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141.
Oleaga A Casais R Balseiro A Espí A Llaneza L Hartasánchez A Gortázar C 《Veterinary parasitology》2011,181(2-4):255-266
Sarcoptic mange, a parasitic skin infection caused by the burrowing mite Sarcoptes scabiei, has been reported in over 100 mammals, including humans. In endangered species, mange causes conservation concerns because it may decimate isolated populations and contribute to extinction. The Iberian Peninsula still maintains one of the largest wolf (Canis lupus) populations in Europe. In Iberia, sarcoptic mange is endemic in red foxes (Vulpes vulpes) and the first confirmed wolf mange cases were recently reported. However, knowledge on S. scabiei in wolves is scarce because of the sampling difficulties inherent to research on scarce species. In order to describe wolf mange epidemiology and to infer conservation implications, this study combined traditional laboratory techniques with the revision of wolf carcass pictures taken by field biologists and original information obtained by camera trapping. A total of 125 necropsies and 8783 camera-trap days allowed insights into wolf mange epidemiology between 2003 and 2010. Living Sarcoptes mites were detected in 19% of the fresh carcasses. Alopecic (delayed) type IV hypersensitive response reactions were observed, while parakeratotic lesions were infrequent. The number of mites isolated per wolf ranged from 1 to 78, and had a negative correlation with the percentage of alopecic skin. No effect by sex on mange prevalence was found. Yearlings showed a lower probability to present mange-compatible lesions than pups or adults. Wolves with mange-compatible lesions had a lower kidney fat index than apparently healthy ones. ELISA testing of 88 sera yielded an antibody prevalence of 20%. Photo-trapping recorded mange-compatible lesions since 2003 with a peak in 2008. The percentage of wolves with mange-compatible lesions registered in camera-traps during 1 year correlated with the percentage of red foxes with lesions in the previous year. This is the first large survey on sarcoptic mange in the Iberian wolf. Necropsy data, with alopecia as the main feature and a slight effect on body condition, and trends derived from camera trapping coincided in showing a rather low prevalence and an apparently stable situation of the disease and its host, suggesting that this parasite is currently not a major threat for this wolf population. However, more information is needed in order to assess the effect of mange on aspects such as pup survival. 相似文献
142.
Pose AG Gómez JN Sánchez AV Redondo AV Rodríguez ER Seguí RM Ramos EM Moltó MP Rodríguez ES Cordero LR Mallón AR Nordelo CB 《Veterinary microbiology》2011,152(3-4):328-337
World Health Organization has a great concern about the spreading of avian influenza virus H5N1. To counteract its massive spread, poultry vaccination is highly recommended together with biosecurity measures. In our study, a recombinant vaccine candidate based on the fusion of extracellular segments of hemagglutinin (HA) H5 of avian influenza virus and chicken CD154 (HACD) is tested with the aim of enhancing humoral and cellular immune responses in chickens. Protein expression was carried out by transducing several mammalian cell lines with recombinant adenoviral vectors. HACD purification was assessed by three distinct purification protocols: immunoaffinity chromatography by elution at acidic pH or with a chaotropic agent and size exclusion chromatography. Humoral and cellular immune responses were measured using the hemagglutination inhibition assay and the semiquantitative real time PCR, respectively. The results showed that humoral response against HACD was significantly higher than the obtained with HA alone after booster (P<0.01, P<0.05). From HACD molecules purified by distinct protocols, only the obtained by size exclusion chromatography generated hemagglutinationin-inhibition activity. IFN-γ levels indicated that cellular immune response was significantly higher with HACD, in its pure or impure form, compared to its counterpart HA (P<0.01). These data demonstrate that HACD is able to significantly enhance humoral and cellular immune responses against HA antigen, which make this fusion protein a promising subunit vaccine candidate against H5N1 virus outbreaks. 相似文献
143.
Cocero MJ Alabart JL Hammami S Martí JI Lahoz B Sánchez P Echegoyen E Beckers JF Folch J 《Reproduction in domestic animals》2011,46(3):463-470
In vitro oocyte maturation can be influenced by oocyte source and maturation media composition. The aim of the present study was to compare the efficiency of a defined in vitro maturation medium (TCM199 supplemented with cysteamine and epidermal growth factor; Cys + EGF) with an undefined medium (TCM199 supplemented with follicle-stimulating hormone and follicular fluid; FSH + FF) for in vitro production (IVP) of ovine embryos, using oocytes obtained by laparoscopic ovum pick-up from FSH-stimulated [n=11; 158 cumulus-oocyte complexes (COCs)] and non-stimulated (n=16; 120 COCs) live ewes, as well as abattoir-derived oocytes (170 COCs). The produced blastocysts were vitrified and some of them were transferred to synchronized recipients. The best and the worst final yields of embryo IVP observed in this study were obtained using oocytes from FSH-stimulated ewes matured in FSH + FF (41.3%; 33/80) and in Cys + EGF (19.2%; 15/78) medium, respectively (p<0.01). No significant differences between both media were attained in the blastocyst development rate or in the final yield of embryo IVP using oocytes from non-stimulated ewes or abattoir-derived oocytes. The overall in vivo survival rate of the transferred vitrified blastocysts was 13.1% (8/61), without significant differences between oocyte sources or maturation media. In conclusion, under the experimental conditions of the present study, TCM199 supplemented with cysteamine and EGF is a convenient defined maturation medium for IVP of embryos from oocytes of live non-stimulated ewes or from oocytes of abattoir-derived ovaries. However, the best final yield of embryo IVP observed in this study was attained when oocytes came from FSH-stimulated donors and TCM199 was supplemented with FSH and follicular fluid. 相似文献
144.
