施用茚虫威饵剂后红火蚁的蚁巢迁移及弃尸行为

红火蚁Solenopsis invicta是典型的社会性昆虫, 在防控过程中容易引起红火蚁巢体迁移和弃尸等现象, 影响防控效果。本研究对标记好的蚁巢施用茚虫威饵剂, 然后定时观察新增加的蚁巢数量和工蚁弃尸量。结果表明, 施用0.08%和0.1%茚虫威饵剂后, 所有处理均有新蚁巢出现, 施药后5~10 d, 新蚁巢大量出现, 随后蚁群趋向稳定, 新增蚁巢减少, 至处理后25~30 d, 再次出现新蚁巢。0.1%茚虫威饵剂15 g/巢处理在施药后5 d出现8个新蚁巢, 药后10 d和30 d分别又新增5个蚁巢, 其他时间新增蚁巢数较少。按处理剂量, 药后30 d, 2种药剂15 g/巢处理出现的新巢数量均显著高于25 g/巢处理。0.1%茚虫威15 g/巢处理, 校正蚁巢累计增加率高达124.98%, 0.08%茚虫威饵剂15 g/巢处理为89.05%; 0.08%和0.1%茚虫威饵剂25 g/巢处理, 校正蚁巢累计增加率分别为12.00%和51.34%。施用15 g/巢饵剂后2 d开始有大量工蚁尸体被丢弃, 至药后4 d弃尸量达最大值, 0.08%和0.1%茚虫威饵剂处理弃尸量分别为330.5头/巢和300.2头/巢, 随后呈逐步下降趋势, 至14 d, 弃尸量降至最低。施药后8 d内弃尸量达总弃尸量75.26%以上, 至药后10 d, 已达总弃尸量的90.08%以上。施用0.08%和0.1%茚虫威饵剂25 g/巢的处理新蚁巢出现较少, 表明施用相对低剂量的饵剂, 更容易引起蚁巢迁移。红火蚁的弃尸行为主要发生在饵剂施用后的前8 d。     

贵州荞麦重要种质的遗传多样性分析及分子身份证构建

为精准鉴定贵州荞麦种质资源,采用SSR分子标记对60份荞麦种质进行遗传多样性分析,构建DNA分子身份证数据库。结果显示,从100对SSR引物中筛选出16对稳定性好、多态性丰富的引物,在60份供试种质中共扩增出174个多态性条带;Shannon’s信息指数、Nei’s多样性指数、多态信息指数均值分别为0.337、0.206、0.693,引物的多态性较好,能有效揭示60份荞麦种质的遗传多样性;在Dice遗传相似系数为0.374时,所有供试材料可聚为A、B、C 三组;当Dice遗传相似系数为0.484时,将苦荞组（A组）更细分为A1、A2两个小组。采用毛细管电泳及8%的聚丙烯酰胺凝胶（PAGE）电泳对SSR标记扩增产物进行双验证,2种方法的聚类结果一致。结果表明,本研究开发的高效性SSR分子标记能够有效地鉴定贵州荞麦重要种质的遗传多样性且用于构建分子身份证。     

野老鹳草Geranium carolinianum种子的萌发条件

为明确野老鹳草Geranium carolinianum种子的休眠特性及萌发适宜的环境条件,采用培养皿法和盆钵法研究野老鹳草种子萌发和出苗适宜的条件,记录种子萌发数,计算种子萌发率、平均萌发时间及萌发指数。结果显示,野老鹳草具有较长的休眠期,新采集的野老鹳草种子在室温干储210 d后可以解除休眠;在10~25℃的恒温条件下种子萌发率均在93.8%以上;有无光照及光周期的长短对种子的萌发无影响;对酸碱度不敏感,在pH 4~10范围内种子萌发率均在91.3%以上;对水势具有一定耐受力,抑制50%种子萌发率所需的水势为-0.42 MPa;盐分对野老鹳草种子萌发具有一定抑制作用,当盐浓度达到160 mmol/L时基本不能萌发;此外,种子在土壤垂直深度5 cm以内时仍然可以出苗,对播种深度适应性较强。表明野老鹳草种子具有较长休眠期,土壤层积可解除种子休眠;而且野老鹳草种子对温度、光照、土壤酸碱度、播种深度适应性较强,水势及盐分对种子萌发有一定影响。     

Sulodexide protects against hyperglycemia-induced retinal vascular injury via suppressing NOX4/NLRP3 pathway

