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31.
True-to-type clonal fidelity is one of the most important pre-requisites in micropropagation of crop species. Genetic fidelity of in vitro raised 45 plants of gerbera (Gerbera jamesonii Bolus) derived from three different explants, viz., capitulum, leaf and shoot tips, was assessed by 32 ISSR markers, for their genetic stability. Out of 32 ISSR markers, 15 markers produced clear, distinct and scorable bands with an average of 5.47 bands per marker. The markers designed from AG motif amplified more number of bands. The markers anchored at 3′ ends produced high number of consistent bands than unanchored markers. Fifteen ISSR markers generated a total of 3773 bands, out of which 3770 were monomorphic among all the clones. The Jaccard's similarity coefficient revealed that out of 45 clones derived from different explants, 44 were grouped into a single large cluster alongwith the mother plant with a similarity coefficient value of 1.00, whereas one clone (C38) remained ungrouped. The clones derived from capitulum and shoot tip explants did not show any genetic variation, whereas, one of the leaf-derived clones exhibited some degree of variation. 相似文献
32.
Abdulaziz Anas Rosamma Philip & Isaac Sarojeni Bright Singh 《Aquaculture Research》2008,39(8):885-890
Chitosan has been widely accepted as a wall material for preparing microcapsules of various purposes in human medicine. The possibility of using chitosan as a wall material for microencapsulating nutrients and drugs for aquaculture purposes, specifically to Macrobrachium rosenbergii larvae was evaluated in this study. Two types of chitosan-coated microcapsules were prepared using either acetone (MEC-A) or NaOH (MEC-N) as the cross-linking agents. They were compared with a microbound diet relative to total leaching of nutrients and free amino acids (FAA). Among the microcapsules, MEC-N showed the lowest level of total leaching of nutrients (23.3%) during 5 h of immersion in seawater and released 65% FAA after 60 min. During laboratory trials, 75% larvae had accepted the MEC-N capsule. The results of the study suggest that chitosan can be used as a wall material for preparing microcapsules to deliver drugs and nutrients to M. rosenbergii larvae. 相似文献
33.
In the present study, water and nitrogen interaction on soil profile water extraction and evapo-transpiration (ET) was investigated taking a field experiment on a clay loam soil (Typic Haplustept) at the Indian Agricultural Research Institute, New Delhi with four consecutive crops (maize-wheat-maize-wheat) taken from July 2002 to April 2004. Three levels of water regime, namely W1, W2 and W3 referring to limited, medium and maximum irrigation were applied to each crop depending on the seasonal rainfall and the critical crop growth stage. The three water regimes were used with five nitrogen levels from T1 to T5, (T1, 0% N; T2, 75% N; T3, 100% N; T4, 150% N; T5, 100% N from organic source) in a split plot design for the four crops grown in sequence.Significant water and nitrogen interaction was observed for ET and soil profile water extraction pattern. Averaged across nitrogen treatments, ET in W2 and W3 were higher by 17 and 26%, respectively than W1 in maize 2002 and by 12 and 19% in maize 2003. In case of wheat, ET in W2 and W3 were higher by 27 and 58% than W1 in 1st crop and by 37 and 70% in 2nd crop. The effect of nitrogen regime, however, was prominent in both crops of maize and wheat, with significantly higher profile soil moisture depletion in T4 of each water regime. In all cases, lowest water depletion was observed in control plots receiving 0% N.In both crops, water extraction from surface 60 cm was highest in W3 followed by W2 and W1. In maize, the % extraction from 0 to 60 cm layer varied from 71 to 76% (W1), 70-79% (W2) and 75-82% (W3), whereas the values for wheat were 70-77, 72-79 and 75-83% for W1, W2 and W3, respectively. The 90-120 cm layer contributed only 3-14% to total water extraction in both the crops. From 90 to 120 cm layer, higher extraction was observed in W1 as compared to W3. The extraction values in W1, W2 and W3 in maize were 9-13, 7-14 and 3-9, respectively, whereas the corresponding values in wheat were 8-14, 5-12 and 3-7% for the three water regimes. Effect of nitrogen treatments on water extraction from deeper layer was observed with higher extraction in highest fertilized treatment (T4) as compared to other treatments. 相似文献
34.
