全文获取类型
收费全文 | 1545篇 |
免费 | 57篇 |
国内免费 | 4篇 |
专业分类
林业 | 145篇 |
农学 | 44篇 |
基础科学 | 3篇 |
251篇 | |
综合类 | 57篇 |
农作物 | 80篇 |
水产渔业 | 166篇 |
畜牧兽医 | 732篇 |
园艺 | 17篇 |
植物保护 | 111篇 |
出版年
2022年 | 27篇 |
2021年 | 42篇 |
2020年 | 14篇 |
2019年 | 22篇 |
2018年 | 23篇 |
2017年 | 36篇 |
2016年 | 52篇 |
2015年 | 66篇 |
2014年 | 81篇 |
2013年 | 121篇 |
2012年 | 94篇 |
2011年 | 120篇 |
2010年 | 49篇 |
2009年 | 56篇 |
2008年 | 104篇 |
2007年 | 105篇 |
2006年 | 106篇 |
2005年 | 100篇 |
2004年 | 107篇 |
2003年 | 88篇 |
2002年 | 65篇 |
2001年 | 44篇 |
2000年 | 27篇 |
1999年 | 11篇 |
1998年 | 9篇 |
1997年 | 7篇 |
1996年 | 2篇 |
1995年 | 5篇 |
1994年 | 4篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1984年 | 3篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1977年 | 1篇 |
1974年 | 2篇 |
1973年 | 1篇 |
排序方式: 共有1606条查询结果,搜索用时 15 毫秒
51.
Kenji Kawashima Ken Katsuda Hiroshi Tsunemitsu 《Journal of veterinary diagnostic investigation》2007,19(1):60-68
To investigate the prevalence and features of postweaning multisystemic wasting syndrome (PMWS) in Japan, an epidemiological study was conducted in 692 weaned pigs with various clinical signs, commonly including wasting or weight loss, collected from 129 swine farms between 2000 and 2003. The presence of PMWS was diagnosed by the detection of characteristic histological lesions and moderate to large amounts of porcine circovirus type 2 (PCV2) antigen within the lesions in multiple lymphoid tissues. Postweaning multisystemic wasting syndrome was positive in 23.4% of pigs (162/692) over the course of the study, and occurred in 50.4% of the farms (65/129). Mortality in 30-120-day-old pigs in the farms positive for PMWS varied from 0.1 to 32.0%. No significant difference in mortality was seen between PMWS-positive and -negative farms (P = 0.1). However, mortality was significantly higher in the PMWS-positive farms where PMWS was diagnosed in more than 50% of the pigs examined compared to farms negative for PMWS (P = 0.02). These findings indicate that PMWS has spread widely in Japan. Moreover it may exist in variable forms in swine farms, including an epidemic form or a subtle endemic or sporadic form. A case-control study suggested that risk factors for the occurrence of PMWS include porcine reproductive and respiratory syndrome (PRRS) pneumonias and Mycoplasma hyorhinis infection. 相似文献
52.
Effects of amino nitrogen on fermentation parameters by mixed ruminal microbes when energy or nitrogen is limited 总被引:1,自引:0,他引:1
Hiroshi KAJIKAWA Kiyoshi TAJIMA Makoto MITSUMORI Akio TAKENAKA 《Animal Science Journal》2007,78(2):121-128
Ruminal microbes harvested from a ruminally fistulated cow were incubated in simple batch and semicontinuous cultures with NH3‐N or amino‐N on nitrogen‐ or energy‐excess diets in quantity (HN and LN diets, respectively, consisting of timothy hay plus soybean meal, or corn grain), based on evaluation with the National Research Council and Cornell Net Carbohydrate and Protein System models. In a batch culture experiment, supplementation with amino‐N promoted digestion and fermentation in the course of incubation (4–24 h) on both diets, but these effects mostly disappeared when the diets were sufficiently digested (at 48 h). In a semicontinuous culture experiment using Rusitec, no effect of amino‐N was exhibited after sufficient fermentation and digestion, but significant promotion of digestion was shown in the course of incubation on the HN diet, while no such effect was detected on the LN diet. The microbial yield for 24 h did not show a significant difference between the N sources of either of the two diets. These results suggest that the stimulatory effects of amino‐N are diminished when the diets are sufficiently digested after a long retention and incubation, and also that the effectiveness of amino‐N does not require a quantitatively energy‐excess status. 相似文献
53.
