全文获取类型
收费全文 | 1166篇 |
免费 | 48篇 |
国内免费 | 1篇 |
专业分类
林业 | 68篇 |
农学 | 36篇 |
基础科学 | 10篇 |
178篇 | |
综合类 | 163篇 |
农作物 | 94篇 |
水产渔业 | 112篇 |
畜牧兽医 | 468篇 |
园艺 | 28篇 |
植物保护 | 58篇 |
出版年
2023年 | 9篇 |
2022年 | 8篇 |
2021年 | 8篇 |
2020年 | 19篇 |
2019年 | 25篇 |
2018年 | 24篇 |
2017年 | 20篇 |
2016年 | 22篇 |
2015年 | 16篇 |
2014年 | 29篇 |
2013年 | 45篇 |
2012年 | 43篇 |
2011年 | 81篇 |
2010年 | 53篇 |
2009年 | 50篇 |
2008年 | 82篇 |
2007年 | 72篇 |
2006年 | 80篇 |
2005年 | 67篇 |
2004年 | 64篇 |
2003年 | 61篇 |
2002年 | 55篇 |
2001年 | 4篇 |
2000年 | 4篇 |
1999年 | 5篇 |
1998年 | 17篇 |
1997年 | 13篇 |
1996年 | 12篇 |
1995年 | 7篇 |
1994年 | 9篇 |
1993年 | 16篇 |
1992年 | 9篇 |
1991年 | 17篇 |
1990年 | 14篇 |
1989年 | 14篇 |
1988年 | 11篇 |
1987年 | 10篇 |
1986年 | 9篇 |
1985年 | 6篇 |
1984年 | 12篇 |
1983年 | 9篇 |
1982年 | 4篇 |
1981年 | 4篇 |
1980年 | 9篇 |
1979年 | 9篇 |
1978年 | 13篇 |
1976年 | 4篇 |
1975年 | 4篇 |
1974年 | 5篇 |
1966年 | 4篇 |
排序方式: 共有1215条查询结果,搜索用时 15 毫秒
91.
This paper presents the findings of initial investigation of the role of Spartina alterniora Loisel. in the movement of chlorinated hydrocarbons. 14C-polychlorinated biphenyls and technical Aroclor® 1254 were used for the determinations. The findings demonstrate that Spartina accumulates 14C-radioactivity from sand and organic mud soil systems treated with 14C-PCB's. These findings also demonstrate that this plant has the capacity to accumulate 14C-radioactivity to levels elevated above that in the treated soil. Other experiments with Aroclor® 1254 demonstrate that cogeners of Aroclor® 1254 are present in Spartina stem and root extracts. These experiments also suggest that the chlorinated hydrocarbon uptake process is modulated by the plant and that this results in selective cogener uptake. The selectivity appears to be for the lesser chlorinated components of the commercial mixture. Overall, this study suggests that Spartina may act as an initial vector for mobilization of sediment bound chlorinated hydrocarbons to the estuarine food chain. 相似文献
92.
Michael J. Murray Fabio del Piero Stuart C. Jeffrey Michael S. Davis Martin O. Furr Edward J. Dubovi John A. Mayo 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1998,12(1):36-41
Of 17 foals born on a Thoroughbred breeding farm between March and April 1995, infection with equine herpesvirus type 1 (EHV-1) was associated with neonatal morbidity in 5 foals, 3 of which died or were euthanized. Morbidity and mortality were associated with pulmonary inflammation, and EHV-1 was identified in the lungs of the 3 foals that died. All neonatal EHV-1 infections occurred in foals of mares housed in the same pasture and barn. No other clinical manifestations of EHV-1 infection (eg, abortion, neurologic disease, or respiratory disease) occurred during this outbreak. Three foals were treated with acyclovir (1 died, 2 survived), which may have influenced the clinical outcome in the surviving foals. 相似文献
93.