Manuel López-Vicente Noemí Lana-Renault José Maria García-Ruiz Ana Navas 《Journal of Soils and Sediments》2011,11(8):1440-1455
Purpose
Sediment delivery from headwater catchments to reservoirs is a serious threat to reservoir sustainability and is a critical issue in Mediterranean environments where water resources are scarce. In this study we assessed the consequences of two landscape management scenarios (natural vegetation recovery and scrub clearance) on soil erosion and sediment yield. The results were analyzed in relation to predicted and measured rates of soil erosion and sediment yield, with the aim of promoting better management practices. 相似文献145.
Olivero-David R Paduano A Fogliano V Vitaglione P Bastida S González-Muñoz MJ Benedí J Sacchi R Sánchez-Muniz FJ 《Journal of agricultural and food chemistry》2011,59(9):4684-4691
Western diets contain substantial amounts of lipid oxidation products. The effects of fasting status and oil oxidation on short-term digestibility of oxidized fatty acids (ox-FA) and ketolinoleic acids (keto-LA) of sunflower oils were evaluated. Twelve rats were fasted overnight for 3 days, whereas another 12 rats had free access to diet. From day 4, and for 4 days, two groups of rats, nonfasted (NFT) and fasted (FT), received 1 g/100 g body weight of sunflower oil reused from 40 deep-frying processes, and two control groups of rats, nonfasted (NFC) and fasted (FC), received the same amount of fresh oil. Ox-FA and keto-LA were determined 5 h after the last administration in the various gastrointestinal compartments together with the intraintestinal MDA. Oil digestibility was highest in NFC and lowest in FT rats. NFT and FT rats had higher (at least P < 0.05) intraintestinal MDA, ox-FA, and keto-LA than NFC and FC; MDA and keto-LA concentrations correlated with each other (P < 0.05). Ox-FA and keto-LA levels found in the gastric lumen suggest that digestion contributes to the formation of these compounds. Total ox-FA and keto-LA were efficiently absorbed during the first 5 h after test oil administration, but poorly absorbed in the case of fresh oils. Oil alteration influenced the digestibility of these compounds more than fasting, although the digestibility of oxidized oil was significantly affected by fasting. 相似文献
146.
147.
Humus accumulation, humification, and humic acid composition in soils of two post-mining chronosequences after coal mining 总被引:1,自引:0,他引:1
Evgeniy Vasilyevitch Abakumov Tomáš Cajthaml Jiří Brus Jan Frouz 《Journal of Soils and Sediments》2013,13(3):491-500
Purpose
To assess how the rates of humus formation and humification are affected by land use and age of ecosystems, this study investigated soil development in two post-mining chronosequences (spoil heaps formed from open-cast coal mining near Sokolov, Czech Republic). The following characteristics were measured: content, composition, and properties of humic acids; organic carbon (C) and total nitrogen (N) contents; pH; and amorphous iron content.Materials and methods
Two chronosequences were studied. One consisted of unreclaimed spontaneously revegetated spoils (3, 12, 20, and 40 years old). The other consisted of heaps that were reclaimed by planting alder in graded heaps (7, 15, 20, 30, and 40 years old). Humus and iron contents as well as pH were determined, and humic acids were extracted for detailed chemical analyses (C, H, N, 13C-NMR, and pyrolysis mass-spectrometry).Results and discussion
C and N accumulated faster in the reclaimed sites than in the unreclaimed sites; organic matter accumulation results in the decrease of pH in all soils and in spontaneous sites also increase of amorphous iron content. Humic acids (HA) and fulvic acids (FA) increased with the site age in both chronosequences. The CHA/CFA was higher in the reclaimed soils than in the unreclaimed soils, and the CHA/CFA ratio increased with age in the unreclaimed soils. Humic acid aromaticity was higher in the reclaimed site than in the unreclaimed sites but increased with the age of unreclaimed sites. H and O content decreased with age of reclaimed soils. Humification led to an increase in HA caloricity in the reclaimed sites but not in unreclaimed sites because of the influence of residual wax-type substances in the unreclaimed sites. Degree of HA oxidation (ω) for the reclaimed plots was similar to that in typical zonal soils but was highly variable in unreclaimed soils.Conclusions
Relative to spontaneous revegetation in the unreclaimed sites, reclamation increased the rates of humus accumulation, humification, and humus acid transformations. The differences between reclaimed and unreclaimed sites, however, decreased with site age and were very small in 40-year-old sites. These differences correspond to the rapid colonization of the reclaimed sites and the slow colonization of the unreclaimed sites by soil biota. 相似文献148.