Natsuki KASUYA Itsuko WADA Mimune SHIMADA Hiroshi KAWAI Hisao ITABASHI 《Animal Science Journal》2007,78(3):275-280
The cell wall constituents of feces from three faunated and three defaunated (without ruminal ciliate protozoa) cattle fed on a Sudangrass hay and concentrate mixture (8:5) were analyzed. There was little difference in digestibility of dry matter between the faunated and defaunated cattle. Analysis of the fecal sugar residues revealed that the digestibilities of arabinose and galactose, derived from pectic and hemicellulosic substances located within the compound middle lamella, were higher in the defaunated cattle than the faunated cattle (P < 0.05), whereas the digestibilities of glucose and xylose, derived mainly from cellulose and xylan, were unchanged by the removal of protozoa. The digestibility of lignin was not different between the faunated and defaunated cattle, but those of mannose and p‐coumaric acid were lower in the defaunated than in the faunated animals (P < 0.05). The ratio of primary cell wall to secondary cell wall in fecal plant materials was lower for the defaunated than for the faunated cattle. The results in this study suggested that the defaunation enhanced the microbial degradation of the thin cell walls, but depressed the degradation of developed cell walls. 相似文献
54.
Kumiko Oka Akiko Okubo Motoichiro Kodama Hiroshi Otani 《Journal of General Plant Pathology》2006,72(3):152-158
In tomato plants, α-tomatine, a steroidal glycoalkaloid saponin, inhibits fungal growth. Tomato pathogens that produce host-specific
toxins, Alternaria alternata tomato pathotype causing Alternaria stem canker and Corynespora cassiicola causing Corynespora target spot, were investigated for sensitivity to α-tomatine. Although spore germination of A. alternata pathogenic and nonpathogenic to tomato and of C. cassiicola pathogenic to tomato was not affected by 0.1 mM α-tomatine, spore germination of C. cassiicola nonpathogenic to tomato was significantly inhibited. This result showed that A. alternata, regardless of its pathogenicity, and only the C. cassiicola pathogenic to tomato are resistant to α-tomatine. Germinating spores of A. alternata and C. cassiicola resistant to α-tomatine detoxified α-tomatine by degrading it to a less polar product. After inoculation of tomato leaves,
spores of A. alternata and C. cassiicola nonpathogenic to tomato germinated and formed appressoria, but did not form infection hyphae in host tissues. When a host-specific
toxin (CCT-toxin) produced by C. cassiicola pathogenic to tomato was added to nonpathogenic spores, colonization within leaves was observed in A. alternata, but not in C. cassiicola. On the other hand, when spores of C. cassiicola nonpathogenic to tomato were suspended in spore germination fluid of nonpathogenic A. alternata with α-tomatine detoxification activity, the fungus could be induced to colonize leaves in the presence of CCT-toxin. These
results indicate that A. alternata tomato pathotype and C. cassiicola pathogenic to tomato detoxify α-tomatine during infection and that this detoxification is essential for host colonization
by pathogens that produce host-specific toxins. 相似文献
55.
Daisuke Yamagishi Hajime Akamatsu Hiroshi Otani Motoichiro Kodama 《Journal of General Plant Pathology》2006,72(5):323-327
Host-specific AAL-toxins and mycotoxin fumonisins are structurally related and were originally isolated from the tomato pathotype
of Alternaria alternata and from Fusarium verticillioides, respectively. Previous reports on the production of fumonisin derivatives by the tomato pathotype suggested a possible involvement
in the pathogenicity of the pathogen. Here, we have evaluated the role of fumonisin in A. alternata–tomato interactions. The results indicate that highly pathogenic isolates of A. alternata tomato pathotype produce AAL-toxin as the sole toxin, strongly implicating it as a pathogenicity factor. The related compound,
fumonisin, is also toxigenic and has infection-inducing activity on susceptible tomato plants. 相似文献
56.