Erik R. Wisner D V M Dominique Penninck DVM David S. Biller DVM Edward C. Feldman DVM Christiana Drake PhD Thomas G. Nyland DVM 《Veterinary radiology & ultrasound》1997,38(6):462-466
The purpose of this study was to assess the diagnostic utility of parathyroid ultrasonography to differentiate causes of hypercalcemia in dogs. We analyzed qualitative and quantitative ultrasound imaging findings and clinical pathology data from 33 dogs that underwent parathyroid ultrasound examination as part of the diagnostic evaluation for hypercalcemia. Diagnoses of the diseases causing hypercalcemia included parathyroid carcinoma (n = S), parathyroid adenoma (n = 15), parathyroid adenomatous hyperplasia (n = 6), chronic renal insufficiency (n = 3), and hypercalcemia of malignancy (n = 4). All parathyroid lesions were round or oval and hypoechoic compared with surrounding thyroid parenchyma. Adenomatous and adenocarcinomatous glands were 4 mm or larger in longest linear measurement on US examination and were statistically significantly larger than hyperplastic glands. (p < 0.001) Linear measurements of parathyroid glands acquired at the time of ultrasound examination correlated well with direct siz determination after surgical excision. (r2 = 0.9, p < 0.0001) Parathyroid lesions <4 mm are highly suspicious for parathyroid adenoma or carcinoma, while US lesions <4 mm most likely represent primary adenomatous hyperplasia or secondary parathyroid hyperplasia. Parathyroid size estimation from ultrasound examination is an accurate predictor of true size. 相似文献
94.
Andrzej K Siwicki Zdzisaw Zak John C Fuller Jr Steven Nissen Sylwia Trapkowska Edward Gbski Agata Kowalska Krzytof Kazu & Elbieta Terech-Majewska 《Aquaculture Research》2006,37(2):127-131
Studies have shown that in both in vitro and in vivo tests, β‐hydroxy‐β‐methylbutyrate (HMB) increases the nonspecific cellular and humoral immune response and protection against diseases in animals. The present study examines the influence of HMB on nonspecific humoral defense mechanisms and protection against furunculosis in pikeperch (Sander lucioperca). β‐hydroxy‐β‐methylbutyrate was fed in a pelleted ration of 50 mg kg?1 feed day?1 for 4 weeks. Blood was drawn from 12 HMB‐fed and control‐fed pikeperch. The lysozyme and ceruloplasmin activities in the plasma, total immunoglobulin (Ig) levels, and total serum protein were analysed prior to and then after 2 and 4 weeks of HMB ingestion. After 4 weeks of HMB ingestion, a challenge test was performed by injecting the fish with live pathogenic Aeromonas salmonicida bacteria. β‐hydroxy‐β‐methylbutyrate at a dose of 50 mg kg?1 feed resulted in a statistically significant (P<0.05) increase in the lysozyme activity of the plasma, total Ig, and serum protein levels. Additionally, reduced mortality (40%) after the in vivo challenge with pathogenic A. salmonicida suggested that HMB‐activated nonspecific protection against furunculosis in pikeperch. 相似文献
95.
Mariusz Szymczak Edward Kołakowski Katarzyna Felisiak 《Journal Of Aquatic Food Product Technology》2013,22(6):566-581
This study investigated the effect of acetic acid concentrations from 3 to 8% on the physicochemical and sensory quality of marinated Atlantic and Baltic herring from fresh and frozen-thawed material. The increased concentration of acetic acid was found to exert a strong impact on decreased sensory scores, weight yield, and pH value as well as on an increased brightness parameter of meat. In turn, moisture, protein hydrolysis products (PHB), total volatile bases (TVB) content, and hardness parameter were affected by the higher addition of acid to a lesser extent. Enhanced protein hydrolysis was observed only at acetic acid concentration of 5% or 5–8%, whereas there was a decrease in the content of TVB only at an acid concentration of up to 4–5%. The marinated frozen-thawed fillets, compared to the fresh ones, required a greater addition of acid in the brine, by 1.5%, to reach a similar pH value. The freezing of fish contributed also to a decrease of PHB and free hydroxyproline, along with an increased concentration of acetic acid. The marinades from frozen-thawed herring contained more TVB, had worse color, and hardness parameters, as well as less yield. 相似文献
96.
