全文获取类型
收费全文 | 3891篇 |
免费 | 242篇 |
国内免费 | 2篇 |
专业分类
林业 | 332篇 |
农学 | 78篇 |
基础科学 | 17篇 |
671篇 | |
综合类 | 643篇 |
农作物 | 170篇 |
水产渔业 | 359篇 |
畜牧兽医 | 1488篇 |
园艺 | 120篇 |
植物保护 | 257篇 |
出版年
2023年 | 40篇 |
2022年 | 68篇 |
2021年 | 102篇 |
2020年 | 126篇 |
2019年 | 155篇 |
2018年 | 149篇 |
2017年 | 115篇 |
2016年 | 134篇 |
2015年 | 94篇 |
2014年 | 141篇 |
2013年 | 201篇 |
2012年 | 261篇 |
2011年 | 324篇 |
2010年 | 169篇 |
2009年 | 155篇 |
2008年 | 261篇 |
2007年 | 265篇 |
2006年 | 221篇 |
2005年 | 205篇 |
2004年 | 186篇 |
2003年 | 195篇 |
2002年 | 145篇 |
2001年 | 31篇 |
2000年 | 20篇 |
1999年 | 36篇 |
1998年 | 27篇 |
1997年 | 17篇 |
1996年 | 16篇 |
1995年 | 14篇 |
1994年 | 18篇 |
1993年 | 11篇 |
1992年 | 18篇 |
1991年 | 19篇 |
1990年 | 17篇 |
1989年 | 12篇 |
1988年 | 15篇 |
1986年 | 12篇 |
1985年 | 9篇 |
1984年 | 15篇 |
1983年 | 6篇 |
1982年 | 8篇 |
1981年 | 7篇 |
1980年 | 6篇 |
1978年 | 6篇 |
1976年 | 5篇 |
1974年 | 6篇 |
1971年 | 6篇 |
1928年 | 5篇 |
1925年 | 6篇 |
1924年 | 6篇 |
排序方式: 共有4135条查询结果,搜索用时 15 毫秒
51.
Shima E. Abdalla Akebe L.K. Abia Daniel G. Amoako Keith Perrett Linda A. Bester Sabiha Y. Essack 《The Onderstepoort journal of veterinary research》2022,89(1)
BackgroundDiarrheagenic E. coli (DEC) strains are a major cause of diarrheal diseases in both developed and developing countries. Healthy asymptomatic animals may be reservoirs of zoonotic DEC, which may enter the food chain via the weak points in hygiene practices.AimWe investigated the prevalence of DEC along the pig production continuum from farm-to-fork.MethodsA total of 417 samples were collected from specific points along the pig production system, that is, farm, transport, abattoir and food. E. coli was isolated and enumerated using Colilert. Ten isolates from each Quanti-tray were selected randomly and phenotypically identified using eosin methylene blue agar selective media. Real-time polymerase chain reaction (PCR) was used to confirm the species and to classify them into the various diarrheagenic pathotypes. Antimicrobial susceptibility was determined against a panel of 20 antibiotics using the Kirby-Bauer disk diffusion method and EUCAST guideline.ResultsThe final sample size consisted of 1044 isolates, of which 45.40% (474/1044) were DEC and 73% (762/1044) were multidrug-resistant. Enteroinvasive E. coli (EIEC) was the most predominant DEC at all the sampling sites.ConclusionThe presence of DEC in food animal production environments and food of animal origin could serve as reservoirs for transmitting these bacteria to humans, especially in occupationally exposed workers and via food. Adherence to good hygienic practices along the pig production continuum is essential for mitigating the risk of transmission and infection, and ensuring food safety. 相似文献
52.
First account of a transient intersex in spotted scat,Scatophagus argus: a marine gonochoristic fish
Mustapha Umar Farouk Zhi Fei Huang Yuang-Qing Assan Daniel Li Guang-Li Jiang Dong-Neng 《Fish physiology and biochemistry》2022,48(4):1011-1023
Fish Physiology and Biochemistry - This study presents the first incidence of intersex associated with testis-ova in spotted scat (Scatophagus argus) reared in a controlled environment. The... 相似文献
53.