S.K. Jalota K.B. Singh R.K. Gupta Anil Sood S. Panigrahy 《Agricultural Water Management》2009,96(7):1096-1104
Individual effect of different field scale management interventions for water saving in rice viz. changing date of transplanting, cultivar and irrigation schedule on yield, water saving and water productivity is well documented in the literature. However, little is known about their integrated effect. To study that, field experimentation and modeling approach was used. Field experiments were conducted for 2 years (2006 and 2007) at Punjab Agricultural University Farm, Ludhiana on a deep alluvial loamy sand Typic Ustipsamment soils developed under hyper-thermic regime. Treatments included three dates of transplanting (25 May, 10 June and 25 June), two cultivars (PR 118 inbred and RH 257 hybrid) and two irrigation schedules (2-days drainage period and at soil water suction of 16 kPa). The model used was CropSyst, which has already been calibrated for growth (periodic biomass and LAI) of rice and soil water content in two independent experiments. The main findings of the field and simulation studies conducted are compared to any individual, integrated management of transplanting date, cultivar and irrigation, sustained yield (6.3-7.5 t ha−1) and saved substantial amount of water in rice. For example, with two management interventions, i.e. shifting of transplanting date to lower evaporative demand (from 5 May to 25 June) concomitant with growing of short duration hybrid variety (90 days from transplanting to harvest), the total real water saving (wet saving) through reduction in evapotranspiration (ET) was 140 mm, which was almost double than managing the single, i.e. 66 mm by shifting transplanting or 71 mm by growing short duration hybrid variety. Shifting the transplanting date saved water through reduction in soil water evaporation component while growing of short duration variety through reduction in both evaporation and transpiration components of water balance. Managing irrigation water schedule based on soil water suction of 16 kPa at 15-20 cm soil depth, compared to 2-day drainage, did not save water in real (wet saving), however, it resulted into apparent water saving (dry saving). The real crop water productivity (marketable yield/ET) was more by 17% in 25th June transplanted rice than 25th May, 23% in short duration variety than long and 2% in irrigation treatment of 16 kPa soil water suction than 2-days drainage. The corresponding values for the apparent crop water productivity (marketable yield/irrigation water applied) were 16, 20 and 50%, respectively. Pooled experimental data of 2 years showed that with managing irrigation scheduling based on soil water suction of 16 kPa at 15-20 cm soil depth, though 700 mm irrigation water was saved but the associated yield was reduced by 277 kg ha−1. 相似文献
35.
Expected yield losses as a function of quality and quantity of water applied for irrigation are required to formulate guidelines for the effective utilisation of marginal quality waters. In an experiment conducted during 2004-2006, double-line source sprinklers were used to determine the separate and interactive effects of saline and alkali irrigation waters on wheat (Triticum aestivum L.). The study included three water qualities: groundwater (GW; electrical conductivity of water, ECw 3.5 dS m−1; sodium adsorption ratio, SAR 9.8 mmol L−1; residual sodium carbonate, RSC, nil) available at the site, and two synthesized waters, saline (SW; ECw 9.4 dS m−1, SAR 10.3 mmol L−1; RSC nil) and alkali (AW; ECw 3.7 dS m−1, SAR 15.1 mmol L−1; RSC 9.6 meq. L−1). The depths of applied SW, AW, and GW per irrigation ranged from 0.7 to 3.5 cm; the depths of applied mixtures of GW with either SW (MSW) or AW (MAW) ranged from 3.2 to 5 cm. Thereby, the water applied for post-plant irrigations using either of GW, SW or AW ranged between 15.2 and 34.6 cm and 17.1 and 48.1 cm during 2004-2005 and 2005-2006, respectively and the range was 32.1-37.0 and 53.1-60.0 cm for MSW or MAW. Grain yields, when averaged for two years, ranged between 3.08 and 4.36 Mg ha−1, 2.57 and 3.70 Mg ha−1 and 2.73 and 3.74 Mg ha−1 with various quantities of water applied using GW, SW and AW, respectively, and between 3.47 and 3.75 Mg ha−1 and 3.63 and 3.77 Mg ha−1 for MSW and MAW, respectively. The water production functions developed for the two sets of water quality treatments could be represented as: RY = 0.528 + 0.843(WA/OPE) − 0.359(WA/OPE)2 − 0.027ECw + 0.44 × 10−2(WA/OPE) × ECw for SW (R2 = 0.63); RY = 0.446 + 0.816(OPE/WA) − 0.326(WA/OPE)2 − 0.0124RSC − 0.55 × 10−4(WA/OPE) × RSC for AW (R2 = 0.56). Here, RY, WA and OPE are the relative yields in reference to the maximum yield obtained with GW, water applied for pre- and post-plant irrigations (cm), and open pan evaporation, respectively. Crop yield increased with increasing amount of applied water for all of the irrigation waters but the maximum yields as obtained with GW, could not be attained even with increased quantities of SW and AW. Increased frequency of irrigation with sprinklers reduced the rate of yield decline with increasing salinity in irrigation water. The sodium contents of plants increased with salinity/alkalinity of sprinkled waters as also with their quantities. Simultaneous decrease in potassium contents resulted in remarkable increase in Na:K ratio. 相似文献
36.
