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71.
Saito KC Bechara GH Nunes ET de Oliveira PR Denardi SE Mathias MI 《Veterinary parasitology》2005,129(3-4):299-311
This study presents the morphology of the ovary, as well as the dynamics of the vitellogenesis process in oocytes of the cattle-tick Boophilus microplus. The ovary of these individuals is of the panoistic type; therefore, it lacks nurse cells. This organ consists of a single tubular structure, continuous, and composed of a lumen delimitated by a wall of small epithelial cells with rounded nuclei. In this tick species, the oocytes were classified into six stages varying from I to VI and according to: cytoplasm appearance and presence of the germ vesicle, yolk granules, and chorion. Oocytes of various sizes and at different developmental stages remain attached to the ovary through a cellular pedicel until completing stage V. Afterwards, they are liberated into the lumen and from there to the exterior. Some oocytes (classified as type VI) showed an atypical appearance indicating that some of the cellular components would be undergoing a degenerative process and/or reabsorption. 相似文献
72.
C. Valente G. Fruganti B. Tesei A. Ciorba P. Cardaras A. Floris E. Bordoni 《Comparative immunology, microbiology and infectious diseases》1988,11(3-4):189-198
The immune response to the K99 was tested in 45 pregnant cows, subcutaneously vaccinated, for protecting the newborn calves. Serological tests were performed in the blood sera of all animals and in the milk and colostrum sera; hemogram, inhibition of the adhesion to the brush border and histological tests were performed. The calves from vaccinated cows survived the experimental infection after the suction of colostrum in spite of the fact that the calves from control dams died with diarrhea. 相似文献
73.
Giovanni Zurlini Norbert Marwan Teodoro Semeraro K. Bruce Jones Roberta Aretano Maria Rita Pasimeni Donatella Valente Christian Mulder Irene Petrosillo 《Landscape Ecology》2018,33(9):1617-1631
Context
Socio-ecological landscapes typically characterized by non-linear dynamics in space and time are difficult to be analyzed using standard quantitative methods, due to multiple processes interacting on different spatial and temporal scales. This poses a challenge to the identification of appropriate approaches for analyzing time series that can evaluate system properties of landscape dynamics in the face of disturbances, such as uncontrolled fires.Objective
The purpose is the application of non-linear methods such as recurrence quantification analysis (RQA) to landscape ecology. The examples concern the time series of burnt and unburnt Mediterranean rangelands, to highlight potential and limits of RQA.Methods
We used RQA together with joint recurrence analysis (JRA) to compare the evolutionary behavior of different land uses.Results
Time series of forests and grasslands in rangelands present both periodic and chaotic components with a rather similar behavior after the fire and clear transitions from less to more regular/predictable dynamics/succession. Results highlight the impacts of fire, the recovery capacity of land covers to pre-burnt levels, and the decay of synchronization towards the previous regime associated with vegetation secondary succession consistent with early successional species.Conclusions
RQA and JRA with their set of indices (recurrence rate: RR, laminarity: LAM, determinism: DET, and divergence: DIV) can represent new sensitive measures that may monitor the adaptive capacity and the resilience of landscapes. However, future applications are needed to standardize the analysis by strengthening the accuracy of this approach in describing the ongoing transformations of natural and man-managed landscapes.74.
75.
Emili Alcoverro Erika Bersan Daniel Sanchez‐Masian Martina Piviani 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2019,48(1):78-82
A 1‐year‐old, female intact Pug dog was presented to the Small Animal Teaching Hospital of the University of Liverpool with a 4‐week history of progressive multifocal intracranial signs. Magnetic resonance imaging (MRI) detected multiple hemorrhagic lesions in the brain. The Baermann and zinc sulfate flotation tests with centrifugation, performed on fecal samples, were positive for lungworm larvae and an antigenic test confirmed Angiostrongylus vasorum infection. Anthelmintic treatment was started with a consequent marked clinical improvement. Seventy days later, the dog was clinically normal, and no larvae were detected on the Baermann test. Repeat MRI of the brain revealed marked improvement of the hemorrhagic lesions. Cerebrospinal fluid analysis (CSF) showed marked eosinophilic pleocytosis, and anthelmintic treatment was restarted. A follow‐up CSF analysis 4 months after the first presentation revealed resolution of the eosinophilic pleocytosis. This is the first case report of marked eosinophilic pleocytosis associated with neural A vasorum infection in a dog. The CSF eosinophilic pleocytosis persisted for several weeks after treatment, even in the absence of concurrent clinical signs and with a negative A vasorum Baermann test. 相似文献
76.
Influence of supraphysiological cortisol manipulation on predator avoidance behaviors and physiological responses to a predation threat in a wild marine teleost fish 下载免费PDF全文
Michael J. LAWRENCE Erika J. ELIASON Jacob W. BROWNSCOMBE Kathleen M. GILMOUR John W. MANDELMAN Lee F.G. GUTOWSKY Steven J. COOKE 《Integrative zoology》2018,13(2):206-218
The stress axis in teleost fish attempts to maintain internal homeostasis in the face of allostatic loading. However, stress axis induction has been associated with a higher predation rate in fish. To date, the physiological and behavioral factors associated with this outcome are poorly understood. The purpose of the present study was to investigate the impact of experimental cortisol elevation on anti‐predator behavior and physiological responses to predator presence. We hypothesized that semi‐chronic cortisol elevation would increase susceptibility to predation by increasing stress‐induced risk‐taking behaviors. To test this hypothesis, schoolmaster snapper were given cocoa butter implants without cortisol (sham) or with cortisol (50 mg/kg body weight) and tethered to cover. Fish were exposed to either a lemon shark or control conditions for 15‐min. Space use and activity were recorded throughout and fish were terminally sampled for blood. Cortisol implantation, relative to shams, resulted in higher blood glucose and plasma cortisol concentrations with a lower plasma lactate concentration. Shark exposure, relative to controls, elicited higher blood glucose and lactate concentrations but had no effect on plasma cortisol concentration. No interactions were detected between shark exposure and cortisol treatment for any physiological trait. Behavioral metrics, including shelter use and activity, were unaffected by either cortisol implantation or shark exposure. Physiological responses to cortisol implantation likely resulted from enhanced gluconeogenic activity, whereas alterations under predator exposure may have been the product of catecholamine mobilization. Further work should address context‐specific influences of stress in mediating behavioral responses to predation. 相似文献
77.