Daniel P. Harrison Michael G. Hinton Suzanne Kohin Edward M. Armstrong Stephanie Snyder Frank O'Brien Dale K. Kiefer 《Fisheries Oceanography》2017,26(3):316-335
We have developed a set of tools that operate within an aquatic geographic information system to improve the accessibility, and usability of remote‐sensed satellite and computer‐modeled oceanographic data for marine science and ecosystem‐based management. The tools form the Pelagic Habitat Analysis Module (PHAM), which can be applied as a modeling platform, an investigative aid in scientific research, or utilized as a decision support system for marine ecological management. Applications include fisheries, marine biology, physical and biological oceanography, and marine spatial management. The GIS provides a home for diverse data types and automated tools for downloading remote sensed and global circulation model data. Within the GIS environment, PHAM provides a framework for seamless interactive four‐dimensional visualization, for matching between disparate data types, for flexible statistic or mechanistic model development, and for dynamic application of user developed models for habitat, density, and probability predictions. Here we describe PHAM in the context of ecosystem‐based fisheries management, and present results from case study projects which guided development. In the first, an analysis of the purse seine fishery for tropical tuna in the eastern Pacific Ocean revealed oceanographic drivers of the catch distribution and the influence of climate‐driven circulation patterns on the location of fishing grounds. To support management of the Common Thresher Shark (Alopias vulpinus) in the California Current Ecosystem, a simple empirical habitat utilization model was developed and used to dynamically predict the seasonal range expansion of common thresher shark based on oceanographic conditions. 相似文献
54.
Krzysztof Kupren Katarzyna Paliska‐arska Sawomir Krejszeff Daniel arski 《Aquaculture Research》2019,50(9):2528-2536
The morphological development and allometric growth patterns of Eurasian perch, Perca fluviatilis L., a highly valued commercial species, were studied under intensive rearing conditions from hatching up to 50 DPH (Days Post Hatch). Based on the external morphology, four different phases during early development of Eurasian perch were identified: pre‐flexion larva 0–20 DPH (5.70–10.16 mm TL); flexion larva 22–30 DPH (11.09–15.14 mm TL) and post‐flexion larva/juvenile 32–50 DPH (18.00–24.75 mm TL). The results indicate that growth period when final replacement of all temporary (larval) structures and most important changes in the shape of P. fluviatilis occurred (between 13.95 and 24.06 mm TL, during flexion and post‐flexion phase) can be considered as a transitional period between the larva and juvenile. All body segments, except trunk length and tail length showed fast growth (positive allometry) throughout the entire studied period or up to the respective inflexion point with a common tendency to isometry. In addition, the specific behaviours (e.g. pelagic way of life) of Eurasian perch larvae resulted in some characteristic allometric growth patterns in the posterior region, different from the majority of other teleosts. The results are discussed with respect to the ontogeny of the functional morphology under both ecological and aquaculture considerations. 相似文献
55.
The application of tannic acid to the elimination of egg stickiness at varied moments of the egg swelling process in pikeperch,Sander lucioperca (L.) 下载免费PDF全文
Daniel Żarski Sławomir Krejszeff Dariusz Kucharczyk Katarzyna Palińska‐Żarska Katarzyna Targońska Krzysztof Kupren Pascal Fontaine Patrick Kestemont 《Aquaculture Research》2015,46(2):324-334
The aim of this article was to analyse the process of pikeperch, Sander lucioperca, egg swelling and to apply tannic acid to eliminate egg stickiness at different moments of the swelling process on artificially obtained eggs. The first experiment involved observation of egg swelling process and the second determined the effect of temperature (12, 14 and 16°C) on the egg swelling rate. The third experiment involved elimination of egg stickiness in a tannin solution (0.75 g L?1) where eggs were submerged in a solution for 0.5, 1, 2 and 5 min – 5, 10, 15, 20, 25 and 30 min following gamete activation. The results indicate that the pikeperch egg swelling process lasts 30 min. It was found that the temperature did not affect the process duration. The results of the third experiment showed that the effectiveness of tannic acid application in egg stickiness elimination increases with time. The best result was obtained in groups of eggs submerged for 1 and 2 min (86.5% and 80.5% of larvae were obtained respectively) 30 min following the gamete activation. The results presented in this study for the first time indicate the possibility of highly effective procedure of egg stickiness elimination with tannic acid in pikeperch aquaculture. 相似文献
56.
