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61.
The objective of this study was to determine if 1) the full employment-unemployment rate, or natural unemployment rate, changed between 1954-79 differentially for various subgroups in the US population; 2) minimum wage laws and unemployment compensation impacted differentially on subgroups in the population; and 3) there were structural shifts in the determinants of unemployment and labor force participation rates among subgroups. The 6 subgroups investigated were white and nonwhite teenagers, white and nonwhite females, and white and nonwhite males. Trends and cycles in unemployment were analyzed using regression techniques and basic time series models, and structural changes in the unemployment rate were analyzed by using a technique developed by Brown, Durbin, and Evans to test for change in estimated regression coefficients. Results indicated that the natural unemployment rate in the US increased from 4.70% to 5.14% between 1959-79. This increase was due in part to the unemployment rate increases observed among different subgroups in the population, and expecially among teenagers. In 1979 the unemployment rates among teenagers were 13.6% for whites and 28.72% for nonwhites. Respective rates in 1979 for white and nonwhite adult females were 4.20% and 9.98%, and for white and nonwhite adult males they were 2.78% and 6.36%. Other findings were 1) increases in minimum wage had a positive impact on the nonwhite teenagers' jobless rates, no effect on the white teenager jobless rate, and a negative impact on the adult unemployment rate; 2) increased unemployment compensation was positively associated with higher jobless rates for adult males and nonwhite teenagers; 3) the jobless rate was not significantly related to changes between 1954-79 in the proportion of different age, sex, and race groups in the population; and 4) structural shifts in the determinants of unemployment were observed for secondary workers only. Tables provided the results of the regression analysis, estimates of unemployment rates, by race, sex, and age for 1959, 1969, and 1979, and labor force composition and employment rates by race, sex, and age for 1954 and 1981. 相似文献
62.
The isotopic investigation of vanillin has been extended to the new sources of natural precursors of vanillin recently introduced with a view of obtaining natural vanillin by biotechnological processes. To check the consistency of the isotopic composition of vanillin with that of the corresponding aromatic fragment, a selective degradation reaction into guaiacol was carried out. The reaction was shown to proceed without significant isotopic fractionation at the sites of interest, and an optimized procedure was defined from the results of an experimental design involving the quantity of reagent and the temperature and duration of the experiment. Guaiacol, which can be easily obtained in a reasonable time, is an interesting isotopic probe for carbon- and oxygen-isotope ratio mass spectrometry (IRMS) determinations. It provides (13)C information specific to the aromatic fragment and, combined with delta(13)C values measured on vanillin itself, it improves the authentication potential of carbon-IRMS. Thus, the natural status of ferulic acid may be characterized by significant (13)C depletion at the formyl site. Similarly, the oxygen-18 content of guaiacol is a better authentication tool than delta(18)O of vanillin because it does not suffer the drawback of being altered by chemical exchange of the sp(2) oxygen atom with water in industrial or laboratory procedures. Although collaborative studies are still necessary to improve the interlaboratory reproducibility of the delta(18)O parameters, consistent results can be obtained in an intralaboratory context. It is shown in particular that chemical oxidation of ferulic acid is characterized by a relative enrichment of the aromatic moiety of vanillin. 相似文献
63.
Kim YM Park K Joo GJ Jeong EM Kim JE Rhee IK 《Journal of agricultural and food chemistry》2004,52(13):4192-4196
A gene responsible for the chlorothalonil biotransformation was cloned from the chromosomal DNA of Ochrobactrum anthropi SH35B, capable of efficiently dissipating the chlorothalonil. The gene encoding glutathione S-transferase (GST) of O. anthropi SH35B was expressed in Escherichia coli, and the GST was subsequently purified by affinity chromatography. The fungicide chlorothalonil was rapidly transformed by the GST in the presence of glutathione. LC-MS analysis supported the formation of mono-, di-, and triglutathione conjugates of chlorothalonil by the GST. The monoglutathione conjugate was observed as an intermediate in the enzymatic reaction. The triglutathione conjugate has not been previously reported and seems to be the final metabolite in the biotransformation of chlorothalonil. The glutathione-dependent biotransformation of chlorothalonil catalyzed by the bacterial GST is reported. 相似文献
64.
