四川猪2型链球菌病的PCR检测

2005年7月中下旬,四川贵阳等地人、猪陆续发生一种发病惠、死亡快、高度散发的疾病,从病死猪组织中分离出19株链球菌,设计合成3对引物,分别对分离菌株进行了猪链球菌16SrRNA、CPS2J、MRP基因片段扩增,有15株菌3个基因的扩增结果均为阳性,表明此次疲情的病原为猪2型链球菌。     

An investigation of Leishmania spp. in Didelphis spp. from urban and peri-urban areas in Bauru (São Paulo, Brazil)

Blood and bone marrow samples were taken from 112 Didelphis spp., collected between March 2005 and February 2006, from urban and peri-urban areas of Bauru, São Paulo State, Brazil, to evaluate the hypothesis that these animals might constitute a reservoir of Leishmania spp. Anti-Leishmania ssp. antibodies were screened in the serum samples using an enzyme-linked immuno-sorbent assay (ELISA) and the polymerase chain reaction (PCR). PCR was performed on fragments of DNA samples from Leishmania spp. using primers 13A and 13B, and showed a positive outcome in 91.6% of the 112 samples tested. Of the 107 samples analyzed by ELISA, 71% were positive. Evidence of epidemiological risk factors such as a circulating parasite and freely moving vectors suggests that Didelphis spp. may participate in the transmission cycle of Leishmania spp. in Bauru.     

不同饲养方式中蜂形态学初探

对饲养在圆木桶、墙洞、活框蜂箱中10年以上的中蜂蜂群进行工蜂标准采集,测定其工蜂的40个形态指标,对比分析吻长等9个与生产力相关的指标,结果表明,对圆木桶、墙洞、活框蜂箱三种饲养方式进行人为干预,能使中蜂蜂群与生产力相关的形态指标得到优化。     

用SAS的mixed过程拟合林分的线性差分生长模型

本研究的目的在于研究如何用SAS的proc mixed过程拟合线性代数差分模型。所用数据来源于148个集约经营火炬松人工林。直接拟合了一个胸高断面积的收获模型,而非代数差分生长模型。模型拟合过程如下:i).同时确定随林分变化的参数和最优拟合的方差结构模型;ii).依据AIC、BIC和极大似然比检验化简期望模型;iii).用代数差分法将拟合的收获模型转化为代数差分生长模型。     

兽药的合理使用

科学、高效、安全地使用兽药,不但能及时预防和治疗动物疾病,提高养殖效益,而且对控制和减少药物残留、提高动物产品质量具有重要意义。     

季节对山羊反刍行为的影响

对１６只中卫山羊母羊的青草期和枯草期反刍行为进行观察表明，山羊在青草期，每天（２４ｈｒｓ）有１０个反刍周期，反刍持续总时间为３０４．２分，共反刍４２５．３次，每个食丸在口腔中咀嚼４３．０秒，共咀嚼４６．４次，在枯草期上述指标分别为１１个，４２０．０分，４７２．０次，５３．４秒和５９．６次，季节对山羊反刍行为的影响，本质上决定于羊采食牧草的品质和数量，优质牧草（青草期）能够缩短反刍持续总时间（Ｐ〉０     

参加央视《中国工作犬大集合》节目后两警犬再立新功

参加完中央电视台《中国工作犬大集合》节目后的工作犬,他们回到各自的岗位上,有什么突出表现呢?     

牛支原体和牛病毒性腹泻病毒二重二温式PCR检测方法的建立

为建立一种牛支原体(Mycoplasma bovis,MB)和牛病毒性腹泻病毒(Bovine viral diarrhea virus,BVDV)的快速鉴别诊断方法,针对MB的uvr C基因和BVDV的5'端非编码区(5'-UTR)保守基因序列,分别设计两对特异引物,并将三温式PCR扩增程序简化为二个温度梯度,建立了鉴别MB和BVDV的二重二温式PCR方法。该方法能同时扩增MB和BVDV,扩增产物大小分别为412和170 bp。特异性试验结果显示,该方法对参试的所有毒株只扩增MB和BVDV基因组,对其它牛病原体无扩增;敏感性试验结果显示,该方法最低能同时检测到104拷贝的两种目的核酸;干扰性试验结果显示,该方法能同时检测两个模板不同浓度的组合,试验结果不受模板影响。综上,本研究所建立的二重二温式PCR方法特异、敏感、快速、简便,可应用于MB和BVDV临床鉴别诊断和流行病学调查。     

Gene cloning of porcine adiponectin gene from adipose tissue and construction of its eukaryotic expression vector

To clone adiponectin (ADPN) gene from Shaziling porcine adipocyte and construct its eukaryotic expression vector, total RNA was extracted from subcutaneous fatty tissue. One pair of specific primers was designed by Primer 5.0 software according to the sequence of ADPN gene of porcine available in GenBank. The ADPN gene was amplified by PCR from cDNA and cloned into pMD18‐T vector to construct recombinant clonal vector pMD‐ADPN, sequenced and analysed. A recombinant expression plasmid pPICZaA‐ADPN was constructed by subcloning the cloned ADPN gene into the linearized pPICZaA vector. Then, the plasmid pPICZaA‐ADPN was expressed in Pichia pastoris (GS115) by electrotransformation. Western blot and Bradford analysis were used to determine the target protein induced by methanol. Results showed that the genome size of ADPN was 732 bp and encoded 244 amino acid, the nucleotide sequence of ADPN shared 100% identity with that of porcine available in GenBank. Western blot and Bradford analysis showed that the recombinant ADPN was expressed in GS115 correctly and has certain immune activity. The expression level of ADPN was 28.5 μg/ml. In conclusion, the recombinant ADPN could express in eukaryotic expression vector pPICZaA‐ADPN constructed in this study effectively.