Assessment of gut microbiota in different developmental stages of Malaysian Mahseer (Tor tambroides) 下载免费PDF全文
Mohamad Zaimin Mohd Nosi Sharifah Noor Emilia Syed Jamil Fadaak Muhd Danish Daniel Muhammad Shumpei Iehata 《Aquaculture Research》2018,49(9):2977-2987
Malaysian Mahseer (Tor tambroides) has a good prospect for aquaculture because of its high market demand. However, there is a scarce information on gut microbiota associated with Malaysian Mahseer unlike other fish species. Therefore, we constructed and compared gut microbiota in different developmental stages (larval, juvenile, fingerling, yearling, and adult) using culture dependent and PCR‐DGGE fingerprinting technique for better understanding of gut microbiota composition associated with T. tambroides. Culturable gut microbiota composition in all developmental stages were composed of β‐ and γ‐Proteobacteria, and Bacilli. Biodiversity analysis of culturable gut microbiota showed that larval, juvenile, and adult stages have higher diversity than fingerling and yearling stages. Ward's linkage cluster analysis showed that culturable gut microbiota composition in larval and juvenile stages were close to adult stages, whereas fingerling and yearling stage composed same cluster. PCR‐DGGE fingerprinting technique showed that unculturable gut microbiota were constituted by α‐and γ‐Proteobacteria, and Actinobacteria. Ward's linkage cluster analysis showed that unculturable gut microbiota composition in both larval and juvenile stages were distinct from other developmental stages. Our results revealed that gut microbiota composition were varied in different developmental stages of Malaysian Mahseer and continuous shifts of gut microbiota from larval to adult stages. 相似文献
57.
A qPCR assay was developed for rapid and sensitive detection of Flavobacterium psychrophilum, the aetiological agent of bacterial cold-water disease and rainbow trout fry syndrome in salmonid fish worldwide. A set of F. psychrophilum-specific primers based on 16S rRNA gene sequences was designed and validated for specific detection and quantification of DNA isolated from representative strains of F. psychrophilum. The qPCR assay exhibited a high specificity for the 16S rRNA gene of F. psychrophilum (from 4 × 10(8) down to 11 copies per reaction) but not for other Flavobacterium species or other bacteria including fish pathogens. This qPCR-based method proved to be useful in the quantification of the F. psychrophilum titre present within organs dissected out from diseased fish. As the F. psychrophilum genome contains six copies of the 16S rRNA gene, we could infer a limit of detection corresponding to two bacteria per reaction, corresponding to 800 bacteria per fish tissue sample, and therefore 20 F. psychrophilum cells mg(-1) of tissue (for sample weighing 40 mg). The qPCR assay reported here could be a useful tool for veterinary diagnostic laboratories to monitor the F. psychrophilum infection level in fish farms. 相似文献
58.
Marine fisheries target and catch fish both for direct human consumption (DHC) as well as for fishmeal and fish oil, and other products. We derived the fractions used for each for 1950–2010 by fishing country, and thus provide a factual foundation for discussions of the optimal use of fisheries resources. From 1950 to 2010, 27% (~20 million tonnes annually) of globally reconstructed marine fisheries landings were destined for uses other than DHC. Importantly, 90% of fish destined for uses other than DHC are food‐grade or prime food‐grade fish, while fish without a ready market for DHC make up a much smaller proportion. These findings have implications for how we are using fish to feed ourselves or, more appropriately, how we are not using fish to feed ourselves. 相似文献
59.
- 1. Conservation and rehabilitation efforts for lake sturgeon Acipenser fulvescens throughout the Great Lakes include the re‐establishment of self‐sustaining stocks in systems where they have been extirpated.
- 2. Information on the suitability of potential lake sturgeon habitat in tributaries is important for determining their capacity to support lake sturgeon stocking and to develop system‐specific rehabilitation strategies.
- 3. Geo‐referenced habitat information characterizing substrate composition, water depth, and stream gradient were applied to a life‐stage specific lake sturgeon habitat suitability index in a geographic information system to produce spatially explicit models of life‐stage specific habitat characteristics in five northern Lake Michigan tributaries from which lake sturgeon have been extirpated.
- 4. Habitat models indicated that high quality lake sturgeon spawning and staging habitat comprised 0 to 23% and 0 to 9% of the available habitat, respectively, whereas high quality juvenile lake sturgeon habitat was relatively ubiquitous throughout each river and comprised 39 to 99%.
- 5. Comparison of these data to lake sturgeon habitat availability in Lake Michigan tributaries currently supporting populations indicated that spawning and staging habitats may limit the ability of these systems to support spawning. Efforts to re‐establish lake sturgeon populations in these systems should consider the creation of spawning and staging habitat to increase reproductive and recruitment potential prior to the initiation of stocking efforts.
60.
Thomas H Hansen Kristian H Laursen Daniel P Persson Pai Pedas Søren Husted Jan K Schjoerring 《Plant methods》2009,5(1):12-11