Inhibition of Ang-2 and RGS-5 expression by nanogold results in normalization of vascellum in hepatic tumor

AIM: To observe the vascular normalization effect of nanogold on hepatic tumor by inhibiting angiopoietin-2 (Ang-2), regulator of G-protein signaling-5 (RGS-5) during certain time window. METHODS: H22 cells, the hepatocellular cancer cell line, were subcutaneously injected into the right armpits of 48 BALB/c nude mice. When the size of transplanted tumor reached 3~4 mm, the mice were divided into 2 groups: 36 mice in experiment group and 12 mice in control group. The mice in experimental group underwent injection of nanogold into the tumor once a day, and the mice in control group were injected with normal saline. After continuous treatment with nanogold for 3 days, 7 days and 11 days, the mice were sacrificed, the liver tumors were taken out to measure the size and weight. The expression of Ang-1,Ang-2 and RGS-5 in the tumor was detected by the method of immunohistochemical staining. The normalizing shapes of tumor vessels and the pericytes were observed under electronic microscope. RESULTS: With nanogold treatment for 3 days, 7 days and 11 days, the positive rates of Ang-1 were 16.7％, 50.0% and 16.7%, respectively. The positive rates of Ang-2 were 33.3%, 16.7% and 41.7%, respectively. The expression of Ang-1 in experiment group was higher than that in control group, especially at the 7th day in experiment group. The expression of Ang-2 in experiment group was lower than that in control group (58.3%), especially at the 7th day in experiment group. With nanogold treatment for 3 days, 7 days and 11 days, the positive rates of RGS-5 were 33.3%, 16.7% and 50.0%, respectively. The immature pericyte coverage indexes (IMPI) were 19.6%±4.3%, 32.5%±7.9% and 41.2%±9.1% respectively. At the 7th day in experiment group, the positive rates of RGS-5 and IMPI were lower than those in other experiment groups and control group. After treated with nanogold for 7 days, the pericytes in the parietal wall of the blood vessels in the tumor showed the tendency to grow normally in morphology and were completely covered by endothelial cells. However, the pericytes in the parietal wall of the blood vessels in control group showed differences in size, impaired integrity and only a few of the pericytes covered by endothelial cells. CONCLUSION: During the time window of nangold treatment for 7 days, the chemical can normalize the blood vessels in liver cancer by inhibiting the expression of Ang-2 and RGS-5.     

Establishment and evaluation of rat heart ischemia-reperfusion injury model in vivo

AIM: To establish and evaluate a rat model of heart ischemia-reperfusion injury in vivo. METHODS: Seventy-two male Sprague-Dawley rats weighing(250±50)g were randomly divided into sham operation group(sham), ischemia-reperfusion group(I/R) and normal group. The animals were anesthetized and heparinized. Myocardial ischemia-reperfusion was induced by ligating the left anterior descending coronary artery with "U-shape tube" for 35 min followed by 120 min or 240 min reperfusion in vivo. The heart infarct size was measured by triphenyltetrazolium chloride(TTC) staining. The myocardial cell apoptotic index was determined by the method of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling(TUNEL). Immunohistochemical method was used to detect the expression of Bcl-2 and Bax in rat ischemia myocardium. The blood level of MB isoenzyme of creatine kinase(CK-MB),cardiac troponin I(cTnI),nitric oxide(NO),malondialdehyde(MDA), total superoxide dismutase(T-SOD)and glutathione peroxidase(GSH-Px) were detected after reperfusion for 2 h and 4 h. RESULTS: Compared with normal group and sham group, there were obvious changes of ST-T segment and Q wave in the electrocardiogram of I/R group. The blood level of CK-MB, cTnI, NO, MDA and GSH-Px in I/R group increased(P<0.05,P<0.01) after reperfusion for 2 h and 4 h, and the blood level of T-SOD in I/R group after reperfusion for 2 h and 4 h also increased(P<0.05). The heart infarct size in I/R group was the largest as compared to other groups. Among these groups, the apoptotic index of I/R group was the highest and the Bcl-2/Bax ratio in I/R group decreased(P<0.01).CONCLUSION: The rat model of heart ischemia-reperfusion injury in vivo can be successfully established with the "U-shape tube". There are obviously changes of heart infarct size, blood level of CK-MB, cTnI, NO, MDA, T-SOD and GSH-Px, myocardial apoptotic index and Bcl-2/Bax ratio between I/R rats and control animals.     

多头带绦虫甘肃分离株线粒体NADH脱氢酶亚基Ⅰ基因片段差异分析

应用线粒体DNA中的NADH脱氢酶亚基Ⅰ(ND1)基因作为分子标记,对我国甘肃省景泰和平凉地区10个绵羊源脑多头蚴进行分析,构建系统进化树,分析其种内变异.结果表明,所有多头带绦虫(T.multiceps)分离株均成功扩增出约0.5kb的ND1基因片段.序列分析显示,去除引物序列后多头带绦虫的ND1基因片段长为488bp,可以分为9类,共有22个核苷酸变异位点,变异率为0.20%～2.66%.基于ND1序列的系统进化树表明所有T.multiceps分离株构成1个分支,可分为3个亚群,国内分离株分别处在不同的亚群中.国内分离株中除了与已知的遗传变异型Tm1(AY669089/Tm-JT081204/Tm-JT090331)和Tm3(DQ077820/Tm-JT080526)相同外,还存 在新的遗传变异型(Tm-JT081008/Tm-JT090603/Tm-JT090115-2),独立为一支,与其他分离株亲缘关系较远;表明ND1基因片段适合作为研究T.multiceps分离株种内变异的分子标记.     

