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991.
Heat stress adversely affects wheat production in many regions of the world and is particularly detrimental during reproductive
development. The objective of this study was to identify novel quantitative trait loci (QTL) associated with improved heat
tolerance in wheat (Triticum aestivum L.) and to confirm previous QTL results. To accomplish this, a recombinant inbred line (RIL) population was subjected to
a three-day 38°C daytime heat stress treatment during early grain-filling. At maturity, a heat susceptibility index (HSI)
was calculated from the reduction of three main spike yield components; kernel number, total kernel weight, and single kernel
weight. The HSI, as well as temperature depression (TD) of the main spike and main flag leaf during heat stress were used
as phenotypic measures of heat tolerance. QTL analysis identified 14 QTL for HSI, with individual QTL explaining from 4.5
to 19.3% of the phenotypic variance. Seven of these QTL co-localized for both TD and HSI. At all seven loci, the allele for
a cooler flag leaf or spike temperature (up to 0.81°C) was associated with greater heat tolerance, indicated by a lower HSI.
In a comparison to previous QTL results in a RIL population utilizing the same source of heat tolerance, seven genome regions
for heat tolerance were consistently detected across populations. The genetic effect of combining three of these QTL, located
on chromosomes 1B, 5A, and 6D, demonstrate the potential benefit of selecting for multiple heat tolerance alleles simultaneously.
The genome regions identified in this study serve as potential target regions for fine-mapping and development of molecular
markers for more rapid development of heat tolerant germplasm. 相似文献
992.
Self-incompatibility (SI) is a widespread mechanism in flowering plants that promotes outbreeding and thereby increases genetic
diversity. Recognition specificity in Brassica is achieved by the interaction of the female determinant S-receptor kinase (SRK) and its ligand, the male determinant S-locus protein 11 (SP11). The interaction between SP11 and SRK triggers the signaling cascade in an S-haplotype-specific manner and results in the rejection of self-pollen, but the signal components involved are still not well
characterized. S haplotypes are widespread in self-compatible amphidiploid B. napus, and the interaction of heterozygous S haplotypes causes the loss of SI. This review highlights the recent advances made towards understanding the genetic analysis,
distribution, and evolution of S haplotypes, the signal factors, and the potential of SI in B. napus hybrid breeding program. 相似文献
993.
Beatriz Wiebke-Strohm Annette Droste Giancarlo Pasquali Marina Borges Osorio Lauro Bücker-Neto Luciane Maria Pereira Passaglia Marta Bencke Milena Schenkel Homrich Márcia Margis-Pinheiro Maria Helena Bodanese-Zanettini 《Euphytica》2011,177(3):343-354
An Agrobacterium-mediated transformation procedure for soybean [Glycine max L. Merrill] proliferating somatic embryos is here described. The Agrobacterium tumefaciens LBA4404 strain harboring pTOK233, pCAMBIA1390-olp or pH7WG2Dwrky plasmids was used to mediate gene transfer into the plant genome. Prior to Agrobacterium inoculation, proliferative soybean embryogenic clusters were microwounded by DNA-free tungsten particle bombardment. Three
independent transformation experiments were performed. In Experiment I, 26 transgenic plants were obtained from a unique clone
of cv Bragg, while 580 plants were recovered from 105 clones of cv IAS5. In Experiment II, a single hygromycin-resistant clone
of cv BRSMG68 Vencedora was recovered and gave rise to five plants. In Experiment III, 19 plants of cv Bragg and 48 plants
of IAS5 were recovered, representing five and 14 independent transformation events, respectively. PCR and Southern analyses
confirmed the transgenes’ integration into plant genomes. Transgenic plants were fertile. They flowered, set pods and seeds.
Transgene segregation in two T1 progenies fits the Mendelian pattern (3:1 transgenic:non-transgenic plants). This is the first report of transgenic fertile
soybean plants obtained from somatic embryogenic tissues transformed by the system that combines DNA-free particle bombardment
and Agrobacterium. 相似文献
994.