AIM To investigate the effect of sulodexide (SDX) on high glucose-induced damage in retinal microvascular endothelial cells. METHODS (1) High-fat diet combined with intraperitoneal injection of streptozocin were used to induce type 2 diabetes mellitus (DM) followed by injection of saline or SDX in C57BL/6J male mice. Retinal microvascular leakage and density, and the protein levels of NLRP3 inflammasome-related proteins, zonula occludens-1 (ZO-1) and NADPH oxidase 4 (NOX4) were measured. (2) Human retinal microvascular endothelial cells (HRMECs) were treated with normal glucose or high glucose with or without SDX, and were further transfected with siRNA to knock down NOX4, or infected by adenovirus to over-express NOX4. The protein levels of ZO-1, VE-cadherin (VE-Cad), NOX4 and NLRP3 inflammasome-related proteins as well as the level of reactive oxygen species (ROS) were detected. RESULTS Treatment with SDX increased the protein level of ZO-1, attenuated retinal leakage and NLRP3 inflammasome activation, and enhanced the density of microvasculature and the number of ganglion cells in diabetic retinas. The protein levels of ZO-1 and VE-Cad were decreased, while the levels of NOX4, NLRP3 inflammasome-related proteins and ROS generation were increased in high glucose-treated HRMECs. Silencing of NOX4 inhibited high glucose-induced increases in NLRP3 inflammasome and ROS generation, and decreases in the protein levels of ZO-1 and VE-Cad. Over-expression of NOX4 significantly increased the levels of NLRP3 inflammasome-related proteins and ROS generation in HRMECs, and reduced the protein levels of ZO-1 and VE-Cad. Treatment with SDX partly reversed NOX4 over-expression-induced changes. CONCLUSION SDX alleviates hyperglycemia-induced retinal microvascular endothelial injury via inhibiting NOX4/ROS/NLRP3 pathways.     

浅析林业害虫防治的生态调控研究

浅析了林业害虫防治生态调控的理论发展过程,分析了综合使用生物防治、造林技术、抗性品种选育和害虫与天敌动态监测等进行调控林木-害虫-天敌相互关系的措施;最后阐述了害虫防治的目的是将害虫种群控制在经济阀值水平之下,而不是全部消灭.目前对生态系统的认识水平和技术水平,还不能完全实施生态调控,但已在逐步转变,今后有必要加大这方...     

不同播期对水稻秧苗素质影响的研究

通过对不同播种期的秧苗素质的研究,确定最佳的播种时期.结果表明:第1叶鞘高与2叶长呈极显著的正相关(r=0.8201**);秧龄期和株高呈极显著正相关(R=0.9367**).结合2006年4月份气温特点,并通过对第1叶鞘高、2叶长、根数、株高等性状分析,初步确认三江地区水稻最佳播种期是4月10～15日.     

IBA对芍药扦插生根的影响及生根过程中相关酶活性的变化

以芍药‘大富贵’品种为材料, 用1 000、2 000和3 000 mg·L^-1 3个浓度的IBA处理进行扦插对比试验, 研究其生根过程中过氧化物酶( POD) 、多酚氧化酶( PPO) 、吲哚乙酸氧化酶( IAAO) 活性的动态变化。结果表明: IBA处理极显著地提高了插穗的生根率、根数和总根长, 其中以2 000 mg·L ^-1为IBA最佳生根浓度, 将生根周期缩短18 d, 并明显提高了生根质量。在生根过程中, 插穗中POD、PPO活性分别呈现“缓慢上升→下降→急剧上升→下降”和“降→升→降”的变化趋势, 但与对照组相比, 处理组的变化更为剧烈, 峰值出现提前了18 d。IAAO活性在对照和处理中呈现不同的变化趋势: 前者为“升→降→升”, 而后者为“降→降→升”。此外, 高活性的POD、PPO有利于不定根的形成, 低活性的IAAO则促进生根。     

Effect of inhibiting nuclear factor-kappa B on TNF-α-induced expression of angiotensinogen and production of angiotensinⅡ in glomerular mesangial cells

