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111.
Huntley JF van den Broek A Machell J Mackellar A Pettit D Meikle L Barcham G Meeusen EN Smith D 《Veterinary parasitology》2005,127(3-4):323-332
The present study confirms that following infection with the ectoparasitic sheep scab mite, Psoroptes ovis, there is a rapid (within 24 h) inflammatory influx of eosinophils and apoptosis of the keratinocytes at the site of infection. In order to investigate whether these inflammatory reactions are important in the maintenance of mite infection, a group of animals were treated daily after the establishment of infection with the potent anti-inflammatory drug, Cyclosporin A. The course of infection was monitored by determining the lesion area and mite numbers, systemic antibody and blood eosinophils, as well as the inflammatory cells and T cell sub-populations within the lesion throughout the 6-week duration of the experiment. These parameters were compared with those in a similar infected control (non-treated) group. In control infected animals, the lesion area and mite numbers increased steadily throughout the 6-week period. In contrast, lesion area and mite numbers were severely depressed in the group which received Cyclosporin A. Local and systemic eosinophils, and systemic antibody were also significantly reduced in the drug treated animals, compared to controls. Surprisingly however, the number of lesional pan T cells, T helper cells, gammadeltaT cells and dendritic cells in Cyclosporin A treated animals were either the same, or significantly (P < 0.05) enhanced when compared to the control infected animals at the termination of the experiment. The results will be discussed in terms of the role of the dermal inflammatory response in the establishment and maintenance of the sheep scab mite. 相似文献
112.
Kreuder C Miller MA Lowenstine LJ Conrad PA Carpenter TE Jessup DA Mazet JA 《American journal of veterinary research》2005,66(2):289-299
OBJECTIVE: To describe cardiac lesions and identify risk factors associated with myocarditis and dilated cardiomyopathy (DCM) in beach-cast southern sea otters. ANIMALS: Free-ranging southern sea otters. PROCEDURE: Sea otters were necropsied at the Marine Wildlife Veterinary Care and Research Center from 1998 through 2001. Microscopic and gross necropsy findings were used to classify sea otters as myocarditis or DCM case otters or control otters. Univariate, multivariate, and spatial analytical techniques were used to evaluate associations among myocarditis; DCM; common sea otter pathogens; and potential infectious, toxic, and nutritional causes. RESULTS: Clusters of sea otters with myocarditis and DCM were identified in the southern aspect of the sea otter range from May to November 2000. Risk factors for myocarditis included age, good body condition, and exposure to domoic acid and Sarcocystis neurona. Myocarditis associated with domoic acid occurred predominantly in the southern part of the range, whereas myocarditis associated with S. neurona occurred in the northern part of the range. Age and suspected previous exposure to domoic acid were identified as major risk factors for DCM. A sample of otters with DCM had significantly lower concentrations of myocardial L-carnitine than control and myocarditis case otters. CONCLUSIONS AND CLINICAL RELEVANCE: Cardiac disease is an important cause of death in southern sea otters. Domoic acid toxicosis and infection with S. neurona are likely to be 2 important causes of myocarditis in sea otters. Domoic acid-induced myocarditis appears to progress to DCM, and depletion of myocardial L-carnitine may play a key role in this pathogenesis. 相似文献
113.
Coetzee JF Gehring R Tarus-Sang J Anderson DE 《Veterinary anaesthesia and analgesia》2010,37(6):566-578
ObjectiveTo describe the pharmacokinetics, cortisol response and behavioral changes associated with administration of sub-anesthetic xylazine and ketamine prior to castration.Study designProspective, randomized experiment.AnimalsTwenty-two male beef calves (260-310 kg).MethodsCalves were randomly assigned to receive the following treatment immediately prior to surgical or simulated castration; 1) uncastrated, placebo-treated control (CONT) (n = 4), 2) Castrated, placebo treated control (CAST) (n = 6), 3) castrated with intravenous xylazine (X) (0.05 mg kg?1) (n = 6), and 4) castrated with IV xylazine (X) (0.05 mg kg?1) combined with ketamine (K) (0.1 mg kg?1) (n = 6). Blood samples collected over 10 hours post-castration were analyzed by LC-MS-MS for drug concentrations and chemiluminescent immunoassay for cortisol determination.ResultsDrug concentrations during the first 60 minutes post-castration fit a one-compartment open model with first-order elimination. The harmonic mean elimination half-lives (± pseudo SD) for X, X with K and K were 12.9 ± 1.2, 11.2 ± 3.1 and 10.6 ± 2.8 minutes, respectively. The proportion of the total area under the effect curve (AUEC) for cortisol during this period was significantly lower in the X group (13 ± 3%; p = 0.006) and the X+K group (14 ± 2%; p = 0.016) compared with the CAST calves (21 ± 2%). However, after 300 minutes the AUEC in the X group was higher than CAST. Significantly more calves demonstrated attitude that was unchanged from pre-manipulation behavior in the CONT (p = 0.021) and X+K treated calves (p = 0.0051) compared with the CAST calves.ConclusionsBehavioral changes and lower serum cortisol concentrations during the first 60 minutes post-castration were associated with quantifiable xylazine and ketamine concentrations.Clinical relevanceLow doses of xylazine and ketamine administered immediately prior to castration may offer a safe, efficacious and cost-effective systemically administered alternative or adjunct to local anesthesia. 相似文献
114.
