Harnessing the potential for otolith microchemistry to foster the conservation of Amazonian fishes

1. Freshwater environments host roughly half of the world’s fish diversity, much of which is concentrated in large, tropical river systems such as the Amazon. Fishes are critical to ecosystem functioning in the Amazon River basin but face increasing human threats. The basic biology of these species, and particularly migratory behaviour, remains poorly studied, in part owing to the difficulty associated with conducting tagging studies in remote tropical regions.
2. Otolith microchemistry can circumvent logistical issues and is an increasingly important tool for studying fish life histories. However, this approach is still new in the Amazon, and its potential and limitations to inform fish conservation strategies remain unclear.
3. Here, otolith microchemistry studies in the Amazon are reviewed, highlighting current possibilities, and several key factors that limit its use as a conservation tool in the Amazon are discussed. These include the dearth of spatiotemporal elemental data, poor understanding of environment–fish–otolith pathways, and insufficient funding, facilities, and equipment.
4. A research initiative is proposed to harness the potential of this technique to support conservation in the Amazon. Key aspects of the proposal include recommendations for internal and external funding, which are critical to acquiring and maintaining technical staff, cutting-edge equipment, and facilities, as well as fostering regular scientific meetings and working groups. Meetings can facilitate a systematic approach to investigating environment–otolith pathways, broadening the chemical baseline for most Amazonian tributaries, and exploring potential valuable elements.
5. These outcomes are urgently needed to conserve biodiversity and ecosystem functioning in the Amazon, especially given threats such as widespread hydroelectric damming. The initiative proposed here could make otolith microchemistry an important, cost-effective tool to inform and foster conservation in the Amazon, and act as a template for other imperilled tropical river basins, such as the Mekong and the Congo.

     

Comparison of bronchoalveolar lavage and respiratory secretion cytology in horses with clinically diagnosed chronic pulmonary disease

Thirty-nine horses and 3 ponies underwent a thorough respiratory examination and were grouped as follows: healthy (4 horses and 1 pony); mild chronic pulmonary disease (CPD 11 horses); moderate CPD (16 horses and 1 pony); and severe CPD (8 horses and 1 pony). Bronchoalveolar lavage (BAL) fluid collected from all animals and respiratory secretions (RS) obtained from 39 of these animals were evaluated cytologically and the results were compared. It was concluded that cytological examination of either BAL fluid or RS was useful in diagnosing various equine pulmonary diseases. The only advantage that BAL offered over RS sampling was in cases in which there was no RS available in the trachea. In addition, the severity of the CPD did not always correlate with either RS or BAL cytology.     

Preliminary experiences with the treatment of shock in horses with a plasma expander from a starch base

HAES Steril 10% is a colloidal plasma expander rarely used in veterinary medicine. In this study HAES was used in clinical cases for the treatment of shock and in a comparative hypervolemic hemodilution study (HAES versus lactated Ringer's solution) using two experimental horses. Injection of a HAES volume equivalent to 10% of estimated blood volume resulted in a highly significant drop in PCV and in a significant drop in total protein concentration. Half live of HAES was approximately two hours. No incompatibility reactions were observed. In man HAES improves microcirculation. Studies in progress may confirm a similar effect in the equine.     

Hydroxamic acid content of triticum species

Summary Fifty-five accessions of Triticum species were analyzed for content of hydroxamic acids (Hx), a natural resistance factor against various organisms. Hx were found in all accessions analyzed. Extreme values were found in wild diploid species: highest in T. speltoides (16.0 mmol/kg fr. wt) and lowest in T. tauschii (0.21). Modern polyploid wheats sharing the same genome did not show substantial variations in Hx levels. The data suggest possible sources of high Hx levels for wheat breeding programs.     

