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891.
西藏部分地区藏猪戊型肝炎病毒血清流行病学调查   总被引:1,自引:0,他引:1  
为了解西藏地区藏猪戊型肝炎流行情况,在拉萨、林芝、昌都、日喀则和山南等5个市,采集305份藏猪血清,利用酶联免疫吸附(ELISA)法进行戊型肝炎病毒血清抗体检测,并用SPSS 21.0方法进行不同性别、不同区域间的显著性差异分析。结果显示:从305份藏猪血清中检测到81份抗体阳性,总抗体阳性率为26.6%(81/305);拉萨市和林芝市的抗体阳性率最高,达32.8%(20/61),其次为昌都市、日喀则市和山南市,阳性率分别为29.5%(18/61)、26.2%(16/61)、11.5%(7/61);山南市阳性率与其他4个市相比差异显著(P0.05),公猪和母猪的抗体阳性率分别为23.3%(35/150)和29.7%(46/155),无显著差异(P0.05)。结果表明,西藏地区藏猪群中存在不同程度的猪戊型肝炎病毒感染。鉴于当地存在食用生或半生猪肉和猪肝的习俗,建议加强宣传,倡导健康饮食,同时加强高发区疫病防治,降低人类感染风险。  相似文献   
892.
Translocations of the short arm of rye (Secale cereale L.) chromosome 1 (1RS) in wheat (Triticum aestivum L. cv. Pavon 76) are known to increase root biomass. Such an increase enhances water and nutrient uptake and may improve grain yield. Two greenhouse experiments and a field experiment were carried out at the University of California, Riverside, in 2012 and 2013 under well‐watered and terminal drought treatments to evaluate phenotypic characters associated with varying dosages of 1RS, including grain yield. The genotypes used were cultivar Pavon 76 (R0), Pavon 76/Pavon1RS.1AL (F1 hybrid) with a single dosage of 1RS (R1A), Pavon 1RS.1AL with two dosages of 1RS (R2A), Pavon 1RS.1DL (R2D) also with two dosages of 1RS and Pavon 1RS.1AL‐1RS.1DL (R4AD) with four dosages of 1RS. There was a significant positive correlation between number of dosages of 1RS and root biomass. However, no correlation was found between root biomass and grain yield per plant. Drought in the field experiment reduced grain yield significantly. Under well‐watered field conditions, grain yield of R2A (215.9 g plant?1) was significantly greater than those of R2D (191.8 g plant?1) and R4AD (161.7 g plant?1). Also, grain yield of R4AD was significantly less than those of F1, Pavon 76 and R2D under well‐watered conditions. Under drought field conditions, no significant differences were found among the genotypes for grain yield was found between F1 (14.7 g plant?1) and R4AD (12.4 g plant?1). Harvest index was significantly greater in well‐watered (44.2 %) than in drought (34.6 %) field conditions. On average, genotypes F1 (42.3 %) and R2A (40.6 %) had higher harvest index than R2D (38.3 %) and R4AD (35.5 %) in the field. Also, Pavon 76 (40.2) and R2D (38.3) had higher harvest index than R4AD. Drought tolerance was lowest for R4AD due to its relatively lower grain yield potential. In general, Pavon 1RS.1AL carrying two dosages of 1RS showed higher grain yield under wet treatments. Pavon 1RS.1AL‐1RS.1DL carrying four dosages of 1RS produced the largest shoot and root biomasses, but the least grain yield.  相似文献   
893.
894.
895.
