependent
manner. Cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) genes were
also upregulated, but iNOS expression was preceded by a rising of COX-2. All subtypes of
prostaglandin E2 (PGE2) receptors (EP1-EP4) were expressed in ileum,
and PGE2 and selective EP2 or EP4 agonist inhibited CCh-mediated contraction.
Selective iNOS inhibitor did not reverse LPS-induced inhibition of contraction by CCh at 1
and 2 hr, but reduced the inhibitory action at 4 hr after the LPS treatment. COX-2
inhibitor reversed the inhibitory action by LPS in all exposure time. Finally, in ileal
tissues isolated from peritonitis model rat, iNOS expression was upregulated only at 4 hr
after LPS administration, resulting in enhanced inhibitory action of LPS against
CCh-induced contraction. In conclusion, LPS induces COX-2 to produce PGE2,
which initially activates EP2 and/or EP4 on smooth muscle cells to inhibit the
contractility in early phase of LPS exposure. Moreover, in late phase of LPS treatment,
iNOS is expressed to produce NO, which in turn inhibited the contraction by CCh. The
inhibitory cascade is similar in the ileum isolated from peritonitis model rat, indicating
time-dependent changes of inhibitory action by LPS on intestinal motility in
peritonitis. 相似文献
993.
Takashi KOZASA Yuri ABE Kazuya MITSUHASHI Tomokazu TAMURA Hiroshi AOKI Masatoshi ISHIMARU Shigeyuki NAKAMURA Masatoshi OKAMATSU Hiroshi KIDA Yoshihiro SAKODA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(5):511-518
The Exaltation of Newcastle disease virus (END) phenomenon is induced by the
inhibition of type I interferon in pestivirus-infected cells in vitro,
via proteasomal degradation of cellular interferon regulatory factor (IRF)-3 with the
property of the viral autoprotease protein Npro. Reportedly, the amino acid
residues in the zinc-binding TRASH motif of Npro determine the difference in
characteristics between END-phenomenon-positive (END+) and
END-phenomenon-negative (END−) classical swine fever viruses (CSFVs). However,
the basic mechanism underlying this function in bovine viral diarrhea virus (BVDV) has not
been elucidated from the genomic differences between END+ and END−
viruses using reverse genetics till date. In the present study, comparison of complete
genome sequences of a pair of END+ and END− viruses isolated from
the same virus stock revealed that there were only four amino acid substitutions (D136G,
I2623V, D3148G and D3502Y) between two viruses. Based on these differences, viruses with
and without mutations at these positions were generated using reverse genetics. The END
assay, measurements of induced type I interferon and IRF-3 detection in cells infected
with these viruses revealed that the aspartic acid at position 136 in the zinc-binding
TRASH motif of Npro was required to inhibit the production of type I interferon
via the degradation of cellular IRF-3, consistently with CSFV. 相似文献
994.
Takuma AOKI Hiroshi SUNAHARA Keisuke SUGIMOTO Tetsuro ITO Eiichi KANAI Yoko FUJII 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):487-491
Case 1 involved a 4-month-old intact male Somali cat in which peripheral pulmonary artery
stenosis (PPS) was recognized after a cardiac murmur remained following patent ductus
arteriosus ligation. Case 2, which involved a 1-year-old neutered male Norwegian Forest
cat, and Case 3, which involved a 6-month-old intact female American Curl cat, were
referred, because of cardiac murmurs. Grades III to IV/VI systolic heart murmurs were
auscultated at the left heart base in all 3 cats. All cases showed bilateral pulmonary
artery stenosis, although there were no associated clinical signs. In Cases 1 and 2, the
pressure gradient through the stenosis decreased after treatment with atenolol. 相似文献
995.