Tor tor is an important game and food fish of India with a distribution throughout Asia from the trans-Himalayan region to the Mekong River basin to Malaysia, Pakistan, Bangladesh and Indonesia. A new cell line named TTCF was developed from the caudal fin of T. tor for the first time. The cell line was optimally maintained at 28°C in Leibovitz-15 (L-15) medium supplemented with 20% fetal bovine serum (FBS). The propagation of TTCF cells showed a high plating efficiency of 63.00%. The cytogenetic analysis revealed a diploid count of 100 chromosomes at passage 15, 30, 45 and 60 passages. The viability of the TTCF cell line was found to be 72% after 6 months of cryopreservation in liquid nitrogen (-196°C). The origin of the cell lines was confirmed by the amplification of 578- and 655-bp sequences of 16S rRNA and cytochrome oxidase subunit I (COI) genes of mitochondrial DNA (mtDNA) respectively. TTCF cells were successfully transfected with green fluorescent protein (GFP) reporter plasmids. Further, immunocytochemistry studies confirm its fibroblastic morphology of cells. Genotoxicity assessment of H?O? in TTCF cell line revealed the utility of TTCF cell line as in vitro model for aquatic toxicological studies. 相似文献
37.
Subham Mukherjee Jagdeep Singh Bhupinder Pal Singh 《Archives of Agronomy and Soil Science》2020,66(6):778-791
ABSTRACTThermo-chemical conversion of crop residues to produce biochar is an emerging strategy in the context of sustainable phosphorous (P) use and residue management. An incubation study for 90 d was conducted to investigate the effects of rice-residue biochar (0, 10, 20 and 40 g kg?1) in combination with inorganic-P (KH2PO4) (0, 25 and 50 mg kg?1) on phosphorous availability in medium- and high-P status soils. Increasing biochar addition rates alone or in combination with inorganic-P resulted in a significant increase in P pools, i.e. plant available P or Olsen-P (from 8 to 132 mg kg?1 in medium-P and 15 to 160 mg kg?1 in high-P soils), microbial biomass P and various mineral-bound inorganic-P fractions in the order (Ca-P > organic-P > Al-P > loosely held/soluble-P > Fe-P > reductant soluble-P). Further, lower phosphatase activity (19–50%) with increasing rates of biochar addition in both soils elucidates the ability of biochar to act as a long-term source of available P in the experimental soils. The results demonstrate that rice-residue biochar can directly or indirectly enhance the status of available P in soils and hence can be used as a beneficial amendment to meet the crop P demand. 相似文献
38.
