秦川牛近交与近交衰退的分析

本文分析了陕西省秦川牛场秦川牛群近交及其对初生重、断奶重及哺乳期成活率的影响,并采用标准群体遗传学模型分析了秦川牛的致死当量。研究发现,30多年来秦川牛群平均近交系数呈明显的上升趋势,而且近交对成活率、初生重及断奶重均有一定危害;秦川牛群体的隐性有害基因含量较少,平均每个个体约携带2.05个致死当量。     

Monoclonal antibodies to bovine viral diarrhea virus: cross-reactivities to field isolates and hog cholera virus strains.

Monoclonal antibodies to bovine viral diarrhea virus (BVDV) were examined for binding with a large number of North American BVDV isolates and eight strains of the serologically related pestivirus, hog cholera virus (HCV). No single BVDV monoclonal antibody reacted with all BVDV isolates. The most cross-reactive monoclonal antibody was an anti-p80/p125 antibody which showed a positive reaction with 173 of 180 (96%) North American isolates. From a fewer number of isolates tested, one anti-gp53 monoclonal antibody also showed a high cross-reactivity (94%). All BVDV isolates showed a positive reaction with at least one of the seven monoclonal antibodies in the panel. Thus, the results indicated that a pool of these monoclonal antibodies may be used in place of polyclonal antisera for the detection of BVDV contamination of cell lines or for virus isolation. For HCV, all three anti-p80/p125 monoclonal antibodies reacted positively with all eight virus strains. In contrast, none of the anti-gp53 monoclonal antibodies were reactive to HCV strains. Thus, the anti-gp53 monoclonal antibodies may be useful for distinguishing between usually innocuous BVDV infections and the highly significant HCV infections in swine for foreign animal disease surveillance.     

Comparative effects of mu and kappa opiate agonists on the cecocolic motility in the pony.

The electrical and mechanical activity of the large intestine and its response to administration of opiate mu and kappa agonists were assessed from electrodes and inductograph coils chronically implanted on the cecocolic segment in six ponies given a diet of hay and concentrates. Before the drugs were given, migrating complexes propagating from the cecum into the colon occurred at the rate of 1.5 to 16/hour. During this propulsive activity, the cecocolic sphincter opened and closed allowing the outflow of cecal contents and preventing the backflow of colic contents. Each pony was used as its own control and was given fentanyl (0.01 and 0.05 mg/kg of body weight, IV) and U50488H (0.1 and 0.5 mg/kg, IV) at weekly intervals. The mu agonist fentanyl elicited a marked phase of inhibition of the propulsive activity and a closure of the cecocolic sphincter that lasted one to two hours depending on the dose. The kappa agonist U50488H induced an inhibition of the short spiking activity, i.e. of the resting muscle tone. It did not disturb the occurrence of migrating complexes nor that of the openings of the cecocolic sphincter. These kappa compounds may be drugs of choice to alleviate visceral pain in colic stases without inducing delay of transit unlike mu compounds.     

高维Mbius变换的类型判别法

本文利用高维Mbius变换的直角坐标表示式,找到了它们Poincaré扩张的具体式子,并研究了任意有限维不同类型的Mǒbius变换的系数的一些性质及不动点的位置,在此基础上建立了高维Mbius变换只依赖于系数的判别法.     

西农萨能奶山羊血浆生长激素水平的变化及其与奶产量的关系

本实验用放免测定法(RIA)测定了38只西农萨能奶山羊(其中360～450日龄的青年羊10只,产前一月至产后90天的头胎和2胎羊各14只)血浆GH水平变化,分析了血浆GH水平与奶量的相关性。结果表明:360～450日龄的青年羊血浆GH维持在一个波动较小的范围(20.37±5.17～23.13±5.97ng/ml),头胎羊和二胎羊分娩前后及泌乳早期血浆GH水平的变化趋势相一致,在同一生理状态下,二胎羊血浆GH水平都显著地高于头胎羊。相关分析还表明:头胎羊和二胎羊血浆GH水平都与其奶量呈直线正相关关系(P<0.05)。综上结果可以认为,奶山羊血浆GN水平可作为选育高产奶山羊的一项指标。     

森林资源管护经营责任制的实践与体会

通过八面通林区实施森林资源管护经营责任制的实践 ,论述森林资源管护经营责任制的实施、延续、发展及推行责任区经济的意义 ,阐明发展责任区经济同实施森林资源管护经营责任制之间的关系 ,为改革的发展和完善起到抛砖引玉的作用。     

双孢蘑菇覆土层有益微生物消长规律

将土样制备成稀释液，采用平板涂布培养法，分析双孢蘑菇覆土期、充分发菌期，原基形成期、采收前和采收后2d5个时期土样中几种有益微生物和消长规律。结果表明，原基表成前和采收后，采白色和采收后2d5下时期土样中几种有益微生物的消化规律。结果表明，原其开前和采收后，采白色孢杆菌(Bacillus)、固氮苗（Azotobacter)和假单胞杆菌（Pseudomonas)数量增加；链霉菌（Streptomyces)数量增减比较平缓，而土灰色芽孢杆菌的数量一直呈下降趋势。     

果树水分胁迫研究进展

从水分胁迫对果树叶、根的形态指标及显微结构 ,叶片气孔行为、光合作用、光抑制、活性氧代谢、脂氧合酶代谢、多胺代谢、脯氨酸、核酸代谢、内源激素变化等生理生化方面综述了近十几年来的研究成果 ,为全面研究果树抗旱机理及进一步制定抗旱措施奠定理论基础     

茉莉酸对苹果气孔运动及抗旱性的影响

50、75、100、150 mg／L JA处理苹果叶片表皮，使开放气孔的气孔开度分别减小27．4％、37．5％、56．9％和63．8％，使关闭后的气孔在光下开放时气孔开度分别减小15％、18％、28．8％、52．5％、61．3％；处理植株后干旱胁迫，叶片相对电导率分别降低15％、18％、31％、58％，脯氨酸含量分别提高0．09％、0．40％、0．42％、0．54 ng／gFW，可溶性糖含量分别提高1．89％、2．53％、3．80％、5．06％。25 mg／L JA处理与对照均无显著差别。JA的作用表现出明显的浓度效应。     

RAPD标记鉴定果树芽变的可行性分析

在综合分析果树芽变的遗传特点以及RAPD标记技术的原理与存在问题的基础上，结合已有的研究报道，对RAPD标记应用于果树芽变鉴定中的可行性进行了探讨。对于不同的果树芽变类型，RAPD标记技术表现出不同的鉴定可行性。同时，对某些有关的问题提出了相应的建议。