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51.
Christopher R. Thornton 《European journal of plant pathology / European Foundation for Plant Pathology》2008,121(3):347-353
Species of the genus Trichoderma are ubiquitous soil-borne fungi that exhibit antagonism towards a number of economically important plant-pathogenic fungi
and oomycetes. This review discusses recent developments in the use of monoclonal antibodies to detect these fungi in their
natural soil environments and to quantify their population dynamics during antagonistic interactions with saprotrophic competitors
in soil-based systems. Immunological approaches to detection and quantification are examined in relation to conventional plate
enrichment techniques and to nucleic acid-based procedures. An example of recent research using a mAb-based assay to quantify
the effects of saprotrophic competition on the growth of Trichoderma isolates in mixed species, soil-based, microcosms is presented. Future technological developments in immunoassays for tracking
Trichoderma populations in soil are discussed and results presented showing the accurate detection and visualization of a plant growth-promoting
isolate of T. hamatum in the rhizosphere of lettuce using mAb-based immunodiagnostic assays. 相似文献
52.
Feeding Solanum glaucophyllum to preparturient multiparous cows prevents postparturient hypocalcemia 下载免费PDF全文
Junichiro Ishii Akifumi Uramoto Yoshikazu Nagao Hisaya Goto 《Animal Science Journal》2015,86(10):869-877
Solanum glaucophyllum (SG) contains 1,25‐dihydroxyvitamin D3 (1,25‐(OH)2D3) glycosides. We investigated the effect of SG on hypocalcemia in cows. Serum levels of 1,25‐(OH)2D3, total calcium and phosphorus dose‐relatedly increased after feeding with SG, while serum magnesium and chloride levels fell (P < 0.05). We also performed an ethylenediaminetetraacetic acid (EDTA) infusion to induce artificial hypocalcemia. Cows that had been fed 4.0 mg/kg body weight of SG daily for 2 weeks had a higher serum concentration of total calcium at the end of EDTA infusion than those not fed SG (P < 0.05). In a field trial, multiparous cows were assigned to one of four groups: (1) no SG, (2) 1.3 g or (3) 2.6 g of SG daily from 14 days before the estimated calving day until 3 days after calving, or (4) a single feed of 35.75 g SG at 3 days before the estimated calving day. The concentrations of serum total calcium after the calving in each treatment group were (1) 7.4, (2) 7.9, (3) 8.0 and (4) 8.9 mg/dL and higher for (4) than for (1) (P < 0.05). The data suggests that feeding a high dose of SG before the calving may maintain higher concentrations of serum calcium after the calving. 相似文献
53.
Huimin Yang Murray Unkovich Ann McNeill Xianzhi Wang 《Biology and Fertility of Soils》2011,47(4):377-385
Symbiotic N2 fixation by lucerne (Medicago sativa) has capacity to provide significant inputs of N to agro-ecosystems, and the species has also been shown to scavenge soil
mineral N and thus act as a sink for excess reactive N. The balance between these two N cycle processes was investigated in
an extensive irrigated lucerne growing region where nitrate contamination of groundwater has been reported. We sampled 18
permanent pure lucerne stands under irrigation for standing dry matter, total shoot N, and N2 fixation using 15N natural abundance along with activity of the inducible enzyme nitrate reductase as indicators of use of soil NO3− by lucerne. On average 65% of lucerne N was obtained from symbiotic N2 fixation. Converting standing dry matter estimates to annual N2 fixation amounts we calculated average N2 fixation of 311 kg N/ha, including N in roots and nodules. Uptake of N from soil by lucerne was calculated to be 181 kg N/ha/year.
We were not able to identify the source of this soil mineral N, although nitrate reductase activity of lucerne was higher
than that of non-N2 fixing species examined. 相似文献
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Umma Tamima 《林业研究》2016,27(4):853-861
The objective was to evaluate the performance of the co-management of Nishorgo Support Project at Chunati Wildlife Sanctuary in Bangladesh. I adopted the Focus Groups Discussion method for opinion survey and applied the SWOTAHP technique for data analysis. Local people did not participate in the decision-making process of forest management and they perceived co-management as a threat to their livelihoods. Moreover, mistrust and misunderstanding among different stakeholders, political and ethical conflicts, lack of property rights of tribal communities, and lack of accountability and transparency were identified as the drawbacks of co-management. 相似文献
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