Shelley Jansky 《Euphytica》2011,178(2):273-281
Valuable genetic diversity in diploid wild Solanum species can be accessed through crosses to haploids (2n = 2×) of the tetraploid cultivated potato, Solanum tuberosum. Haploid-wild species hybrids segregate for the ability to tuberize in the field. In addition, they vary in male fertility,
vine size, stolon length, and tuber size. In this study, three haploids were crossed with nine diploid wild Solanum species and 27 hybrid families were evaluated in the field for two years. The proportion of male fertile hybrid clones varied
depending on the wild species parent. A large effect of the female parent was detected for vine size, stolon length, tuber
size, percent tuberization, and percent plants selected for agronomic quality. An exceptional haploid (US-W4) was identified
for the production of agronomically desirable haploid-wild species hybrids. In hybrids derived from US-W4, differences among
wild species parents were observed for agronomic quality. Superior hybrids were produced by S. berthaultii and S. microdontum. Reciprocal crosses were evaluated for a subset of families. When the wild species was used as the female parent, male fertility
was restored, but tuberization and tuber size were reduced. Careful selection of both haploid and wild species parents can
result in a large proportion of fertile, agronomically desirable hybrid offspring. 相似文献
995.
A. M. Anithakumari Oene Dolstra Ben Vosman Richard G. F. Visser C. Gerard van der Linden 《Euphytica》2011,181(3):357-369
Drought stress is a major abiotic constraint limiting crop production worldwide. Screening for drought tolerance and the traits
that enhance drought tolerance is not straightforward in large mapping populations. In this study, we investigated the possibility
of screening a mapping population in vitro for PEG-induced water deficit stress and recovery potential. We have measured several
shoot and root growth parameters or traits in the C × E diploid potato mapping population. Significant variation was observed
for genotype-specific responses to water deficit and recovery potential. Genetic variation and heritability estimates were
high to very high for the measured traits depending on growth conditions. In order to identify potato QTLs for drought tolerance
and recovery potential an SNP marker-rich integrated linkage map was used. A total of 23 QTLs were detected under control,
stress and recovery treatments explaining 10.3–22.4% of the variance for each phenotypic trait. Among these, 10 QTLs were
located on chromosome 2. Three QTLs involved in the important trait root to shoot ratio were identified on linkage groups
2, 3 and 8. These loci explained together 41.1% of the variance for this trait, and may be breeding targets for stress tolerance
and yield in the field as well. The SNP markers derived from EST sequences underlying these QTLs led to the identification
of putative candidate genes for further study in potato. This study constitutes the first knowledge of in vitro screening
of a mapping population for drought tolerance in potato. 相似文献
996.
Srinivasan Samineni Pooran M. Gaur Timothy D. Colmer L. Krishnamurthy Vincent Vadez Kadambot H. M. Siddique 《Euphytica》2011,182(1):73-86
Chickpea (Cicer arietinum L.) is known to be salt-sensitive and in many regions of the world its yields are restricted by salinity. Recent identification
of large variation in chickpea yield under salinity, if genetically controlled, offers an opportunity to develop cultivars
with improved salt tolerance. Two chickpea land races, ICC 6263 (salt sensitive) and ICC 1431 (salt tolerant), were inter-crossed
to study gene action involved in different agronomic traits under saline and control conditions. The generation mean analysis
in six populations, viz. P1, P2, F1, F2, BC1P1 and BC1P2, revealed significant gene interactions for days to flowering, days to maturity, and stem Na and K concentrations in control
and saline treatments, as well as for 100-seed weight under salinity. Seed yield, pods per plant, seeds per plant, and stem
Cl concentration were controlled by additive effects under saline conditions. Broad-sense heritability values (>0.5) for most
traits were generally higher in saline than in control conditions, whereas the narrow-sense heritability values for yield
traits, and stem Na and K concentrations, were lower in saline than control conditions. The influence of the sensitive parent
was higher on the expression of different traits; the additive and dominant genes acted in opposite directions which led to
lower heritability estimates in early generations. These results indicate that selection for yield under salinity would be
more effective in later filial generations after gene fixation. 相似文献
997.
Aida Z. Kebede Baldev S. Dhillon Wolfgang Schipprack Jose L. Araus Marianne Bänziger Kassa Semagn Gregorio Alvarado Albrecht E. Melchinger 《Euphytica》2011,180(2):219-226
For in vivo production of doubled haploid (DH) lines in maize, the rate of haploid induction is of crucial importance. Maternal
haploid induction depends primarily on the inducer used as a pollinator. However, the source germplasm used as a maternal
parent and the environmental conditions for induction may also influence haploid induction and these aspects have not been
examined in tropical maize so far. The objectives of our study were to (i) monitor the variation for haploid induction rate
(HIR) among diverse source germplasm in tropical maize, (ii) determine the relative importance of general (GCA) and specific
(SCA) combining abilities for HIR, and (iii) investigate the influence of summer and winter seasons and genotype × season
interactions on this trait. Ten inbreds were mated in a half diallel design. The resulting 45 F1 single crosses were pollinated with the haploid inducer hybrid RWS × UH400 during the summer 2008 and winter 2009 seasons
in a lowland tropical environment in Mexico. HIR of the single crosses averaged over seasons ranged from 2.90 to 9.66% with
an overall mean of 6.74%. Mean HIR was significantly (P < 0.01) higher during the winter (7.37%) than summer season (6.11%). Significant (P < 0.01) variation was observed due to GCA effects of parental inbreds of single crosses but not for SCA, GCA × season and
SCA × season interactions. Our study underpins that a higher HIR in tropical maize can be obtained by selecting appropriate
source germplasm and undertaking pollination under favorable environmental conditions. 相似文献
998.