Callose concentration in leaves of field grown Oxalis acetosella (L.) indicates growth impediments

The Höglwald, located in Bavaria, is a pure Norway spruce stand, where experimental plots which were limed or not were subjected to normal or acid irrigation (Höglwald Experiment). The growth of the forest herb Oxalis acetosella improved greatly with liming. Callose concentration in leaves of Oxalis reflected the lime effect with about a 2.5 times lower callose concentration in the limed compared to unlimed plots. Acid irrigation increased callose concentration only in the unlimed plot. A negative exponential relationship was established between leaflet area and callose concentration. In a Diagnostic Field Fertilization Experiment in the vicinity of the Höglwald Experiment, Ca and Mg were applied either as carbonate or sulphate and Mn and Si added to experimental plots. In this experiment, it was demonstrated that it was not liming per se, but the application of Ca, that greatly increased the growth of Oxalis. Again, there was a negative correlation between leaflet area and callose concentration in leaves. Chemical analysis of leaves revealed that a Mn concentration > 3.5 mg (g DW)^?1 and a Mg concentration > 10 mg (g DW)^?1 led to increased callose concentration.     

Ertragsentwicklung im Dauerversuch Ewiger Roggenbau Halle nach den 1990 vorgenommenen Umstellungen in der Düngung

Yield development in the long-term experiment Continuous Rye Cropping Halle after the changes in the fertilization in 1990 The long-term experiment Continuous Rye Cropping being established 1878 on a degraded chernozem (from sandy loess) includes among others a treatment with application of mineral N only over 112 years in which during the last decades the grain yields ranged about 30% below that on the plots with farmyard manure (FYM) or complete mineral fertilization (NPK). The considerable depletion of available P and K in the respective soil was practically overcome in 1990 by a single application of 200 kg P ha^?1 and 400 kg K ha^?1 and thereafter the exclusive fertilization with mineral N was substituted by a combined application of NPK and FYM. Already in the first year the previous yield decline in comparison to ‘NPK’ or ‘FYM’ had been overcome completely. An additional yield increase, however, could only be realized under conditions especially favourable for yield production, so in 1993 and 1995.     

EMS and UV irradiation induce unstable resistance against CAA fungicides in <Emphasis Type="Italic">Bremia lactucae</Emphasis>

Wild type (WT) field isolates of Bremia lactucae failed to germinate in vitro or infect lettuce leaves in the presence of CAA (carboxylic acid amide) fungicides. Minimal inhibitory concentrations (MIC) for mandipropamid, dimethomorph, benthiavalicarb and iprovalicarb were 0.005, 0.5, 0.5 and 5 μg ml⁻¹, respectively. Mutagenesis experiments showed that spores exposed to EMS (ethyl methane sulphonate) or UV irradiation (254 nm) could infect lettuce leaves in the presence of up to 100 μg ml⁻¹ CAA. The proportion of infected leaves relative to the number of spores inoculated (infection frequency) was inversely related to the concentration of CAA used, ranging between 0 and 160 per 1 × 10⁶ spores. Resistant mutants (RM) lost their resistance within 1–14 reproduction cycles on CAA-treated plants. Crosses were made between RMxWT isolates and RMxRM isolates with an attempt to obtain stable homozygous resistant off-springs. Such crosses yielded few resistant but unstable progeny isolates. Mutagenic treatments given to hybrid isolates also failed to produce stable resistance. Previous gene sequencing data showed that stable resistance to CAAs is based on a single SNP in the cellulose synthase 3 (CesA3) gene of Plasmopara viticola. Therefore, we sequenced a 582 bp DNA fragment of Ces3A of WT, RM and hybrid isolates of B.lactucae. No mutation in this gene fragment was found. We conclude that mutagenic agents like EMS or UV may induce resistance to CAA in Bremia lactucae but this resistance is not stable and not linked to mutations in CesA3 gene.     