AIM: To study the effect of inhibiting nuclear factor-kappa B (NF-κB) activity on the expression of angiotensinogen (AGT) and the production of angiotensinⅡ (AngⅡ) induced by tumor necrosis factor-α (TNF-α) in glomerular mesangial cells (MCs) of SD rats. METHODS: The MCs of SD rats were isolated and divided into three groups as follows: control; MCs treated with TNF-α, and the MCs treated with TNF-α + pyrrolidinedithiocarbamate (PDTC). The activity of nuclear factor-kappa B was measured by electrophoretic mobility shift assay. The expression of AGT was determined by RT-PCR for mRNA and Western blotting for protein. The concentration of angiotensinⅡ in supernatant was measured by RIA. RESULTS: The NF-κB activity in the MCs treated with TNF-α (20.67±9.14)×102 μg/cell was significantly higher than that in control cells ［(8.25±4.35)×102 μg/cell, P<0.01］ and the MCs treated with TNF-α+PDTC ［(7.20±4.57)×102 μg/cell, P<0.01］, and no significant difference was found between control and the MCs treated with TNF-α＋PDTC (P>0.05). The AGT mRNA level in the MCs treated with TNF-α (0.27±0.05) was higher than that in the control cells (0.20±0.05, P<0.05), and no significant difference was observed when compared with that in the MCs treated with TNF-α＋PDTC (0.22±0.06, P>0.05). The expression of AGT protein in the MCs treated with TNF-α (0.60±0.19) μg/cell was higher than that in the control ［(0.37±0.15)μg/cell, P＜0.05］ and the MCs treated with TNF-α＋PDTC ［(0.37±0.17)μg/cell, P<0.05］, and no significance was found between the MCs treated TNF-α＋PDTC and the control (P>0.05). The AngⅡ level in supernatant of cultured MCs treated with TNF-α ［(9.73±2.38)×10-5 ng·L-1/cell］ was significantly higher than that in the control ［(7.50±1.51)×10-5 ng·L-1/cell, P<0.05］ and in the MCs treated with TNF-α＋PDTC ［(6.94±1.46)×10-5 ng·L-1/cell, P<0.05］, however, the difference between the MCs treated with TNF-α＋PDTC and the control was of no significance (P>0.05). CONCLUSION: TNF-α activates the NF-κB in glomerular MCs, induces the AGT expression and the production of AngⅡ. Inhibition of NF-κB decreases the AGT expression and the production of AngⅡ. Therefore, the effects of TNF-α on AGT and AngⅡ may be mediated by NF-κB.     

Effects of cytokines on renovation of acute renal failure in mouse with bone marrow derived mesenchymal stem cell transplantation

AIM: To observe the effects of cytokines on renovation of acute renal failure (ARF) in mouse with bone marrow derived mesenchymal stem cell (MSC) transplantation. METHODS: ARF animal model was induced in mouse by subcutaneous injection of cisplatin. Mice were randomly assigned into 3 groups: normal control group, ARF group and MSC group. After 24 h cisplatin injection, animals were injected intravenously with MSC in MSC group. Animals were sacrificed at 1 d, 4 d, 7 d, 14 d and 28 d after cisplatin injection. The blood urea nitrogen (BUN) and serum creatinine (Scr) were measured. The renal morphologic changes were scored with Paller’s criterion on hematoxylin and eosin (HE) stained sections. The mRNA and protein expressions of HGF, BMP-7, TNF-α and IL-10 were detected by RT-PCR and immunohistochemistry method. RESULTS: After 4 d of cisplatin injection, the BUN and Scr values in MSC group were significantly lower than those in ARF group (P<0.01). After 7 d and 14 d, the values of BUN and Scr in MSC group were still lower than those in ARF group (P<0.01, P<0.05). The renal morphologic scores of MSC group were also lower than those of ARF group. After 7 d, the expressions of HGF, BMP-7 and IL-10 were higher in MSC group than those in ARF group, the expression of TNF-α in MSC group was lower than that in ARF group. CONCLUSION: MSC promotes the recovery of acute renal failure induced by cisplatin. The mechanism may partly depend on paracrine of growth factor and amelioration of inflammatory.     

被忽视的晚清诗文拓荒者——论胡先骕之诗文评

上个世纪20年代,胡先骕在《学衡》发表多篇关于清代诗词集的评论。他独具慧眼,对赵熙、阮大铖、刘光第等人的研究,皆有开山奠基之功,后世研究多以此为基础,可谓是晚清诗歌研究的拓荒者。在这些文章中,胡先骕强调道德节制、中庸等思想,反对白话文运动,体现了他保守主义的文化观。也正由于胡先骕的文化立场及其他复杂原因,导致其诗歌研究的开拓之功,长期以来未得到应有的重视。