Isolation and identification of a lipase producing Bacillus sp. from soil

Lipase production in an indigenous lipolytic Bacillus sp. was detected in media containing Tributyrin-Tween 80 and Rhodamine B-Olive oil. The statistical Taguchi model was used to predict the optimum experimental conditions for bacterial growth and lipase production. Partial optimization was carried out for selection of salt base, oil, glucose, NH4Cl and yeast extract concentrations, inoculum density, pH and agitation. Maximum lipase activity was detected in the cell free supernatants of cultures grown in a medium containing 10 g L(-1) yeast extract, 15 g L(-1) NH4Cl, 3 g L(-1) K2HPO4, 1 g L(-1) KH2PO4, 0.1 g L(-1) MgSO4 x 7H2O, 2 g L(-1) glucose, 0.6 mM MgCl2 and 15 ml L(-1) olive oil, pH 8.5 at 30 degrees C for 24 h and low agitation. The amount oflipase produced in the designed medium was in agreement with the predicted values by the statistical method. 16S rRNA cloning and sequencing identified the test organism as Bacillus pumilus.     

Estimation of the organic carbon content by the pattern recognition method

Studying the status of agricultural soils is one of the most important concerns in the agricultural sector. The soil organic carbon (SOC) is one of the main parameters and it plays an important role in improving soil properties. Hence, knowing this parameter is important in soil science. This study applied the pattern recognition (PR) method in predicting the SOC. Also, the ability of this method was compared with different methods such as the Radial Basis Function Network (RBF), Multilayer Perceptron Neural Network (MLP), Multiple Linear Regression (MLR) and Support Vector Regression (SVR). To compare the results, four performance criteria, namely, root mean square errors (RMSE), the Nash–Sutcliffe efficiency (NS), Willmott’s Index of agreement (WI), mean absolute error (MAE) and Taylor diagrams were used. Results indicated that the PR model performed significantly better than the MLP, MLR, SVR, and RBF models for the estimation of the SOC.     

Effects of melatonin on oocyte maturation in PCOS mouse model

The purpose of oocyte in vitro maturation is generation of mature oocytes that could support future development. Efforts have been made to enhance oocyte developmental competence by developing optimal culture conditions. The present study is conducted to determine melatonin effects on quality of polycystic ovarian syndrome (PCOS) oocytes when it has been added during in vitro maturation, and immature oocytes were cultured in defined conditioned medium with and without different melatonin concentrations. Melatonin could significantly improve nuclear maturation of PCOS oocytes (81.1% vs. 56.3%, P < 0.05 were achieved with 10^?6 mol/L concentration). Cleavage rate was significantly higher in 10^?5 mol/L concentration compared to untreated oocytes in PCOS (54% vs. 35%, respectively) and it was significantly higher with 10^?6 mol/L concentration in the control group, 55% versus 38%, compared to untreated oocytes. This study showed that melatonin has the potential to induce oocyte nuclear maturation and guarantee fertilization potential. © 2016 Japanese Society of Animal Science     

Effects of neonatal diarrhea and other conditions on subsequent productive and reproductive performance of heifer calves

Diarrhea is one of the most frequent diseases of neonatal calves in dairy herds. The aim of this study was to evaluate the effects of neonatal diarrhea and other conditions on subsequent first lactation milk production and reproductive performance of heifer calves up to the first calving. Seven hundred heifer calves (350 with and 350 without the history of diarrhea in first month of life) were monitored from birth until a year after calving. For each heifer, birth season, birth weight, ease of birth and occurrence of diseases from birth to the end of first lactation were recorded as independent variables. Interval from birth to first service, interval from birth to conception, interval from birth to first calving and 305-days milk yield in the first lactation were recorded as dependent variables. Effects of explanatory variables on productive and reproductive indices were evaluated by survival analysis and general linear model. Heifers with the history of diarrhea during the first month of their lives showed lower daily hazard of conception [Hazard ratio: 0.85 (95%CI: 0.73–0.99)] and calving [Hazard ratio: 0.84 (95%CI: 0.72–0.98)] than those of healthy heifers. Subsequent reproductive performance of heifer calves was also associated with birth season and ease of birth. Further, heifer calves born with birth weight of below 35 kg produced an average of 518 and 506 l less milk in their first lactation compared to calves with a birth weight 40–45 (p = 0.013) and greater than 45 kg (P = 0.033), respectively. These findings showed that occurrence of neonatal diarrhea and other neonatal parameters are associated with harmful effects on subsequent production and reproductive performance.     

Projected impacts of global warming on cropping calendar and water requirement of maize in a humid climate

In this research, the influence of climate change on maize cultivation was investigated and then, the possible solutions for adopting this natural hazard in the coasts of Caspian Sea in Iran was assessed. Weather data were generated for the 2011–2100 period using a statistical downscaling model under different climatic scenarios. Reference evapotranspiration (ETo) was calculated using a Neuro-fuzzy inference system. Cop water requirement was calculated by multiplying ETo by crop coefficients. Increased cardinal temperatures during 2011–2100 led to shifting in the planting date backward by 10–26 days. In addition, the projected global warming has a considerable effect on the duration of the vegetative growth stage resulting in earlier harvesting. However, the duration of the reproductive stage is less affected. Despite the obvious reduction in the length of the growing season, crop water requirement will increase by 10.6–15.3% in the future due to 1.64–28.4% increase in ETo. However, changing the cultivation time may lead to 11.2–264.5 m³ ha^?1 water saving during the whole cropping cycle through affecting both ETo and the crop growth cycle. This result demonstrates that management of the maize cropping calendar can be an effective way to achieve sustainable agriculture under future climate conditions in the study area.     