Allen GJ Chu SP Schumacher K Shimazaki CT Vafeados D Kemper A Hawke SD Tallman G Tsien RY Harper JF Chory J Schroeder JI 《Science (New York, N.Y.)》2000,289(5488):2338-2342
Cytosolic calcium oscillations control signaling in animal cells, whereas in plants their importance remains largely unknown. In wild-type Arabidopsis guard cells abscisic acid, oxidative stress, cold, and external calcium elicited cytosolic calcium oscillations of differing amplitudes and frequencies and induced stomatal closure. In guard cells of the V-ATPase mutant det3, external calcium and oxidative stress elicited prolonged calcium increases, which did not oscillate, and stomatal closure was abolished. Conversely, cold and abscisic acid elicited calcium oscillations in det3, and stomatal closure occurred normally. Moreover, in det3 guard cells, experimentally imposing external calcium-induced oscillations rescued stomatal closure. These data provide genetic evidence that stimulus-specific calcium oscillations are necessary for stomatal closure. 相似文献
65.
Discovery of intense gamma-ray flashes of atmospheric origin 总被引:1,自引:0,他引:1
Fishman GJ Bhat PN Mallozzi R Horack JM Koshut T Kouveliotou C Pendleton GN Meegan CA Wilson RB Paciesas WS Goodman SJ Christian HJ 《Science (New York, N.Y.)》1994,264(5163):1313-1316
Detectors aboard the Compton Gamma Ray Observatory have observed an unexplained terrestrial phenomenon: brief, intense flashes of gamma rays. These flashes must originate in the atmosphere at altitudes above at least 30 kilometers in order to escape atmospheric absorption and reach the orbiting detectors. At least a dozen such events have been detected over the past 2 years. The photon spectra from the events are very hard (peaking in the high-energy portion of the spectrum) and are consistent with bremsstrahlung emission from energetic (million-electron volt) electrons. The most likely origin of these high-energy electrons, although speculative at this time, is a rare type of high-altitude electrical discharge above thunderstorm regions. 相似文献
66.
Silva JM Marran K Parker JS Silva J Golding M Schlabach MR Elledge SJ Hannon GJ Chang K 《Science (New York, N.Y.)》2008,319(5863):617-620
By virtue of their accumulated genetic alterations, tumor cells may acquire vulnerabilities that create opportunities for therapeutic intervention. We have devised a massively parallel strategy for screening short hairpin RNA (shRNA) collections for stable loss-of-function phenotypes. We assayed from 6000 to 20,000 shRNAs simultaneously to identify genes important for the proliferation and survival of five cell lines derived from human mammary tissue. Lethal shRNAs common to these cell lines targeted many known cell-cycle regulatory networks. Cell line-specific sensitivities to suppression of protein complexes and biological pathways also emerged, and these could be validated by RNA interference (RNAi) and pharmacologically. These studies establish a practical platform for genome-scale screening of complex phenotypes in mammalian cells and demonstrate that RNAi can be used to expose genotype-specific sensitivities. 相似文献
67.
68.
Dickerson RR Huffman GJ Luke WT Nunnermacker LJ Pickering KE Leslie AC Lindsey CG Slinn WG Kelly TJ Daum PH Delany AC Greenberg JP Zimmerman PR Boatman JF Ray JD Stedman DH 《Science (New York, N.Y.)》1987,235(4787):460-465
Acid deposition and photochemical smog are urban air pollution problems, and they remain localized as long as the sulfur, nitrogen, and hydrocarbon pollutants are confined to the lower troposphere (below about 1-kilometer altitude) where they are short-lived. If, however, the contaminants are rapidly transported to the upper troposphere, then their atmospheric residence times grow and their range of influence expands dramatically. Although this vertical transport ameliorates some of the effects of acid rain by diluting atmospheric acids, it exacerbates global tropospheric ozone production by redistributing the necessary nitrogen catalysts. Results of recent computer simulations suggest that thunderstorms are one means of rapid vertical transport. To test this hypothesis, several research aircraft near a midwestern thunderstrom measured carbon monoxide, hydrocarbons, ozone, and reactive nitrogen compounds. Their concentrations were much greater in the outflow region of the storm, up to 11 kilometers in altitude, than in surrounding air. Trace gas measurements can thus be used to track the motion of air in and around a cloud. Thunderstorms may transform local air pollution problems into regional or global atmospheric chemistry problems. 相似文献
69.
In experiments in which nest boxes were switched, colony foundresses of the social wasp Polistes fuscatus accepted sisters' combs with little brood destruction but destroyed younger brood in the combs of less closely related females and sometimes deserted these combs. Discrimination between related and unrelated brood does not appear to depend on prior brood contact or environmentally acquired cues. 相似文献
70.