试验旨在探讨解耦联蛋白1(UCP1)基因核苷酸变异对绵羊生长性状的性别差异和胴体性状的影响,以期能够筛选出可以提升绵羊生长及胴体性状的核苷酸变异,为提高绵羊相关重要经济性状的分子遗传标记提供材料。以9个绵羊品种为研究对象,用PCR-SSCP方法检测不同性别绵羊中UCP1基因内含子5区和外显子6区变异。利用Minitab 16.0软件中一般线性模型分析内含子5区等位基因与不同性别绵羊生长性状、公羔胴体性状的关联性。结果显示,绵羊UCP1基因内含子5区和外显子6区共检测到8个核苷酸变异,其中位点c.910 G/A突变导致p.Ala304Thr氨基酸变异。生长性状关联分析结果表明,内含子5区等位基因对绵羊生长性状的影响存在性别特异性,母羔中携带等位基因A1的群体较缺失群体具有较低的初生重(P<0.05),公羔中携带等位基因C1的群体较缺失群体具有较高的断尾重(P<0.05),未发现其他等位基因与羔羊的生长性状存在性别特异性。胴体性状关联分析结果表明,携带等位基因A1的群体具有较低后腿瘦肉量、腰部瘦肉量和较高的肩部瘦肉比例(P<0.05),携带等位基因C1的群体具有较低的后腿瘦肉比例(P<0.05),其他胴体性状均没有发现与等位基因存在显著相关,基因型分析结果与等位基因分析结果一致。结果提示,UCP1基因对绵羊的生长性状影响具有性别特异性,且携带等位基因A1的公羔群体具有较低的胴体生产性状,为提高公羔胴体生产性能提供了理论依据。  相似文献   
896.
Adsorbed phosphate in soils can be chemically extracted; however, this process is both time‐consuming and not cost‐effective if large numbers of samples have to be analysed. Indirect assessment of adsorbed phosphate by pedotransfer functions (PTFs) can help optimize fertilizer strategies. This study aimed to evaluate the spatial variability of adsorbed phosphate (Pads), iron oxides and magnetic susceptibility (MS) in oxisols and to calibrate PTFs to predict Pads. A total of 308 soil samples were collected from Hapludox and Eutrudox soils formed from sandstone in Brazil. The contents of clay (196–607 g/kg), iron oxides (40–165 g/kg), MS (1.2–29 × 10?6 m3/kg) and Pads (327–842 mg/kg) were in the range of typical values for these highly weathered soils. This study showed that the attributes studied were spatially dependent. Geomorphic surfaces enabled understanding of spatial variability and helped to develop a more efficient sampling scheme to calibrate PTFs. Moreover, the adsorbed phosphate in these oxisols could be predicted by a PTF using iron oxides and MS as predictors. The MS attribute enabled the most accurate prediction (concordance coefficient = 0.95, root‐mean‐square error = 46 mg/kg and relative improvement in root‐mean‐square error = ?4.12) of spatial variability through PTF compared to other predictors.  相似文献   
897.
We studied the black carbon (BC) content of ca. 405 samples from French topsoil and artificial soil and carbonate mixtures. Our protocol involved three main steps: (i) decarbonation by HCl, (ii) elimination of non‐pyrogenic organic carbon in a furnace at 375 °C, and (iii) quantification of residual carbon by CHN analysis. BC content increased for calcareous soils according to their carbonates content. Subsequent analyses confirmed the existence of a methodological artefact for BC determination only in calcareous soils. Decarbonation changes the thermal properties of organic matter, creating more recalcitrant carbon than in the initial sample. Higher CaCO3 and organic carbon content results in a more pronounced artefact. The reversal of the first two steps of the chemo‐thermal oxidation method (i.e. thermal oxidation before soil decarbonation) eliminates this artefact. Overall, our results suggest that BC content may have been overestimated in a large number of studies on calcareous soils.  相似文献   
898.
A potentially significant cause of damage to grassland soils is compaction of unsaturated soil and poaching of saturated or nearly saturated soil by animal hooves. Damage is caused when an applied stress is in excess of the bearing strength of the soil and results in a loss of soil structure, macroporosity and air or water conductivity. Severely damaged soils can cause reduced grassland productivity and make grazing management very difficult for the farmer. The actual amount of soil damage that can occur during grazing is dependent on the grass cover which acts as a protecting layer, the soil water content and the characteristics of the grazing animal (weight and hoof size). Assuming that the farmer is knowledgeable about the characteristics of the grazing animal and grass cover, it would be very useful for short‐term operational farm planning to be able to predict when soil water contents were likely to be in a critical range with respect to potential hoof damage. In this study soil moisture deficits (SMDs) which can be derived from meteorological forecasts are evaluated for predicting when soil water conditions are likely to lead to hoof damage. Two contrasting Irish grassland soils were analysed using a Hounsfield servo‐mechanical vertical testing machine to simulate static (285.4 N) and dynamic (571 N) hoof loads on the soil over a range of estimated SMDs (0, 5, 10 and 20 mm). The deficits were analysed with respect to the soil volumetric water content, compression (displacement) and change in dry bulk density. The SMDs imposed in the laboratory were similar to those under field conditions and thus the methods used in this study are applicable elsewhere. The change in dry bulk density following loading (0.2–0.7 g/cm3) was linearly related to SMD (R2 ranged from 0.90 to 0.99), leading to the conclusion that a forecast of SMD can be used to predict when grassland soils are likely to be at risk of damage from grazing.  相似文献   
899.
