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51.
Development of an enzyme-linked immunosorbent assay for the detection of the fungicide fenarimol 总被引:3,自引:0,他引:3
Lee JK Park SH Lee EY Kim YJ Kyung KS 《Journal of agricultural and food chemistry》2004,52(24):7206-7213
To develop an enzyme-linked immunosorbent assay for the fungicide fenarimol, two synthesized haptens, haptens-1 and -2, and the purchased 4,4'-DDA were conjugated to carrier proteins (BSA, KLH, and OVA). Polyclonal antibodies raised against hapten-1,2-KLH conjugates in rabbits and the coating antigens of hapten-1,2-BSA conjugates, hapten-2-OVA conjugate, and 4,4'-DDA-BSA conjugate were screened and selected for the homologous and/or heterologous ELISA formats. Two competitive indirect ELISAs were selected: assays I and II. The optimized ciELISAs of assays I and II showed average IC(50) values of fenarimol of 5.4 and 9.4 ng/mL, detection ranges of 1.1-25.9 and 1.1-82.7 ng/mL, and lowest detection limits of 0.3 and 0.3 ng/mL, respectively. The cross-reactivities with several structurally related compounds indicated the importance of the steric fitness in the antigen-antibody interaction. Recoveries of fenarimol from apple and pear samples spiked with the analyte by assay I were in the range of 93-113% by simple extraction, concentration, and dilution. This assay could be a convenient and supplemental analytical tool for monitoring fenarimol residues in environmental and agricultural samples. 相似文献
52.
53.
The aim of this study was to investigate the characteristics and oxidative stability of chitosan-glutathione conjugate (CS-GSH) and CS-GSH nanoparticles (CS-GSH NPs) to explore the potentials of these nanoparticle systems for GSH delivery. CS-GSH was synthesized using a radical polymerization method, and CS-GSH NP was prepared by ionic gelation of CS-GSH with sodium tripolyphosphate (TPP). The sizes of CS-GSH NPs significantly increased with increasing CS-GSH concentration and CS-GSH/TPP ratio. The entrapment efficiency (EE) significantly increased with increasing CS-GSH concentration and significantly decreased with increasing CS-GSH/TPP ratio. The immobilized GSH could be protected against oxidation compared to free GSH. The thiol content in the nanoencapsulated GSH was more effectively maintained than those in free GSH and CS-GSH, regardless of the presence of oxidative stress-inducing agents. These results suggest that CS-GSH NP can be used to enhance the oxidative stability of GSH. 相似文献
54.
Lee CH Yoo KY Choi JH Hwang IK Choi SY Won MH 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(3):361-365
Ribosomal protein S3 (rpS3) is well known to participate in DNA repair mechanisms. In the present study, we compared rpS3 immunoreactivity and its protein levels in the hippocampus among young, adult and aged gerbils. In the postnatal month (PM) 3 group as the young, rpS3 immunoreaction was observed in pyramidal and non-pyramidal cells of the hippocampus proper and in granule and polymorphic cells of the dentate gyrus. In the PM 12 group as adult and 24 group as the aged, rpS immunoreactivity in the hippocampus was decreased compared to the PM 3 group. Western blot analysis showed that rpS3 levels were decreased with time; lowest at PM 24. These results indicate that rpS3 immunoreactivity and protein levels were markedly decreased in the aged gerbil hippocampus. 相似文献
55.
