Identification of serum differential proteins in colorectal adenoma and early malignantly transformed adenoma by proteomics

AIM: To investigate the associated proteins and sensitive biomarkers for early diagnosis of colorectal adenocarcinoma by comparing the results of differential proteomic analysis between colorectal adenoma and early malignantly transformed adenoma. METHODS: Two-dimensional gel electrophoresis was used to define patterns of protein expressions of colorectal adenoma and early malignantly transformed adenoma. Proteins expressed differentially among groups were detected, cut out and analyzed by MALDI-TOF/TOF mass spectrometry. RESULTS: Two-dimensional protein maps of colorectal adenoma and early malignantly transformed adenoma were analyzed with gel-analysis software, an average of 1 672 spots in adenoma, 1 732 in early malignantly transformed adenoma were observed. 28 spots of a 1.5-fold change were found, including 15 proteins down-regulated and 13 up-regulated in early malignantly transformed adenoma, in which 23 proteins were identified by mass spectrometry, the rate of identification was 82.14%. 13 differential proteins were attained, 8 were up-regulated and 5 were down-regulated, which was classified to 6 categories, including protease inhibitor, complement, immunoglobulin, keratoproteins, signal transduction protein and function-unknown proteins. CONCLUSION: The changes of serum proteins in early malignantly transformed adenoma from adenoma can be identified by proteomic technology. Proteins detected in the study may provide new biomarkers correlated with biological behavior of colorectal adenocarcinoma.     

Relationship between zinc and zinc transporter expression in human lung cancer cells

AIM: To study the changes of zinc transporter gene expression in A-549 cell line exposed to ZnCl2 and N,N,N’,N’-tetrakis 2-pyridylmethyl ethylenediamine (TPEN).METHODS: Human lung cancer cell line A-549 was exposed to different concentrations of ZnCl2 (0, 50, 100, 150, 200 μmol/L) and TPEN (0, 5, 10, 15, 20 μmol/L), respectively. Twelve hours later, the cell viability was measured by MTT (methyl thiazolyl tetrazolium) and levels of zinc transporter mRNA was detected by RT-PCR. Zinquin was used to estimate the intracellular zinc concentrations.RESULTS: A-549 cell viability rate was significantly decreased when exposed to ZnCl2 at concentrations of 150 and 200 μmol/L, and to TPEN. The intracellular zinc concentration was significantly increased when exposed to ZnCl2 and decreased when exposed to TPEN. Zinc transporter (ZnT-1) mRNA level was increased along with the increase in the concentration of ZnCl2 but decreased when exposed to TPEN. The expressions of ZIP-1 and ZIP-10 (Zrt-and Irt-like protein) were increased along with the increase in the concentration of TPEN but decreased when exposed to ZnCl2.CONCLUSION: ZnT-1 expression is induced by zinc supplement. ZIP-1 and ZIP-10 expressions are induced by zinc deficiency and repressed by zinc supplement.     

Regulatory mechanism of sodium-calcium exchanger in hypertrophic myocardium

Myocardium hypertrophy is a compensated response to cardiac disease in which intracellular Ca2+ concentration is elevated, the essential signal that results in hypertrophy. The cardiac sodium-calcium exchanger (NCX) is a bidirectional ion transporter in sarcolemma. The decrease in NCX activity as well as its inverse mode is one of the main mechanisms that contribute to cytosolic Ca2+ loading in hypertrophy. The extracellular signal molecules, the change of Na+ /Ca2+ concentrations and intracellular modulating proteins during cardiac hypertrophy can affect the activity or NCX function, and increase intracellular Ca2+. We summarized the regulatory mechanism of NCX in myocardium hypertrophy.     

胡萝卜新品种杂交一代红参的选育

红参胡萝卜是用完全瓣化型雄性不育系培育的杂交一代新品种.该品种肉质根长圆柱形,韧皮部和木质部均为橙红色,个体整齐度高,春播平均单根质量200.5 g,产量450 002kg/hm2;秋播平均单根质量290.5 g,产量64 500 kg/hm2.β胡萝卜素含量110.0 mg/kg,商品率高,叶幅小,成形早,抗抽薹,适合春、秋种植.     

送春与多花兰杂种的非共生萌发与快速繁殖

用地生兰送春和附生兰多花兰进行种间杂交,对杂交种子进行非共生萌发和快速繁殖研究.结果表明:胚龄7个月、0.1 mol/L KOH处理10 min的种子萌发率最高;种子萌发后原球茎的增殖以Z4 (KC+BA 0.4 mg/L+NAA 0.6 mg/L+KT 0.2 mg/L ) 培养基为最好,增殖系数可达到12.8;在原球茎增殖和分化成苗过程中加入活性碳能够有效地防止切口褐化.     

蝴蝶兰种子无菌播种诱导增殖原球茎试验研究

利用不同激素浓度及添加物对蝴蝶兰未成熟胚有效原球茎进行诱导研究.结果表明:进行无菌播种采收未开裂的果荚比已开裂的要有利的多,采摘最佳时间为授粉后120 d;原球茎增殖效果最佳的激素组合是BA 3.0+NAA 0.2;200 mL/L椰乳对蝴蝶兰PLB增殖的影响效果最好,其次为香蕉和马铃薯.     

盆栽竹芋的养护要点及老苗快速复壮技术

针对竹芋对环境因子敏感、栽培技术要求高,易出现叶片生理病害及叶斑病、根茎腐病等病害的情况,介绍了竹芋小苗、大苗期盆栽不同生长时期栽培管理的要点.针对盆花养护与栽培中出现的秋季、冬春季普遍存在大量失去美感的盆栽竹芋老苗,通过大量养护复壮工作,总结了秋季盆栽竹芋适当重剪、夏季高温后盆栽加强遮荫与叶面喷水、长期室内摆放后的老苗适当增光控水迅速复壮技术;春季分盆换土、保湿、保温促新芽萌发的低温冬季后的竹芋盆花彻底复壮技术.     

甜瓜花粉离体萌发条件优化试验

以3种不同类型的甜瓜新鲜花粉为试材,采用了正交试验L9(34)研究供体基因型、萌发温度、蔗糖浓度和硼酸浓度对花粉萌发和伸长的影响,建立了甜瓜花粉离体萌发适宜条件.试验结果表明,温度对花粉萌发和生长影响最大,硼酸浓度次之,基因型影响最小.甜瓜花粉离体萌发适宜条件为:温度25℃、15%蔗糖、0.4 g/L硼酸.     

食用菌生物降解木质素的研究现状

木质素是农作物秸秆中的主要成份之一,木质素降解直接影响秸秆等植物资源的利用效率.从降解木质素的食用菌种类、食用菌木质素降解酶系及其营养调控机理、应用前景共4个方面,综述了食用菌生物降解秸秆木质素的研究现状.     

攀西地区番石榴引种观察

番石榴（Psidium guajavaLinn）别名番稔、芭乐、鸡屎果,是亚热带名优水果之一,生长快、产量高。现台湾、广东、广西、福建、江西等省均有栽培,其果实含有丰富的维生素,铁、钙、磷的含量也极高,适合鲜食、加工,便于贮藏和运输。其果肉具有降压、祛痰润肺、消食解酒、降火利尿等作用,中外民间医药多有采用番石榴果实及其叶子治疗糖尿病。番石榴营养丰富,维生素C含量高。