Hela Chikh-Rouhou Rafael González-Torres Ali Oumouloud José M. Alvarez 《Euphytica》2011,182(2):177-186
The resistance to Fusarium oxysporum f.sp. melonis (Fom) race 1.2 has been studied in melons, such as the Portuguese accession ‘BG-5384’ and in the Japanese ‘Shiro Uri Okayama’,
‘Kogane Nashi Makuwa’, and ‘C-211’, since a good characterization of the resistance is necessary before its introgression
into commercial varieties. These four melon accessions showed a high level of resistance to races 0, 1, and 2 of Fom, indicating that the partial resistance to the race 1.2 previously detected may not have been race specific. To determine
the mode of inheritance of the resistance to Fom race 1.2, the F1, F2, BCPR, and BCPS generations from the crosses between the four resistant accessions above and ‘Piel de Sapo’, a Fom race 1.2 susceptible melon, were developed. They were subsequently inoculated with two Fom isolates, one from the pathotype 1.2Y and the other from the pathotype 1.2W. The area under the disease progress curve was
determined for each inoculated plant, and the data were analyzed. We show that the resistance seen in these accessions is
polygenically inherited with a complex genetic control because many epistatic interactions were detected. The three epistatic
effects; additivity × additivity, dominance × dominance, and dominance × additivity are present and significant, with differing
magnitudes from one cross to the next. The relatively low heritabilities, and these epistatic effects make difficult the improvement
of the resistance, from these sources, through a standard selection procedure. 相似文献
999.
Reciprocal differences, mostly caused by cytoplasmic effects, are frequently observed in interspecific hybrids. Previously,
we found that crosses onto Solanum demissum were much successful with the pollen of interspecific hybrids between S. tuberosum as female and S. demissum as male (TD hybrids) than the reciprocal ones (DT hybrids). To elucidate this reciprocally different crossability, we analyzed
the pollen DNA of TD and DT using methylation-sensitive amplified polymorphism (MSAP) analysis. Using 126 primer combinations,
MSAP analysis revealed 57 different bands between bulked pollen DNA samples of TD and DT. Individual examination of 16 TD
and 9 DT plants disclosed eight bands uniformly different between TD and DT. Their sequencing results revealed two pairs of
bands to be identical to each other, resulting in six distinct sequences. As expected, one band shared high homology with
chloroplast DNA, and another one with mitochondrial DNA. However, one band that was apparently different at DNA sequence level
and maternally transmitted from S. demissum, showed no homology with any known sequence. The remaining three bands were of DNA methylation level differences with no
or uncertain homology to known sequences. To our knowledge, this is the first report detecting reciprocal differences in DNA
sequence or DNA methylation other than those in cytoplasmic DNA. 相似文献
1000.
长枝木霉TICC鉴定及其生物学特性研究 总被引:2,自引:0,他引:2
从土壤中分离得到1株木霉T1CC,为明确其分类地位及其生物学特性,对该菌进行形态学鉴定和分子鉴定,并对其生长培养基、光照、温度、碳源、氮源、微量元素和pH等生物学特性进行研究.鉴定结果显示,该菌株为长枝木霉(Trichoderm longibrachiatum).生物学特性结果表明:菌株T1CC在PDA上生长量最大,查氏培养基和基本培养基上生长量最小;不同光照条件下,菌丝生长无明显差异,但在连续光照条件下可明显促进孢子产生;在15~40℃范围能够生长产孢,在35℃时菌丝生长最快,但产孢量在25~35℃最大;果糖最能促进菌丝生长,果糖、可溶性淀粉最有利于产孢;谷氨酸对菌丝生长和孢子产生都有促进作用;Cu最能促进菌丝生长,而zn、K和Mg则对菌丝生长有一定抑制作用,Cu最有利于产孢,zn对产孢明显抑制;在pH/值在2~10范围内均可生长,在pH为5时为生长最佳,在pH值3~7范围内最有利于产孢. 相似文献