全文获取类型
收费全文 | 13928篇 |
免费 | 695篇 |
国内免费 | 1680篇 |
专业分类
林业 | 1496篇 |
农学 | 2146篇 |
基础科学 | 1249篇 |
2238篇 | |
综合类 | 4360篇 |
农作物 | 692篇 |
水产渔业 | 496篇 |
畜牧兽医 | 1969篇 |
园艺 | 611篇 |
植物保护 | 1046篇 |
出版年
2024年 | 64篇 |
2023年 | 223篇 |
2022年 | 469篇 |
2021年 | 681篇 |
2020年 | 603篇 |
2019年 | 536篇 |
2018年 | 415篇 |
2017年 | 572篇 |
2016年 | 496篇 |
2015年 | 697篇 |
2014年 | 678篇 |
2013年 | 775篇 |
2012年 | 974篇 |
2011年 | 988篇 |
2010年 | 856篇 |
2009年 | 804篇 |
2008年 | 781篇 |
2007年 | 755篇 |
2006年 | 684篇 |
2005年 | 680篇 |
2004年 | 299篇 |
2003年 | 266篇 |
2002年 | 244篇 |
2001年 | 230篇 |
2000年 | 293篇 |
1999年 | 309篇 |
1998年 | 264篇 |
1997年 | 267篇 |
1996年 | 215篇 |
1995年 | 221篇 |
1994年 | 205篇 |
1993年 | 155篇 |
1992年 | 139篇 |
1991年 | 104篇 |
1990年 | 93篇 |
1989年 | 81篇 |
1988年 | 68篇 |
1987年 | 41篇 |
1986年 | 23篇 |
1985年 | 9篇 |
1984年 | 8篇 |
1983年 | 9篇 |
1982年 | 8篇 |
1981年 | 14篇 |
1980年 | 5篇 |
1979年 | 1篇 |
1965年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
51.
52.
产蛋下降综合征(EDS-76)病毒DNA探针的制备及应用研究 总被引:2,自引:0,他引:2
用纯化的产蛋下降综合征(EDS-76)病毒(H91毒株)悬液提取的核酸,经琼脂糖凝胶电泳只出现1条分子量较大、背景清晰的核酸带。用BamHI酶消化产生4个片段,大小分别为17kb、10kb、4.0kb和2.0kb。将其中的2.0kb片段克隆进pUC18DNA载体中,重组质粒DNA用digoxigenin标记作为DNA探针,在dotblot中该探针不与鹅胚尿囊液核酸抽提物、马立克氏病病毒(MDV)DNA、鸡传染性贫血病毒(CAV)DNA、SPF鸡基因组DNA等核酸反应,只与3株EDS病毒(EDS标准毒株AV-127、EDSH91毒株和从健康鹅体中分离的EDSY81毒株)DNA呈阳性反应。人工感染产蛋母鸡和雏鸡后24h即可用该探针从泄殖腔棉拭子样品中检测出EDS病毒DNA,在感染后35h仍能从部分感染鸡样品中检出。对部分探针检测阳性鸡的泄殖腔棉拭子样品用SPF鸡胚分离病毒,并用HA和HI试验检测分离病毒的特异性;在感染过程中,同时检测了血清中HI抗体的消长情况。结果表明,人工感染鸡排毒至少可以维持2个月左右,而且血清中HI抗体即使很高,也不能阻止感染鸡的排毒。 相似文献
53.
黄嘌呤氧化酶传感器的研制及其对鱼鲜度的检测 总被引:2,自引:0,他引:2
将黄嘌呤氧化酶以共价连接法固定于二醋酸纤维素薄膜上,制成的酶膜与极谱式氧电极共同组成对次黄嘌呤进行定量测定的黄嘌呤氧化酶传感器。该传感器测定范围为10~120mg/L,响应时间10s,不同浓度次黄嘌呤标准溶液对传感器10s响应值的相关系数为γ=-0.9966,次黄嘌呤标准溶液重复测定60次(10s响应值)的变异系数为CV=0.303%。应用该传感器与分光光度法对鱼体次黄嘌呤含量进行了测定比较,二者具有良好的相关性。 相似文献
54.
为研制均匀、稳定的猪瘟病毒(CSFV)核酸标准物质,选择CSFV C株接种于PK-15细胞并收获病毒液,然后经过病毒灭活、分装、冻干,最终形成核酸标准物质。用微滴式数字PCR对制备的CSFV标准物质进行均匀性和稳定性评估、标准物质合作定值,在评定不确定度后计算得出最终量值结果,并开展临床试用。结果显示,该标准物质均匀,4 ℃条件下可稳定存放4周,25 ℃可稳定存放1周,-20 ℃可稳定存放11个月,量值结果为(5.1±0.7)×105 copies/mg;临床试用显示,所制备的标准物质与临床样本的互通性良好。本研究制备的CSFV C株国家核酸标准物质均一性良好、稳定、量值准确度高,并通过了全国标准物质管理委员会评定,可用于动物及动物产品质量安全检测,从而有效保障猪瘟防治工作的开展。 相似文献
55.
