The gas phase oxidation of elemental mercury by ozone

The gas phase reaction between elemental mercury (Hg⁰) and ozone (0₃) has been studied in sunlight, in darkness, at different temperatures, and different surface-to-volume (s/v) ratios. At 0₃ concentrations above 20 ppm, a loss of Hg⁰ and a simultaneous formation of oxidized mercury (Hg(II)) was observed. The results suggest a partly heterogeneous reaction, with a gas phase rate constant of 3±2×10^?20 cm³ molec.^?1 s^?1 at 20 °C. This corresponds to an atmospheric Hg half-life of about one year at a mean global 0₃ concentration of 30 ppb.     

Immunoaffinity purification of chlorimuron-ethyl from soil extracts prior to quantitation by enzyme-linked immunosorbent assay

A competitive-indirect enzyme-linked immunosorbent assay (CI-ELISA) was developed to quantify chlorimuron-ethyl in soil. The linear working range of the assay was from 1 to 1000 ng mL(-)(1). The assay had an I(50) value of 54 ng mL(-)(1), with a limit of detection of 2 ng mL(-)(1) and a limit of quantification of 27 ng mL(-)(1). Three soils were extracted using a carbonate buffer (pH 9.0) and the extracts spiked with chlorimuron-ethyl. Because of the effects of coextractants (matrix effects) from soil on the accuracy and precision of the ELISA, immunoaffinity chromatography (IAC) was used to purify chlorimuron-ethyl from soil extracts prior to analysis. The immunoaffinity columns, which had a total binding capacity of 1350 ng of chlorimuron-ethyl mL(-)(1) of immunosorbent, were prepared by binding anti-chlorimuron-ethyl antibodies to protein G Sepharose 4B. Although the matrix effects were largely removed using the affinity column, they could be completely removed by first passing the extract through a column containing epoxy-coupled 1,6-diaminohexane (EAH) Sepharose 4B to remove organic acids prior to IAC. Assay sensitivity was increased 100-fold using IAC to purify and simultaneously concentrate chlorimuron-ethyl from soil extracts. The purification strategy (EAH followed by IAC chromatography) removed matrix effects from all three soils and allowed for the accurate quantitation of chlorimuron-ethyl in soil extracts.     

Amelioration of a hardsetting Alfisol through deep mouldboard ploughing, gypsum application and double cropping. III. Crop production and profitability

Deep mouldoard ploughing to 0.45 m, gypsum application (5 t ha⁻¹), and double cropping were evaluated, alone and combined, as ameliorants for a hardsetting red-brown earth (Alfisol). The double cropped treatment consisted of winter wheat (Triticum aestivum) and a summer forage crop consisting of sudax (Sorghum sudanese) intersown with cowpea (Vigna unguiculata). This paper describes the effect of these treatments on crop development, yield, crop-water relations and economic returns. Crop emergence was not affected by any treatment. Mouldboard ploughing significantly increased wheat grain yield from 1.86 to 2.15 t ha⁻¹, in 1984, but not in 1985. Yield was also increased from 5.18 to 5.68 t ha⁻¹ as a result of gypsum addition in 1985—after no significant difference in 1984. Double cropping significantly increased yield (by 0.6 t ha⁻¹) in 1985. Greater increases were obtained with the summer cropping phase where higher evaporative demand during the summer would have aggravated soil structural problems associated with hardsetting. Sudax-cowpea dry matter yields were increased from 13.6 to 17 t ha⁻¹ and from 13.0 to 17.5 t ha⁻¹ during summer 1984–1985 by gypsum and mouldboard ploughing, respectively. The higher yields are attributed to increased water storage and depletion, and reduced soil strength for the mouldboard ploughed and gypsum main treatments. No interactions were found between the main treatments, except at the end of the 1985 winter cropping phase when there was a negative interaction between mouldboard ploughing and gypsum application for dry matter, equivalent root length and yield. The yield increases associated with mouldboard ploughing did not persist beyond the second year of the experiment. Double cropping combined with mouldboard ploughing (Mb+DC) was the most profitable treatment, whereas mouldboard ploughing with gypsum (Mb+G) was the least profitable for the crops grown in this experiment.     

Autophagy genes are essential for dauer development and life-span extension in C. elegans

Both dauer formation (a stage of developmental arrest) and adult life-span in Caenorhabditis elegans are negatively regulated by insulin-like signaling, but little is known about cellular pathways that mediate these processes. Autophagy, through the sequestration and delivery of cargo to the lysosomes, is the major route for degrading long-lived proteins and cytoplasmic organelles in eukaryotic cells. Using nematodes with a loss-of-function mutation in the insulin-like signaling pathway, we show that bec-1, the C. elegans ortholog of the yeast and mammalian autophagy gene APG6/VPS30/beclin1, is essential for normal dauer morphogenesis and life-span extension. Dauer formation is associated with increased autophagy and also requires C. elegans orthologs of the yeast autophagy genes APG1, APG7, APG8, and AUT10. Thus, autophagy is a cellular pathway essential for dauer development and life-span extension in C. elegans.     

Phase-coherent optical pulse synthesis from separate femtosecond lasers

We generated a coherently synthesized optical pulse from two independent mode-locked femtosecond lasers, providing a route to extend the coherent bandwidth available for ultrafast science. The two separate lasers (one centered at 760 nanometers wavelength, the other at 810 nanometers) are tightly synchronized and phase-locked. Coherence between the two lasers is demonstrated via spectral interferometry and second-order field cross-correlation. Measurements reveal a coherently synthesized pulse that has a temporally narrower second-order autocorrelation width and that exhibits a larger amplitude than the individual laser outputs. This work represents a new and flexible approach to the synthesis of coherent light.     

Linkage of early-onset familial breast cancer to chromosome 17q21

Human breast cancer is usually caused by genetic alterations of somatic cells of the breast, but occasionally, susceptibility to the disease is inherited. Mapping the genes responsible for inherited breast cancer may also allow the identification of early lesions that are critical for the development of breast cancer in the general population. Chromosome 17q21 appears to be the locale of a gene for inherited susceptibility to breast cancer in families with early-onset disease. Genetic analysis yields a lod score (logarithm of the likelihood ratio for linkage) of 5.98 for linkage of breast cancer susceptibility to D17S74 in early-onset families and negative lod scores in families with late-onset disease. Likelihood ratios in favor of linkage heterogeneity among families ranged between 2000:1 and greater than 10(6):1 on the basis of multipoint analysis of four loci in the region.