Ursache des Triebsterbens an Heidelbeeren in Norddeutschland: Schadpilze oder Winterfrost?

In the summer of 2010 and during the following two seasons, a twig dieback of blueberries (Vaccinium corymbosum) was observed in southern Lower Saxony especially in younger plantations of the variety ‘Duke’. The sudden onset of a chlorosis and necrosis of individual twigs was associated with a destruction of the vascular cambium and with longitudinal streaks of brown discolourations in the xylem tissue deposited during the previous year. New shoots were often formed from the intact roots and from regions of older twigs located immediately above the soil surface. Potentially pathogenic fungi, including Godronia cassandrae, Phomopsis spp., Diplodia seriata, Pestalotiopsis sp., Paraconiothyrium sporulosum, Colletotrichum acutatum, Fusarium avenaceum and F. lateritium, were found sporadically on dead twigs, each fungus being present in 6 or fewer of the 12 plantations surveyed. Winter frost damage of xylem tissues was determined to be the main cause of the disease, facilitated by a delayed termination of vegetative growth which led to a lack of winter hardiness at the time-point of the first serious frost events.     

Estimation of transmission parameters of a fluoroquinolone-resistant Escherichia coli strain between pigs in experimental conditions

Antimicrobial resistance is of primary importance regarding public and animal health issues. Persistence and spread of resistant strains within a population contribute to the maintenance of a reservoir and lead to treatment failure. An experimental trial was carried out to study the horizontal transmission of a fluoroquinolone-resistant Escherichia coli strain from inoculated to naïve pigs. All naïve contact pigs had positive counts of fluoroquinolone-resistant E. coli after only two days of contact. Moreover, re-infections of inoculated pigs caused by newly contaminated animals were suspected. A maximum likelihood method, based on a susceptible-infectious-susceptible (SIS) model, was used to determine the transmission parameters. Two transmission levels were identified depending on the quantity of bacteria shed by infected individuals: (i) low-shedders with bacterial counts of resistant E. coli in the faeces between 5*10³ and 10⁶ CFU/g (β_L = 0.41 [0.27; 0.62]), (ii) high shedders with bacterial counts above 10⁶ CFU/g (β_H = 0.98 [0.59; 1.62]). Hence, transmission between animals could be pivotal in explaining the persistence of resistant bacteria within pig herds.     

Infection conditions for Neofabraea perennans and Phacidiopycnis washingtonensis on developing apple fruit in the orchard

European Journal of Plant Pathology - In Northern Germany, a major share of postharvest losses of apple fruit is due to preharvest infections by pathogenic fungi. Little is known about their...     

A rapid and simple method for determining fungicide resistance in Botrytis

A simple test based on the germination of conidia of Botrytis on agar media augmented with various fungicides has been developed. Average concentrations causing a 50% reduction of germ-tube growth (EC₅₀) of highly sensitive isolates were determined on 1% malt extract agar (thiophanate-methyl 0.090 ppm; iprodione 0.566 ppm; fludioxonil 0.026 ppm; fenhexamid 0.144 ppm), 1% malt extract agar with 100 ppm salicyl hydroxamic acid (QoI fungicides, viz. trifloxystrobin 0.009 ppm; pyraclostrobin 0.013 ppm; azoxystrobin 0.087 ppm), 0.5% yeast extract agar (boscalid 0.069 ppm) and 0.5% sucrose agar (cyprodinil 0.053 ppm). In order to detect different levels of resistance against these various fungicides, two discriminatory concentrations were identified for each compound. A routine assay method was developed in which drops of a conidial suspension harvested directly from diseased plant material or sporulating cultures were incubated on each of 20 different agar media. Because of a very short time-span of 24–48 h between sample collection and evaluation of results, field-specific information on the occurrence, frequency and types of resistance of Botrytis against common botryticides in soft-fruit production may be generated prior to the main fungicide spray season at blossom time.

     

Are microconidia infectious principles in Neonectria ditissima?

Journal of Plant Diseases and Protection - Comparative studies of different isolates of Neonectria ditissima obtained from canker lesions and rotten fruit showed that both five-septate macroconidia...     

Extent and Management of Low pH Soils in Ghana

A review of soil acidity problems with regards to causes, extent and management in Ghana shows that the extent of soil acidity has changed within a period of 2–3 decades. In the 1970s' soil acidity was a major problem in the Western region and the lowlands. Oxisols and Ultisols are common soils found in this area. However, anthropogenic causes have augmented natural causes resulting in more low pH soils. Soil acidity has therefore become a serious problem throughout the country. Anthropogenic causes identified include general agronomic practices such as tillage, use of mineral fertilizers and continuous cultivation. Rates of 1–2 t ha⁻¹ lime on ultisols and oxisols in Ghana are more than adequate to meet the calcium demands and sufficient to neutralize residual acidity of any nitrogen fertilizer. Addition of organic amendments on low pH soils was also found to be beneficial and resulted in increased yields. The adoption of the "sawah" technology for lowland rice production has also resulted in improved water management, increased nutrient availability and significant increases in rice yields.     

Two-Year Weed Seedling Population Responses to a Post-Emergent Method of Site-Specific Weed Management

Field experiments were conducted to determine how a site-specific weed management practice in Zea mays L. influenced the numerical and spatial distribution of a naturally occurring weed infestation in Z. mays and the succeeding Beta vulgaris L. crop. Compared to conventional broadcast herbicide applications, site-specific herbicide applications reduced herbicide load by 11.5 and 98.0% in two separate Z. mays fields. The broad range in outcomes was attributed to the spatial aggregation and density of target weed populations. While herbicide use was successfully reduced at field locations with low weed density, most survivors of multiple control tactics were in locations with the highest initial density. A greater understanding of interactions between weed management and weed density would increase the likelihood that site-specific weed management offers long-term improvements over conventional approaches.     

Metabolite profiling identifies a key role for glycine in rapid cancer cell proliferation

Metabolic reprogramming has been proposed to be a hallmark of cancer, yet a systematic characterization of the metabolic pathways active in transformed cells is currently lacking. Using mass spectrometry, we measured the consumption and release (CORE) profiles of 219 metabolites from media across the NCI-60 cancer cell lines, and integrated these data with a preexisting atlas of gene expression. This analysis identified glycine consumption and expression of the mitochondrial glycine biosynthetic pathway as strongly correlated with rates of proliferation across cancer cells. Antagonizing glycine uptake and its mitochondrial biosynthesis preferentially impaired rapidly proliferating cells. Moreover, higher expression of this pathway was associated with greater mortality in breast cancer patients. Increased reliance on glycine may represent a metabolic vulnerability for selectively targeting rapid cancer cell proliferation.