Kyoji Hagiwara Takahiko Higashi Kazunari Takahashi Naho Hara Hideyuki Aoki Naoyuki Miyazaki Qing-Yu Wang Yafeng Zhu Osamu Yatou Hiroshi Tanaka Toshihiro Omura 《Journal of General Plant Pathology》2006,72(3):186-189
Transencapsidation of the Rice gall dwarf virus (RGDV) inner core by the Rice dwarf virus (RDV) outer capsid P8 protein was examined in vitro and in planta. When RGDV core particles were incubated with an extract
from RDV P8-transgenic rice leaf tissue, RDV P8 encapsidated the RGDV core particles to form double-shelled virus-like particles
in vitro. In contrast, when RDV P8-transgenic rice plants were inoculated with RGDV, progeny RGDV particles contained RGDV
P8 but RDV P8 was not detectable in the virions. No significant differences were found in acquisition by the vector insects
and subsequent transmission rates between RGDV infecting nontransgenic rice plants and those infecting RDV P8-transgenic rice
plants. These results indicate that mechanisms of and/or requirements for interactions between P8 and the inner core particles
of phytoreoviruses differ between in vitro and in planta. 相似文献
57.
Treatment of cow-waste slurry by a microbial fuel cell and the properties of the treated slurry as a liquid manure 总被引:1,自引:0,他引:1
Hiroshi YOKOYAMA Hideyuki OHMORI Mitsuyoshi ISHIDA Miyoko WAKI Yasuo TANAKA 《Animal Science Journal》2006,77(6):634-638
Resource recycling and the proper treatment of animal waste to reduce its environmental impact are currently important issues for the livestock industry. A microbial fuel cell (MFC), a new type of bioreactor, is expected to play roles in both waste‐water purification and energy recovery. However, the generation of electricity from cow waste has not yet been examined. In this study, using an MFC, we examined the possibility of generating electricity from dairy‐cow waste slurry, and analyzed the properties of the treated slurry as liquid manure for resource recycling. The MFC treatment of the slurry generated electricity in a dose‐dependent manner, and the maximum power output by the MFC from a 1 g of chemical oxygen demand/L slurry was 0.34 mW/m2. After the MFC treatment, 84% of the biological oxygen demand in the slurry was removed and three essential fertilizer elements (nitrogen, phosphorus, and potassium) were retained at 84, 70, and 91% levels, respectively. The amount of ammonia nitrogen in the slurry, as an element of fast‐release fertilizer, was increased by 1.9‐fold. Although the treated slurry displayed properties that made it preferable as liquid manure, further studies to improve the electrical power output by the MFC are required for practical use. 相似文献
58.
We investigated the validity and efficiency of a survey using sight per unit effort (SPUE) of sika deer and shrub-layer decline
rank (SDR), which is an index of decline in the physical structure of a whole stand caused by sika deer, based on data collected
on a broad scale. This survey was to be used to manage a deer population in order to conserve a forest ecosystem. First, we
evaluated the spatial and temporal scales of deer density that are most appropriate for predicting decline in the status of
understory vegetation. The model with SPUE calculated in a buffer with a radius of 4.5 km using data for the past 4 years
was found to be the best. We showed that our knowledge of the relationship between deer density and status of shrub-layer
vegetation is improved by identifying the most suitable spatial and temporal scales of SPUE for predicting SDR. Next, we quantified
the effects of SPUE and environmental components on SDR in stands. We found that SPUE had the greatest effect on SDR among
all explanatory variables. Moreover, the area under the curve (AUC) was large in a model that only used SPUE (AUC = 0.718).
This result suggests that the variation in SDR among stands was explained well by SPUE regardless of differences in the forest
environment. Furthermore, we identified the effective values of SPUE for preventing shrub-layer vegetation from declining
through deer density control. We conclude that a management system based on SPUE and SDR is a simple and valid method for
managing deer populations in order to conserve forest ecosystems. 相似文献
59.
60.
We made several buckling tests of wooden columns with intermediate slenderness ratios () and examined the empirical formulas. On the basis of the examination, we formulated an equation for predicting the buckling stress () of an intermediate wooden column. Sitka spruce (Picea sitchensis Carr.) and buna (Japanese beech, Fagus crenata BI.) were used for the studies. A compressive load was applied on the specimen supported with pin ends, and the buckling stress was predicted by the tangent modulus theory and two empirical equations: those of Tetmajer and Newlin-Gahagan. The predicted - relations were compared with the test results, and the applicability of these predictions were examined. Based on the comparisons, we formulated an equation that can predict the - relations of materials with various stress-strain characters in the plastic strain range. 相似文献