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181.
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Junia Vianna Corrêa da Silva Fernando César Juliatti João Renato Vaz da Silva Fernanda Carvalho Barros 《European journal of plant pathology / European Foundation for Plant Pathology》2011,131(3):409-418
Soybean rust is caused by an obligate parasite (Phakopsora pachyrhizi) which has spread in Brazil in each new season since 2001 and, despite the efforts to control the disease, losses have occurred
every year. Its control demands several tactics amongst which chemical control with fungicides is the main method and remains
indispensable. Control strategies such as the use of cultivars with partial resistance are desirable, but are not yet commercially
available. The present study analyzed the existing differences in the reactions of short, medium and long cycle soybean cultivars
against Asian rust and their responses to fungicide sprays. The experiment was conducted at Uberlandia-MG, Brazil, under field
conditions from December 2007 to May 2008, in the Syngenta Seeds Experimental Station. The high pressure of the disease in
the experiment simulated the natural pressure that the disease often reaches in Brazil. The studied variables were: visual
severity (percentage of infected leaf area), percentage defoliation and productivity (kg ha−1). Disease severity was expressed as AUDPC (area under disease progress curve). Variance analysis and comparison of means
by the Tukey test (5% significance) were done for all variables studied. Significant differences were observed between cultivar
effects and chemical control programs. The results obtained here indicate that the cultivars M-Soy 8199RR and Emgopa 315RR
were less susceptible to disease, and that a control program termed “monitoring” (in which the appearance of new pustules
of the pathogen were monitored to make the decision at each fungicide spray) was the most effective. 相似文献
183.
Yong Zhang Yu Chen Xiaofeng Zhu Ying Xu Yiping Hou Tongchun Gao Mingguo Zhou 《Phytoparasitica》2011,39(4):393-401
Xanthomonas oryzae pv. oryzicola, the causal agent of rice leaf streak disease, was found to be sensitive to streptomycin (an aminocyclitol glycoside antibiotic),
by inhibition of protein synthesis resulting from interference with translational proofreading. This study aimed to determine
the molecular resistance mechanism of X. oryzae pv. oryzicola to streptomycin. Seven streptomycin-resistant mutants were obtained by UV induction or streptomycin selection. These mutants
can grow at 100 μg ml−1 of streptomycin while the wild-type strain (RS105) cannot grow at 5 μg ml−1. Sequencing indicated that the rpsL gene encoding ribosomal protein S12 has 375 bp encoding 125 amino acid residues. In all resistant strains, a mutation in
which AAG was substituted for AGG (Lys→Arg) occurred either at codon 43 or 88. Two plasmids, pUFRRS and pUFRRX, were constructed
by ligating the rpsL gene into the cosmid pUFR034. The plasmids pUFRRS and pUFRRX containing the Lys→Arg mutation of the rpsL gene conferred streptomycin resistance to the sensitive wild-type strain by electroporation. Both transformants, RS1 and
RS2, could grow in the medium containing 50 μg ml−1 of streptomycin. A mutation at codon 43 or 88 in rpsL can result in resistance of Xanthomonas oryzae pv. oryzicola to streptomycin. 相似文献
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Gianluca Neglia Bianca Gasparrini Domenico Vecchio Marcello Rubessa Rossella Di Palo Luigi Zicarelli Giuseppe Campanile 《Tropical animal health and production》2010,42(6):1243-1247
The aim of this study was to evaluate the effect of exogenous progesterone supplementation on superovulatory response in buffaloes
that has undergone a multiple ovulation program. Fourteen Mediterranean buffaloes were divided into two groups and received
a 4-day decreasing dosage of an equal mixture of 500 IU of FSH and LH starting on day 8 of the cycle. In group A (n = 7) a progesterone-releasing intravaginal device was removed on day 8, whereas in group B (n = 7) it was left till day 10, when PGF2a {\hbox{PG}}{{\hbox{F}}_{{2}\alpha }} was administered. Eighty hours later, buffaloes were artificially inseminated and after 6 days they undergone uterine flushing.
A higher (P < 0.05) number of corpora lutea (8.3 vs. 5.7) and embryo/flushing/buffalo (2.3 vs. 1.3) were recorded in group B vs. group
A if responsive buffaloes are considered (n = 12) and the number of corpora lutea was highly correlated with the number of embryos (r = 0.65; P < 0.05). In conclusion, progesterone supplementation during the first 2 days of the superovulation treatment seems to enhance
the recovery rate in buffalo species. A high ovulation rate, associated with a high number of corpora lutea, can represent
a parameter for estimating embryo recovery. 相似文献
187.
Krishna Mohan Vijay Kumar Mihir Sarkar B. S. Prakash 《Tropical animal health and production》2010,42(1):21-26
The objective of this study were (1) to establish the duration of behavioral estrus signs and timing of ovulation in Murrah
buffaloes (n = 10) and (2) to determine relationship between behavioral estrus signs with change in plasma estrogen concentrations.
Estrus and its behavioral signs were detected at hourly intervals by visual observations, per recta examination of genitalia
and bull parading four times in a day for 30 minutes each. Among the behavioral signs of estrus, swollen vulva (80%) was the
best indicator of estrus followed by excitement (70%). Among the duration of behavioral estrus signs the first and longest
duration of estrus signs was swollen vulva which was seen upto 19.8 ± 0.8 h after onset of estrus. The mean total duration
of estrus symptoms from appearance to disappearance of all the behavioral estrus symptoms was 23.5 ± 1.7 h. All the behavioral
estrus symptoms were observed during the period of estrogen surge. Endocrine profile during the periestrus period showed that
the mean peak concentrations of total estrogen 23.9 ± 3.9 pg/ml occurred at 8.8 ± 1.7 h after onset of estrus. The average
number of estrus symptoms observed per animal during onset of spontaneous estrus was 5.7. Ovulation occurred after 37.4 ± 1.7 h
after onset of estrus and 13.4 ± 1.0 h after end of total estrogen surge respectively. In conclusion, our results suggest
that all signs of behavioral estrus occurred during the preovulatory rise in estrogens. The first sign of estrus to be observed
was a swollen vulva and this symptom persisted the longest. 相似文献
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