Influence of non-cellulose structural carbohydrate composition on plant material decomposition in soil

The C mineralisation pattern during the early stage of decomposition of plant materials is largely determined by their content of different carbohydrates. This study investigated whether detailed plant analysis could provide a better prediction of C mineralisation during decomposition than proximate analysis [neutral detergent solution (NDF)/acid detergent solution (ADF)]. The detailed analysis included sugars, fructans, starch, pectin, cellulose, lignin and organic N. To determine whether differences in decomposition rate were related to differences in hemicellulose composition, the analysis particularly emphasised the concentrations of arabinose and xylose in hemicelluloses. Carbon dioxide evolution was monitored hourly in soil amended with ten different plant materials. Principal component and regression analysis showed that C mineralisation during day 1 was closely related to free sugars, fructans and soluble organic N components (R ² = 0.83). The sum of non-cellulose structural carbohydrates (intermediate NDF/ADF fraction) was not related to C mineralisation between days 1 and 9. In contrast, a model including starch and protein in addition to the non-cellulose structural carbohydrates, with the hemicelluloses replaced by arabinose and xylose, showed a strong relationship with evolved CO₂ (R ² = 0.87). Carbon mineralisation between days 9 and 34 was better explained by xylan, cellulose and lignin (R ² = 0.72) than by lignocellulose in the ADF fraction. Our results indicated that proximate analyses were not sufficient to explain differences in decomposition. To predict C mineralisation from the range of plant materials studied, we propose a minimum set of analyses comprising total N, free sugars, starch, arabinose, xylan, cellulose and lignin.     

Leaching characteristics of homologues of benzalkonium chloride from wood treated with ammoniacal copper quaternary wood preservative

In Japan, ammoniacal copper quaternary wood preservatives type-1 (ACQ-1), which contains copper and benzalkonium chloride as its active ingredients, is among the most widely used wood preservatives in the pressure treatment of wood. Benzalkonium chloride (BAC) in commercial ACQ-1 products mainly comprises C12 and C14 homologues. In the present study, the leaching characteristics of these BAC homologues were investigated using the heartwood and sapwood portions of Japanese cedar, Japanese larch, and Sakhalin fir treated with ACQ-1 and 1% monoethanolamine (MEA) solution containing equimolar amounts of homologues. Distilled water (DW) and artificial seawater (SW) were used as leaching media. Consequently, it was observed that the leaching rate of the C12 homologue tended to be higher than that of the C14 homologue in DW. The leaching of C12 homologues was accelerated by using SW, resulting in a significantly higher leaching rate than the C14 homologue using SW. It was thought that the difference in the hydrophobicities based on alkyl chain lengths resulted in these phenomena. However, when the heartwood portion of Japanese larch was treated with the homologues in MEA, the leaching rate of the C14 homologue was significantly higher than that of the C12 homologue.     

UV老化后氧化铁颜料着色木纤维-HDPE复合材料的性能变化

探讨了氧化铁颜料对木纤维．高密度聚乙烯复合材耐老化性能的改善作用。采用四种常用的氧化铁颜料与木纤维、高密度聚乙烯和其他加工助剂干混,并用自行设计的双螺杆／单螺杆双阶挤出机组制造木塑复合材料。对该木塑复合材料进行人工加速紫外循环老化处理,用CIE1976L^＊a^＊b^＊表色体系和ASTMD790标准分别对老化前后的材料进行测试,结果显示加入颜料以后木塑复合材料的抗弯弹性模量没有明显的变化,但是弯曲强度都有一定程度的提高。经过2000h人工加速紫外老化以后,不论是颜色要是力学性能都发生了明显的变化。铁红和铁黑着色的试样在整个老化过程中表现较好,颜料添加量约2．28％比较适宜。     

