Nitrogen availability decreases prokaryotic diversity in sandy soils

In this study, the interrelation between nitrogen availability and prokaryotic diversity are studied using a well-characterised system from a long-term field experiment on a loamy sandy soil. The prokaryotic potential functional diversity and community composition were assessed using community-level physiological profiling (CLPP), and their phylogenetic diversity was analysed using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) in combination with sequencing analysis. Highest prokaryotic potential functional diversity was measured in the control soil receiving no N fertilisation, indicating an efficient as well as versatile utilisation of the substrates in this soil. Both substrate utilisation richness and substrate utilisation evenness, the two constituents of the functional diversity, were decreased with increasing N supply. Furthermore, distinct prokaryotic community compositions were generated in N-enriched soils compared to unfertilised control soils. These differences suggest a dominance of populations adapted to utilising readily available substrates. We demonstrated that the shift in prokaryotic functional diversity was connected to a shift in the phylogenetic structure of the bacterial and archaeal communities. Taken together, our data clearly show that, for the sandy soil system, prokaryotic diversity and N availability were interrelated.     

Passive and Active Sampling of Benzene in Different Urban Environments in Gothenburg, Sweden

Urban benzene concentration and personal exposure to benzene were measured within different urban environments in Gothenburg, Sweden. Three intensive monitoring campaigns were carried out in May 2000, February 2001 and May 2001. Three different environments were selected; two central built-up areas with different traffic densities (high and low) and one large urban park. The methods used included passive and active sampling. Passive sampling was used to retrieve spatial variability in urban benzene concentrations, while active sampling aimed to give data on personal exposure levels within the designated areas. The passive sampling showed large spatial and temporal variations in urban benzene concentrations. The benzene concentrations, ranging from 1.0 to 3.1 μg m^?3, were typically two to four times higher within the high-traffic built-up area compared to the adjacent urban park. This was mainly due to the proximity to motorised traffic. A comparison between personal exposure and active stationary measurements showed that the personal exposure levels were roughly 1.5 times higher in the heavy traffic area and 1.3 times higher in the light traffic area than the fixed benzene concentrations. The personal exposure levels in the park were equal to the active stationary benzene concentration. Given the large spatial heterogeneity within urban areas, the placement of single street level point measurements becomes crucial. Care must be taken to ensure that the measurements are actually representative when using these measurements to estimate urban concentrations and exposure levels.     

Monitoring fish stocks in relation to acidification in Norwegian watersheds

The Norwegian Monitoring Programme for Long-Range Transported Air Pollutants started in 1980. The biological part of this programme includes besides invertebrate studies in streams, (i) fish community status in lakes by means of interviews, test-fishing in lakes by using standard gill-net series, recruitment studies of brown trout in inland streams, and juvenile stock assess and monitoring of fish kills in salmon rivers. Damaged fish stocks are recognized within a land area of 51,500 km² in southern Norway and 30 km² in northern Norway. At least 6,000 lake-dwelling fish stocks have either been lost or are at various stages of reduction. Brown trout (Salmo trutta) is the most widespread and abundant species of fish in Norwegian watersheds, and is also most severe affected by acidification. More recently, there are some indications of an increase in the abundance of brown trout in some areas. However, analysis of age structure in lakes, and fry densities in streams in such areas revealed large annual variations in recruitment rate, which indicates unstable water chemical conditions. Atlantic salmon (Salmo salar) is virtually extinct in 25 rivers in southernmost, southwestern and western Norway.     

Significance of vitamin E supplementation, dietary content of polyunsaturated fatty acids, and preslaughter stress on oxidative status in pig as reflected in cell integrity and antioxidative enzyme activities in porcine muscle

The present study investigates the combined effects of feed-induced increase in polyunsaturated fatty acids (PUFA) content and/or alpha-tocopherol content in pig muscles and preslaughter stress on cell integrity. Cell integrity was determined by plasma lactate dehydrogenase (LDH) activity, and antioxidative status of muscle was measured by activities of the antioxidative enzymes catalase, superoxide dismutase, and glutathione peroxidase. Preslaughter stress increased LDH activity, reflecting loss in cell membrane integrity independent of increased content of PUFA and/or alpha-tocopherol. However, feed-induced increase of PUFA decreased the LDH activity in plasma immediately after slaughter. Catalase activity in the muscle tissue increased as a consequence of the high-PUFA diet, which may indicate an increased demand caused by introduction of oxidative labile PUFA.     