In this paper, multilevel logistic models which take into account the multilevel structure of multi-site pig production were used to estimate the variances between pigs produced in Danish multi-site pig production facilities regarding seroconversion to Actinobacillus pleuropneumoniae serotype 2 (Ap2) and Mycoplasma hyopneumoniae (Mh). Based on the estimated variances, three newly described computational methods (model linearisation, simulation and linear modelling) and the standard method (latent-variable approach) were used to estimate the correlations (intra-class correlation components, ICCs) between pigs in the same production unit regarding seroconversion. Substantially different values of ICCs were obtained from the four methods. However, ICCs obtained by the simulation and the model linearisation were quite consistent. Data used for estimation were collected from 1161 pigs from 429 litters reared in 36 batches at six Danish multi-site farms chronically infected with the agents. At the farms, weaning age was 3-4.5 weeks, after which batches of pigs were reared using all-in/all-out management by room. Blood samples were collected shortly before: weaning, transfer from weaning-site to finishing-site, and sending the first pigs in the batch for slaughter (third sampling). Few pigs seroconverted at the weaning-sites, whereas considerable variation in seroconversion was observed at the finishing-sites. Multilevel logistic models (initially including four levels: farm, batch, litter, pig) were used to decompose the variation in seroconversion at the finishing-site. However, there was essentially no clustering at the litter level-leading to the use of three-level models. In the case of Ap2, clustering within batch was so high that the data eventually were reduced to two levels (farm, batch). For seroconversion to Ap2, ICC between pigs within batches was approximately 90%, whereas the ICC between pigs within batches for Mh was approximately 40%. This indicates that the possibility for Mh to spread between pigs within batches is lower than for Ap2. The diversity in seroconversion between batches within the same farm was large for Ap2 (ICC approximately 10%), whereas there was a relative strongly ICC (approximately 50%) between batches for Mh. This indicates that the transmission of Mh is more consistent within a farm, whereas the presence of Ap2 varies between batches within a farm. 相似文献
78.
Erika Lück Joerg Ruehlmann 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(5):438-447
Abstract Swedish long-term soil fertility experiments were used to investigate the effect of texture and fertilization regime on soil electrical conductivity. In one geophysical approach, fields were mapped to characterize the horizontal variability in apparent electrical conductivity down to 1.5 m soil depth using an electromagnetic induction meter (EM38 device). The data obtained were geo-referenced by dGPS. The other approach consisted of measuring the vertical variability in electrical conductivity along transects using a multi-electrode apparatus for electrical resistivity tomography (GeoTom RES/IP device) down to 2 m depth. Geophysical field work was complemented by soil analyses. The results showed that despite 40 years of different fertilization regimes, treatments had no significant effects on the apparent electrical conductivity. Instead, the comparison of sites revealed high and low conductivity soils, with gradual differences explained by soil texture. A significant, linear relationship found between apparent electrical conductivity and soil clay content explained 80% of the variability measured. In terms of soil depth, both low and high electrical conductivity values were measured. Abrupt changes in electrical conductivity within a field revealed the presence of ‘deviating areas’. Higher values corresponded well with layers with a high clay content, while local inclusions of coarse-textured materials caused a high variability in conductivity in some fields. The geophysical methods tested provided useful information on the variability in soil texture at the experimental sites. The use of spatial EC variability as a co-variable in statistical analysis could be a complementary tool in the evaluation of experimental results. 相似文献
79.
Silvia Preziuso Fulvio Laus Aurora Romero Tejeda Carlo Valente Vincenzo Cuteri 《Journal of veterinary science (Suw?n-si, Korea)》2010,11(1):67-72
Streptococcus (S.) dysgalactiae subsp. equisimilis is responsible for severe diseases in humans, including primary bacteraemia, pneumonia, endocarditis, and toxic shock syndrome. Infection in some animal species can also occur, although a few studies have looked into cross-species infectivity. In horses, S. equisimilis is generally considered infrequent or opportunistic, but has recently been isolated from cases of strangles-like disease. Rapid and sensitive diagnostic techniques could enable epidemiological studies and effective investigation of outbreaks involving these bacteria. In this study, PCR protocols previously described in cattle and in humans to detect the species S. dysgalactiae and the subspecies equisimilis were evaluated to detect specific sequences in equine samples. For this purpose, 99 monolateral nasal swabs were collected from horses from stud farms with a history of S. equisimilis infection and were tested blindly by bacteriological isolation and by single and duplex PCR. DNA for PCR was extracted both from the colonies grown on agar media and from enrichment broth aliquots after incubation with nasal swab samples. S. equisimilis was identified by bacteriological isolation in 23 out of 99 swab samples, and PCR assays on these colonies were fully concordant with bacteriological identification (kappa statistic = 1.00). In addition, PCR of the enrichment broth aliquots confirmed the bacteriological results and detected S. equisimilis in 6 samples more than the bacteriological examination (kappa statistic = 0.84). The PCR protocols appeared to be reliable for the rapid identification of S. equisimilis in equine nasal swab samples, and could be useful for microbiological diagnosis. 相似文献