Comparative study on responses of cattle and water buffalo (<Emphasis Type="Italic">Bubalus bubalis</Emphasis>) to experimental inoculation of <Emphasis Type="Italic">Brucella abortus</Emphasis> biovar 1 by the intraconjunctival route—a preliminary report

The preliminary study was conducted to assess the virulence of a strain of Brucella abortus (1969D) and to compare the susceptibility of water buffalo and cattle calves to infection by the intraconjunctival route. Seven of each cattle and water buffalo (Bubalus bubalis) calves aged 3–6 months were inoculated intraconjunctivally with counts ranging from 1.5 × 10⁷ to 1.7 × 10¹⁰ colony forming units of B. abortus. Animals were monitored over an 8-week period for clinical manifestations and serological and hematological evidence of infection. At slaughter, eight lymph nodes from each animal were sampled for bacteriological and histopathological assessments. Lymph nodes from three water buffalo (43%) and five cattle (71%) yielded B. abortus (P = 0.048). Parotid/prescapular lymph nodes were most sensitive in detecting B. abortus. Our data suggest that B. abortus strain 1969D may be used as challenge strain, and water buffalo appeared to have a lower susceptibility to B. abortus infection than cattle.     

Temporal changes in endogenous estrogens and expression of behaviors associated with estrus during the periovulaory period in Murrah buffaloes (<Emphasis Type="Italic">Bubalus bubalis</Emphasis>)

The objective of this study were (1) to establish the duration of behavioral estrus signs and timing of ovulation in Murrah buffaloes (n = 10) and (2) to determine relationship between behavioral estrus signs with change in plasma estrogen concentrations. Estrus and its behavioral signs were detected at hourly intervals by visual observations, per recta examination of genitalia and bull parading four times in a day for 30 minutes each. Among the behavioral signs of estrus, swollen vulva (80%) was the best indicator of estrus followed by excitement (70%). Among the duration of behavioral estrus signs the first and longest duration of estrus signs was swollen vulva which was seen upto 19.8 ± 0.8 h after onset of estrus. The mean total duration of estrus symptoms from appearance to disappearance of all the behavioral estrus symptoms was 23.5 ± 1.7 h. All the behavioral estrus symptoms were observed during the period of estrogen surge. Endocrine profile during the periestrus period showed that the mean peak concentrations of total estrogen 23.9 ± 3.9 pg/ml occurred at 8.8 ± 1.7 h after onset of estrus. The average number of estrus symptoms observed per animal during onset of spontaneous estrus was 5.7. Ovulation occurred after 37.4 ± 1.7 h after onset of estrus and 13.4 ± 1.0 h after end of total estrogen surge respectively. In conclusion, our results suggest that all signs of behavioral estrus occurred during the preovulatory rise in estrogens. The first sign of estrus to be observed was a swollen vulva and this symptom persisted the longest.     

Clinicopathological studies on facial eczema outbreak in sheep in Southwest Turkey

After very hot summer, 22 sheep from 5 different flocks consisting of approximately 150-200 animals each were diagnosed with facial eczema in September 2005, in southwest Turkey. Photophobia, corneal opacity, severe ulcers of the facial skin, especially localized around the eyes and mouth, and 3% mortality were the most prominent clinical symptoms. GGT levels of the animals were very high and varying between 261- 328 U/l. While the activities of ALT and total bilirubin were elevated and AST was normal in affected sheep. Total bilirubin level was higher than normal. Seven of the 22 sheep were euthanatized and necropsy was performed on all of these animals. Severe icterus, hepatomegaly, enlarged gallbladder, congestion of mesenteric vessels were the common necropsy findings. Histopathological changes of the liver included necrosis of the hepatocytes, cholangiohepatitis characterized by mononuclear inflammatory cell infiltrate in the portal area and mild to severe fibrosis around bile ducts. A diagnosis of sporidesmin toxicosis was made based on the histopathology of the livers, the elevation in liver enzymes, and the development of cutaneous lesions consistent with photosensitization and high spore counts in the ruminal contents. Surviving sheep were treated with procaine penicillin + dihidrostreptomycin sulfate, multivitamin complexes and flunixin meglumine. Additionally, zinc sulphate was also given at a dose of 6 gr per 100 lt drinking water for 28 days. All treated sheep recovered. Pasture spore counts were between 96,300- 267,500 spores/g grass.     