Akifumi OHTANI Masahito KUBO Hiroshi SHIMODA Kenji OHYA Tadashi IRIBE Daiki OHISHI Daiji ENDOH Tsutomu OMATSU Tetsuya MIZUTANI Hideto FUKUSHI Ken MAEDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):777-782
Chlamydia pecorum (designated 22–58) was isolated in 2010 in
HmLu-1 cells from the jejunum of a calf which died of necrotizing enterocolitis in
Yamaguchi Prefecture, Japan. Immunohistochemical staining identified C.
pecorum positive reactions in the jejunal villi. C. pecorum,
designated 24–100, was isolated from the feces of a calf with diarrhea in another farm in
Yamaguchi Prefecture in 2012. A significant increase in neutralizing antibody titers
against C. pecorum was confirmed in paired sera. Nucleotide sequence
identities of omp1 genes of the 2 isolates were 100%. The isolates were
genetically and antigenically more closely related to C. pecorum
Bo/Yokohama strain isolated from cattle with enteritis in Japan than to the other
prototype strains, Bo/Maeda isolated from cattle with pneumonia and Ov/IPA isolated from
sheep with polyarthritis. These results indicate that C. pecorum strains
similar to 22–58 and 24–100 might be endemic in Yamaguchi Prefecture and cause enteric
disease in cattle. 相似文献
996.
Takuma AOKI Hiroshi SUNAHARA Keisuke SUGIMOTO Tetsuro ITO Eiichi KANAI Yoko FUJII 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):331-336
Infective endocarditis (IE) in dogs with cardiac shunts has not been reported previously.
However, we encountered a dog with concurrent patent ductus arteriosus (PDA) and IE. The
dog was a 1-year-old, 13.9-kg female Border collie and presented with anorexia, weight
loss, pyrexia (40.4°C) and lameness. A continuous murmur with maximal intensity over the
left heart base (Levine 5/6) was detected on auscultation. Echocardiography revealed a PDA
and severe aortic stenosis (AS) caused by aortic-valve vegetative lesions.
Corynebacterium spp. and Bacillus subtilis were
isolated from blood cultures. The dog responded to aggressive antibiotic therapy, and the
PDA was subsequently surgically corrected. After a series of treatments, the dog showed
long-term improvement in clinical status. 相似文献
997.
998.
Yuko Emoto Katsuhiko Yoshizawa Kei Hamazaki Yuichi Kinoshita Michiko Yuki Takashi Yuri Hiroshi Kawashima Airo Tsubura 《Journal of toxicologic pathology》2015,28(1):11-20
Fatty acids and their derivatives play a role in the response to ocular disease. Our current study investigated the effects of dietary mead acid (MA, 5,8,11-eicosatrienoic acid) supplementation on N-methyl-N-nitrosourea (MNU)-induced cataract and retinal degeneration in Sprague-Dawley rats. Experiment 1 was designed to inhibit cataract formation, with the dams fed a 2.4% MA or basal (<0.01% MA) diet during lactational periods. On postnatal day 7, male pups received a single intraperitoneal (ip) injection of 50 mg/kg MNU or vehicle. Lens opacity and morphology were examined 7 and 14 days after the MNU injection. Experiment 2 was designed to inhibit retinal degeneration and was performed with female postweaning rats. In this experiment, dams were fed the 2.4% MA or basal diet during the lactational periods. Thereafter, the female pups were continuously fed the same diets during their postweaning periods. On postnatal day 21 (at weaning), pups received a single ip injection of 50 mg/kg MNU. Retinal morphology was examined 7 days after the MNU injection. In experiment 3, six-week-old female rats were fed the 2.4% MA or basal diet starting at one week before the MNU injection and were then continuously fed the same diets until sacrifice. Rats at 7 weeks of age were given a single ip injection of 40 mg/kg MNU, and the retina was then examined morphologically one week after the MNU injection. In experiment 1, mature cataract was found in all of the MNU-treated groups, with or without MA supplementation. In experiments 2 and 3, atrophy of both the peripheral and central outer retina occurred in all rats exposed to MNU, with or without MA supplementation, respectively. The severities of the cataracts and retinal atrophy in the rats were similar regardless of MA supplementation. Dietary mead acid, which is used as a substitute in essential fatty acid deficiency in the body, does not modify MNU-induced cataract and retinal degeneration in rat models. 相似文献
999.
Takuma Aoki Hiroo Madarame Keisuke Sugimoto Hiroshi Sunahara Yoko Fujii Eiichi Kanai Tetsuro Ito 《The Canadian veterinary journal. La revue veterinaire canadienne》2015,56(7):745-748
We report the case of a 4-year-old, castrated 4.2-kg Scottish fold cat with recurrent epistaxis that was unresponsive to medical therapy. Diathermocoagulation of the nasal mucosa with a diode laser controlled the epistaxis and there was no significant recurrence of epistaxis during 1 year of follow-up. 相似文献
1000.