Waters of poor quality are often used to irrigate crops in arid and semiarid regions, including the Fars Province of southwest Iran. The UNSATCHEM model was first calibrated and validated using field data that were collected to evaluate the use of saline water for the wheat crop. The calibrated and validated model was then employed to study different aspects of the salinization process and the impact of rainfall. The effects of irrigation water quality on the salinization process were evaluated using model simulations, in which irrigation waters of different salinity were used. The salinization process under different practices of conjunctive water use was also studied using simulations. Different practices were evaluated and ranked on the basis of temporal changes in root-zone salinity, which were compared with respect to the sensitivity of wheat to salinity. This ranking was then verified using published field studies evaluating wheat yield data for different practices of conjunctive water use. Next, the effects of the water application rate on the soil salt balance were studied using the UNSATCHEM simulations. The salt balance was affected by the quantity of applied irrigation water and precipitation/dissolution reactions. The results suggested that the less irrigation water is used, the more salts (calcite and gypsum) precipitate from the soil solution. Finally, the model was used to evaluate how the electrical conductivity of irrigation water affects the wheat production while taking into account annual rainfall and its distribution throughout the year. The maximum salinity of the irrigation water supply, which can be safely used in the long term (33 years) without impairing the wheat production, was determined to be 6 dS m?1. Rainfall distribution also plays a major role in determining seasonal soil salinity of the root zone. Winter-concentrated rainfall is more effective in reducing salinity than a similar amount of rainfall distributed throughout autumn, winter, and spring seasons. 相似文献
149.
The identification of optimal management strategies for a given species is a major challenge of species conservation. It becomes especially challenging when the environmental conditions are expected to change in the future, and the optimal management applied today may differ from the management that is optimal under the changed conditions (e.g. due to climate change).This study evaluates prospect of a rare plant species endemic to semi-natural grasslands in central Europe, Gentianella praecox subsp. bohemica. The number of populations of this species has declined rapidly in the last 60 years; currently, a conservation action plan has been established in the Czech Republic, where most populations of this species occur. This study uses periodic matrix models to compare different management regimes under different scenarios of climate change and to identify the optimal management in each case.Without management, populations of the species are not able to survive. Flowering individuals can occur for a long time after the cessation of management, but the extinction of the population is inevitable within several decades. Without management, even very large populations (1000 flowering individuals) will go extinct in less than 50 years. Total extinction (including seed bank) will follow several years after observation of the last flowering plant. The most suitable management is mowing and disturbance (by harrowing), which is also the best method for restoration of threatened populations. Mowing is less suitable, but it is fully sufficient for large prospering populations. When managed, even small populations (10–15 flowering individuals) are able to survive. When management is applied, future climate change may have a relatively small impact on the probability of survival of the species. Climate change will, however, increase the extinction probability of very small populations. 相似文献
150.
Sherry Kurtz Lloren? Grau-Roma Martí Cortey Maria Fort Fernando Rodríguez Marina Sibila Joaquim Segalés 《Veterinary research》2014,45(1):29
Porcine circovirus type 2 (PCV2) is the essential infectious agent for PCV2-systemic disease (PCV2-SD, formerly known as postweaning multisystemic wasting syndrome) and other pathological conditions. Recent studies indicated antigenic variability amongst different PCV2 isolates and suggested that single amino acid changes within the capsid protein determine differences in the level of neutralization by specific monoclonal antibodies. The objective of the present study was to examine the cross-reactivity of PCV2 antibodies induced in the context of a natural infection against different PCV2 isolates belonging to genotypes PCV2a and PCV2b. Sera taken from several farms from animals of varying health status (PCV2-SD and age-matched healthy pigs and a set of slaughter-aged animals) were assayed for neutralizing activity against four PCV2 isolates from both predominant genotypes (PCV2a and PCV2b) and of differing geographic origins (Europe and North-America). Results showed that most of studied pigs (79 out of 82) contained neutralizing antibodies (NA) able to neutralize all four studied viral strains. Overall, pigs had significantly higher NA titres against PCV2a than against PCV2b (P < 0.001). Accordingly, studied serums were able to better neutralize Burgos390L4 and Stoon-1010 strains (PCV2a) than L-33-Sp-10-54 and MO/S-06 strains (PCV2b) (P < 0.001). No differences between capabilities of seroneutralization of viruses from different geographic origin were observed. Present data suggests that sequence differences between PCV2 isolates translate to functional antigenic differences in viral neutralization in vivo. 相似文献