Effects of artemisinin in broiler chickens following chronic oral intake

Artemisinin has been used for centuries to treat malaria, intestinal tract helminthosis, diarrhea, and used as an antipyretic and sedative agent, but the usage in veterinary medicine is a new field. Recently, it has been used successfully to control experimental poultry coccidiosis. The present study aimed to determine the effects of different doses of artemisinin in broiler chickens with chronic usage. Sixty birds divided into one control and four treatment groups that fed rations mixed with artemisinin at doses of 17, 34, 68, and 136 ppm for 36 days. During the experiment, birds showed no clinical signs except anemia. In microscopic examinations, heart, lung, and spleen had no lesion, but liver, kidney, and brain showed various lesions. Degenerative lesions like intracytoplasmic eosinophilic inclusions were seen in both kidney and liver but fatty change was seen only in liver. There was no relationship between severity of the liver lesions and drug dosage. Central chromatolysis, scattered neuronal necrosis, and mild spongy changes were observed in five regions of the brain that were chosen for sectioning (motor cortex, cerebellar nuclei, midbrain nuclei, and hindbrain nuclei at two separate levels). Severity of lesions in brain was dose-dependent, and cerebral cortex was the most vulnerable area. Haematologic tests showed lower values for hematocrit and red blood cell count dose-dependently. In conclusion, artemisinin is a promising drug for prevention and control of coccidiosis in broiler chickens and its side effects are not too much serious especially at therapeutic doses.     

Seroprevalence of brucellosis and its contribution to abortion in cattle,camel, and goat kept under pastoral management in Borana,Ethiopia

The involvement of Brucella infection in causing abortion was investigated in a breeding female subpopulation of 283 cattle, 756 camels, and 757 goats. Serum samples were serially tested using the Rose Bengal test and complement fixation test. The study showed that anti-Brucella antibodies were prevalent in 10.6% (95% confidence interval (CI), 7.4, 14.9), 2.2% (95%CI, 1.4, 3.7), and 1.9% (95%CI, 1.1, 3.2) of cattle, camel, and goats, respectively. Abortion was more commonly reported in camels (23.4%) than cattle (13.8%) and goats (12.4%). The results of this study suggested that Brucella infections contribute significantly to abortion in cattle (odds ratio (OR), = 4.7; 95%CI, 2.0, 10.8) and goats (OR = 6.9; 95%CI, 2.2, 21.7) but not in camels. The number of young animals produced by breeding females seems to be apparently reduced in seropositive groups. Keeping more than two animal species at household level was found to be the risk factor for cattle (OR = 3.1; 95%CI, 1.2, 7.9) and camel (OR = 5.3; 95%CI, 1.2–23.5) seropositivity to Brucella infection when compared to those animals from households that keep only two animal species. This may suggest a possibility of cross species transmission of Brucella infection under such mixed herding. Wet season (OR = 4.8; 95%CI, 1.3, 18.1) was found to be associated with seropositivity in goats, linked to a coincidence of increased deliveries in flocks with possible excretion of Brucella organisms. The study results suggest that Brucella infection is the likely cause of abortion in cattle and goats while other causes largely outweigh brucellosis as a cause of abortion in camels in Borana, hence, contributing to reproductive loss.     

Effect of biotin supplementation on hoof health and ceramide composition in dairy cattle

The effect of biotin supplementation on various foot lesions and hoof ceramide composition of toe (wall) and sole portions of hooves was studied in crossbred dairy cattle. Biotin supplementation was done for five months in 14 cattle at a farm and the other 14 animals kept as control. A significant decline was observed in heel erosions and sole avulsions along with total disappearance of white line fissures and double soles in the biotin supplemented cattle resulting in decrease in the overall disease score. Thin layer chromatographs of the hoof lipids revealed 11 types of ceramides in sole lipids and 6 types of ceramides in toe (wall) lipids. The ceramides were typed and identified according to their Rf values. A qualitative increase in the density of thin layer chromatographs of sole lipids was observed in biotin supplemented cattle whereas a non-significant difference in density of thin layer chromatographs of toe lipids was observed after supplementation of biotin.     

