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561.
Bliss S Todhunter RJ Quaas R Casella G Wu R Lust G Williams AJ Hamilton S Dykes NL Yeager A Gilbert RO Burton-Wurster NI Acland GM 《American journal of veterinary research》2002,63(7):1029-1035
OBJECTIVE: To determine the genetic influence on expression of traits associated with canine hip dysplasia. ANIMALS: 193 dogs from an experimental canine pedigree. PROCEDURE: An experimental canine pedigree was developed for linkage analysis of hip dysplasia by mating dysplastic Labrador Retrievers with nondysplastic Greyhounds. A statistical model was designed to test the effects of Labrador Retriever and Greyhound alleles on age at detection of femoral capital epiphyseal ossification, 8-month distraction index, and 8-month dorsolateral subluxation score. RESULTS: The additive effect was significant for age at detection of femoral capital epiphyseal ossification. Restricted maximum likelihood estimates (+/-SD) for this trait were 6.4+/-1.95, 10.2+/-2.0, 10.8+/-3.1, 11.4+/-2.1, and 13.6+/-4.6 days of age for Greyhounds, Greyhound backcross dogs, F1 dogs, Labrador Retriever backcross dogs, and Labrador Retrievers, respectively. The additive effect was also significant for the distraction index. Estimates for this trait were 0.21+/-0.07, 0.29+/-0.15, 0.44+/-0.12, 0.52+/-0.18, and 0.6+/-0.17 for the same groups, respectively. For the dorsolateral subluxation score, additive and dominance effects were significant. Estimates for this trait were 73.5+/-4.1, 71.3+/-6.5, 69.1+/-6.0, 50.6+/-12.9, and 48.4+/-7.7%, respectively, for the same groups. CONCLUSIONS: In this canine pedigree, traits associated with canine hip dysplasia are heritable. Phenotypic differences exist among founder dogs of each breed and their crosses. This pedigree should be useful for identification of quantitative trait loci underlying the dysplastic phenotype. 相似文献
562.
Gilbert D. McCollum 《American Journal of Potato Research》1977,54(12):593-602
Only slight reductions were observed in sprouting and growth from irradiated tuber eyes of ‘Katahdin” and B5141-6 potatoes at irradiation doses below 4500 rads X-rays or 1200 rads fast neutrons. Survival was reduced about 50% by 1400 rads fast neutrons and 97% by 5500 rads X-rays. Visible mutants and pollen steriles were rare at all doses used. A small number of subclones of irradiated ‘Katahdin’ having greater than normal pollen abortion were selected in an effort to locate irradiationinduced chromosome mutations. 相似文献
563.
564.
Yeung T Gilbert GE Shi J Silvius J Kapus A Grinstein S 《Science (New York, N.Y.)》2008,319(5860):210-213
Electrostatic interactions with negatively charged membranes contribute to the subcellular targeting of proteins with polybasic clusters or cationic domains. Although the anionic phospholipid phosphatidylserine is comparatively abundant, its contribution to the surface charge of individual cellular membranes is unknown, partly because of the lack of reagents to analyze its distribution in intact cells. We developed a biosensor to study the subcellular distribution of phosphatidylserine and found that it binds the cytosolic leaflets of the plasma membrane, as well as endosomes and lysosomes. The negative charge associated with the presence of phosphatidylserine directed proteins with moderately positive charge to the endocytic pathway. More strongly cationic proteins, normally associated with the plasma membrane, relocalized to endocytic compartments when the plasma membrane surface charge decreased on calcium influx. 相似文献