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991.
Overview of Mechanisms and Uses of Trichoderma spp 总被引:1,自引:0,他引:1
Harman GE 《Phytopathology》2006,96(2):190-194
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Marije Risselada DVM PhD Diplomate ECVS Gary W. Ellison DVM MS Diplomate ACVS Maximilian M.R. Polyak M Phil Jim Van Gilder BS Kristin Kirkby MS DVM Diplomate ACVS Stanley E. Kim BVSc MS 《Veterinary surgery : VS》2010,39(7):856-862
Objective: To compare surgical time and intraoperative blood loss for 5 partial liver lobectomy techniques in the dog. Study Design: Experimental in vivo study. Animals: Dogs (n=10). Methods: Five surgical techniques (SurgiTie?; LigaSure?; Ultracision® Harmonic Scalpel [UAS]; Suction+Clip; Suction+thoracoabdominal stapler [TA]) for partial liver lobectomy in dogs were evaluated and compared for total surgical time and intraoperative blood loss. Body weight, activated clotting time (ACT), heart rate, and intraoperative blood pressure (BP) were recorded. Blood loss was determined by adding the weight of the blood soaked sponges during surgery (1 g=1 mL) to the amount of suctioned blood (mL). Surgical time (in seconds) was determined from the start of the lobectomy until cessation of bleeding from the stump. Mean surgical time and mean blood loss for each technique were compared using a Tukey's multiple comparison test. Results: No significant differences were found between dogs for weight, ACT, heart rate, and intraoperative BP. No complications were seen with the SurgiTie? technique in 9 of 10 cases. There was no significant difference in surgical time between techniques however there was a significant difference for blood loss; the Suction+Clip method had significantly more blood loss than the other techniques. Conclusions: Skeletonization of the lobar vessels before individually clipping them (Suction+Clip) resulted in a higher blood loss than using Suction+TA, UAS, SurgiTie? or the LigaSure? device. The SurgiTie? appears to be an acceptable method for partial liver lobectomy. Clinical Relevance: Although skeletonization and individually clipping the vessels had the highest blood loss, it still was <7.5% of total blood volume. All 5 techniques should be safe for clinical use in small to medium sized dogs up to 26 kg. 相似文献
996.
Effects of oxygen-enriched nutrient solution on greenhouse cucumber and pepper production 总被引:1,自引:0,他引:1
David L. Ehret Diane Edwards Tom Helmer Wei Lin Gary Jones Martine Dorais Athanasios P. Papadopoulos 《Scientia Horticulturae》2010
A series of experiments were conducted with greenhouse cucumber and pepper plants to determine the effects of oxygen enrichment of the irrigation water on yield and fruit shelf-life. The experiments were carried out in soilless culture in research greenhouses. Depending on the experiment, treatments included sub-ambient (2 mg L−1), ambient (5–6 mg L−1), medium (16 mg L−1) and high (30–40 mg L−1) levels of oxygen in the supply tank. Cucumber plants were grown in yellow cedar sawdust and pepper plants in either sawdust or perlite. Oxygen enrichment resulted in a promotion of cucumber yield in only one experiment; in two other experiments, none of the oxygen treatments, including those at sub-ambient levels, had an effect. There were no effects of oxygen enrichment on pepper yield. However, in both cucumber and pepper, fruit shelf-life was extended in oxygen-enriched treatments. In terms of system efficacy, oxygen levels in the irrigation water were measured at the dripper and found to decrease by 20–67% of initial values compared to the supply tank values, depending on the initial oxygen concentration and on the experiment. Oxygen concentrations decreased even further to virtually ambient levels when measured in the drain water or in the substrate reservoir. Cucumber plant growth was promoted under conditions which facilitated consistently high oxygen in the root zone, achieved through heavy irrigation (1 min in two) with oxygen-enriched nutrient solution of plants grown in saturated substrate (pumice). However, those extreme irrigation rates would not be practical for commercial cucumber or pepper production. Overall, this study demonstrates that oxygen enrichment of porous substrates under typical hydroponic conditions is difficult and possibly because of this, effects on yield are infrequent. However, fruit shelf-life may be improved. 相似文献
997.