Peter L. M. Msoffe David Bunn A. P. Muhairwa M. M. A. Mtambo H. Mwamhehe A. Msago M. R. S. Mlozi Carol J. Cardona 《Tropical animal health and production》2010,42(2):253-263
A social strategy was tested for implementing Newcastle disease (ND) vaccination and biosecurity improvements among free-ranging chicken at village level in Tanzania. In addition to training the local poultry vaccinators, data recorders and poultry-keepers, the strategy involved training and empowering leaders at the district, ward and village level. The trainings covered poultry health, management, and marketing of village chickens, with an emphasis on ND vaccination and improving biosecurity against avian influenza (AI), The study sites included villages in one ward in each of three each three districts (Iringa, Mtwara-Mikindani, and Mvomero) of mainland Tanzania. Ninety-six local leaders at district level and 101 leaders at ward levels were trained. In addition, 196 farmers (households) were trained, as well as 86 vaccinators and 26 data recorders. Data recorders were also trained as poultry first aid workers. ND vaccination was conducted by the vaccinators, supervised by their local leaders with technical assistance from veterinarians. A total of 158,343 village chickens were vaccinated in three rounds of vaccination three months apart. The training and empowerment of local leaders and local implementers was the key element for success as it fostered the feeling of local ownership of the program and prevented conflicts with other development activities within the villages. We conclude that most animal health programs will increase their odds of success by involving local leaders and by addressing the current challenges facing the farmers. Further assessment on the usefulness of this approach is needed. 相似文献
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116.
ABSTRACT The fungal plant pathogen Microsphaeropsis amaranthi is a candidate bioherbicide for the control of weedy Amaranthus species since it grows and sporulates readily in culture, is a pathogen of a number of important weed species, and is host-restricted to the family Amaranthaceae. This study was designed to determine the optimum and limiting environmental conditions for the efficacy of foliar applications of M. amaranthi for the control of common waterhemp. The greatest disease severity and the greatest plant biomass reductions were found when a prolonged leaf wetness period of >/= 12 h at 18 to 24 degrees C was provided after application. Disease severity and plant biomass reductions were greater when plants were treated at an earlier stage of development. Disease severity and impact were limited when shorter leaf wetness periods were provided, with negligible disease observed on plants provided with =6 h leaf wetness. When common waterhemp seedlings were sprayed in the field weekly through the summer of 2004 with conidial suspensions of M. amaranthi, only 7 of 14 applications resulted in disease and only 3 applications resulted in more than slight disease flecks. Moderate levels of disease were observed when applications had been made on days with moderate temperatures and leaf wetness duration in excess of 12 h following application. We confirm the pathogenicity of M. amaranthi against common waterhemp and demonstrate that the unformulated pathogen can cause disease in the field. Nonetheless, the weather conditions of the Midwestern United States, where common waterhemp control would be desired, are infrequently conducive to infection. The continued candidacy of M. amaranthi as a bioherbicide for the control of common waterhemp in the Midwest-ern United States will require that its efficacy under suboptimal environmental conditions be improved. 相似文献
117.
Shoji Sonoda Xueyan ShiDunlun Song Youjun ZhangJianhong Li Gang WuYong Liu Ming LiPei Liang David WariMasaya Matsumura Chieka MinakuchiToshiharu Tanaka Tadashi MiyataXiwu Gao 《Pesticide biochemistry and physiology》2012,102(2):142-145
In addition to the allele frequencies of the L1014F and T929I mutations which are involved in nerve-insensitive resistance to a pyrethroid, those of the M918I mutation were examined using field strains obtained in China, Thailand, and Japan during 2009-2011. Results show that the resistance allele frequencies at the L1014F site were 89-100%, 97-100% and 65-85%, respectively, for strains in China, Thailand, and Japan. The respective allele frequencies at the T929I site were 86-100%, 70-97% and 58-84% for Chinese, Thai, and Japanese strains. With low frequencies up to 27%, M918I was found in Japan and China, but not in Thailand. The strain homozygous for the M918I and L1014F mutations was established and its resistance level to a pyrethroid was examined. The strain lacks a portion of the sodium channel gene corresponding to the 3′ portion of exon 18a, intron 18, and the 5′ portion of exon 18b. Nevertheless, the strain showed a similar level of resistance to that which was homozygous for the T929I and L1014F mutations. 相似文献
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