Response of carnation cultivars to Fusarium oxysporum f.sp. dianthi in the field

The response of carnation (Dianthus caryophyllus L.) cultivars toFusarium oxysporum f.sp.dianthi (F.o.d.) was evaluated in an artificially infested field from 1988/9 to 1991/2. Disease incidence was highly correlated with disease severity, indicating that disease incidence may be used to estimate the impact ofF.o.d. on the host. Based on the results, the following stepwise procedure was developed for characterizing the response of carnation cultivars toF.o.d. First, the general response of the tested cultivar was classified as resistant or susceptible on the basis of disease incidence values recorded 180 days after planting. Empirical analysis of the data revealed that a disease incidence level of 75% may be taken as a reliable cut-off point for separation of cultivars into the two groups. Within each group, cultivars were then subjected to a more explicit classification: in the resistant group the records of actual disease incidence were used for classification, while in the susceptible group the linear rearrangement of the disease progress curve was calculated according to the Gompertz function, and the value of the intercept was used for classification.Contribution No. 3539-E series, from the Agricultural Research Organization.     

A New Approach for Minimizing Ammonia Emissions from Poultry Houses

The paper describes a novel approach to reduce ammonia emissions from Concentrated Animal and Feeding Operations (CAFO) in general, and from poultry houses in particular. The approach is based on installing a dedicated air capturing system on the feeding infrastructure that draws air from close to the litter. Air at these locations has NH_3(g) concentrations an order of magnitude higher than at the vents of the ventilation system. Moreover, while the dedicated waste air drawing system can work continuously, the operation of the ventilation system is intermittent and directed towards maintaining the birds climatically-comfort. The NH_3(g) rich waste air is conveyed to an acidic (0?<?pH?<?~5) bubble column reactor in which ammonia is converted to ${\text{NH}}_4^ + $ . The reactor operates in a batch mode, starting at pH?0 (1?N HCl solution) and is switched to a new acidic absorption solution just before NH_3(g) breakthrough occurs, at around pH 5. Experiments with a wide range of NH_3(g) concentrations showed that the absorption efficiency is practically 100% throughout the process as long as the face velocity is below 4 cm/s. The advantages of the method include high absorption efficiency, lower NH_3(g) concentrations in the vicinity of the birds, generation of a valuable product (a high concentration ammonia solution) and the separation between the ventilation and ammonia treatment systems. A small scale pilot operation conducted for 5 weeks in a broiler house showed the approach to be technically feasible. A larger scale pilot study is required for fine-tuned cost estimation.     

Nutrient-specific foraging in invertebrate predators

Many herbivores and omnivores adjust their food selection behavior to regulate the intake of multiple nutrients. Carnivores, however, are generally assumed to optimize the rate of prey capture rather than select prey according to nutrient composition. We showed experimentally that invertebrate predators can forage selectively for protein and lipids to redress specific nutritional imbalances. This selection can take place at different stages of prey handling: The predator may select among foods of different nutritional composition, eat more of a prey if it is rich in nutrients that the predator is deficient in, or extract specific nutrients from a single prey item.     

Response of Quercus pyrenaica (melojo oak) to soil water deficit: a case study in Spain

In the present study, carried out from 2004 to 2006, leaf and stem water potential and stem water content were measured in Quercus pyrenaica Willd. individuals in an experimental forested catchment located in Central Western Spain under Mediterranean subhumid conditions. These indicators of tree water status were compared with soil moisture contents measured in the same area from 0 to 1 m depth and from 0 to 2.5 m depth during the last year of the study. The objectives were to clarify the seasonal and year-to-year variations in tree water status, to examine applicability of stem water content as useful water stress indicator and to discuss how deep soil water and root uptake contribute to survival during the long dry summer. Seasonal variations in the tree variables measured revealed a typical pattern, with maximum values at the end of spring followed by a progressive decline during the summer drought in response to the decrease in soil water content (almost exhausted at 0–100 cm depth). The relatively high values and the non-significant variation in predawn leaf water potentials (except for 2005, which was exceptionally dry) indicate that no clear water stress situations occurred. This may be explained in terms of a progressive absorption of water from the deeper layers. The results also suggest that the stem water content is a more sensitive indicator of long-term water limitation than the other variables measured.     

