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21.
C. Eliana Gattullo Andreina Traversa Nicola Senesi Elisabetta Loffredo 《Water, air, and soil pollution》2012,223(9):6035-6044
The objective of this study was to assess the removal of the endocrine disruptor 4-nonylphenol (NP) at a concentration of 1?mg?L?1 by ryegrass and radish during germination and growth. The decontamination process was evaluated in water only or in water containing two organic fractions, a soil humic acid (HA) and a river natural organic matter (NOM) at concentrations of 10 and 200?mg?L?1. The addition of these fractions aimed to simulate the organic content of real aqueous systems. At the end of germination and growth, residual NP was measured by chromatographic analysis. Also, NP phytotoxicity was evaluated during germination. In germination experiments, NP in water only was not toxic for ryegrass and radish which removed, respectively, 37 and 51?% of the initial NP added. In water added with HA or NOM at both doses, in general, NP did not influence or inhibited seed germination. Both doses of HA in water promoted the removal of NP by germinating seeds, whereas NOM exerted differentiated results in the two species as a function of the dose applied. After 2?days of growth, in all treatments both species almost completely removed NP and accumulated a very little fraction of product. This study demonstrated that both ryegrass and radish possess a relevant capacity to remove the endocrine disruptor NP from water also in the presence of different organic fractions, thus suggesting their use in the decontamination of real aquatic systems. 相似文献
22.
Custódio L Escapa AL Fernandes E Fajardo A Aligué R Alberício F Neng N Nogueira JM Romano A 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(1):78-84
This work aimed to evaluate the phytochemical content and to determine the antioxidant and cytotoxic activities of methanol
extracts of the carob tree (Ceratonia siliqua L.) germ flour. The extracts were rich in phenolic compounds, had considerable antioxidant activity, and reduced the viability
of cervical (HeLa) cancer cells. The chemical content and the biological activities of the extracts were significantly affected
by gender and cultivar. Female cultivar Galhosa had the highest levels of phenolic compounds, and the highest antioxidant
activity. Extracts from the hermaphrodite trees and from the female cultivars Galhosa and Costela/Canela exhibited the highest
cytotoxic activity. The most abundant compound was theophylline. The phenolic content was correlated to both antioxidant and
cytotoxic activities. Our findings provide new knowledge about the health implications of consuming food supplemented with
carob germ flour. 相似文献
23.
Eliana L. Tassi Francesca Pedron Meri Barbafieri 《Water, air, and soil pollution》2011,216(1-4):275-287
Assessment of native plants and laboratory-scale phytoextraction tests are fundamental and preliminary steps in checking the feasibility and practice of low-cost and low-impact phytoremediation. In this study, we investigated the absorption of B by plants as a tool to remove boron in sediments from different areas of the Cecina River basin in Tuscany, Italy. The investigation was performed analyzing total and available B fraction in sediment samples as well as the B content in different tissues of native plants colonizing the contaminated areas. In laboratory scale, a phytoextraction screening test was performed. Selected high biomass crops (Brassica juncea, Zea mays, and Helianthus annuus) were evaluated in the most contaminated sample in two consecutive growing cycles. Results from field survey showed no hyperaccumulator native plant was present in the investigated areas although, high accumulation levels were found in native species from Bulera dump (Rumex crispus??259 mg?kg?1 and Poa spp??203 mg?kg?1). Results from laboratory phytoextraction tests showed a higher ability of B. juncea which removed about 18.5 mg?B?kg?1 sediment in after the two consecutive growing cycles, representing on the whole 45% of the initial available B fraction. The sediment characteristics affected by the phytoextraction processes were also discussed. 相似文献
24.
Stefania Uccheddu Eliana Pintus Jose Julian Garde Lidia Fleba Marco Muzzeddu Flavia Pudda Luisella Bogliolo Alessandro Strina Stefano Nieddu Sergio Ledda 《Reproduction in domestic animals》2020,55(10):1294-1302
Habitat degradation leads to small and fragmented populations, lower genetic variability and fertility overtime. Assisted reproductive techniques represent important tools to cope with the dramatic loss of biodiversity. Fallow deer (Dama dama), beyond its high commercial value and wide distribution, may represent the most suitable model to study endangered cervids. In this study, oocytes were recovered post-mortem from fallow deer during the breeding and no breeding seasons and were in vitro matured (IVM). The ability of cryopreserved thawed sperm samples recovered by electroejaculation from four adult males was tested by in vitro fertilization of IVM oocytes. The number of oocytes collected per ovary did significantly vary across seasons from 6.2 ± 0.92 during breeding season to 10.4 ± 1.26 during no breeding season (p = .006). Oocytes collected during the breeding season showed higher in vitro fertilization rate compared to the no breeding season (p = .045). However, no embryos reached the blastocyst stage. Semen samples obtained by electroejaculation were successfully cryopreserved, although the cryopreservation process negatively affected most kinetic parameters, mainly at 2 hr post-thawing. Moreover, the percentage of rapid spermatozoa significantly decreased between fresh samples and at 2 hr post-thawing, whereas the percentage of slow spermatozoa increased across the same period (p < .05). Our study provides the logistic steps for the application of assisted reproductive techniques in fallow deer and might be of great interest for genetic resource bank planning. 相似文献
25.