Rasa Jomantiene Yan Zhao Ing-Ming Lee Robert Edward Davis 《European journal of plant pathology / European Foundation for Plant Pathology》2011,130(1):97-107
Phytoplasmas infecting sour cherry and lilac in Lithuania were found to represent two lineages related to clover phyllody
phytoplasma (CPh), a subgroup 16SrI-(R/S)C (formerly 16SrI-C) strain exhibiting rRNA interoperon sequence heterogeneity. 16S
rDNAs amplified from the cherry bunchy leaf (ChBL) and lilac little leaf (LcLL) phytoplasmas were identical or nearly identical
to those of operon rrnA and operon rrnB, respectively, of CPh. There was no evidence of 16S rRNA interoperon sequence heterogeneity in either LcLL or ChBL phytoplasma.
Based on collective RFLP patterns of 16S rDNA, ChBL was classified in subgroup 16SrI-R, and LcLL was classified in new subgroup
16SrI-S. The ribosomal protein (rp) gene sequences from LcLL phytoplasma were identical to those of CPh, and strain LcLL was
classified in rp subgroup rpI-C. By contrast, rp gene sequences from ChBL phytoplasma differed from those of subgroup rpI-C;
based on RFLP patterns of rp gene sequences, ChBL was classified in new rp subgroup rpI-O. Single nucleotide polymorphisms
(SNPs), designated here by a new SNP convention, marked members of rp subgroup rpI-C, and distinguished LcLL and CPh from
ChBL and other non-rpI-C phytoplasmas in group 16SrI. The results raise questions concerning phytoplasma biodiversity assessment
based on rRNA genes alone and encourage the supplemental use of a single copy gene in phytoplasma identification and classification,
while drawing attention to a possible role of horizontal gene transfer in the evolutionary history of these lineages. 相似文献
97.
Timothy N Storms Victoria L Clyde Linda Munson Edward C Ramsay 《Journal of zoo and wildlife medicine》2003,34(3):231-238
Blastomycosis was diagnosed in six nondomestic felids from eastern Tennessee, including two Asian lions (Panthera leo persicus), one African lion (Panthera leo), one Siberian tiger (Panthera tigris), one cheetah (Acinonyx jubatus), and one snow leopard (Panthera uncia). Clinical signs included lethargy, anorexia, weight loss, dyspnea, sneezing. ataxia, and paresis. Variable nonspecific changes included leukocytosis, monocytosis, moderate left shift of neutrophils, moderate hypercalcemia, hyperproteinemia, and hyperglobulinemia. Thoracic radiographs revealed interstitial and alveolar changes, consolidation or collapse of a lung lobe, bullae formation, and a pulmonary mass. Agar gel immunodiffusion (AGID) serology for Blastomyces dermatitidis was performed in five felids and was positive in three. The tiger had cerebral blastomycosis and was positive for AGID serologic tests of both cerebrospinal fluid and serum. One percutaneous lung aspirate in the snow leopard and one bronchial aspirate in an Asian lion demonstrated B. dermatitidis organisms. whereas tracheal wash samples and a nasal discharge were nondiagnostic in others. Treatment with itraconazole was attempted in four cats. The tiger improved before euthanasia, whereas the others did not survive beyond initial treatments. In four felids, B. dermatitidis was found in the lungs and tracheobronchial lymph nodes associated with a florid pyogranulomatous reaction; the tiger had a pyogranulomatous encephalomyelitis, and the cheetah had a single pulmonary granuloma. Thoracic radiography, cytologic examination of lung lesion aspirates, and B. dermatitidis AGID serology should be performed on clinically ill zoo felids in endemic areas to rule out blastomycosis. 相似文献
98.