Organic-geochemical Differentiation of Petroleum-type Pollutants and Study of Their Fate in Danube Alluvial Sediments and Corresponding Water (Pančevo Oil Refinery, Serbia)

A review is given in this paper of the up-to-date results observed in differentiation and transformation studies on petroleum-type pollutants in underground and surface waters. Water and particulate matter derived from the locality of Pan?evo Petroleum Refinery, Serbia (River Danube alluvial formations). It was shown that distributions of n-alkanes, steranes and triterpanes, and δ¹³C_PDB values of n-alkanes may successfully be used for qualitatively differentiating the petroleum-type pollutants from native organic matter in recent sedimentary formations. In underground waters, a petroleum-type pollutant is exposed to microbiological degradation which is manifested through relatively fast degradation of n-alkanes. Following an almost complete degradation of crude oil n-alkanes in underground water, the biosynthesis of novel, even carbon-number C₁₆–C₃₀ n-alkanes may be observed. It is shown that the n-alkane distribution observed in a petroleum-type pollutant may depend on the intensity of its previous interaction with water. The fate of petroleum-type pollutants in environmental waters may be predicted through laboratory simulative microbiological degradation experiments by using microorganism consortiums similar to those observed under relevant natural conditions, as well as on corresponding nutrient base.     

Genetic diversity in sweetpotato [Ipomoea batatas (L.) Lam.] in relationship to geographic sources as assessed with RAPD markers

Available evidence shows that sweetpotato originated from either Central or South American lowlands with subsequent dispersal to North America, Europe, Africa, Asia and the pacific islands. A total of 71 polymorphic RAPD molecular markers were used to assess the genetic relationships amongst 74 sweetpotato varieties originating from a total of 23 sweetpotato producing countries within six geographical regions, namely, South America, Central America/Caribbean, United States of America (USA), East Africa, Asia and Oceania. An Analysis of Molecular Variance (AMOVA) indicated that 93.4% of the total variance was due to the differences between genotypes within regions. The difference between regions was significant (P < 0.001) but only contributed 6.6% to the variance. Genetic distance (PhiST) calculated with AMOVA and multidimensional scaling (MDS) revealed that the South American and the Central American/Caribbean genotypes formed two separate clusters. East African varieties, which have unique characteristics from other traditional varieties, were distinct from other traditional varieties from South America and Oceania. These results support the reported hypothesis of the origin and dispersal of the sweetpotato and indicate that the primary centre of diversity probably has two distinct genepools. It is proposed that the dispersal of the sweetpotato from its origin may have mainly involved varieties from Central America/Caribbean as opposed to varieties from South America. There is an indication that new genepools may be evolving in Africa and Asia due to hybridisation and adaptation to the local environments.     

Beziehungen zwischen Kohlenstoff-und Stickstoffmetabolismus bei Pflanzen — eine Übersicht über ausgewählte Aspekte

Zusammenfassung Die mannigfaltigen Wechselbeziehungen zwischen photosynthetischer Kohlenstoffassimilation und N-Metabolismus haben weltweit eine Vielzahl von Untersuchungen stimuliert. Es ist versucht worden, zu einigen ausgewählten Aspekten dieses komplexen Problemkreises eine Übersicht über den gegenwärtigen Kenntnisstand zu geben. Von den Prozessen auf molekularer Ebene wurden besonders der photorespiratorische N-Zyklus und die Beziehungen zwischen Photosynthese und Nitratassimilation berücksichtigt. Auf der organismischen Ebene spielen Wechselwirkungen zwischen Photosynthese und N-Haushalt in bezug auf die Ertragsbildung eine besondere Rolle. Sie wurden am Beispiel des Getreides erörtert. Mathematische Modelle der Photosynthese und Ertragsbildung haben in stärkerem Maße als bisher dem Einfluß des N-Metabolismus Rechnung zu tragen. Daher wurde der Darstellung dieses Problemkreises besondere Aufmerksamkeit gewidmet.

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Interactions between carbon and nitrogen metabolism in plants — a review of some selected topics

Summary The multiple interactions between photosynthetic carbon assimilation and nitrogen metabolism have stimulated worldwide investigations. In the present review some selected topics of this field are discussed. At the molecular level the photo-respiratory nitrogen cycle and relationships between photosynthesis and nitrate assimilation are especially considered. At the whole plant level relationships between photosynthesis and N metabolism are of importance with regard to yield formation of cultivated plants. As an example the situation in cereals is discussed. Mathematical models of photosynthesis and yield formation have to consider the influence of nitrogen metabolism on these processes. Therefore this aspect was treated in some detail.

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