Mechanical properties and in vitro degradation of PLGA suture manufactured via electrospinning

The current research discusses the efforts to achieve a Poly(lactide-co-glycolide)(PLGA) nanofiber yarn using two differently charged nozzles with potential application as surgery suture. First, electrospinning parameters such as solution concentration, applied voltage, feed rate were optimized to produce yarn with smooth nanofibers. In order to improve the properties of produced suture, heat setting setup was developed. Two heat setting techniques, including hot water and dry heat were applied, and the influence of the heat setting process on the mechanical properties of yarn was studied. The results showed that heat setting with boiling water was the best method. At first strength, E-modulus and extension of prepared suture were 36.6 MPa, 0.9 GPa and 68.8 % respectively. After improvement with heat setting, strength and E-modulus increased to 63.7 MPa, 2.7 GPa respectively and extension decreased to 29.7 %. Finally, in order to analyze knot performance, two types of surgical knot (square and surgeon) were used, and mechanical properties were investigated. The presence of knot lessens mechanical properties for each two type. Square knot showed better mechanical properties than surgeon’s knot. With square knot strength, E-modulus and extension were 62.1 MPa, 2.1 GPa, 28.6 %, respectively. In vitro study of nanofiber yarn degradation behavior showed that the mechanical properties were decreased. This could be due to greater surface area of nanofibers exposed to surrounding environment.     

Effects of Different Types of Composts on Soil Characteristics and Morphological Traits of Two Dry Rangeland Species

Today, environmental protection and safe crop production are very important. The management of soil elements by compost is considered as important for sustainable agriculture. The mode of action of the composts is very different between various plant species. To evaluate the effects of different composts on soil structural and chemical properties and on morphological traits of two dry rangeland species (atriplex; Atriplex lentiformis and mesquite; Prosopos juliflora), a study was conducted in Fars Province of Iran during the year 2010. The study was a factorial experiment based on a randomized complete block design with three replications. Treatments included compost types: solid (SC) and liquid compost or compost tea (LC), solid-liquid mixture (XSL) and control (Con; non used compost) as the first factor, and two pasture plant species as the second factor. The results showed that the compost application had significant and positive effects on morphological traits such as plant height, stem diameter, plant volume, crown length, width, and area, and caused 15, 51.18, 70.67, 34.18, 18.35, and 64.94% increase on these morphological traits, respectively. Although soil acidity was not significantly affected by compost and species, the effects of compost were significant on organic matter percentage, soil phosphorous, and potassium contents. Soil nitrogen percentage was affected by both species and compost. Compost application caused a decrease in the amount of sodium compared with the control. Overall, the results of this study suggested that within the compost types, liquid compost was an advisable biofertilizer in a similar climate. Furthermore, the LC and the XSL are recommended for improving the morphological traits and the soil characteristics, respectively.     

Effect of dietary Spirulina platensis on fatty acid composition of rainbow trout (Oncorhynchus mykiss) fillet

This paper reports on inclusion of Spirulina platensis (SP) in rainbow trout (Oncorhynchus mykiss) fish diet and its effect on the fish tissue fatty acids (FAs) composition. Fish in triplicate groups with average initial body weight of 75 ± 0.23 g and age of about 7 months were fed a control diet with no supplements and with three experimental diets supplemented with 2.5, 5 and 7.5 weight percent (wt%) of SP for 12 weeks. At the end of experiment, the fillet FA compositions were evaluated. It was found that the composition of FAs in fish fillet was significantly (P < 0.05) affected by inclusion of SP in diets. As compared to the control sample, feeding the fish supplemented particularly with low levels of SP improved the quality of FAs by decreasing undesirable saturated FAs (SFAs) content from 20.03 to 17.93 % and increasing the level of some beneficial long-chain highly unsaturated FAs namely eicosapentaenoic acid (C20:5 n-3) from 6.47 to 7.27 %, docosapentaenoic acid (C22:5 n-3) from 1.42 to 1.56 % and docosahexaenoic acid (C22:6 n-3) from 1.26 to 1.35 %. Further increase in the SP inclusion level generally did not lead to desirable results. The SP inclusion in the fish diet had no significant impact on overall contents of n-3 and n-6 polyunsaturated FAs in fish fillet. However, the total highly unsaturated FAs (HUFAs) and n-3 HUFAs levels, UFAs/SFAs ratio and n-3/n-6 index were increased from 11.25, 10.65, 3.98 and 0.57 to 12.13, 11.56, 4.40 and 0.62 %, respectively, as a result of 5 wt% SP loading. In general, it can be concluded that SP inclusion up to 5 wt% of loading in rainbow trout culture can be beneficial in terms of FAs compositions of the fish fillet.