Water absorption of boron-treated and heat-modified wood

Heat treatments change the chemical and physical properties of wood and dimensional stability and hygroscopicity are affected as a result of modifications of wood cell components. This study evaluated the water absorption of wood specimens treated with boron compounds followed by heat treatment. Sugi (Cryptomeria japonica D. Don) sapwood specimens treated with either boric acid (BA) or disodium octoborate tetrahydrate (DOT) solutions were heat-modified at either 180° or 220°C for 2 or 4 h. Carbohydrate composition and water absorption of the specimens were then measured and compared with those of untreated and unheated specimens. Wood carbohydrates were significantly degraded in the specimens after heat treatment. The heat treatment evidently decreased the water absorption and the heat-modified specimens absorbed less water than unheated specimens. The higher the treatment temperature and the longer the treatment time, the lower the amount of absorbed water. The boron-treated and heat-modified specimens, however, showed increased water absorption due to the hygroscopic properties of BA and DOT.     

Inheritance and detoxification enzyme levels in <Emphasis Type="Italic">Tetranychus urticae</Emphasis> Koch (Acari: Tetranychidae) strain selected with chlorpyrifos

Changes in insecticide susceptibilities and detoxifying enzyme activities were measured in a strain of Tetranychus urticae Koch following repeated exposure to the organophosphate insecticide, chlorpyrifos. Twelve consecutive selection at the LC₆₀ of the parental strain increased resistance from 8.58 to 91.45 fold. The interaction of some synergists [piperonyl butoxide, triphenyl phosphate and S-benzyl-O,O-diisopropyl phosphorothioate (IBP)] with chlorpyrifos was analyzed in the selected strain. Solely IBP showed a low synergistic effect with chlorpyrifos. The selected strain also demonstrated resistance against abamectin, propargite, clofentezine and fenpyroximate. The mode of resistance inheritance to chlorpyrifos was found to be incompletely dominant, and not sex-linked. Non-specific esterase enzyme activity was raised from 19.35 to 33.59 mOD/min/mg proteins during the selection period and it was observed that esterase band intensities visualized by polyacrylamide gel electrophoresis increased. This study has investigated the selection of resistance to chlorpyrifos and documented resistance to abamectin, propargite, clofentezine and fenpyroximate in Turkish T. urticae. Esterase enzymes may be playing a role in chlorpyrifos resistance while glutathione S-transferase (GST) and P450 enzymes do not appear to have any significant involvement.     

Effects of different supplements on tetraploid black locust (<Emphasis Type="Italic">Robinia pseudoacacia</Emphasis> L.) silage

Preparation of silage is a common method to preserve green forage. It plays an important role in improving forage utilization,     

<Emphasis Type="Italic">Lupinus albus</Emphasis> Conglutin Gamma Modifies the Gene Expressions of Enzymes Involved in Glucose Hepatic Production <Emphasis Type="Italic">In Vivo</Emphasis>

Lupinus albus seeds contain conglutin gamma (Cγ) protein, which exerts a hypoglycemic effect and positively modifies proteins involved in glucose homeostasis. Cγ could potentially be used to manage patients with impaired glucose metabolism, but there remains a need to evaluate its effects on hepatic glucose production. The present study aimed to analyze G6pc, Fbp1, and Pck1 gene expressions in two experimental animal models of impaired glucose metabolism. We also evaluated hepatic and renal tissue integrity following Cγ treatment. To generate an insulin resistance model, male Wistar rats were provided 30% sucrose solution ad libitum for 20 weeks. To generate a type 2 diabetes model (STZ), five-day-old rats were intraperitoneally injected with streptozotocin (150 mg/kg). Each animal model was randomized into three subgroups that received the following oral treatments daily for one week: 0.9% w/v NaCl (vehicle; IR-Ctrl and STZ-Ctrl); metformin 300 mg/kg (IR-Met and STZ-Met); and Cγ 150 mg/kg (IR-Cγ and STZ-Cγ). Biochemical parameters were assessed pre- and post-treatment using colorimetric or enzymatic methods. We also performed histological analysis of hepatic and renal tissue. G6pc, Fbp1, and Pck1 gene expressions were quantified using real-time PCR. No histological changes were observed in any group. Post-treatment G6pc gene expression was decreased in the IR-Cγ and STZ-Cγ groups. Post-treatment Fbp1 and Pck1 gene expressions were reduced in the IR-Cγ group but increased in STZ-Cγ animals. Overall, these findings suggest that Cγ is involved in reducing hepatic glucose production, mainly through G6pc inhibition in impaired glucose metabolism disorders.     