Improvement in the diagnosis of <Emphasis Type="Italic">Brucella abortus</Emphasis> infections in naturally infected water buffaloes (<Emphasis Type="Italic">Bubalus bubalis</Emphasis>) using an ELISA with a Protein-G-based indicator system

Brucellosis caused by Brucella abortus in domestic water buffaloes (Bubalus bubalis) raised under the traditional system of husbandry in northern India was diagnosed using an enzyme-linked immunosorbent assays (ELISA) with a Protein-G-based indicator system (Protein-G ELISA). A total of 1,551 animals that are positive (N = 61), negative (N = 243), and suspected (N = 1,247) for brucellosis were examined. Rose bengal test (RBT) was used to predict the disease, and accordingly, animals were dichotomized in positive and negative population for receiver operating characteristic (ROC) curve analysis to determine the sensitivity, the specificity, and the performance index of Protein-G ELISA. Taking all animals (N=1551) into account, the ROC curve analysis revealed cut off value of 29.6% positivity (%P) with 98.40% and 94.94%, sensitivity and specificity, respectively. The results were compared with ELISA in which anti-bovine conjugate was used. The cut off in ELISA was 37.9%P and sensitivity and specificity were 96.26% and 97.07%, respectively. The performance indexes of both the assays were almost equal and were 193.34 for Protein-G ELISA and 193.33 for ELISA. The cut off values of both the tests changed, if only known positive (N = 61) and known negative (N=243) animals were used for ROC curve analysis, and accordingly, changes in sensitivity and specificity were observed with significant decrease of performance indexes of both the tests. The high optical density (P<0.0001) background signal with negative serum control and high %P (P<0.0001) in sera from negative population were noticed in ELISA in comparison to Protein-G ELISA.     

In vitro inhibitory effect of trichostatin A on canine grade 3 mast cell tumor

Mast cell tumor (MCT) is one of the most prevalent neoplasms that affect skin and soft tissue in dogs. Because mast cell tumors present a great variety of clinical appearance and behavior, their treatment becomes a challenge. Trichostatin A (TSA), an antifungal antibiotic, has shown inhibitory effects on the proliferation and induction of apoptosis in various types of cancer cells. In order to evaluate the potential of trichostatin A as a therapeutic drug, cells of grade 3 MCT were cultured and treated with concentrations of 1 nM to 400 nM of TSA. MTT assay and trypan blue exclusion assays were performed to estimate cell growth and cell viability, and cell cycle analysis was evaluated. TSA treatment showed a reduction in numbers of viable cells and an increase of cell death by apoptosis. The cell cycle analysis showed an increase of hypodiploid cells and a reduction of G0/G1 and G2/M –phases. According to these results, trichostatin A may be an interesting potential chemotherapeutic agent for the treatment of canine MCT.     

Characterization of Doayo and Kapsiki taurine cattle breeds of Cameroon in their natural environment

Data were collected on live weight (LW), heart girth (HG), height-at-withers (HW), trunk length (TL), age, sex, and coat color of 207 taurines cattle—122 of the Doayo (Namchi) breed and 85 of the Kapsiki (Kirdi) breed. The animals, aged 1 to 20 years, were selected from 60 herds randomly selected from villages of Poli of Faro and Mokolo of Tsanga, divisions of the North and Far North Regions of Cameroon. The data were analyzed using the SAS program with a linear model, applying standard tests. Results indicated no breed effect (P > 0.05) in the growth trends of LW, HG, HW, and TL. HG and TL were highly significantly (P < 0.0001) related to LW. The growth pattern for the two breeds was the same since the linear contrast of least square means for the traits at various age groups did not differ (P > 0.05) significantly. The breeds attained maturity as from 4 years. In the absence of breed effect (P > 0.05), a single regression equation was established for the estimation of live weight as thus LW = - 244.42 ( ±22.57 ) kg + 2.49 ( ±0.23 ) HG + 1.04 ( ±0.25 ) TL {\hbox{LW}} = - {244}.{42 }\left( {\pm {22}.{57}} \right){\hbox{ kg}} + {2}.{49 }\left( {\pm 0.{23}} \right){\hbox{ HG}} + {1}.0{4 }\left( {\pm 0.{25}} \right){\hbox{ TL}} , with HG contributing up to 70% of total variation and TL, 2%. This equation could be used to develop a measuring band useful in the rural environment for commercial and clinical veterinary purposes.