Controlling the growth and development of rose plants after planting

The effects of pinching and lateral bud removal (deshooting) on the development of structural shoots, the number of flowers, and the average flower stem length in young rose plants were examined, using the cultivars ‘Baccara’, ‘Sonia’ and ‘Belinda’. The effect of deshooting on flower yield varied with each cultivar. It did not affect the number of ‘Baccara’ flowers, but increased ‘Sonia’ and ‘Belinda’ flower production by 50% and 75%, respectively. Leaving flowers to bloom out on plants before the start of commercial harvest resulted in a decrease in the length of the flower stems and also reduced the beneficial effect of deshooting on flower yield. Deshooting enhanced structural shoot formation (“bottom breaks”). Pinching flower buds of structural shoots increased the number of ‘Baccara’ flowers in comparison with pruning these shoots to 40–60 cm, as in common practice. Deshooting of the structural shoots of ‘Sonia’ and ‘Belinda’ increased the number of flowers in both cultivars.     

Neospora caninum is a cause of perinatal mortality in axis deer (Axis axis)

Neospora caninum is a worldwide distributed protozoan that may cause neuromuscular disease in dogs and reproductive failure in domestic and wild ruminants. One axis fawn (Axis axis) and four neonates from the same deer herd died at a zoo in Argentina within a four-month period. The fawn presented with dilatation of the anal sphincter at birth and incontinence, developed weakness and ataxia and died at 14 days of age. At necropsy, a mega formation of the distal large intestine was observed. Microscopically, non-suppurative encephalitis, suppurative bronchopneumonia, fibrin necrotic enteritis and degenerative changes in the liver were observed in hematoxilin and eosin-stained tissue sections, and thick-walled N. caninum-like cysts were observed in fresh brain samples. Serologic studies for N. caninum revealed an IFAT titer of 1:6400 in the fawn and 1:25, 1:400, 1:3200 and 1:6400 in the neonates. N. caninum DNA was detected in brain samples from the fawn and from one neonate by PCR, and the parasite was isolated in vitro from the fawn’ brain after passage through gerbils (Meriones unguiculatus) and gamma-interferon knock-out mice. N. caninum DNA obtained from the fawn, neonate and isolated parasites showed the same microsatellite pattern. This suggests a common infection source for both animals.The diagnosis of N. caninum infection was confirmed, suggesting its association with perinatal mortality in captive axis deer. To the best of our knowledge, this is the first report of clinical disease associated to N. caninum infection in axis deer and of isolation of the parasite from this wild ruminant species.     

Computed tomography is a feasible method for quantifying bone density in Macaca mulatta

Osteopathologies are a result of advanced age and decreased bone density and represent a global health problem. It is therefore important to generate models for longitudinal studies of the pathophysiology in order to improve early diagnosis and develop preventive therapies. For this kind of research, the use of computed tomography (CT) to evaluate bone health offers advantages over other techniques since it provides more complete information. The aim of this prospective, pilot study was to obtain measurements of the left femur from a population in captivity of 32 rhesus monkeys (Macaca mulatta) in order to standardize the model for future research. Healthy subjects from 5 to 28 years old were chosen. Three groups with different ages were formed as follows: (1) 5–9 years, (2) 10–19 years, and (3) 20–28 years. Semi‐automatic segmentation by threshold defined the regions of interest, which were subdivided in the range of 300–700 Hounsfield units (HU) for trabecular bone and >700 HU for cortical bone. Then, the proportional ratios of the volumes of trabecular bone and cortical bone were obtained. Significant differences (analysis of variance test) in the averages of Hounsfield units, cortical, and trabecular bone proportions from each age group proved that a decrease in bone density begins at approximately 20 years of age. The values presented here, as well as the method to obtain them from CT scans, can be used as a baseline in a primate model for long‐term research in bone pathology diagnosis and treatment.