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21.
In the wake of a scrapie monitoring programme 2002 in Mecklenburg-Westpomerania a total of 10 positive cases were identified after culling at a suffolk-farm with about 1000 animals. Scientific based recommendations are difficult to judge as longitudinal studies and subsequent quantitative risk factors regarding the epidemiology of scrapie are either not available or conflicting. Given this background a risk assessment was developed that is based on the probability of validated risk factors considering special husbandry and circumstances at the very farm. 相似文献
22.
Kayser H Lee C Decock A Baur M Haettenschwiler J Maienfisch P 《Pest management science》2004,60(10):945-958
Neonicotinoids bind selectively to insect nicotinic acetylcholine receptors with nanomolar affinity to act as potent insecticides. While the members of the neonicotinoid class have many structural features in common, it is not known whether they also share the same mode of binding to the target receptor. Previous competition studies with [3H]imidacloprid, the first commercialised neonicotinoid, indicated that thiamethoxam, representing a novel structural sub-class, may bind in a different way from that of other neonicotinoids. In the present work we analysed the mode of [3H]imidacloprid displacement by established neonicotinoids and newly synthesized analogues in the aphids Myzus persicae Sulzer and Aphis craccivora Koch. We found two classes of neonicotinoids with distinct modes of interference with [3H]imidacloprid, described as direct competitive inhibition and non-competitive inhibition, respectively. Competitive neonicotinoids were acetamiprid, nitenpyram, thiacloprid, clothianidin and nithiazine, whereas thiamethoxam and the N-methyl analogues of imidacloprid and clothianidin showed non-competitive inhibition. The chloropyridine or chlorothiazole heterocycles, the polar pharmacophore parts, such as nitroimino, cyanoimino and nitromethylene, and the cyclic or acyclic structure of the pharmacophore were not relevant for the mode of inhibition. Consensus structural features of the neonicotinoids were defined for the two mechanisms of interaction with [3H]imidacloprid binding. Furthermore, two sub-classes of non-competitive inhibitors can be discriminated on the basis of their Hill coefficients for imidacloprid displacement. We conclude from the present data that the direct competitors share the binding site with imidacloprid, whereas non-competitive compounds, like thiamethoxam, bind to a different site or in a different mode. 相似文献
23.
Neonicotinoids represent a class of insect-selective ligands of nicotinic acetylcholine receptors. Imidacloprid, the first commercially used neonicotinoid insecticide, has been studied on neuronal preparations from many insects to date. Here we report first intrinsic binding data of thiamethoxam, using membranes from Myzus persicae Sulzer and Aphis craccivora Koch. In both aphids, specific binding of [3H]thiamethoxam was sensitive to temperature, while the absolute level of non-specific binding was not affected. In M persicae, binding capacity (Bmax) for [3H]thiamethoxam was ca 450 fmol mg(-1) of protein at 22 degrees C and ca 700 fmol mg(-1) of protein at 2 degrees C. The negative effect of increased temperature was reversible and hence not due to some destructive process. The affinity for [3H]thiamethoxam was less affected by temperature: Kd was ca 11 nM at 2 degrees C and ca 15 nM at 22 degrees C. The membranes also lost binding sites for [3H]thiamethoxam during prolonged storage at room temperature, and upon freezing and thawing. In A craccivora, [3H]thiamethoxam was bound with a capacity of ca 1000 fmol mg(-1) protein and an affinity of ca 90 nM, as measured at 2 degrees C. Overall, the in vitro temperature sensitivity of [3H]thiamethoxam binding was in obvious contrast to the behaviour of [3H]imidacloprid studied in parallel. Moreover, the binding of [3H]thiamethoxam was inhibited by imidacloprid in a non-competitive mode, as shown with M persicae. In our view, these differences demonstrate that thiamethoxam and imidacloprid, which represent different structural sub-classes of neonicotinoids, do not share the same binding site or mode. This holds also for other neonicotinoids, as we report in a companion article. 相似文献
24.
25.
Pymetrozine, a pyridine azomethine compound, represents a novel insecticide with a selective activity against homopteran insects. It acts in a unique way: aphids are not knocked down on contact but seem to die of starvation. This implies an effect of pymetrozine on feeding behaviour. The aim of the present work was to elucidate how and at which step pymetrozine interferes with the complex mechanisms underlying phloem feeding. The effect of pymetrozine, applied in various ways, on different phases of stylet penetration and feeding activity of individual aphids was studied using the Electrical Penetration Graph technique (EPG). Initial choice experiments indicated that pymetrozine does not have a deterrent or antifeedant action. Topical application (150 ng pymetrozine mg-1 fresh weight) inhibited stylet insertion into the plant. When injected, less than 30 ng mg-1 was sufficient to produce the same effect. When pymetrozine was systemically applied via plant spraying or root uptake, aphids started feeding normally. After some time, however, they withdrew their stylets from the phloem and walked around with unaffected locomotion. At low doses aphids eventually recovered and resumed feeding. High doses, however, irreversibly disrupted feeding and prevented stylet reinsertion. Aphid motility was not affected up to an estimated haemolymph concentration of 1 mM pymetrozine. Aphids which eventually stopped feeding on pymetrozine-treated plants showed EPGs with distorted salivation/ingestion patterns. It is concluded that pymetrozine does not have a general toxic effect on aphids but selectively interferes with the nervous regulation of feeding behaviour which consequently results in death due to starvation after a few days. © 1997 SCI. 相似文献
26.