A general model for assessment of the carbon sequestration potential of afforestation projects

Among the objectives of afforestation projects, carbon sequestration is attracting increasing political attention. Therefore, there is a need for understanding the carbon sequestration ??mechanism?? and for ex-ante assessment of the sequestration potential of afforestation projects. Such assessment must be based on parameter estimates that are inherently uncertain, making the basis for applying an advanced sequestration model weak. This paper presents a transparent, simple and general model for quantification of the carbon sequestration in afforestation projects. The model can easily be modified so as to fit specific conditions, and it is held sufficiently reliable when taking into account the ex-ante character of the decision problem. The use of the model is exemplified by application to a yield table for Sitka spruce. Limitations of the model framework and its application in combination with scenarios of climate change are discussed.     

Effects of grassland abandonment, restoration and management on butterflies and vascular plants

When semi-natural pastures are abandoned, specialized grassland species are lost as a consequence of succession. As a counter-measure, previously abandoned grasslands may be restored by clearing shrubs and trees and re-introducing grazing livestock. In order to examine the effects of this type of habitat restoration, we compared species richness of plants and of specialized plants thought to be dependent on continuous management and species richness and abundance of butterflies and red-listed butterflies in 12 sets of matched continuously managed, abandoned and restored grassland in southern Sweden. We found no differences in species richness or abundance between the three grassland types. There were, however, some negative effects of abandonment. The number of management-dependent plants decreased with increasing cover of trees and shrubs, and in restored sites species richness of all groups decreased with increasing cover of trees and shrubs before restoration. Also the present management significantly affected both butterflies and plants. Species richness of both groups increased with increasing vegetation height and differed between sites depending on the species of grazers, with negative effects of sheep compared to cattle or horses. Our study indicates that for grassland management to be efficient, the restoration actions should mainly be directed towards sites where the post-abandonment succession has not proceeded too far.     

Total folate content and retention in rosehips (Rosa ssp.) after drying

Folate concentrations in rosehips and commercial rosehip products and factors affecting folate retention during drying were investigated. On the basis of the raw material studied during 3 years, rosehips were shown to be a rich folate source, 400-600 microg/100 g based on dry matter and 160-185 microg/100 g based on the fresh weight (edible part). Rosehips are not often consumed fresh; therefore, drying to produce stable semimanufactures is a crucial step. The degradation of folate was shown to be dependent on the drying time until the water activity was below 0.75. The required drying time was reduced by cutting the rosehips in slices and to some extent also by increasing the temperature. Retention of folate and ascorbic acid was affected by the same factors, and high content of ascorbic acid could provide a possible protection for folate degradation.     

Inorganic Constituents of Well Water in One Acid and One Alkaline Area of South Sweden

Acid precipitation may lead to loss of essential elements and increase the concentrations of potentially toxic elements in drinking water. In this study 46 private wells from acid regions(pH < 6.5) were compared with 43 private wells from alkaline areas in southern Sweden. The concentrations of about 30 elementswere analysed especially by inductively coupled plasma opticalemission spectroscopy (ICP-OES). The concentrations of essentialelements such as calcium, chromium, selenium and potassium weresignificantly lower in acid than in alkaline well water. On theother hand, the levels of potentially toxic metals such as cadmium and lead were significantly higher in acid well water. High copper concentrations, observed at pH around 6 in contrast to earlier findings, is to be considered as an acidification problem, as should the high fluoride values. The highest concentrations of a number of metals and ions, for example calcium, chromium, titanium and sulphate, appeared at pH 7.0–8.0, where the peak in concentrations occur due to leachingof metals from soil particles in acid soils and precipitation ofcarbonates and sulphates in more alkaline soils. The low levelsof especially calcium and magnesium ions, and some micronutrientsin the acid water, in combination with high concentrations of acid ions and toxic microelements, may cause nutritional imbalances. This should be regarded as risk factors with relationto effects on human health.     

ICP Waters — an International Programme for Surface Water Monitoring

The International Cooperative Programme on Assessment and Monitoring of Acidification of Rivers and Lakes (ICP Waters) was established in July 1985 under the United Nations Economic Commission for Europe (UN/ECE) Executive Body of the Convention on Long-Range Transboundary Air Pollution (LRTAP). The Programme Centre is at the Norwegian Institute for Water Research, Oslo. The main aim of the Programme is to assess, on a regional basis, the degree and geographical extent of acidification of surface waters. 23 countries in Europe and North America participate on a regular basis. The major findings in the programme cover (1) regional trends in water chemistry related to changes in S- and N-deposition, (2) dose/response relationships particularly between biology (invertebrates) and water chemistry (acidification status), (3) effects of acidification on aquatic fauna, (4) implications for the assessment of critical loads, and (5) nitrogen leaching. Both chemical and biological intercalibrations and intercomparisons are important parts of the programme activities.