Kang-Woong Kim Sungchul C. Bai Ja-Wan Koo Xiaojie Wang Se-Kwon Kim 《Journal of the World Aquaculture Society》2002,33(4):425-431
The study was conducted to investigate the effects of dietary Chlorella powder (C) supplementation on growth performance, blood characteristics, and whole-body composition in juvenile Japanese flounder Paralichthys olivaceus . Four experimental diets were supplemented with C at 0, 1, 2 and 4% (C0 , C1 , C2 , and C4 ) on a dry-weight basis. Three replicate groups of fish averaging 1.13 ± 0.02 g (Mean ± SD) were randomly distributed in each aquarium and fed one of four experimental diets for 12 wk. After 12 wk of the feeding trial, fish fed C2 diet had higher weight gain (WG), feed efficiency (FE), and protein efficiency ratio (PER) than did fish fed C0 and C1 diets ( P 0.05); however, there was no significant difference among fish fed C1 and C4 diets, and among fish fed C2 and C4 diets ( P > 0.05). Fish fed C2 and C4 diets had a lower serum cholesterol level than did fish fed C0 and C1 diets ( P 0.05). Fish fed C2 and C4 diets had a lower body fat than did fish fed C0 ( P 0.05). These results indicate that dietary supplementation of 2% Chlorella powder in the commercial diets could improve growth, feed utilization, serum cholesterol level, and whole-body fat contents in juvenile Japanese flounder. 相似文献
56.
Ok Jae Koo Seung-Kwon Ha Sol Ji Park Hee Jung Park Su Jin Kim Daekee Kwon Jung Taek Kang Joon Ho Moon Eun Jung Park Goo Jang Byeong Chun Lee 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(2):241-244
Somatic cell nuclear transfer (SCNT) is a cost-effective technique for producing transgenic pigs. However, abnormalities in the cloned pigs might prevent use these animals for clinical applications or disease modeling. In the present study, we generated several cloned pigs. One of the pigs was found to have intrapancreatic ectopic splenic tissue during histopathology analysis although this animal was grossly normal and genetically identical to the other cloned pigs. Ectopic splenic tissue in the pancreas is very rare, especially in animals. To the best of our knowledge, this is the first such report for cloned pigs. 相似文献
57.
58.
Young Seok Koo 《Fibers and Polymers》2001,2(3):148-152
Cotton yarns were coated with a polymer solution to hold surface fibers to the yarn body, which caused fiber-fly generation
during knitting process. The physical property of the coated yarn, especially a bending rigidity was investigated in order
to evaluate the performance of the coated yarn during knitting. SEM images showing the surface condition of the coated yarn
demonstrated that the thickness of a coating material increased as the concentration of the coating solution increased. The
results of the bending rigidity measured using KES-FB2 system showed that the bending rigidity of the coated yarn increased
as the concentration of the coating solution increased. The results also revealed the possibility that yarn coated with a
low amount of coating material should be employed for further research of reducing fiber-fly generation during knitting process. 相似文献
59.
The effects of opening, carding, and repeated drawings on single fiber and bundle cotton characteristics were studied by employing Mantis®, AFIS® and HVI Testers. Some of the significant changes in single fiber properties were found to be due to process parameters as well as the changes in the fiber crimps, parallelness of fibers within HVI beards, and the actual changes in the tensile properties of the fibers. The study showed that the HVI test data taken just prior to spinning had the highest correlation with the yarn tensile properties. Based on the study results, we point out the potential of HVI for future quality and process control in spinning by recommending a set of expanded HVI output that is more scientific and comprehensive for the future control needs. 相似文献
60.
Using conventional soil and foliar spray applications to correct micronutrient deficiency in citrus has not been completely satisfactory. Therefore, this study was developed to test the effectiveness of micronutrient application through microirrigation (fertigation) at rates similar to those recommended for foliar spray application. Three field experiments were conducted on ‘Valencia’ orange [Citrus sinensis (L.) Osb.] trees to study the absorption of Fe, Mn, Zn, and Cu by periodically analyzing leaf samples. The effectiveness of fertigation with micronutriems was found to depend on the fertilizer source. Application of chelated Fe, Mn, and Zn through irrigation systems increased the concentrations of these micronutrients in the leaves. The nitrate forms of Fe, Mn, and Zn were ineffective, as was the sulfate form of Zn. The sulfate form of Mn was occasionally effective, but the sulfate form of Cu was very effective. The application of chelated micronutrients through fertigation shows promise in central Florida. 相似文献