发酵乳酸杆菌对肉鸡胰腺和小肠消化酶活性及营养物质消化率的影响

本试验旨在研究添加不同水平的发酵乳酸杆菌(L.fermentum F-6)对肉鸡的胰腺、小肠消化酶活性及营养物质消化率的影响.试验以玉米和大豆粕为主要原料配制饲粮,采用单因子随机试验设计方法,选择1日龄体重相近、健康的爱拔益加(AA)肉鸡240只(雌雄各占l/2),称重后随机分为4个组,1个对照组和3个L.fermen...     

多头带绦虫Tm16与Tm18抗原基因的克隆及原核表达

为原核表达Tml6和Tm18重组蛋白,本研究以自然感染羊源脑多头坳原头节基因组DNA为模板分别扩增Tml6和Tm 18杭原基因全基因片段,测序鉴定后合成其开放阅读框架DNA片段,将基因组序列中的内含子去除并对其稀有密码子进行改造优化,构建重组表达质粒pGEX-Tm 16和pGEX-Tml8.转化大肠杆菌BL21后以IPTG诱导表达,SDS-PAGE分析表达产物并进行纯化.结果显示:在大肠杆菌中表达出带有GST标签的大小约为39.6 ku和39.4 ku的重组蛋白,经谷胱甘肤琼脂糖树脂纯化得到高纯度的可溶性的GST-Tm 16及GST-Tml8重组蛋白,western blot分析表明重组蛋白均能够被兔抗GST单克隆杭体特异性识别.     

保鲜剂对碧桃切枝采后水分及膜稳定性影响

以四因素(青霉素、硝酸银、8-羟基喹咛、蔗糖)三水平进行正交设计,通过外部形态观察和生理指标测定,研究了保鲜剂对碧桃切枝瓶插期间水分状况和膜稳定性的影响。结果表明:试验号7(青霉素900 mg/L+硝酸银68 mg/L+8-羟基喹咛300 mg/L+蔗糖50 g/L)的糖和蛋白质分解慢,丙二醛含量低,水分保持好,对延缓碧桃切枝衰老有明显效果。该处理鲜样质量、糖、蛋白质含量分别比最低者高1.18 g、11.233 mg/g、1.623 mg/g;丙二醛含量比最高者低2.97μmol/g;水分平衡值比最低者少0.58单位。     

佳木斯市周边山区野生彩叶树种资源调查及开发利用分析

通过对佳木斯市周边山区(国家森林公园大亮子河、绿潭、横头山和三家甸等)野生彩叶树种调查分析,确定该区野生彩叶树种属15科34种。有观赏特性及开发利用前途的有色木槭、红瑞木、花楸、水榆花楸和狗枣猕猴桃等11种。重点介绍了各种类的观赏特性,并对佳木斯市开发利用野生彩叶树种资源提出了建议。     

薄皮木种子萌发特性研究

以薄皮木当年种子为试材,研究了相对含水量、基质、盐胁迫和pH值等因素对其种子萌发的影响,以期为薄皮木引种驯化和开发利用提供理论依据.结果表明:基质含水量为26.67％时,发芽率和发芽势最高,分别达到30％和20％;薄皮木种子具有广泛的土壤适应性,以沙子、蛭石混合物(比例1∶1)作萌发基质最为适宜;当NaCl浓度为0.5mol/L时,种子萌发率最高为43.43％;在pH 3.0～11.0范围内均可萌发.     

西北干旱半干旱区耕地面积变化与人口、经济发展的相关关系研究——以甘肃省为例

基于甘肃省1997-2006年耕地十年变化的数据,研究了甘肃省经济发展过程中耕地面积变化的过程,并运用统计分析方法,对近十年来耕地变化与人口以及固定资产投资、人均GDP等经济指标进行相关分析.结果表明:耕地面积与人口呈相反趋势,与固定资产投资、人均GDP、城市化水平呈正" U"型三次曲线相关,即固定资产投资总额、人均G...     

区域经济发展的土地利用及生态安全管理——以宝鸡地区为例

区域社会经济发展与土地利用结构关系密切。文中采用耕地动态度、相对变化率、转移率、新增耕地率、线性回归分析以及洛伦兹曲线等方法,以宝鸡地区为例分析了土地动态变化以及与区域经济发展关系、耕地与粮食匹配关系。结果表明：市区和山区县土地变化率大,川原县区相对较小,耕地变化与经济发展呈高度负相关关系。面对区域经济发展中土地资源出...