在进行2批猪瘟活疫苗(脾淋源)效力检验时,出现效力检验家兔突然死亡现象,为了查明家兔死亡原因,采用无菌检验、支原体检验、血凝试验、兔体中和试验、酶联免疫吸附试验(ELISA)对疫苗或注苗后死亡家兔肝脏、脾脏混合病料进行了检测。结果显示,疫苗的无菌检验、支原体检验结果均为阴性;疫苗及注射疫苗死亡家兔肝脏、脾脏混合病料具有较低的血凝价,血凝试验结果均为可疑;在兔体中和试验中,中和组家兔2/2健康存活,未中和的疫苗对照组家兔2/2死亡;疫苗及注射疫苗死亡后家兔肝脏、脾脏混合病料的ELISA检测结果均为阳性。检测结果证实疫苗中含有兔出血症病毒(Rabbit Haemorrhagic Disease Virus,RHDV)。猪瘟活疫苗(脾淋源)污染RHDV的现象启示:应该加强猪瘟活疫苗(脾淋源)抗原制备过程的控制;同时有必要对猪瘟活疫苗(脾淋源)质量标准进行修订使之进一步补充完善。 相似文献
56.
57.
58.
The limited space in farrowing crate imposes many challenges, such as prolonged farrowing duration and high piglet stillbirth rate. Although the features of farrowing pens compensate for the drawbacks of farrowing crates, they are associated with high piglet crushing mortality caused by the greater space afforded to sows and their rolling-over behaviour. Therefore, a freedom farrowing pen was designed to overcome the drawbacks of both farrowing crates and farrowing pens. The main features of the freedom farrowing pen are its left anti-crushing bar and detachable right anti-crushing bar on the sides of the sow lying area. It also has a 10 cm-high anti-crushing bar in the non-lying area. Eighteen healthy, multiparous Yorkshire sows (3-7 parity) were averaged and randomly assigned to farrowing crates, farrowing pens, and freedom farrowing pens to compare the effects of the farrowing systems on sow behaviour and performance. Results showed that the farrowing duration and the mean piglet birth intervals were longer for the sows in farrowing crates than for those in farrowing pens and freedom farrowing pens (P<0.05), but there was no difference between the sows in farrowing pens and those in freedom farrowing pens (P>0.05). The piglet stillbirth rate was higher for the sows in farrowing crates than for those in farrowing pens and freedom farrowing pens (P<0.001). Crushing mortality was higher among piglets in farrowing pens (P<0.001), but there was no difference between piglets in freedom farrowing pens and those in farrowing crates (P>0.05). The freedom farrowing pen and the farrowing pen allowed sows to turn around and move freely, but because of the different structures of their anti-crushing bars, the increase in sow movement did not cause higher piglet crushing mortality (P>0.05). Sows in freedom farrowing pens were found to be more protective of their piglets. 相似文献
59.
Wang Y Bai Y Qu Q Xu J Chen Y Zhong Z Qiu Y Wang T Du X Wang Z Yu S Fu S Yuan J Zhen Q Yu Y Chen Z Huang L 《Veterinary microbiology》2011,151(3-4):354-362
Brucellosis brings great economic burdens for developing countries. Live attenuated vaccines are the most efficient means for prevention and control of animal Brucellosis. However, the difficulties of differentiating of infection from vaccine immunization, which is essential for eradication programs, limit their applications. Therefore, the development of a vaccine that could differentiate infection from immunization will overcome the limitations and get extensive application. VjbR is a quorum sensing regulator involving in Brucella's intracellular survival. The vjbR∷Tn5 mutants have been proven effective against wild type strain challenge, implying its possibility of use in vaccine candidate development. To further evaluate this candidate gene, in the present study, the antigenicity of purified recombinant VjbR protein was analyzed. Antibodies to Brucella melitensis VjbR could be detected in sera from patients and animals with brucellosis but not in control ones, implying the potential use of this protein as a diagnostic antigen. Then a vjbR mutant of B. melitensis 16M was constructed by replacing the vjbR with kanamycin gene. The mutant showed reduced survival in macrophage and mice. Vaccination of BALB/c mice with 16MΔvjbR conferred significant protective immunity against B. melitensis strain 16M challenges, being equivalent to which induced by the license vaccine Rev.1. The vjbR deletion mutant elicited an anti-Brucella-specific immunoglobulin G response and induced the secretion of gamma interferon and interleukin-10. The most importance is that, the use of vjbR mutants as vaccines in association with diagnostic tests based on the VjbR antigen would allow the serological differentiation between infected and vaccinated animals. These results suggest that 16MΔvjbR is an ideal live attenuated vaccine candidate against B. melitensis and deserves further evaluation for vaccine development. 相似文献
60.