Some Nutritional Characteristics of Enzymatically Resistant Maltodextrin from Cassava (<Emphasis Type="Italic">Manihot esculenta</Emphasis> Crantz) Starch

Cassava (Manihot esculenta Crantz) native starch was treated with pyroconversion and enzymatic hydrolysis to produce a pyrodextrin and an enzyme-resistant maltodextrin. Some nutritional characteristics were quantified for both compounds. Pyroconversion was done using a 160:1 (p/v) starch:HCl ratio, 90 °C temperature and 3 h reaction time. The resulting pyrodextrin contained 46.21% indigestible starch and 78.86% dietary fiber. Thermostable α-amylase (0.01%) was used to hydrolyze the pyrodextrin at 95 °C for 5 min. The resulting resistant maltodextrin contained 24.45% dextrose equivalents, 56.06% indigestible starch and 86.62% dietary fiber. Compared to the cassava native starch, the pyrodextrin exhibited 56% solubility at room temperature and the resistant maltodextrin 100%. The glycemic index value for the resistant maltodextrin was 59% in healthy persons. Its high indigestible starch and dietary fiber contents, as well as its complete solubility, make the resistant maltodextrin a promising ingredient for raising dietary fiber content in a wide range of foods, especially in drinks, dairy products, creams and soups.     

Vacuum Drying for Extending Litchi Shelf-Life: Vitamin C,Total Phenolics,Texture and Shelf-Life Assessment

In an attempt to obtain shelf-stable litchi fruit with preserved nutritional quality and good sensory features, quarters of peeled and pitted fruits were vacuum dried at 50, 60 and 70 °C at a constant pressure of 8.0 kPa. The product was assessed for its vitamin C, total phenolics and texture (hardness). In addition, the product with the best texture was assessed for its shelf-life by means of accelerated testing. Results suggest that vacuum dried litchi retained almost 70% of the vitamin C and total phenolics when compared to frozen fruits (control). Vitamin C and phenolic compounds content significantly decreased with drying, while no difference was found between different drying temperatures. Hardness increased with drying temperature. The sample dried at 70 °C presented crispness, which is a desired quality feature in dried fruit products. This sample was subjected to shelf-life evaluation, whose result suggests a shelf-life of eight months at 23 °C. Total color change (CIE ΔE₀₀) was the expiry criterion. Vacuum drying was a suitable technique for producing shelf-stable litchi fruit with good texture while preserving its desirable original nutrients. Consumption of vacuum dried litchi may be beneficial to health due to its remarkable content of phenolic compounds and vitamin C.     

Antioxidant Capacity of Beetroot: Traditional vs Novel Approaches

Red beetroot has been ranked among the 10 most potent antioxidant vegetables, although only extraction-based methods have been used to evaluate its total antioxidant capacity. Therefore, the present study aims at comparing the traditional extraction-based method with two more recent approaches (QUENCHER -QUick, Easy, New, CHEap and Reproducible- and GAR -global antioxidant response method), in order to establish their suitability in the case of beetroot. Our results indicate that the total antioxidant capacity of beetroot would be underestimated when using extraction-based procedures, since both QUENCHER and GAR methods resulted in a higher total antioxidant capacity. The effect of a thermal treatment on the total antioxidant capacity of beetroot varies among the methods evaluated and our findings suggest different compounds responsible for the total antioxidant capacity detected in each pre-processing method. Remarkably, the present study demonstrates that the traditional extraction-based method seems useful to screen for (changes in) the “bioavailable” antioxidant potential of the root.