The effects of various copper (Cu) concentrations on the antioxidative system in the roots of Medicago sativa were explored. The results indicated that the Cu content of the roots reached a value of 854 μg g?1 DW at 10 μm Cu and a value of 4415 μg g?1 DW at 100 μm Cu, suggesting that M. sativa has better ability to tolerate and accumulate Cu than other Cu‐bioaccumulators, and is a potential plant for phytoremediation. Treatment with Cu resulted in a significant increment in the levels of H2O2, O2˙? and OH˙. The reduced form of ascorbate and glutathione reached a peak at 30 μm Cu, and was followed by a sharp depletion to a lower level than that of the control. In contrast, the levels of the oxidised forms of ascorbate and glutathione showed a progressive increment with increasing Cu concentrations, suggesting that the antioxidant system was unable to cope with Cu stress at higher Cu levels. Under the Cu concentrations tested, the activity of catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC 1.6.4.2) increased at lower Cu concentrations, and then decreased, reaching a maximum at 30 μm of Cu for APX and GR, at 10 μm for CAT, whereas the activities of guaiacol peroxidase (POD, EC 1.11.1.7) were gradually increased with increasing Cu concentrations. PAGE analysis of superoxide dismutase (SOD, EC 1.1.5.1.1) revealed that one band is a Mn‐SOD and five bands are identified as Cu, Zn‐SOD, whereas Fe‐SOD isoforms were not found in the roots of alfalfa. Cu at 10–100 μm increased the intensity of constitutive isozymes of CAT, APX and POD, whereas it decreased the intensity of isozymes of glucose‐6‐phosphate dehydrogenase (G6PDH, EC 1.1.1.49) significantly. The activities of lipoxygenases (LOX, EC 1.13.11.12) were gradually augmented with increasing Cu concentrations, demonstrating that LOXs are probably involved in production of lipid hydroperoxides and superoxide anion. There was a continuous and pronounced enhancement in the activity of esterase (EST, EC 3.1.1.1) in roots treated with 10–30 Cu μm , whereas EST activity in roots exposed to above 30 μm Cu declined, suggesting that EST plays a protective role under lower Cu concentrations stress.  相似文献   
900.
The effect of 0, 0.05 or 0.1 mm abscisic acid treatment on chilling tolerance and salicylic acid‐related responses was investigated in young maize seedlings (Zea mays L., hybrid Norma). Although the pre‐treatment of maize seedlings with abscisic acid slightly decreased the chlorophyll content, it also reduced the level of chilling injury caused by 6 days of cold treatment at 5 °C. Under normal growth conditions, increased levels of bound salicylic acid and of bound ortho‐hydroxycinnamic acid were observed in the leaves during abscisic acid treatment. In the roots, abscisic acid did not affect the free and bound salicylic acid levels, but increased the amount of free and bound ortho‐hydroxycinnamic acid. The activity of glutathione‐S‐transferase increased on the 3rd day of abscisic acid treatment, whereas it did not change when followed by cold stress, compared with the control leaves. In the roots, the activities of glutathione reductase, glutathione‐S‐transferase and ascorbate peroxidase increased during the abscisic acid treatment, and those of glutathione‐S‐transferase and ascorbate peroxidase were also stimulated when abscisic acid pre‐treatment was followed by cold stress, compared with the control roots. Our results suggest that an overlap may exist between the abscisic acid‐induced cold acclimation and the salicylic acid‐related stress response.  相似文献   
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