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71.
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Carvalho Camila Aparecida Pozza Edson Amplio de Souza Paulo Estevo Pozza Adlia Aziz Alexandre de Resende Mrio Lcio Vilela Porto Antnio Carlos Mota Balieiro Andr Augusto Ferreira Perez Cristian David Plaza dos Santos Botelho Deila Magna 《European journal of plant pathology / European Foundation for Plant Pathology》2022,163(3):767-774
European Journal of Plant Pathology - Brown eye spot (Cercospora coffeicola) is one of the main diseases of coffee (Coffea arabica L.) and represents a serious phytosanitary problem for the crop.... 相似文献
73.
Silva Adriene Aparecida Pereira Fernanda Aparecida Castro de Souza Elaine Aparecida de Oliveira Denilson Ferreira Nobre Danúbia Aparecida Costa Macedo Willian Rodrigues Silva Geraldo Humberto 《European journal of plant pathology / European Foundation for Plant Pathology》2022,163(4):865-874
European Journal of Plant Pathology - Essential oils (EOs) of Ocimum gratissimum and Syzygium aromaticum and eugenol were applied to common bean seeds harvested from plants with anthracnose... 相似文献
74.
de Pádua Joelma Aparecida Rabelo Rocha Lucas Fernandes Brandão Murilo Malveira de Almeida Vieira Fábio de Carvalho Dulcinéia 《Genetic Resources and Crop Evolution》2021,68(6):2483-2494
Genetic Resources and Crop Evolution - Eremanthus erythropappus (DC.) MacLeish, commonly known as candeia, is a threatened tree species that occurs in Seasonal Semideciduous Forests in Brazil. The... 相似文献
75.
The development of methods to produce embryos in vitro in the horse has been delayed compared with other domestic species. Oocytes can be collected from excised ovaries or from the small or preovulatory follicles of live mares. Intracytoplasmic sperm injection is the only reliable method to fertilize equine oocytes in vitro. Intracytoplasmic sperm injection-produced embryos can be transferred into the oviducts of recipient mares or cultured to the morula or blastocyst stage of development for nonsurgical embryo transfers into recipients' uteri. Embryos cultured in vitro have some morphological differences compared with embryos collected from the mares' uteri. Most notably, the embryonic capsule does not form in culture, and the zona pellucida fails to expand completely. However, embryo produced in vitro can result in viable pregnancies and healthy offspring. 相似文献
76.
Chronic exposure to ultraviolet (UV) light promotes the breakdown of collagen in the skin and disrupts the extracellular matrix (ECM) structure, leading to skin wrinkling. Pacific whiting (Merluccius productus) is a fish abundant on the Pacific coast. In the current study, we investigated the anti-wrinkle effect of hydrolysate from Pacific whiting skin gelatin (PWG) in UVB-irradiated human dermal fibroblasts and the molecular mechanisms involved. PWG effectively restored type 1 procollagen synthesis reduced by UVB-irradiation. Also, we found that PWG inhibited collagen degradation by inhibiting MMP1 expression. Furthermore, PWG decreased cytokines TNF-α, IL-6, and IL-1β associated with inflammatory responses and increased antioxidant enzymes, HO-1, SOD, GPx, CAT, and GSH content, a defense system against oxidative stress. In terms of molecular mechanisms, PWG increased collagen synthesis through activating the transforming growth factor β (TGF-β)/Smad pathway and decreased collagen degradation through inhibiting the mitogen-activated protein kinases/activator protein 1 (MAPK/AP-1) pathway. It also suppressed the inflammatory response through suppressing the nuclear factor-κB (NF-κB) pathway and increased antioxidant enzyme activity through activating the nuclear factor erythroid 2/heme oxygenase 1 (Nrf-2/HO-1) pathway. These multi-target mechanisms suggest that PWG may serve as an effective anti-photoaging material. 相似文献
77.
Abnormal coagulation factor VIII transcript in a Tennessee Walking Horse colt with hemophilia A 下载免费PDF全文
Elaine M. Norton Anne A. Wooldridge Allison J. Stewart Layla Cusimano Dean D. Schwartz Calvin M. Johnson Mary K. Boudreaux Pete W. Christopherson 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2016,45(1):96-102
Hemophilia A is an X‐chromosome‐linked disorder caused by a deficiency in factor VIII (FVIII). Although foals have been diagnosed with hemophilia A based on deficiency in FVIII activity, causative gene mutations have not been identified. The genomic DNA and cDNA encoding FVIII of a Tennesee Walking Horse colt affected with hemophilia A and the genomic DNA of his dam and a normal unrelated horse were analyzed with no splice site or coding sequence abnormalities identified in any of the horses. Polymerase chain reactions (PCR) were then performed on hepatic cDNA from the affected colt and an unrelated normal horse, and no product was obtained for the sequence between and including exon 1 and exon 2 in the affected colt. Based on these results, suspected mutations were identified in the noncoding region of FVIII (intron 1), and genomic sequencing of intron 1 in the dam and the affected colt suggested maternal inheritance. 相似文献
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Cheryl A. London Heather Gardner Shaying Zhao Deborah W. Knapp Sagar M. Utturkar Dawn L. Duval Melissa R. Chambers Elaine Ostrander Jeffrey M. Trent Gina Kuffel 《Veterinary and comparative oncology》2023,21(4):565-577
Pet dogs develop spontaneous cancers at a rate estimated to be five times higher than that of humans, providing a unique opportunity to study disease biology and evaluate novel therapeutic strategies in a model system that possesses an intact immune system and mirrors key aspects of human cancer biology. Despite decades of interest, effective utilization of pet dog cancers has been hindered by a limited repertoire of necessary cellular and molecular reagents for both in vitro and in vivo studies, as well as a dearth of information regarding the genomic landscape of these cancers. Recently, many of these critical gaps have been addressed through the generation of a highly annotated canine reference genome, the creation of several tools necessary for multi-omic analysis of canine tumours, and the development of a centralized repository for key genomic and associated clinical information from canine cancer patients, the Integrated Canine Data Commons. Together, these advances have catalysed multidisciplinary efforts designed to integrate the study of pet dog cancers more effectively into the translational continuum, with the ultimate goal of improving human outcomes. The current review summarizes this recent progress and provides a guide to resources and tools available for comparative study of pet dog cancers. 相似文献
80.
Andr Moreira Souza Rodrigo de Andrade Santos Weigert Elaine Parros Machado de Sousa Lucas Tassoni Andrietta Ricardo Vieira Ventura 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2022,139(1):100-112
The objective of our study was to provide practical directions on the storage of genomic information and novel phenotypes (treated here as unstructured data) using a non-relational database. The MongoDB technology was assessed for this purpose, enabling frequent data transactions involving numerous individuals under genetic evaluation. Our study investigated different genomic (Illumina Final Report, PLINK, 0125, FASTQ, and VCF formats) and phenotypic (including media files) information, using both real and simulated datasets. Advantages of our centralized database concept include the sublinear running time for queries after increasing the number of samples/markers exponentially, in addition to the comprehensive management of distinct data formats while searching for specific genomic regions. A comparison of our non-relational and generic solution, with an existing relational approach (developed for tabular data types using 2 bits to store genotypes), showed reduced importing time to handle 50M SNPs (PLINK format) achieved by the relational schema. Our experimental results also reinforce that data conversion is a costly step required to manage genomic data into both relational and non-relational database systems, and therefore, must be carefully treated for large applications. 相似文献