30℃条件下玉米自呼吸对储藏环境中气体浓度变化的影响研究

通过对3种不同水分(偏低水分11.8％、安全水分13.3％和偏高水分16.3％)的玉米在30℃条件下,密闭储藏环境内N2、O2和CO2百分浓度变化的测定,研究密闭储藏环境条件下玉米粮粒周围环境气体成分浓度的变化规律.20 L规模的试验室研究结果表明:不同水分玉米在密闭储藏时环境中N2浓度随时间的变化均不大;不同水分玉米在密闭储藏时环境中O2浓度与储藏时间呈负相关;不同水分玉米在密闭储藏时环境中CO2浓度与储藏时间呈正相关.在气调储藏时,对偏低水分和安全水分的玉米可充分利用粮粒的自呼吸使环境中的O2浓度在21 d内迅速下降,后期可通过粮粒的自呼吸使O2浓度维持在5％左右,偏高水分的玉米在11 d内即可使O2浓度迅速下降,但后期O2浓度接近0％,无氧呼吸加剧.通过对玉米密闭储藏环境中气体浓度以及耗氧量和CO2累积量随时间的变化趋势线进行回归分析,得到30℃条件下不同水分玉米密闭环境中N2、O2和CO2的回归方程以及耗氧量和CO2累积量的回归方程,利用相应回归方程,可获得玉米在密闭储藏时环境中不同储藏时间段的气体浓度,为气调储藏时玉米粮粒自呼吸的合理利用提供基础技术参数和数据模型.     

Phenotypic and genotypic analysis of a commercial cultivar and wild populations of Anemone coronaria

Seven wild populations of Anemonecoronaria were assessed for 11 phenotypic traits, most of them having economic value for the flower industry. The wild populations were sampled to represent the diversity in habitats, climates, rock and soil types, terrains, and elevations in Israel. AFLP analysis was carried out on 12 individuals from each of six out of the seven wild populations and for six individuals from the commercial cultivar ‘Mona-Lisa’. It was found that the Dorot population, which is located in the area bordering the semi-arid zone at the very end of the species distribution, exhibits extreme and different phenotypes with relatively low variability compared withthe other wild populations. The other six wild populations, that grow in more favorable geographic and climatic conditions exhibit phenotypes with larger plants, larger numbers of flowers and less dissected leaves. These populations were less uniform than that of Dorot. Genetic characterization by AFLP markers revealed a total of 165 bands. The wild populations exhibit wide variation within-population, with about 80% polymorphic bands and average gene diversity between pairs of about 30%. The Dorot population has the lowest genetic variation and the Megido population the highest. Thus, the phenotypic variation reflects the genetic variation. The cultivar ‘Mona-Lisa’, as expected, has much lower genetic variation. The Dorot population and the ‘Mona-Lisa’ cultivar were found to have the largest genetic distances from the other wild populations, and the highest genetic variation between themselves. Phenetic analysis yielded a dendrogram describing the genetic relatedness of these populations. This revised version was published online in August 2006 with corrections to the Cover Date.     

品种推广的现状问题与建议

1目前品种推广的基本情况1．1新品种数量不断增多，丰富了种子市场《种子法》实施后，植物新品种所有人的合法权益得到保护，选育的品种得到推广应用，育种者依法获得了相应的经济利益，改变了过去育种者无偿育种的局面，激发了广大育种者的积极性和创造力，农作物新品种尤其是“两杂”（杂交水稻、杂交玉米）新品种育种速度加快，     

土体固结与溶质运移三维耦合模型

考虑堆场上覆堆积体自重引起的土体固结与溶质运移的耦合效应已成为环境土工领域中的热点问题。在Biot固结理论和溶质运移理论相结合的基础上,以孔隙率作为耦合参量,将固结方程与运移方程相联系,考虑土体固结对溶质运移模型参数的影响,建立了溶质在固结变形土体中运移的三维耦合模型。采用多场耦合有限元软件COMSOL Multiphysics对模型进行了数值求解。数值模拟结果表明,土体固结变形对溶质运移过程具有较强和持久的阻滞作用,导致溶质运移深度以及污染羽范围的减小,在模拟运移20 a时,运移距离减小率已高达27.75%,且随着时间的增加而逐渐增大;溶质在水平方向的迁移距离不可忽略,随着横向水力梯度的增大而增大。     