Carrageenan-induced acute inflammatory response in chicks 总被引:1,自引:0,他引:1
Carrageenan from two commercial sources was used in varying concentrations to evoke an acute inflammatory response in the foot pad of chickens. Oedema formation and increased vascular permeability were less severe using this experimental model than with turpentine-induced injury. Inflammation was monophasic, of short duration and apparently mediated by histamine and serotonin as their respective antagonists diminished the response. Prostaglandins seemed to play only a minor role as indomethacin blocked only the later phase of oedema. Bothrops jararaca venom did not potentiate the inflammatory response, implying that bradykinin was not a significant mediator. 相似文献
27.
Roos CF Riebe M Häffner H Hänsel W Benhelm J Lancaster GP Becher C Schmidt-Kaler F Blatt R 《Science (New York, N.Y.)》2004,304(5676):1478-1480
We report the deterministic creation of maximally entangled three-qubit states-specifically the Greenberger-Horne-Zeilinger (GHZ) state and the W state-with a trapped-ion quantum computer. We read out one of the qubits selectively and show how GHZ and W states are affected by this local measurement. Additionally, we demonstrate conditional operations controlled by the results from reading out one qubit. Tripartite entanglement is deterministically transformed into bipartite entanglement by local operations only. These operations are the measurement of one qubit of a GHZ state in a rotated basis and, conditioned on this measurement result, the application of single-qubit rotations. 相似文献
28.
Haack Udo K. Gutsche Frank H. Plessow Kirsten Heinrichs Hartmut 《Water, air, and soil pollution》2002,139(1-4):261-288
Fifty seven (57) cloud water samples collected in 1 hr intervals during 9cloud events in 1997 at the summit of the Brocken (altitude 1142 m) in Central Germany were analyzed for their Pb concentrations and isotopic compositions. For comparison, filterdusts of such possible emitters as power plants, cement factories, waste incinerators, cars and others were also studied.Profiles over many hours reveal changes in composition correlatedwith changes in the origin of the air masses. Five main groups ofpossible sources of the Pb can be distinguished: most importantare (1) the European standard pollution ESP dominating the continent from Germany over France to Scandinavia and (2) industrial Pb (and soot from car exhausts) followed by (3) urban waste incinerators as well as (4) perhaps ores from Norilsk/Siberia, and, somewhat uncertain, (5) ores from PaleozoicEuropean deposits. Local and daily varying anthropogenic emissions of the sources (2–5) cause scatter of the points around the ESP correlation line. In soils all these variationsand individual signatures are levelled out so that specific sources of pollution cannot be identified anymore. Geogenic Pbfrom rocks and soils is the same as that from power plants and cement factories. It is one of the two nominal end components making up the mixture of the ESP. The less radiogenic end component of this mix which must contain Pb from very old deposits is somewhat elusive: none of the large Archaic or Proterozoic deposits could be identified. Most probably numerous sources contribute to this Pb. Pb from leaded gasoline seems to be absent or is hidden under the five components dominating the composition of the Pb in the European environment. This probably reflects phasing out of such gasoline in most European countries. The Pb in the clouds may contain a small component of old Proterozoic or even Archaic Pb, but it can also be modelled with Paleozoic Pb. 相似文献
29.
Udo K. Haack Hartmut Heinrichs Frank H. Gutsche Kirsten Plessow 《Water, air, and soil pollution》2003,150(1-4):113-134
Seventy-five samples of six soil profiles from forests on different substrates plus one next to a motorway were analyzed for the variation of Pb concentration and isotopic composition with depth. A substantial fraction of the anthropogenic Pb (peak concentrations between 74 and 300 ppm) is still stored in the organic top soil, and seldom penetrates deeper than 20–30 cm. In (208Pb/206Pb) vs. (207Pb/206Pb)-diagrams the Pb in the uppermost layers of the profiles plots on an excellent correlation line (R = 0.99) indicating essentially a two-component mixing system. Values for the dust on Scottish plants, Norwegian and Swiss bogs as well as for aerosols collected over the North Atlantic (data from the literature) plot on the same correlation line showing that the Pb was deposited from a continent-wide mixing system with two apparent end members: The radiogenic one is similar to geogenic Pb and could consist of contributions from certain European ore deposits and from different soil components (e.g. silicates and Fe-oxides), power plants and cement factories. The less radiogenic component must contain or consist of Pb from Proterozoic or even Archaic ore deposits. However, the sources of this Pb remain somewhat elusive. In any case, this isotopic pattern is so consistent all over Europe that the correlation line may serve as a line of reference for quasi normal or European Standard Lead Pollution (ESLP). The gasoline derived Pb in the soils is swamped by Pb of other provenances. 相似文献
30.
The tilapias are one of the most important fish groups in aquaculture.In spite of this, relatively few workers have attempted to establish the feedration that would suffice for their maintenance. The results that they present,moreover, do not match, which may be attributed as much to differences inanalytical procedure as to experimental protocol. In this work, previous dataonred tilapia by the late Dr was therefore reassessed in order to arrive at more reliableresults. The dataset was based on fish between 15 and 20g wet body mass givenvarious levels (0, 0.5, 1 & 3% of body mass) of three protein level diets(low: 13.1%, LP; medium, 28.7%, MP; high: 43.9%, HP) at two temperatures (20.9& 24.3 °C). The required feeding level to maintain drybodyweight was 2.52, 2.81 and 7.05 g kg–0.8 fishweight per day for the HP, MP and LP diets respectively at the highertemperature and 1.92, 2.32 and 5.04 g kg–0.8day–1 at the lower temperature. This gave rise to anaverage Q10 coefficient of 2.22. The required feed quantity tomaintain body protein or body energy, however, was in most cases higher, partlybecause the fish initially had high protein and lipid and low ash content.Comparisons with other maintenance estimates for tilapia were also made and thereasons for discrepancies between the results obtained here and elsewherediscussed. 相似文献