Adelaide Almeida ?ngela Cunha Newton C.M. Gomes Eliana Alves Liliana Costa Maria A.F. Faustino 《Marine drugs》2009,7(3):268-313
Owing to the increasing importance of aquaculture to compensate for the progressive worldwide reduction of natural fish and to the fact that several fish farming plants often suffer from heavy financial losses due to the development of infections caused by microbial pathogens, including multidrug resistant bacteria, more environmentally-friendly strategies to control fish infections are urgently needed to make the aquaculture industry more sustainable. The aim of this review is to briefly present the typical fish farming diseases and their threats and discuss the present state of chemotherapy to inactivate microorganisms in fish farming plants as well as to examine the new environmentally friendly approaches to control fish infection namely phage therapy and photodynamic antimicrobial therapy. 相似文献
26.
Jordão RS Ribeiro CP Pituco EM Okuda LH Del Fava C Stefano Ed Filho MM Mehnert DU 《Research in veterinary science》2011,91(2):311-315
Bovine Viral Diarrhea Virus (BVDV) is widespread in cattle in Brazil and research shows its large antigenic variability. Available vaccines are produced with virus strains isolated in other countries and may not be effective. In this study, inactivated vaccines containing the Brazilian BVDV-Ib IBSP11 isolate were developed and tested on 6 groups of 10 guinea pigs (Cavia porcellus). Animals in groups A and C received an aqueous vaccine (aluminum hydroxide); B and D groups received an oily vaccine (Montanide ISA50); Group E positive-control animals were given an imported commercial vaccine with BVDV-Ia Singer; Group F animals were sham vaccinated (negative control). Groups A, B and E received two doses, and Groups C and D, three, every 21 days. Twelve blood samples were taken, at 21-day intervals over 231 days, and evaluated for antibody titer through virus-neutralization (VN), using a homologous strain (IBSP11), and a heterologous strain (BVDV-Ia NADL). Most animals, 42 days following the first dose, seroconverted to both strains and, after the second dose, there was a significant increase of titers in all groups. The oily formulation induced greater response after the third administration. This increase was not observed with the aqueous vaccines, regardless of the virus used in the VN. Antibody decline was more rapid in animals that received aqueous vaccines. The results showed the importance of studying the influence of endemic strains of commercial vaccines, to improve the efficacy of BVD vaccination. Use of the endemic strain in vaccine formulation presented promising results, as well as the use of guinea pigs as a laboratory model. 相似文献
27.
Eliana Guedes Stehling Tatiana Amabile Campos Marcelo Brocchi Vasco Ariston de Carvalho Azevedo Wanderley Dias da Silveira 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(1):75-83
An Escherichia coli strain (SEPT13) isolated from the liver of a hen presenting clinical signs of septicaemia had a LD50 of 4.0 × 105 CFU/ml in one-day-old chickens, expressed Ia, Ib, E1, E3, K and B colicins and aerobactin. The strain was ampicillin and streptomycin resistant, and found to have fimA, csgA and tsh DNA related sequences; it could adhere to and invade HEp-2 and tracheal epithelial cells, expressed fimbriae (observed by electron microscopy), and had five plasmids of 2.7, 4.7, 43, 56, and 88 MDa. Transposon mutagenesis of strain SEPT13, with transposon TnphoA, resulted in a mutant strain named ST16 that had a LD50 of 1.2 × 1012 CFU/ml. All other biological characteristics of strain ST16 were the same as those detected for strain SEPT13 except for the migration of an 88 MDa plasmid to the 93 MDa position indicating the insertion of the transposon into the 88 MDa plasmid. The 93 MDa plasmid of strain ST16 was transferred, by electroporation assay, to non-pathogenic receptor strains (E. coli strains K12 MS101 and HB101), resulting in transformant strains A and B, respectively. These strains exhibited adhesion properties to in vitro cultivated HEp-2 cells but did not have the capacity for invasion. The adherence occurred despite the absence of fimbriae; this finding suggests that the 88 MDa plasmid has afimbrial adhesin genes. 相似文献
28.
Wildemann Paula Gava Danielle Sfaciotte Ricardo Antônio Pilegi Melo Fernanda Danielle Ferraz Sandra Maria da Costa Ubirajara Maciel Vaz Eliana Knackfuss 《Tropical animal health and production》2018,50(3):671-675
Tropical Animal Health and Production - Yersinia enterocolitica is a foodborne pathogen and pigs are the main reservoir of it in their tonsils. As Brazil is a large producer and exporter of pork... 相似文献
29.
Gregorio Iraola Martín Hernández Lucía Calleros Fernando Paolicchi Silvia Silveyra Alejandra Velilla Luis Carretto Eliana Rodríguez Ruben Pérez 《Journal of veterinary science (Suw?n-si, Korea)》2012,13(4):371-376
Campylobacter (C.) fetus (epsilonproteobacteria) is an important veterinary pathogen. This species is currently divided into C. fetus subspecies (subsp.) fetus (Cff) and C. fetus subsp. venerealis (Cfv). Cfv is the causative agent of bovine genital Campylobacteriosis, an infectious disease that leads to severe reproductive problems in cattle worldwide. Cff is a more general pathogen that causes reproductive problems mainly in sheep although cattle can also be affected. Here we describe a multiplex PCR method to detect C. fetus and differentiate between subspecies in a single step. The assay was standardized using cultured strains and successfully used to analyze the abomasal liquid of aborted bovine fetuses without any pre-enrichment step. Results of our assay were completely consistent with those of traditional bacteriological diagnostic methods. Furthermore, the multiplex PCR technique we developed may be easily adopted by any molecular diagnostic laboratory as a complementary tool for detecting C. fetus subspecies and obtaining epidemiological information about abortion events in cattle. 相似文献