Shiveshwar Pratap Singh Room Singh Mukesh Prasad Singh Vijay Prasad Singh 《Journal of plant nutrition》2014,37(4):618-632
A field experiment was conducted at farmer's field in Mollisols of Tarai soils in Uttarakhand (India), to assess the direct and residual effect of sulfur fertilization in wheat-soybean cropping sequence. Four levels of sulfur (S; 0, 15, 30, and 45 kg ha?1) were applied to main crop (wheat) along with recommended dose of nitrogen (N), phosphorous (P), and potassium (K). The direct and residual effect of sulfur at highest level showed 27 and 6 percent increase in grain yield of wheat and soybean over control, respectively. The increase in grain yield of wheat was significant at each sulfur level. The direct as well as residual effect of sulfur showed significant increase in sulfur concentration and its uptake by grain and straw except increase in sulfur concentration and uptake by soybean straw. In wheat-soybean cropping sequence, the agronomic efficiency and apparent sulfur recovery decreased with increase in levels of sulfur, but the percent response increased with increasing sulfur application. Different forms of sulfur such as total sulfur, organic sulfur, calcium chloride extractable sulfur, potassium dihydrogen phosphate extractable sulfur, and non-sulfate sulfur in post-harvest soil increased according to sulfur level applied but it decreased under control and also after residual crops. The buildup of sulfur in surface soils was greater than in the deeper soils. Application of sulfur showed the positive sulfur balance and it increased with increase in sulfur level, while it was negative under control. A major portion (46–62%) of applied sulfur contributed to increase in sulfur content of root zone soil followed by unaccounted component (25–40%) and small portion (11–18%) was absorbed by wheat plant as uptake. 相似文献
39.
Commercial grade zinc (Zn) sulfate hepta hydrate (ZnSHH) is the most widely used source of Zn in India and several other countries for amelioration of Zn deficiency in crops. However, it releases water of hydration at temperature above 30°C and forms lumps on storage, which make it difficult to handle it and apply in fields. Therefore, conditioning of ZnSHH with ZnO and neem oil reduces the release of water of hydration and prevents lumps formation and can be well stored. Field experiments were conducted at the research farm of the Indian Agricultural Research Institute, New Delhi, India during rice growing seasons (July-November) of 2009 and 2010 to study the effect of conditioning ZnSHH with ZnO and neem oil on growth, productivity and Zn fortification of rice (Oryza sativa) grain and uptake by Basmati rice ‘Pusa 1121’. The experiment was conducted in a randomized block design with 3 replications comprised of 9 treatments of Zn fertilization. The present study shows that when conditioned with 2% ZnO and 4% neem oil ZnSHH improved yield attributes, grain and straw yields, Zn uptake and partial factor productivity (PFP), agronomic efficiency (AE), recovery efficiency (RE), and physiological efficiency (PE) of Zn in Basmati rice ‘Pusa 1121’. In general, ZnO was inferior to ZnSHH. Application of ZnSHH conditioned with 2% ZnO and 4% neem oil can be a better source of Zn for transplanted puddled Basmati rice on Zn deficient soils. 相似文献
40.
Suitability of mapped sequence tagged microsatellite site markers for establishing distinctness, uniformity and stability in aromatic rice 总被引:6,自引:0,他引:6
R.K. Singh R.K. Sharma A.K. Singh V.P. Singh N.K. Singh S.P. Tiwari T. Mohapatra 《Euphytica》2004,135(2):135-143
At present, testing for distinctness, uniformity and stability (DUS) of crop varieties relies on a set of morphological characters.
These characters suffer fromthe limitations of number, interaction with the environment in which the variety grows and subjectivity
in decision-making. The potential of DNA-based markers such as sequence tagged microsatellite site (STMS), for establishing
DUS merits investigation. In the present study, a set of 55 mapped STMS markers, selected from 12 linkage groups of rice genome,
was used to examine distinctness of 23 aromatic rice genotypes including the commercially important Basmati varieties. Forty-one
of these markers (74.5%) showed polymorphism between the varieties. The number of alleles per locus ranged from 2–4 with an
average of 2.3. The polymorphism information content (PIC) of the markers varied from 0.083 to 0.665 with an average of 0.338.
All the varieties could be differentiated from each other at a low probability (0.07×10-13) of identical match by chance. The marker-based clustering of the varieties corresponded with the known phenotypic classification,
thereby providing confidence in the distinctness established by the mapped STMS markers. The utility of these markers to study
uniformity and stability was analysed using a commercially important crossbred Basmati rice variety Pusa Basmati 1(IET-10364)
that contributes about 40–50% of Basmati rice export from India. Genotyping of twenty individual plants, grown from the nucleus,
breeder, foundation, certified and farmer's saved seed samples using all the 55 markers revealed no variation among the plants.
These observations suggested that the set of mapped markers employed in this study could be further used for establishing
distinctness of aromatic rice varieties and for studying DUS of the important commercial variety Pusa Basmati 1.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献