PRACTICAL RELEVANCE: Cognitive dysfunction syndrome (CDS) is a widely accepted diagnosis in dogs, with established treatment options. In cats, however, our understanding of cognitive dysfunction is still being shaped by ongoing research in the field, and limited treatment options are available. Recent clinical studies indicate that old age in the cat is accompanied by increased behavioural signs such as wandering, vocalization and night-time activity that are not attributable to identifiable medical problems. It is essential, therefore, that veterinarians include behavioural well-being in the routine care of senior cats. PATIENT GROUP: While the exact age of onset is not established, studies suggest that age-related behavioural changes consistent with cognitive dysfunction are prevalent in cats as early as 10 years of age and that prevalence increases significantly in older cats. CLINICAL CHALLENGES: The diagnosis of cognitive dysfunction requires the identification of geriatric behavioural changes that are not caused by other medical problems, although the two may not be mutually exclusive. Therefore, the practitioner must rely heavily on owner reports and history to ensure prompt diagnosis and treatment. The absence of any approved dietary or pharmaceutical interventions for cognitive dysfunction adds a further challenge, although several possibilities exist. EVIDENCE BASE: This article draws on recent research that has produced neuropathological, cognitive and behavioural evidence for cognitive dysfunction in aging cats. As an impetus to further our understanding of this disease and potential treatment options, the authors propose a behavioural checklist that might aid in the clinical diagnosis of feline CDS and discuss treatment options that have proven successful in the canine counterpart of this disease. 相似文献
998.
An integrated genomic analysis of human glioblastoma multiforme 总被引:1,自引:0,他引:1
Parsons DW Jones S Zhang X Lin JC Leary RJ Angenendt P Mankoo P Carter H Siu IM Gallia GL Olivi A McLendon R Rasheed BA Keir S Nikolskaya T Nikolsky Y Busam DA Tekleab H Diaz LA Hartigan J Smith DR Strausberg RL Marie SK Shinjo SM Yan H Riggins GJ Bigner DD Karchin R Papadopoulos N Parmigiani G Vogelstein B Velculescu VE Kinzler KW 《Science (New York, N.Y.)》2008,321(5897):1807-1812
Glioblastoma multiforme (GBM) is the most common and lethal type of brain cancer. To identify the genetic alterations in GBMs, we sequenced 20,661 protein coding genes, determined the presence of amplifications and deletions using high-density oligonucleotide arrays, and performed gene expression analyses using next-generation sequencing technologies in 22 human tumor samples. This comprehensive analysis led to the discovery of a variety of genes that were not known to be altered in GBMs. Most notably, we found recurrent mutations in the active site of isocitrate dehydrogenase 1 (IDH1) in 12% of GBM patients. Mutations in IDH1 occurred in a large fraction of young patients and in most patients with secondary GBMs and were associated with an increase in overall survival. These studies demonstrate the value of unbiased genomic analyses in the characterization of human brain cancer and identify a potentially useful genetic alteration for the classification and targeted therapy of GBMs. 相似文献
999.
Waldo GL Ricks TK Hicks SN Cheever ML Kawano T Tsuboi K Wang X Montell C Kozasa T Sondek J Harden TK 《Science (New York, N.Y.)》2010,330(6006):974-980
Transmembrane signals initiated by a broad range of extracellular stimuli converge on nodes that regulate phospholipase C (PLC)-dependent inositol lipid hydrolysis for signal propagation. We describe how heterotrimeric guanine nucleotide-binding proteins (G proteins) activate PLC-βs and in turn are deactivated by these downstream effectors. The 2.7-angstrom structure of PLC-β3 bound to activated Gα(q) reveals a conserved module found within PLC-βs and other effectors optimized for rapid engagement of activated G proteins. The active site of PLC-β3 in the complex is occluded by an intramolecular plug that is likely removed upon G protein-dependent anchoring and orientation of the lipase at membrane surfaces. A second domain of PLC-β3 subsequently accelerates guanosine triphosphate hydrolysis by Gα(q), causing the complex to dissociate and terminate signal propagation. Mutations within this domain dramatically delay signal termination in vitro and in vivo. Consequently, this work suggests a dynamic catch-and-release mechanism used to sharpen spatiotemporal signals mediated by diverse sensory inputs. 相似文献
1000.
Li W Shi Z Yu M Ren W Smith C Epstein JH Wang H Crameri G Hu Z Zhang H Zhang J McEachern J Field H Daszak P Eaton BT Zhang S Wang LF 《Science (New York, N.Y.)》2005,310(5748):676-679
Severe acute respiratory syndrome (SARS) emerged in 2002 to 2003 in southern China. The origin of its etiological agent, the SARS coronavirus (SARS-CoV), remains elusive. Here we report that species of bats are a natural host of coronaviruses closely related to those responsible for the SARS outbreak. These viruses, termed SARS-like coronaviruses (SL-CoVs), display greater genetic variation than SARS-CoV isolated from humans or from civets. The human and civet isolates of SARS-CoV nestle phylogenetically within the spectrum of SL-CoVs, indicating that the virus responsible for the SARS outbreak was a member of this coronavirus group. 相似文献