Tang C Lennon EM Fredrickson GH Kramer EJ Hawker CJ 《Science (New York, N.Y.)》2008,322(5900):429-432
The manufacture of smaller, faster, more efficient microelectronic components is a major scientific and technological challenge, driven in part by a constant need for smaller lithographically defined features and patterns. Traditional self-assembling approaches based on block copolymer lithography spontaneously yield nanometer-sized hexagonal structures, but these features are not consistent with the industry-standard rectilinear coordinate system. We present a modular and hierarchical self-assembly strategy, combining supramolecular assembly of hydrogen-bonding units with controlled phase separation of diblock copolymers, for the generation of nanoscale square patterns. These square arrays will enable simplified addressability and circuit interconnection in integrated circuit manufacturing and nanotechnology. 相似文献
99.
Aulanier G Golub L Deluca EE Cirtain JW Kano R Lundquist LL Narukage N Sakao T Weber MA 《Science (New York, N.Y.)》2007,318(5856):1588-1591
Magnetic reconnection of solar coronal loops is the main process that causes solar flares and possibly coronal heating. In the standard model, magnetic field lines break and reconnect instantaneously at places where the field mapping is discontinuous. However, another mode may operate where the magnetic field mapping is continuous but shows steep gradients: The field lines may slip across each other. Soft x-ray observations of fast bidirectional motions of coronal loops, observed by the Hinode spacecraft, support the existence of this slipping magnetic reconnection regime in the Sun's corona. This basic process should be considered when interpreting reconnection, both on the Sun and in laboratory-based plasma experiments. 相似文献
100.
Small-scale heterogeneity in carbon dioxide, nitrous oxide and methane production from aggregates of a cultivated sandy-loam soil 总被引:2,自引:0,他引:2
Benjamin K. Sey Ameur M. Manceur Joann K. Whalen Edward G. Gregorich Philippe Rochette 《Soil biology & biochemistry》2008,40(9):2468
Spatial variability in carbon dioxide (CO2), nitrous oxide (N2O) and methane (CH4) emissions from soil is related to the distribution of microsites where these gases are produced. Porous soil aggregates may possess aerobic and anaerobic microsites, depending on the water content of pores. The purpose of this study was to determine how production of CO2, N2O and CH4 was affected by aggregate size and soil water content. An air-dry sandy loam soil was sieved to generate three aggregate fractions (<0.25 mm, 0.25–2 mm and 2–6 mm) and bulk soil (<2 mm). Aggregate fractions and bulk soil were moistened (60% water-filled pore space, WFPS) and pre-incubated to restore microbial activity, then gradually dried or moistened to 20%, 40%, 60% or 80% WFPS and incubated at 25 °C for 48 h. Soil respiration peaked at 40% WFPS, presumably because this was the optimum level for heterotrophic microorganisms, and at 80% WFPS, which corresponded to the peak N2O production. More CO2 was produced by microaggregates (<0.25 mm) than macroaggregate (>0.25 mm) fractions. Incubation of aggregate fractions and soil at 80% WFPS with acetylene (10 Pa and 10 kPa) and without acetylene showed that denitrification was responsible for 95% of N2O production from microaggregates, while nitrification accounted for 97–99% of the N2O produced by macroaggregates and bulk soil. This suggests that oxygen (O2) diffusion into and around microaggregates was constrained, whereas macroaggregates remained aerobic at 80% WFPS. Methane consumption and production were measured in aggregates, reaching 1.1–6.4 ng CH4–C kg−1 soil h−1 as aggregate fractions and soil became wetter. For the sandy-loam soil studied, we conclude that nitrification in aerobic microsites contributed importantly to total N2O production, even when the soil water content permitted denitrification and CH4 production in anaerobic microsites. The relevance of these findings to microbial processes controlling N2O production at the field scale remains to be confirmed. 相似文献