γ射线辐照水稻成熟花粉的杂交后代突变效应分析

本研究建立了一套简便、行之有效的水稻成熟花粉辐照诱变技术的工作程序，解决了水稻成熟花粉保鲜的技术难关，提出了水稻成花粉适宜诱变剂量为46Gy左右，确认了辐照花粉杂交后代以产生数量性状突变为主，第三代较第二代出现更多的突变性状，扩大了突变幅度，而且随着世代的递增，突变性状有累加的现象，大部分株系在第五代即可稳定。利用本项技术获得了一批熟期提早或推迟15天以上，株高变矮或增高10cm以上、稻谷增长5mm，米长增加4．4mm，长宽比最大达4．5，千粒重较起始种增减10-23g等特殊性状的一批新种质。本研究初步认为水稻成熟花粉辐照诱变主要是诱发DNA位点的突变，同时存在隐性突变，在水稻及其它作物育种中具有应用前景。     

Cultivar identification and genetic map of mango (Mangifera indica)

Amplified Fragment Length Polymorphism (AFLP) information was used for identification of mango (Mangifera indica L.) cultivars, for studying the genetic relationship among 16 mango cultivars and seven mango rootstocks and for the construction of a genetic linkage map. Six AFLP primer combinations produced 204 clear bands and on the average 34 bands for each combination. The average Band-Sharing between cultivars and rootstocks was 83% and 80%, respectively. The average Band-Sharing for mango is 81%. The probability of obtaining a similar pattern for two different mango cultivars and rootstocks is 6 × 10⁻³and 2 × 10⁻³, respectively. A preliminary genetic linkage map of the mango genome was constructed, based on the progeny of a cross between ‘Keitt’ and ‘Tommy-Atkins’. This linkage map consists of 13 linkage groups and covers 161.5 cm defined by 34 AFLP markers. This revised version was published online in August 2006 with corrections to the Cover Date.     

作物水分利用率的影响因素及其提高途径探讨

综述了作物水分利用率的研究进展。在探讨水分利用率内涵的基础上，阐述了作物水分利用率的影响因素如作物，环境因素，化学制剂，栽培措施和耕作制度等人类活动等影响因素，重点分析了土壤因素和化学制剂对作物水分利用率的影响，探讨了作物水分利用率的提高途径，包括优化种植结构；选用节水高产型品种；农田覆盖保水技术；水肥耦合技术；化学调控节水技术和节水灌溉技术等     

葡萄糖氧化酶基因在棉花中的高效转化——转基因再生棉株的获得

利用农杆菌介导法把葡萄糖氧化酶基因转移到棉花中 ,获得了再生植株 ,并对影响棉花愈伤组织分化和植株再生的因素作了探讨。离体分子检测表明 ,外源基因已整合到棉花基因组中     

粤甜9号种植密度对产量及效益的影响

以超甜玉米粤甜 9号为材料 ,研究不同密度对产量及种植效益的关系。结果表明 ,适宜密度应在 4 80 0 0～ 5 4 0 0 0株 hm2 ,过高或过低都会影响鲜苞商品性状、产量及经济效益。最适宜栽培密度在 4 80 0 0株 hm2 ,此时鲜苞商品率好 ,产量和种植效益均较高。     

小麦抗病基因同源序列(RGAs)的克隆与分析

RGA(抗性基因同源序列)法是克隆植物抗性基因的一种经济有效的方法,成为近年来的研究热点。本实验综合分析了拟南芥,西红柿,水稻,烟草等植物已克隆的抗性基因,并以这些抗性基因的NBS(核酸结合位点),LRR(富含亮氨酸重复),STK(丝氨酸/苏氨酸激酶)保守结构域设计并合成了几十对RGA引物,对小麦抗条锈病材料进行PCR扩增,获得以Xal-NBS为引物的R88RGA片段,经克隆和序列比对分析,发现该片段与逆境条件下植物抗病信号传导相关,与蛋白激酶同源性达到96%。此项研究对抗病机理的研究和基因的发掘有重要的指导意义。